Hotspot

rps12-clpP

LSC · 75397-75761 · 365 bp

Back to Abelia

Pi 0.0348
MarkerSeek score 81.00
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
365
variable_sites
38
pi
0.0348
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 GTACATGTTCCTCGACGCTG ACCAAACGTCTAGCATTCCC 55.0 50.0 58.7 57.9 559-575 bp 1.0 82.6
rps12-clpP_p2 GTACATGTTCCTCGACGCTG GAAGACTATGCCTTCGCCAT 55.0 50.0 58.7 57.8 498-514 bp 1.0 82.2
rps12-clpP_p3 AGTACATGTTCCTCGACGCT ACCAAACGTCTAGCATTCCC 50.0 50.0 58.8 57.9 560-576 bp 1.0 81.9
rps12-clpP_p4 TACATGTTCCTCGACGCTGA ACCAAACGTCTAGCATTCCC 50.0 50.0 58.8 57.9 558-574 bp 1.0 81.9
rps12-clpP_p5 TAGTACATGTTCCTCGACGC ACCAAACGTCTAGCATTCCC 50.0 50.0 57.2 57.9 561-577 bp 1.0 81.8