Hotspot

trnH-GUG-psbA

LSC · 234-593 · 360 bp

Back to Abelia

Pi 0.0446
MarkerSeek score 74.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
360
variable_sites
47
pi
0.0446
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA CGGATGTAGCCAAGTGGATC 50.0 55.0 57.9 58.1 210-235 bp 1.0 89.9
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TGGATCAAGGCAGTGGATTG 55.0 50.0 58.1 57.9 182-207 bp 1.0 89.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CGGATGTAGCCAAGTGGATC 50.0 55.0 57.9 58.1 209-234 bp 1.0 89.9
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG GGATCAAGGCAGTGGATTGT 55.0 50.0 58.1 57.9 181-206 bp 1.0 89.9
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG GTGGATCAAGGCAGTGGATT 55.0 50.0 58.1 57.9 183-208 bp 1.0 89.9