Hotspot

rpl32-trnL-UAG

SSC · 111133-111835 · 703 bp

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Pi 0.051
MarkerSeek score 74.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
703
variable_sites
106
pi
0.051
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.60

Pi Curve

Alignment Viewer

                         1                                                                                                   
Salvia_aethiopis_1       GTTATTAAGAAATAAAGCGG-AAACCTTAGAAC---------CGACCTGATTCAAAAATGGAACCCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_amarissima_2      GTTATTAAGAAATAAAGCGGAAAACCTTACAACAATATAAATCGACCTGACTCAAAAATGGAATCCTTCCATTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_chinensis_3       GTTATTAAGAAATAAAGCGGAAAACCTTAGAACAATATAAATCGACCTGACTCAAAAATGGAACGCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_dorrii_4          GTTATTAAGAAATAAAGCGGAAAACCTTAGAAGAATATAAATCGACCTGACTCAAAAATTGAACCCTTCCATTTC-AAAAAAAATTCCCCAATTCCATTT
Salvia_eremostachya_5    GTTATTAAGAAATAAAGCGGAAAACCTTAGAACAATATAAATCGACCTGACTCAAAAATTGAACCCTTCCATTTC-AAAAAAAATTCCCCAATTCCATTT
Salvia_fruticosa_6       GTTATTAAGAAATAAAGCGG-AAACCTTAGAACAATATAAATCGACCTGATTCAAAAATGGAACCCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_huberi_7          GTTATTAAGAAATAAAGCGG-AAACCTTAGAACAATATAAATCGACCTGATTCAAAAATGGAACCCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_kronenburgii_8    GTTATTAAGAAATAAAGCGG-AAACCTTAGAACAATATAAATCGACCTGATTCAAAAATGGAACCCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_limbata_9         GTTATTAAGAAATAAAGCGG-AAACCCTAGAAC---------CGACCTGATTCAAAAATGGAACCCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_mohavensis_10     GTTATTAAGAAATAAAGCGGAAAACCTTAGAACAATATAAATCGACCTGACTCAAAAATTGAACCCTTCCATTTC-AAAAAAAATTCCCCAATTCCATTT
Salvia_officinalis_subsp GTTATTAAGAAATAAAGCGG-AAACCTTAGAACAATATAAATCGACCTGATTCAAAAATGGAACCCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_pachyphylla_12    GTTATTAAGAAATAAAGCGGAAAACCTTAGAACAATATAAATCGACCTGACTCAAAAATGGAACCCTTCCATTTC-AAAAAAAATTCCCCAATTCCATTT
Salvia_pratensis_13      GTTATTAAGAAATAAAGCGG-AAACCCTAGAAC---------CGACCTGATTCAAAAATGGAACCCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_saccardiana_14    GTTATTAAGAAATAAAGCGG-AAACCCTAGAAC---------CGACCTGATTCAAAAATGGAACCCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_sonomensis_15     GTTATTAAGAAATAAAGCGGAAAACCTTAGAACAATATAAATCGACCTGACTCAAAAATGGAACCCTTCCATTTC-AAAAAAAATTCCCCAATTCCATTT
Salvia_spathacea_16      GTTATTAAGAAATAAAGCGGAAAACCTTAGAAGAATATAAATCGACCTGACTCAAAAATTGAACCCTTCCATTTC-AAAAAAAATTCCCCAATTCCATTT
Salvia_verbenaca_17      GTTATTAAGAAATAAAGCGG-AAACCCTAGAAC---------CGACCTGATTCAAAAATGGAACCCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
Salvia_xanthocheila_18   GTTATTAAGAAATAAAGCGG-AAACCCTAGAAC---------CGACCTGATTCAAAAATGGAACCCTTCCGTTTCAAAAAAAAATTCCCCAATTCCATTT
                         101                                                                                                 
Salvia_aethiopis_1       CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACCAAATTTAAACAAAGTTTTATCAATACAAAAAAATACAAGATACTAGATTTA
Salvia_amarissima_2      CCTATTGATTAATTCACCAGGAAATGGAATTCTTCTGTTTACTTTGACCGAATTCAAACAAAGTTTTTTTAAC-AAAAAAAACACAAGATACTCGATTTC
Salvia_chinensis_3       CCTGGTAAATTAATCAATGGGAAATGGAATTCTTCTGGTTACTTTGACCGAATTCAAACAAAGTTTTTTTAACAAAAAAAAACACAAGATACTTGATTTA
Salvia_dorrii_4          CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACTGAATTCAAACAAAGTTTTTTTTAACAAAAAAAACACAAGATACTCAATTTA
Salvia_eremostachya_5    CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACTGAATTCAAACAAAGTTTTTTTAACAAAAAAAAACACAAGATACTCAATTTA
Salvia_fruticosa_6       CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACCTAATTTAAACAAAGTTTTTTT---ACAAAAAAATACAAGATACTCGATTTA
Salvia_huberi_7          CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACCTAATTTAAACAAAGTTTTTTT---ACAAAAAAATACAAGATACTCGATTTA
Salvia_kronenburgii_8    CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACCTAATTTAAACAAAGTTTTTTT---ACAAAAAAATACAAGATACTCGATTTA
Salvia_limbata_9         CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACCAAATTTAAACAAAGTTTTATCAATACAAAAAAATACAAGATACTAGATTTA
Salvia_mohavensis_10     CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACTGAATTCAAACAAAGTTTTTTTTAACAAAAAAAACACAAGATACTCAATTTA
Salvia_officinalis_subsp CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACCTAATTTAAACAAAGTTTTTTT---ACAAAAAAATACAAGATACTCGATTTA
Salvia_pachyphylla_12    CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACTGAATTCAAACAAAGTTTTTTTTAACAAAAAAAACACAAGATACTCAATTTA
Salvia_pratensis_13      CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACCAAATTTAAACAAAGTTTTATCAATACAAAAAAATACAAGATACTAGATTTA
Salvia_saccardiana_14    CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACCAAATTTAAACAAAGTTTTATCAATACAAAAAAATACAAGATACTAGATTTA
Salvia_sonomensis_15     CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACTGAATTCAAACAAAGTTTTTTTTAACAAAAAAAACACAAGATACTCAATTTA
Salvia_spathacea_16      CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACTGAATTCAAACAAAGTTTTTTTTAACAAAAAAAACACAAGATACTCAATTTA
Salvia_verbenaca_17      CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACCAAATTTAAACAAAGTTTTATCAATACAAAAAA-------ATACTAGATTTA
Salvia_xanthocheila_18   CCTGGTGAATTAATCAATAGGAAATGGAATTCTTCTGTTTACTTTGACCAAATTTAAACAAAGTTTTATCAATACAAAAAAATACAAGATACTAGATTTA
                         201                                                                                                 
Salvia_aethiopis_1       TATTTTAGTATATTTTGTTTCCAGGATGGGAGTCTTTTTTTCCCATCAATCTATTTGTAATATA--TCTTTTTTGTACAACTTGCTTACTTTTGGATTTT
Salvia_amarissima_2      AATTTTCGTATATTTTATTTCCAGGACGGGAGTCTTTTTTTCCCATCAACCTATTTGTACTATAAATATTTTTTGTACAACTTTCTTACTTTTGAATTTC
Salvia_chinensis_3       AATTTTCGTATATTTTCTTGCCAGGACGGGAGTCTTTTTTTCCCATCAATCTATTTGTACTATAAATATTTTTTGCACAACTTTCTTACTTTTTAATTTC
Salvia_dorrii_4          AATTTGAGTATATTTTCTTTCCAGGACGGGAGTCTTTTTTTCCCATCAACCTATTTGTACTATAAATATTTTTTGTACAACTTTCTTACTTTTGAATTTC
Salvia_eremostachya_5    AATTTGAGTATATTTTCTTTCCAGGACGAGAGTCTTTTTTTCCCATCAACCTATTTGTACTATAAATATTTTTTGTACAACTTTCTGACTTTTGAATTTC
Salvia_fruticosa_6       TATTTTAGTATATTTTCTTTCCAGGATGGGAGTCTTTTTTTCCCATCAATCTATTTGTACTATA--CCTTT-TTGTACAACTTGCTTACTTTTGGATTTT
Salvia_huberi_7          TATTTTAGTATATTTTCTTTCCAGGATGGGAGTCTTTTTTTCCCATCAATCTATTTGTACTATA--CCTTT-TTGGACAACTTGCTTACTTTTGGATTTT
Salvia_kronenburgii_8    TATTTTAGTATATTTTCTTTCCAGGATGGGAGTCTTTTTTTCCCATCAATCTATTTGTACTATA--CCTTT-TTGGACAACTTGCTTACTTTTGGATTTT
Salvia_limbata_9         TATTTTAGTATATTTTGTTTCCAGGATGGGAGTCTTTTTTTCCCATCAATCTATTTGTAATAGA--TCTTTTTTGTACAACTTGCTTACTTTTGGATTTT
Salvia_mohavensis_10     AATTTTAGTATATTTTCTTTCCAGGACGGGAGTCTTTTTTTCCCATCAACCTATTTGTACTATAAATATTTTTTGTACAACTTTCTTACTTTTGAATTTC
Salvia_officinalis_subsp TATTTTAGTATATTTTCTTTCCAGGATGGGAGTCTTTTTTTCCCATCAATCTATTTGTACTATA--CCTTT-TTGTACAACTTGCTTACTTTTGGATTTT
Salvia_pachyphylla_12    AATTTTAGTATATTTTCTTTCCAGGACGGGAGTCTTTTTTTCCCATCAACCTATTTGTACTA--------------------------------------
Salvia_pratensis_13      TATTTTAGTATATTTTGTTTCCAGGATGGGAGTCTTTTTTTCCCATCAATCTATTTGTAATATA--TCTTTTTTGTACAACTTGCTTACTTTTGGATTT-
Salvia_saccardiana_14    TATTTTAGTATATTTTGTTTCCAGGATGGGAGTCTTTTTTTCCCATCAATCTATTTGTAATATA--TCTTTTTTGTACAACTTGCTTACTTTTGGATTT-
Salvia_sonomensis_15     AATTTTAGTATATTTTCTTTCCAGGACGGGAGTCTTTTTTTCCCATCAACCTATTTGTACTA--------------------------------------
Salvia_spathacea_16      AATTTGAGTATATTTTCTTTCCAGGACGGGAGTCTTTTTTTCCCATCAACCTATTTGTACTATAAATATTTTTTGTACAACTTTCTTACTTTTGAATTTC
Salvia_verbenaca_17      TATTTTAGTATGTTTTGTTTCCAGGATGGGAGTCTTTTTTTCCCATCAATCTATTTGTAATATA--TCTTTTTTGTACAACTTGCTTACTTTTGGATTTT
Salvia_xanthocheila_18   TATTTTAGTATATTTTGTTTCCAGGATGGGAGTCTTTTTTTCCCATCAATCTATTTGTAATATA--TCTTTTTTGTACAACTTGCTTACTTTTGGATTTT
                         301                                                                                                 
Salvia_aethiopis_1       TATATATTCTTATATAGAGCCCCTATAGCTCTCACCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTGGAAAAAT
Salvia_amarissima_2      TATAAATTCTTATATAGAGCCGATATATCTCTCGCCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTTGAATAAT
Salvia_chinensis_3       TATAAATTCTTATATAGAGCCCATATATCTCTCCCCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATGGGTGTGAATTTTGAATAAT
Salvia_dorrii_4          TATAAATTCTTATATAGAGCCTATATATCTCTCGCCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTTGAATAAT
Salvia_eremostachya_5    TATAAATTCTTATATAGAGCCCATATATCTCTCGCCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTTGAATAAT
Salvia_fruticosa_6       TATAAATTCTTATATAGAGCCCATATAGCTCTCGCCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTGGAAAAAT
Salvia_huberi_7          TATAAATTCTTATATAGAGCCCATATAGCTCTCGCCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTGGAAAAAT
Salvia_kronenburgii_8    TATAAATTCTTATATAGAGCCCATATAGCTCTCGCCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTGGAAAAAT
Salvia_limbata_9         TATATATTCTTATATAGAGCCCCTATAGCTCTCACCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTGGAAAAAT
Salvia_mohavensis_10     TATAAATTCTTATATAGAGCCTATATATCTCTCGCCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTTGAATAAT
Salvia_officinalis_subsp TATAAATTCTTATATAGAGCCCATATAGCTCTCGCCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTGGAAAAAT
Salvia_pachyphylla_12    ----------------------------------------------------------------------------------------------------
Salvia_pratensis_13      TATATATTCTTATATAGAGCCCCTATAGCTCTCACCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTGGAAAAAT
Salvia_saccardiana_14    TATATATTCTTATATAGAGCCCCTATAGCTCTCACCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTGGAAAAAT
Salvia_sonomensis_15     ----------------------------------------------------------------------------------------------------
Salvia_spathacea_16      TATAAATTCTTATATAGAGCCTATATATCTCTCGCCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTTGAATAAT
Salvia_verbenaca_17      TATATATTCTTATATAGAGCCCCTATAGCTCTCACCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTGGAAAAAT
Salvia_xanthocheila_18   TATATATTCTTATATAGAGCCCCTATAGCTCTCACCATCAATTCACGCAAAAACACTTAATGAAAATATTCTGGATAAATATGTGTGAATTTGGAAAAAT
                         401                                                                                                 
Salvia_aethiopis_1       CTAAAATATTTTTTTGTTTATTGATAAAACTTATTTTTGGGTTGTACTTTTTAAAATGAAAATCAAATAAATCAAAAACGGTAAATTAAAA---------
Salvia_amarissima_2      CTAAAATATTTTTTTT------------------------------------------------------------------------------------
Salvia_chinensis_3       CTAAAATCTTTTTTGGTTCATTCATAAAACAGATTTTTGGGTTGTACTTTTTGAAATTAAAATCAAATAACTCAAAAACGGTAAATTTTAAAAAATGTTT
Salvia_dorrii_4          CTAAAATATTTTTTTGTTCATTTATAAAACTCATTTTGGGGTTGTACTTTTTAAAATTAAAATCAAATAACTTAAAAACGGTAAATAAAAAAATGTTTTT
Salvia_eremostachya_5    CTAAAATATTTTTTTGTTCATTCATAAAACTCATTTTGGGGTTGTACTTTTTAAAATGAAAATCAAATAACTCAAAAACGGTAAATAAAAAAATGTTTTT
Salvia_fruticosa_6       CTAAAATATTTTTTTGTTCATTGATAAAACTTATTTTTGGGTTGTACTTTTTTAAATGAAAATCAAATAAATCAAAAACGGTAAATAAAAA---------
Salvia_huberi_7          CTAAAATATTTTTTTGTTCATTGATAAAACTTATTTTGGGGTTGTACTTTTTTAAATGAAAATCAAATAAATCAAAAACGGTAAATAAAAA---------
Salvia_kronenburgii_8    CTAAAATATTTTTTTGTTCATTGATAAAACTTATTTTTGGGTTGTACTTTTTTAAATGAAAATCAAATAAATCAAAAACGGTAAATAAAAA---------
Salvia_limbata_9         CTAAAATATTTTTTTGTTTATTGATAAAACTTATTTTTGGGTTGTACTTTTTAAAATGAAAATCAAATAAATCAAAAACGGTAAATTAAAA---------
Salvia_mohavensis_10     CTAAAATATTTTTTTGTTCATTTATAAAACTCATTTTGGGGTTGTACTTTTTAAAATTAAAATCAAATAACTTAAAAACGGTAAATAAAAAAATGTTTTT
Salvia_officinalis_subsp CTAAAATATTTTTTTGTTCATTGATAAAACTTATTTTTGGGTTGTACTTTTTTAAATGAAAATCAAATAAATCAAAAACGGTAAATAAAAA---------
Salvia_pachyphylla_12    ----------------------------------------------------------------------------------------------------
Salvia_pratensis_13      CTAAAATATTTTTTTGTTTATTGATAAAACTTATTTTTGGGTTGTACTTTTTAAAATGAAAATCAAATAAATCAAAAACGGTAAATTAAAA---------
Salvia_saccardiana_14    CTAAAATATTTTTTTGTTTATTGATAAAACTTATTTTTGGGTTGTACTTTTTAAAATGAAAATCAAATAAATCAAAAACGGTAAATTAAAA---------
Salvia_sonomensis_15     ----------------------------------------------------------------------------------------------------
Salvia_spathacea_16      CTAAAATATTTTTTTGTTCATTTATAAAACTCATTTTGGGGTTGTACTTTTTAAAATTAAAATCAAATAACTTAAAAACGGTAAATAAAAAAATGTTTTT
Salvia_verbenaca_17      CTAAAATATTTTTTTGTTTATTGATAAAACTTATTTTTGGGTTGTACTTTTTAAAATGAAAATCAAATAAATAAAAAACGGTAAATTAAAA---------
Salvia_xanthocheila_18   CTAAAATATTTTTTTGTTTATTGATAAAACTTATTTTTGGGTTGTACTTTTTAAAATGAAAATCAAATAAATCAAAAACGGTAAATTAAAA---------
                         501                                                                                                 
Salvia_aethiopis_1       ---ATGGGGGGCTTAATTCCTAGTAAGACTTGCTGGTTTTACAAGAGTTCCAAGTCTAAAATCCACAACAAAACTAAAATAATGAACCTTCAAGTACATA
Salvia_amarissima_2      ---GGGGGAAGCTTAATTCATAGTAAAACTTGCTGGTTTTAAAAGAGTTACGAGTCTAAAATCCACAATAAAACTAAAACAATGAACCTTCAAGTACATA
Salvia_chinensis_3       TTTTGGGGGGGCTTAATGCATAGTAAAACTTGCTGCTTTTACAAGAGTTCCAAGTCTAAAACCCACAATAAAACTAAAACAATGAACCTTCAAGTACATA
Salvia_dorrii_4          TTTTGGGGGGGCTTAATTCATAGTAAAACTTGCTGGTTTTACAAGAGTTCCAAGTCTAAAATCCACAATAAAACTAAAACAATGAACCTTCAAGTACATA
Salvia_eremostachya_5    TTT-TGGGGGGCTTAATTCATAGTAAAACTTGCTGGTTTTACAAGAGTTCCAAGTCTAAAATCCACAATAAAACTAAAACAATGAACCTTCAAGTACATA
Salvia_fruticosa_6       ---ATGGGGGGCTTAATTCATAGTAAGACTTGCTAGTTTTACAAGAGTTCCA-----------------AAAACTAAAATAATGAACCTTCAAGTACATA
Salvia_huberi_7          ---ATGGGGGGCTTAATTCATAGTAAGACTTGCTAGTTTTACAAGAGTTCCA-----------------AAAACTAAAATAATGAACCTTCAAGTACATA
Salvia_kronenburgii_8    ---ATGGGGGGCTTAATTCATAGTAAGACTTGCTAGTTTTACAAGAGTTCCA-----------------AAAACTAAAATAATGAACCTTCAAGTACATA
Salvia_limbata_9         ---ATGGGGGGCTTAATTCCTAGTAAGACTTGCTGGTTTTACAAGAGTTCCAAGTCTAAAATCCACAACAAAACTAAAATAATGAACCTTCAAGTACATA
Salvia_mohavensis_10     TTTGGGGGGGGCTTAATTCATAGTAAAACTTGCTGGTTTTACAAGAGTTCCAAGTCTAAAATCCACAATAAAACTAAAACAATGAACCTTCAAGTACATA
Salvia_officinalis_subsp ---ATGGGGGGCTTAATTCATAGTAAGACTTGCTAGTTTTACAAGAGTTCCA-----------------AAAACTAAAATAATGAACCTTCAAGTACATA
Salvia_pachyphylla_12    ----------------------------------------------------------------------------------------------------
Salvia_pratensis_13      ---ATGGGGGGCTTAATTCCTAGTAAGACTTGCTGGTTTTACAAGAGTTCCAAGTCTAAAATCCACAACAAAACTAAAATAATGAACCTTCAAGTACATA
Salvia_saccardiana_14    ---ATGGGGGGCTTAATTCCTAGTAAGACTTGCTGGTTTTACAAGAGTTCCAAGTCTAAAATCCACAACAAAACTAAAATAATGAACCTTCAAGTACATA
Salvia_sonomensis_15     ----------------------------------------------------------------------------------------------------
Salvia_spathacea_16      TTTTGGGGGGGCTTAATTCATAGTAAAACTTGCTGGTTTTACAAGAGTTCCAAGTCTAAAATCCACAATAAAACTAAAACAATGAACCTTCAAGTACATA
Salvia_verbenaca_17      ---ATGGGGGTCTTAATTCCTAGTAAGACTTGCTGGTTTTACAAGAGTTCCAAGTCTAAAATCCACAACAAAACTAAAATAATGAACCTTCAAGTACATA
Salvia_xanthocheila_18   ---ATGGGGGGCTTAATTCCTAGTAAGACTTGCTGGTTTTACAAGAGTTCCAAGTCTAAAATCCACAACAAAACTAAAATAATGAACCTTCAAGTACATA
                         601                                                                                                 
Salvia_aethiopis_1       TTTGAACAAATTTTTATTCATCGATTTGAATCTTTTCTAGAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATGGGGTCAAGA
Salvia_amarissima_2      TTTGAAGAAATTGGTATTCATCGATTTGAATCTTTCCTGCAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATAAAGTCAAGA
Salvia_chinensis_3       TTTGAAGAAATTTGTATTCATCAATTTGAATCTTTCCAACAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATAGGGTCAAGA
Salvia_dorrii_4          TTTGAACAAATATGTATTCATCGATTTGAATCTTTCCTGCAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATAAAGTCAAGA
Salvia_eremostachya_5    TTTGAACAAATTGGTATTCATCGATTTGAATCTTTCCTGCAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATAAAGTCAAGA
Salvia_fruticosa_6       TTTGAACAAATTTTTATTCATCGATTTGAATCTTTTCTAGAAAA---TGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATGGGGTCAAGA
Salvia_huberi_7          TTTGAACAAATTTTTATTCATCGATTTGAATCTTTTCTAGAAAA---TGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATGGGGTCAAGA
Salvia_kronenburgii_8    TTTGAACAAATTTTTATTCATCGATTTGAATCTTTTCTAGAAAA---TGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATGGGGTCAAGA
Salvia_limbata_9         TTTGAACAAATTTTTATTCATCGATTTGAATCTTTTCTAGAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATGGGGTCAAGA
Salvia_mohavensis_10     TTTGAACAAATATGTATTCATCGATTTGAATCTTTCCTGCAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATAAAGTCAAGA
Salvia_officinalis_subsp TTTGAACAAATTTTTATTCATCGATTTGAATCTTTTCTAGAAAA---TGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATGGGGTCAAGA
Salvia_pachyphylla_12    -------------------------------------------------------------TAGACGATTTCTTATTCATAGGCTATTATAAAGTCAAGA
Salvia_pratensis_13      TTTGAACAAATTTTTATTCATCGATTTGAATCTTTTCTAGAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATGGGGTCAAGA
Salvia_saccardiana_14    TTTGAACAAATTTTTATTCATCGATTTGAATCTTTTCTAGAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATGGGGTCAAGA
Salvia_sonomensis_15     -------------------------------------------------------------TAGACGATTTCTTATTCATAGGCTATTATAAAGTCAAGA
Salvia_spathacea_16      TTTGAACAAATATGTATTCATCGATTTGAATCTTTCCTGCAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATAAAGTCAAGA
Salvia_verbenaca_17      TTTGAACAAATTTTTATTCATCGATTTGAATCTTTTCTAGAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATGGGGTCAAGA
Salvia_xanthocheila_18   TTTGAACAAATTTTTATTCATCGATTTGAATCTTTTCTAGAAAATATTGCATTCTTAAATCTAGACGATTTCTTATTCATAGGCTATTATGGGGTCAAGA
                         701
Salvia_aethiopis_1       CAA
Salvia_amarissima_2      CAG
Salvia_chinensis_3       CAA
Salvia_dorrii_4          CAA
Salvia_eremostachya_5    CAA
Salvia_fruticosa_6       CAA
Salvia_huberi_7          CAA
Salvia_kronenburgii_8    CAA
Salvia_limbata_9         CAA
Salvia_mohavensis_10     CAA
Salvia_officinalis_subsp CAA
Salvia_pachyphylla_12    CAA
Salvia_pratensis_13      CAA
Salvia_saccardiana_14    CAA
Salvia_sonomensis_15     CAA
Salvia_spathacea_16      CAA
Salvia_verbenaca_17      CAA
Salvia_xanthocheila_18   CAA

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCCCACATACGACGAAGATT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 1068-1510 bp 0.777778 81.5
rpl32-trnL-UAG_p2 GCCCACATACGACGAAGATT ACCTGAGGTAGCAAATCCCT 50.0 50.0 58.1 58.0 1967 bp 0.055556 41.5
rpl32-trnL-UAG_p3 TCGGAAAAAGAAGAAGGCCC ACCTGAGGTAGCAAATCCCT 50.0 50.0 58.1 58.0 1936 bp 0.055556 41.2
rpl32-trnL-UAG_p4 AAAAGCCCACATACGACGAA ACCTGAGGTAGCAAATCCCT 45.0 50.0 57.8 58.0 1971 bp 0.055556 40.6
rpl32-trnL-UAG_p5 TCGGAAAAAGAAGAAGGCCC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 1455 bp 0.055556 40.5

Downloads

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