Hotspot

atpH-atpI

LSC · 13494-14462 · 969 bp

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Pi 0.0311
MarkerSeek score 73.00
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
969
variable_sites
94
pi
0.0311
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.00

Pi Curve

Alignment Viewer

                         1                                                                                                   
Salvia_aethiopis_1       GATAAATTCCTCATACGAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATTAAATTAGGACTTAATTATTCCATCAATAGGATTCACCTAGTC
Salvia_amarissima_2      GATAAATTCCTCGTACTAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATTAAATTAGGACTTAATTATTCCATCGATAGGATTCACCCAGTC
Salvia_chinensis_3       GATAAATTCCTCGTACCAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATTAAATTAGGACCTAATTATTCCATCGATAGGATTCACCCAGTC
Salvia_dorrii_4          GATAAATTCCTCGTACCAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATGAAATTAGGACTTAATTATTCCATCGATAGGATTCACCCAGTC
Salvia_eremostachya_5    GATAAATTCCTCGTACCAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATGAAATTAGGACTTAATTATTCCATCGATAGGATTCACCCAGTC
Salvia_fruticosa_6       GATAAATTCCTCATACGAACAAAAAGAAATGGTTAATGATACAACCAACCAATGAATTAAATTAGGACTTAATTATTCCATCAATAGGATTCACCCAGTC
Salvia_huberi_7          GATAAATTCCTCATACGAACAAAAAGAAATGGTTAATGATACAACCAACCAATGAATTAAATTAGGACTTAATTATTCCATCAATAGGATTCACCCAGTC
Salvia_kronenburgii_8    GATAAATTCCTCATACGAACAAAAAGAAATGGTTAATGATACAACCAACCAATGAATTAAATTAGGACTTAATTATTCCATCAATAGGATTCACCCAGTC
Salvia_limbata_9         GATAAATTCCTCATACGAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATTAAATTAGGACTTAATTATTCCATCAATAGGATTCACCTAGTC
Salvia_mohavensis_10     GATAAATTCCTCGTACCAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATGAAATTAGGACTTAATTATTCCATCGATAGGATTCACCCAGTC
Salvia_officinalis_subsp GATAAATTCCTCATACGAACAAAAAGAAATGGTTAATGATACAACCAACCAATGAATTAAATTAGGACTTAATTATTCCATCAATAGGATTCACCCAGTC
Salvia_pachyphylla_12    GATAAATTCCTCGTACCAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATGAAATTAGGACTTAATTATTCCATCGATAGGATTCACCCAGTC
Salvia_pratensis_13      GATAAATTCCTCATACGAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATTAAATTAGGACTTAATTATTCCATCAATAGGATTCACCTAGTC
Salvia_saccardiana_14    GATAAATTCCTCATACGAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATTAAATTAGGACTTAATTATTCCATCAATAGGATTCACCTAGTC
Salvia_sonomensis_15     GATAAATTCCTCGTACCAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATGAAATTAGGACTTAATTATTCCATCGATAGGATTCACCCAGTC
Salvia_spathacea_16      GATAAATTCCTCGTACCAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATGAAATTAGGACTTAATTATTCCATCGATAGGATTCACCCAGTC
Salvia_verbenaca_17      GATAAATTCCTCATACGAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATTAAATTAGGACTTAATTATTCCATCAATAGGATTCACCTAGTC
Salvia_xanthocheila_18   GATAAATTCCTCATACGAACAAAAAGAAATGGTTAATGATACAATCAACCAATGAATTAAATTAGGACTTAATTATTCCATCAATAGGATTCACCTAGTC
                         101                                                                                                 
Salvia_aethiopis_1       GAAGGAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTCCGAT--------------------------------------
Salvia_amarissima_2      GAAGTAACTAAGAACTTCGAATTTAAGTAAGAATATTATTCAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_chinensis_3       GAAGTAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTTCGATATCTCTTTTTCGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_dorrii_4          GAAGTAACTAAGAACTTCGAATTTAAGTAAGAATATTATTCAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_eremostachya_5    GAAGTAACTAAGAACTTCGAATTTAAGTAAGAATATTATTCAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_fruticosa_6       GAAGGAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_huberi_7          GAAGGAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_kronenburgii_8    GAAGGAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_limbata_9         GAAGGAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTCCGAT--------------------------------------
Salvia_mohavensis_10     GAAGTAACTAAGAACTTCGAATTTAAGTAAGAATATTATTCAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_officinalis_subsp GAAGGAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_pachyphylla_12    GAAGTAACTAAGAACTTCGAATTTAAGTAAGAATATTATTCAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_pratensis_13      GAAGGAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTCCGAT--------------------------------------
Salvia_saccardiana_14    GAAGGAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTCCGAT--------------------------------------
Salvia_sonomensis_15     GAAGTAACTAAGAACTTCGAATTTAAGTAAGAATATTATTCAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_spathacea_16      GAAGTAACTAAGAACTTCGAATTTAAGTAAGAATATTATTCAATCATCAGAACTACTTCGATATCTCTTTTTTGATTTCTATTCACAAAGTTTTTGTGAA
Salvia_verbenaca_17      GAAGGAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTCCGAT--------------------------------------
Salvia_xanthocheila_18   GAAGGAACTAAGAACTTCGAATTTAAGTAAGAATATTATTGAATCATCAGAACTACTCCGAT--------------------------------------
                         201                                                                                                 
Salvia_aethiopis_1       -------------------------------------------------------------------ATCAACCAATTTACAGCCACAATGATATGAAAG
Salvia_amarissima_2      TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGTCTTTTCTTGATTTCATCAACCAATTTACAGCCCGAACGATATGAAAG
Salvia_chinensis_3       TCCATAGACCTTTAGTTCTTCCATTTCTCGAACTGTTATTTCAATTCTTACGTCTTTTCTTGATTTCATCAGCCAATTTACAGCCACAACGATATGAAAG
Salvia_dorrii_4          TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGTATTTTCTTGATTTCATCAACCAATTTACAGCCAGAACGATATGAAAG
Salvia_eremostachya_5    TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGTATTTTCTTGATTTCATCAACCAATTTACAGCCAGAACGATATGAAAG
Salvia_fruticosa_6       TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGGCTTTTCTTGATTTCATCAACCAATTTACAGCCACAACGGTATGAAAG
Salvia_huberi_7          TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGGCTTTTCTTGATTTCATCAACCAATTTACAGCCACAACGATATGAAAG
Salvia_kronenburgii_8    TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGGCTTTTCTTGATTTCATCAACCAATTTACAGCCACAACGATATGAAAG
Salvia_limbata_9         -------------------------------------------------------------------ATCAACCAATTTACAGCCACAACGATATGAAAG
Salvia_mohavensis_10     TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGTATTTTCTTGATTTCATCAACCAATTTACAGCCAGAACGATATGAAAG
Salvia_officinalis_subsp TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGGCTTTTCTTGATTTCATCAACCAATTTACAGCCACAACGGTATGAAAG
Salvia_pachyphylla_12    TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGTATTTTCTTGATTTCATCAACCAATTTACAGCCAGAACGATATGAAAG
Salvia_pratensis_13      -------------------------------------------------------------------ATCAACCAATTTACAGCCACAACGATATGAAAG
Salvia_saccardiana_14    -------------------------------------------------------------------ATCAACCAATTTACAGCCACAACGATATGAAAG
Salvia_sonomensis_15     TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGTATTTTCTTGATTTCATCAACCAATTTACAGCCAGAACGATATGAAAG
Salvia_spathacea_16      TCCATAGACCTTTAGTTCTTCCATTTCCCGAACTGTTATTTCAATTCTTACGTATTTTCTTGATTTCATCAACCAATTTACAGCCAGAACGATATGAAAG
Salvia_verbenaca_17      -------------------------------------------------------------------ATCAACCAATTTAAAGCCACAACGATATGAAAG
Salvia_xanthocheila_18   -------------------------------------------------------------------ATCAACCAATTTACAGCCACAACGATATGAAAG
                         301                                                                                                 
Salvia_aethiopis_1       GACTTCTCTTGGAACCCCATACGCAGCGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_amarissima_2      GACTTCTATTGGAACCCCACACACAGTGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_chinensis_3       GACTTTTATTGGAACCCCACACGCAGTGGTGGGGCAAATGAAATTTTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_dorrii_4          GACTTCTATTGGAACCCCACACGCAGTGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_eremostachya_5    GACTTCTATTGGAACCCCACACGCAGTGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_fruticosa_6       GACTTCTATTGGAACCCCGTACGCAGCGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACACGTCTTTC
Salvia_huberi_7          GACTTCTATTGGAACCTCGTACGCAGCGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACACGTCTTTC
Salvia_kronenburgii_8    GACTTCTATTGGAACCCCGTACGCAGCGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACACGTCTTTC
Salvia_limbata_9         GACTTCTCTTGGAACCCCATACGCAGCGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_mohavensis_10     GACTTCTATTGGAACCCCACACGCAGTGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_officinalis_subsp GACTTCTATTGGAACCCCGTACGCAGGGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACACGTCTTTC
Salvia_pachyphylla_12    GACTTCTATTGGAACCCCACACGCAGTGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_pratensis_13      GACTTCTCTTGGAACCCCATACGCAGCGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATTTAATATCACATATACATGTCTTTC
Salvia_saccardiana_14    GACTTCTCTTGGAACCCCATACGCAGCGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATTTAATATCACATATACATGTCTTTC
Salvia_sonomensis_15     GACTTCTATTGGAACCCCACACGCAGTGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_spathacea_16      GACTTCTATTGGAACCCCACACGCAGTGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_verbenaca_17      GACTTCTCTTGGAACCCCATACGCAGCGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
Salvia_xanthocheila_18   GACTTCTCTTGGAACCCCATACGCAGCGGTGGGGCAAATGAAATATTTCTTTAGTTCATATACAACTAGCAATATCTAATATCACATATACATGTCTTTC
                         401                                                                                                 
Salvia_aethiopis_1       TTCCATAACGTAAACCATTAGATTCAATCGGATTTGGGAATCAATCCATTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAACGGAATACAATCTA
Salvia_amarissima_2      TTCCATAACGTAAACCATTAGATTCAATCGGATTTTGGAATCAATCCGTTTTTTCAGGAATCCCGTTTTGTTTATCATTATTCCAGCGGAAGACAATCTA
Salvia_chinensis_3       TTCCATAACGTAAACCATTAGATTCAATCGGATTTGGGAATCAATCCGTTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAGCGGAATACAATCTA
Salvia_dorrii_4          TTCCATAACGTAAACCATTAGATTCAATCGGATTGGGGAATCAATCCGTTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAGCGGAATACAATCTA
Salvia_eremostachya_5    TTCCATAACGTAAACCATTAGATTCAATCGGATTGGGGAATCAATCCGTTTTTTTAGGAATCCCGTTTTGTTTATCATTATTTCAGCGGAATACAATCTA
Salvia_fruticosa_6       TTCCATAACGTAAACCATTAGATTCAATCGGATTTGGGAATCAATCCATTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAACGGAATACAATCTA
Salvia_huberi_7          TTCCATAACGTAAACCATTAGATTCAATCGGATTTGGGAATCAATCCATTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAACGGAATACAATCTA
Salvia_kronenburgii_8    TTCCATAACGTAAACCATTAGATTCAATCGGATTTGGGAATCAATCCATTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAACGGAATACAATCTA
Salvia_limbata_9         TTCCATAACGTAAACCATTAGATTCAATCGGATTGGGGAATCAATCCATTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAACGGAATACAATCTA
Salvia_mohavensis_10     TTCCATAACGTAAACCATTAGATTCAATCGGATTGGGGAATCAATCCGTTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAGCGGAATACAATCTA
Salvia_officinalis_subsp TTCCATAACGTAAACCATTAGATTCAATCGGATTTGGGAATCAATCCATTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAACGGAATACAATCTA
Salvia_pachyphylla_12    TTCCATAACGTAAACCATTAGATTCAATCGGATTGGGGAATCAATCCGTTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAGCGGAATACAATCTA
Salvia_pratensis_13      TTCCATAACGTAAACCATTAGATTCAATCGGATTGGGGAATCAATCCATTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAATGGAATACAATCTA
Salvia_saccardiana_14    TTCCATAACGTAAACCATTAGATTCAATCGGATTGGGGAATCAATCCATTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAATGGAATACAATCTA
Salvia_sonomensis_15     TTCCATAACGTAAACCATTAGATTCAATCGGATTGGGGAATCAATCCGTTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAGCGGAATACAATCTA
Salvia_spathacea_16      TTCCATAACGTAAACCATTAGATTCAATCGGATTGGGGAATCAATCCGTTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAGCGGAATACAATCTA
Salvia_verbenaca_17      TTCCATAACGTAAACCATTAGATTCAATCGGATTTGGGAATCAATCCATTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAACGGAATACAATCTA
Salvia_xanthocheila_18   TTCCATAACGTAAACCATTAGATTCAATCGGATTTGGGAATCAATCCATTTTTTTAGGAATCCCGTTTTGTTTATCATTATTCCAACGGAATACAATCTA
                         501                                                                                                 
Salvia_aethiopis_1       AATGGGCAATTAAAATTGATTAGCGCTAATTATTGCACAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTTTGATTTTGAAATATTTTGGTCA
Salvia_amarissima_2      AATAGGCAAATAAAATTGATTAGCGCGAATTATTGCATAGAAAAAGATTATTTTATTAATCTAAGTTCATGCAATTTATTATTTTTCAATATTTTGTTCA
Salvia_chinensis_3       AATGGGCAAATTAAATTGATTAGCGAGAATTAGTGCATAGAAAAAGATTATTTTATTGATCTAAGTTCAGGCAATTTATTATTTTTCAATATTTTGGTCA
Salvia_dorrii_4          AATGGGCAATTTAAATTGATTAGCGCGAATTATTGCATAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTCTTATTTTTCAATATTTTGGTCA
Salvia_eremostachya_5    AATGGGCAATTTAAATTGATTAGCGCGAATTATTGCATAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTATTATTTTTCAATATTTTGGTCA
Salvia_fruticosa_6       AATGGGCAATTTAAATTGATTAGCGCGAATTATTGCACAGAAAAAGATTATTTTATTGATCTAAGTTCATGCCATTTTTTATTTTGAAATATTTTGGTCA
Salvia_huberi_7          AATGGGCAATTTAAATTGATTAGCGCGAATTATTGCACAGAAAAAGATTATTTTATTGATCTAAGTTCATGCCATTTTTTATTTTGAAATATTTTGGTCA
Salvia_kronenburgii_8    AATGGGCAATTTAAATTGATTAGCGCGAATTATTGCACAGAAAAAGATTATTTTATTGATCTAAGTTCATGCCA-TTTTTATTTTGAAATATTTTGGTCA
Salvia_limbata_9         AATGGGCAATTTAAATTGATTAGCGCTAATTATTGCACAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTTTTATTTTGAAATATTTTGGTCA
Salvia_mohavensis_10     AATGGGCAATTTAAATTGATTAGCGCGAATTATTGCATAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTATTATTTTTCAATATTTTGGTCA
Salvia_officinalis_subsp AATGGGCAATTTAAATTGATTAGCGCGAATTATTGCACAGAAAAAGATTATTTTATTGATCTAAGTTCATGCCATTTTTTATTTTGAAATATTTTGGTCA
Salvia_pachyphylla_12    AATGGGCAATTTAAATTGATTAGCGCGAATTATTGCATAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTATTATTTTTCAATATTTTGGTCA
Salvia_pratensis_13      AATGGGCAATTTAAATTGATTAGCGCTAATTATTGCACAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTTTGATTTTGAAATATTTTGGTCA
Salvia_saccardiana_14    AATGGGCAATTTAAATTGATTAGCGCTAATTATTGCACAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTTTGATTTTGAAATATTTTGGTCA
Salvia_sonomensis_15     AATGGGCAATTTAAATTGATTAGCGCGAATTATTGCATAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTATTATTTTTCAATATTTTGGTCA
Salvia_spathacea_16      AATGGGCAATTTAAATTGATTAGCGCGAATTATTGCATAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTCTTATTTTTCAATATTTTGGTCA
Salvia_verbenaca_17      AATGGGCAATTTAAATTGATTAGCGCTAATTATTGCACAGAAAAATATTATTTTATTGATCTAAGTTCATGCAATTTTTGATTTTGAAATATTTTGGTCA
Salvia_xanthocheila_18   AATGGGCAATTTAAATTGATTAGCGCTAATTATTGCACAGAAAAAGATTATTTTATTGATCTAAGTTCATGCAATTTTTGATTTTGAAATATTTTGGTCA
                         601                                                                                                 
Salvia_aethiopis_1       ATTGTTGAATAAAATCTACTCTAAAATTGTAAAATAAAGGAGAATCCTTTCTCCTGTTTTTTATTTAATCACACGCTGTAAAGATATATCTTATTCAAAT
Salvia_amarissima_2      ATTGTTGAATAAAATCAACTCTAAAATTGTAA----------AATCCTTTGTCCTGTTTTTTATTTAATTACACGCCCTAAACATATATCTTATTCAAAT
Salvia_chinensis_3       ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGGAGAATCCTTTATCCTGTTTTTCAGTTAATCACACACCCTAAACATATATCTTATTCAAAT
Salvia_dorrii_4          ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGGAGAATCCTTTGTCCTGTTTTTTATTTAATCACACGCCCTAAACATATATCTTATTCAAAT
Salvia_eremostachya_5    ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGGAAAATCCTTTGTCCTGTTTTTTATTTAATCACACGCCCTAAACATATATCTTATTCAAAT
Salvia_fruticosa_6       ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGGAGAATCTTTTCTCCTGTTTTTTATTTAATCACACGCCGTAAAGATATATCTTATTCAAAT
Salvia_huberi_7          ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGG------TTTTCTCCTGTTTTTTATTTAATCACACGCCGTAAAGATATATCTTATTCAAAT
Salvia_kronenburgii_8    ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGGAGAATCTTTTCTCCTGTTTTTTATTTAATCACACGCCGTAAAGATATATCTTATTCAAAT
Salvia_limbata_9         ATTGTTGAATAAAATCTACTCTAAAATTGTAAAATAAAGGAGAATCCTTTCTCCTGTTTTTTATTTAATCACACGCCGTAAAGATATATCTTATTCAAAT
Salvia_mohavensis_10     ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGGAGAATCCTTTGTCCTGTTTTTTATTTAATCACACGCCCTAAACATATATCTTATTCAAAT
Salvia_officinalis_subsp ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGGAGAATCTTTTCTCCTGTTTTTTATTTAATCACACGCCGTAAAGATATATCTTATTCAAAT
Salvia_pachyphylla_12    ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGGAGAATCCTTTGTCCTGTTTTTTATTTAATCACACGCCCTAAACATATATCTTATTCAAAT
Salvia_pratensis_13      ATTGTTGAATAAAATCTACTCTAAAATTGTAAAATAAAGGAGAATCCTTTCTCCTGTTTTTTATTTAATCACACGCCGTAAAGATATATCTTATTCAAAT
Salvia_saccardiana_14    ATTGTTGAATAAAATCTACTCTAAAATTGTAAAATAAAGGAGAATCCTTTCTCCTGTTTTTTATTTAATCACACGCCGTAAAGATATATCTTATTCAAAT
Salvia_sonomensis_15     ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGGAGAATCCTTTGTCCTGTTTTTTATTTAATCACACGCCCTAAACATATATCTTATTCAAAT
Salvia_spathacea_16      ATTGTTGAATAAAATCAACTCTAAAATTGTAAAATAAAGGAGAATCCTTTGTCCTGTTTTTTATTTAATCACACGCCCTAAACATATATCTTATTCAAAT
Salvia_verbenaca_17      ATTGTTGAATAAAATCTACTCTAAAATTGTAAAATAAAGGAGAATCCTTTCTCCTGTTTTTTATTTAATCACACGCCGTAAAGATATATCTTATTCAAAT
Salvia_xanthocheila_18   ATTGTTGAATAAAATCTACTCTAAAATTGTAAAATAAAGGAGAATCCTTTCTCCTGTTTTTTATTTAATCACACGCCGTAAAGATATATCTTATTCAAAT
                         701                                                                                                 
Salvia_aethiopis_1       TATGATTTGAATAAGAAGGTTGACTCGCTAATAGAATAGAAAGTGAATATTCCTCGAAGAAGGGTAGGATATTAAGTGATTATTTTAGGAAAAAGATTTT
Salvia_amarissima_2      TATGATTTGAATAAGAAGATTGACTCGCCAATAGAATAGAAAGTGAATATTCCTCGAAAAAGGGTAAGATATTAAGTGATTTTTTTAGGAAAAATATTTT
Salvia_chinensis_3       TATGATTTGAATAAGAAGATTGACTCGCCAATAGAATAGAAAGTGAATATTCCTCGAAAAAGGGTAAGATATTAAGTGATTATTTTAGGAAAAAGATTTT
Salvia_dorrii_4          TATGATTTGAATAAGAAGATTGACTCGCCAATAGAATAGAAAGTGAATATTCCTCGAAAAAAGGTAAGATATTAAGTGATTTTTTTAGGAAATTTTTTTT
Salvia_eremostachya_5    TATGATTTGAATAAGAAGATTGACTCGCCAATAGAATAGAAAGTGAATATTCCTCGAAAAAAGGTAAGATATTAAGTGATTTTTTTAGGAAAAATATTTT
Salvia_fruticosa_6       TATGATTTGAATAAGAAGGTTGACTCGCTAATAGAATAGAAAGTGAATATTCCTCGAAGAAGGGTAGAATATTAAGTGATTATTTTAGGAAAAAGATTTT
Salvia_huberi_7          TATGATTTGAATAAGAAGGTTGACTCACTAATAGAATAGAAAGTGAATATTCCTCGAAGAAGGGTAGAATATTAAGTGATTATTTTAGGAAAAAGATTTT
Salvia_kronenburgii_8    TATGATTTGAATAAGAAGGTTGACTCACTAATAGAATAGAAAGTGAATATTCCTCGAAGAAGGGTAGAATATTAAGTGATTATTTTAGGAAAAAGATTTT
Salvia_limbata_9         TATGATTTGAATAAGAAGGTTGACTCGCTAATAGAATAGAAAGTGAATATTCCTCGAAGAAGGGTAGGATATTAAGTGATTATTTTAGGAAAAAGATTTT
Salvia_mohavensis_10     TATGATTTGAATAAGAAGATTGACTCGCCAATAGAATAGAAAGTGAATATTCCTCGAAAAAAGGTAAGATATTAAGTGATTTTTTTAGGAAAAATTTTTT
Salvia_officinalis_subsp TATGATTTGAATAAGAAGGTTGACTCGCTAATAGAATAGAAAGTGAATATTCCTCGAAGAAGGGTAGAATATTAAGTGATTATTTTAGGAAAAAGATTTT
Salvia_pachyphylla_12    TATGATTTGAATAAGAAGATTGACTCGCCAATAGAATAGAAAGTGAATATTCCTCGAAAAAAGGTAAGATATTAAGTGATTTTTTTAGGAAAAATTTTTT
Salvia_pratensis_13      TATGATTTGAATAAGAAGGTTGACTCGCTAATAGAATAGAAAGTGAATATTCCTCGAAGAAGGGTAGGATATTAAGTGATTCTTTTAGGAAAAAGATTTT
Salvia_saccardiana_14    TATGATTTGAATAAGAAGGTTGACTCGCTAATAGAATAGAAAGTGAATATTCCTCGAAGAAGGGTAGGATATTAAGTGATTCTTTTAGGAAAAAGATTTT
Salvia_sonomensis_15     TATGATTTGAATAAGAAGATTGACTCGCCAATAGAATAGAAAGTGAATATTCCTCGAAAAAAGGTAAGATATTAAGTGATTTTTTTAGGAAAAATTTTTT
Salvia_spathacea_16      TATGATTTGAATAAGAAGATTGACTCGCCAATAGAATAGAAAGTGAATATTCCTCGAAAAAAGGTAAGATATTAAGTGATTTTTTTAGGAAATTTTTTTT
Salvia_verbenaca_17      TATGATTTGAATAAGAAGGTTGACTCGCTAATAGAATAGAAAGTGAATATTCCTCAAAGAAGGATAGGATATTAAGTAATTATTTTAGGAAAAAGATTTT
Salvia_xanthocheila_18   TATGATTTGAATAAGAAGGTTGACTCGCTAATAGAATAGAAAGTTAATATTCCTCGAAGAAGGGTAGGATATTAAGTGATTATTTTAGGAAAAAGATTTT
                         801                                                                                                 
Salvia_aethiopis_1       TTAGATCTCTTTCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTGATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_amarissima_2      TTAGATCTCTTGCC-TTTTTTTTACAACTCTCTAATATCATACTTTTTTATTTTTT--TTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_chinensis_3       TTAGATCTCTTGCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTTATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_dorrii_4          TTAGATCTCTTGCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTTATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_eremostachya_5    TTAGATCTCTTGCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTTATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_fruticosa_6       TTAGGTCTCTTTCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTGATTTTTTTGTTCCTATTTCTTTCTAGCATAAATAGAGTCTTGTATATTTCT
Salvia_huberi_7          TTAGGTCTCTTTCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTGATTTTTTTGTTCCTATTTCTTTCTAGCATAAATAGAGTATTGTATATTTCT
Salvia_kronenburgii_8    TTAGGTCTCTTTCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTGATTTTTTTGTTCCTATTTCTTTCTAGCATAAATAGAGTCTTGTATATTTCT
Salvia_limbata_9         TTAGATCTCTTTCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTGATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_mohavensis_10     TTAGATCTCTTGCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTTATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_officinalis_subsp TTAGGTCTCTTTCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTGATTTTTTTGTTCCTATTTCTTTCTAGCATAAATAGAGTCTTGTATATTTCT
Salvia_pachyphylla_12    TTAGATCTCTTGCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTTATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_pratensis_13      TTAGATCTCTTTCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTGATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_saccardiana_14    TTAGATCTCTTTCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTGATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_sonomensis_15     TTAGATCTCTTGCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTTATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_spathacea_16      TTAGATCTCTTGCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTTATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_verbenaca_17      TTAGATCTCTTTCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTGATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
Salvia_xanthocheila_18   TTAGATCTCTTTCCTTTTTTTTTACAACTCTCTAATATCGTAATTTTTGATTTTTTTGTTCCTATTTCTTTCTAGCATATATAGAGTCTTGTATATTTCT
                         901                                                                  
Salvia_aethiopis_1       A-----TTCTTTAACTAAATCATCTTGAGTCACATATGCGTTGCTTTGCACTAAGCAAAAAAGACTATT
Salvia_amarissima_2      A-----TTCTTTAAGTAAATCATCTTGAGCCATATATGCGTTGCTTTGCACTAAGCGAAAAAGACTATT
Salvia_chinensis_3       ATTCTTTTCTTTAAGTAAATAATATTGAGCCATATATGCGTTGCTTTGCACTAAGCGAAAAAGACTATT
Salvia_dorrii_4          A-----TTCTTTAAGTAAATCATCTTGAGCCATATATGCGTTGCTTTGCACTAAGCGAAAAAGACTATT
Salvia_eremostachya_5    A-----TTCTTTAAGTAAATCATCTTGAGCCATATATGCGTTGCTTTGCACTAAGCGAAAAAGACTATT
Salvia_fruticosa_6       A-----TTCTTTAACTAAATCATCTTGAGTCACATATGCGTTGCTTTGCACTAAGCAAAAAAGACTATT
Salvia_huberi_7          A-----TTCTTTAACTAAATCATCTTGAGTCACATATGCGTTGCTTTGCACTAAGCAAAAAAGACTATT
Salvia_kronenburgii_8    A-----TTCTTTAACTAAATCATCTTGAGTCACATATGCGTTGCTTTGCACTAAGCAAAAAAGACTATT
Salvia_limbata_9         A-----TTCTTTAACTAAATCATCTTGAGTCACATATGCGTTGCTTTGCACTAAGCAAAAAAGACTATT
Salvia_mohavensis_10     A-----TTCTTTAAGTAAATCATCTTGAGCCATATATGCGTTGCTTTGCACTAAGCGAAAAAGACTATT
Salvia_officinalis_subsp A-----TTCTTTAACTAAATCATCTTGAGTCACATATGCGTTGCTTTGCACTAAGCAAAAAAGACTATT
Salvia_pachyphylla_12    A-----TTCTTTAAGTAAATCATCTTGAGCCATATATGCGTTGCTTTGCACTAAGCGAAAAAGACTATT
Salvia_pratensis_13      A-----TTCTTTAACTAAATCATCTTGAGTCACATATGCGTTGCTTTGCACTAAGCAAAAAAGACTATT
Salvia_saccardiana_14    A-----TTCTTTAACTAAATCATCTTGAGTCACATATGCGTTGCTTTGCACTAAGCAAAAAAGACTATT
Salvia_sonomensis_15     A-----TTCTTTAAGTAAATCATCTTGAGCCATATATGCGTTGCTTTGCACTAAGCGAAAAAGACTATT
Salvia_spathacea_16      A-----TTCTTTAAGTAAATCATCTTGAGCCATATATGCGTTGCTTTGCACTAAGCGAAAAAGACTATT
Salvia_verbenaca_17      A-----TTCTTTAACTAAATCATCTTGAGTCACATATGCGTTGCTTTGCACTAAGCAAAAAAGACTATT
Salvia_xanthocheila_18   A-----TTCTTTAACTAAATCATCTTGAGTCACATATGCGTTGCTTTGCACTAAGCAAAAAAGACTATT

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACGGAAGCAGCAGAAATCA TCCCTGTCATGTTCCTTGGA 45.0 50.0 58.1 58.3 1044-1169 bp 1.0 87.9
atpH-atpI_p2 AGCCAATCCAGCAGCAATAA TCCCTGTCATGTTCCTTGGA 45.0 50.0 57.9 58.3 1062-1187 bp 1.0 87.4
atpH-atpI_p3 GCCAATCCAGCAGCAATAAC TCCCTGTCATGTTCCTTGGA 50.0 50.0 57.8 58.3 1061-1186 bp 1.0 86.9
atpH-atpI_p4 TCCAGCAGCAATAACGGAAG TCCCTGTCATGTTCCTTGGA 50.0 50.0 58.3 58.3 1056-1181 bp 1.0 86.5
atpH-atpI_p5 AACGGAAGCAGCAGAAATCA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.1 58.5 996-1121 bp 1.0 86.1

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