Pi
0.0383
MarkerSeek score
62.90
Primer available
yes
Label
psbK-psbI
Feature Metadata
Pi Curve
Alignment Viewer
1
Salvia_aethiopis_1 GATCCTGAATAATAATATCCTAGAAAATGCGTGATTTCCTCGAGAAAAAATTATAAAAATTG------AAAAAATAAGATAAGTTTTAGAGTATGAACCC
Salvia_amarissima_2 GATCCGGAATAATAATATCCTAGAAAATGCATGATTTCCTCGAGAAAAAATTCTAACAATTG------ACAAAATCAGATAAATTTTAGAGTATGAACCT
Salvia_chinensis_3 GATCCTGAATAATAATATCCTAGAAAATACATGATTTCCTCGAGAAAAAATTATAACAATTGACAAAAACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_dorrii_4 GATCCTGAATAATAATATCCTAGAAAATGCATGATTTCCTCGAGAAAAAATTCTAAGAATTG------ACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_eremostachya_5 GATCCTGAATAATAATATCCTAGAAAATGCATGATTTCCTCGAGAAAAAATTCTAAGAATTG------ACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_fruticosa_6 GATCCTGAATAATAATATCCTAGAAAATGCGTGATTTCCTCGAGAAAAAATTATAACAATTG------ACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_huberi_7 GATCCTGAATAATAATATCCTAGAAAATGCGTGATTTCCTCGAGAAAAAATTATAACAATTG------ACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_kronenburgii_8 GATCCTGAATAATAATATCCTAGAAAATGCGTGATTTCCTCGAGAAAAAATTATAACAATTG------ACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_limbata_9 GATCCTGAATAATAATATCCTAGAAAATGCGTGATTTCCTCGAGAAAAAATTATAAAAATTG------AAAAAATAAGATAAGTTTTAGAGTATGAACCC
Salvia_mohavensis_10 GATCCTGAATAATAATATCCTAGAAAATGCATGATTTCCTCGAGAAAAAATTCTAAGAATTG------ACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_officinalis_subsp GATCCTGAATAATAATATCCTAGAAAATGCGTGATTTCCTCGAGAAAAAATTATAACAATTG------ACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_pachyphylla_12 GATCCTGAATAATAATATCCTAGAAAATGCATGATTTCCTCGAGAAAAAATTCTAAGAATTG------ACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_pratensis_13 GATCCTGAATAATAATATCCTAGAAAATGCGTGATTTCCTCGAGAAAAAATTATAAAAATTG------ACAAAATAAGATAAGTTTTAGAGTATGAACCC
Salvia_saccardiana_14 GATCCTGAATAATAATATCCTAGAAAATGCGTGATTTCCTCGAGAAAAAATTATAAAAATTG------ACAAAATAAGATAAGTTTTAGAGTATGAACCC
Salvia_sonomensis_15 GATCCTGAATAATAATATCCTAGAAAATGCATGATTTCCTCGAGAAAAAATTCTAAGAATTG------ACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_spathacea_16 GATCCTGAATAATAATATCCTAGAAAATGCATGATTTCCTCGAGAAAAAATTCTAAGAATTG------ACAAAATCAGATAAGTTTTAGAGTATGAACCC
Salvia_verbenaca_17 GATCCTGAATAATAATATCCTAGAAAATGCGTGATTTCCTCGAGAAAAAATTATAAAAATTG------ACAAAATAAGATAAGTTTTAGAGTATGAACCC
Salvia_xanthocheila_18 GATCCTGAATAATAATATCCTAGAAAATGCGTGATTTCCTCGAGAAAAAATTATAAAAATTG------AAAAAATAAGATAAGTTTTAGAGTATGAACCC
101
Salvia_aethiopis_1 TCGATTCACACATTGAAATTCGTGGATAGTCGACATAAATTAAGCTTACCCCTATTTCCTCGTTTTTGAGCCTTTTCCAACCATCCAGTCAAAGACCCTA
Salvia_amarissima_2 TCGATTCACACATTG--ATTCGTGAATAGTTAACATAAACCGAACTTACCCCCATTTCCTCGTTTTTGAGCCTTTTCCAGTCATCCAGTCAAAGACCCTA
Salvia_chinensis_3 TCGATTCATACATTGAAATTCGTGAATAGTCGGCATAAATCAGGCTTACCCCCATTTCCTCGTTTTTGAGCCTTTTCCAGTCATCCAGTCAAAGACCCTA
Salvia_dorrii_4 TCGATTCACACATTGAAATTCGTGAATAGTCAACATAAATCGGGCTTACCCCCATTTCCTCGTTTTTGAGCCTTTTCCAGTCATCCAGTCAAAGACCCTA
Salvia_eremostachya_5 TCGATTCACACATTGAAATTCGTGAATAGTCAACATAAATCGGGCTTACCCCCATTTCCTCGTTTTTGAGCCTTTTCCAGTCATCCAGTCAAAGACCCTA
Salvia_fruticosa_6 TCGATTCACACATTGAAATTCGTGGATAGTCGACATAAATCAGGCTTACCCCTATTTCCTCGTTTTTGAGCCTTTTCCAGCCATCCAGTCAAAGACCCTA
Salvia_huberi_7 TCGATTCACACATTGAAATTCGTGGATAGTCGACATAAATCAGGCTTACCCCTATTTCCTCGTTTTTGAGCCTTTTCCAGCCATCCAGTCAAAGACCCTA
Salvia_kronenburgii_8 TCGATTCACACATTGAAATTCGTGGATAGTCGACATAAATCAGGCTTACCCCTATTTCCTCGTTTTTGAGCCTTTTCCAGCCATCCAGTCAAAGACCCTA
Salvia_limbata_9 TCGATTCACACATTGAAATTCGTGGATAGTCGACATAAATTAAGCTTACCCCTATTTCCTCGTTTTTGAGCCTTTTCCAACCATCCAGTCAAAGACCCTA
Salvia_mohavensis_10 TCGATTCACACATTGAAATTCGTGAATAGTCAACATAAATCGGGCTTACCCCCATTTCCTCGTTTTTGAGCCTTTTCCAGTCATCCAGTCAAAGACCCTA
Salvia_officinalis_subsp TCGATTCACACATTGAAATTCGTGGATAGTCGACATAAATCAGGCTTACCCCTATTTCCTCGTTTTTGAGCCTTTTCCAGCCATCCAGTCAAAGACCCTA
Salvia_pachyphylla_12 TCGATTCACACATTGAAATTCGTGAATAGTCAACATAAATCGGGCTTACCCCCATTTCCTCGTTTTTGAGCCTTTTCCAGTCATCCAGTCAAAGACCCTA
Salvia_pratensis_13 TCGATTCACACATTGAAATTCGCGGATAGTCGACATAAATTAAGCTTACCCCTATTTCCTCGTTTTTGAGCCTTTTCCAACCATCCAGTCAAAGACCCTA
Salvia_saccardiana_14 TCGATTCACACATTGAAATTCGCGGATAGTCGACATAAATTAAGCTTACCCCTATTTCCTCGTTTTTGAGCCTTTTCCAACCATCCAGTCAAAGACCCTA
Salvia_sonomensis_15 TCGATTCACACATTGAAATTCGTGAATAGTCAACATAAATCGGGCTTACCCCCATTTCCTCGTTTTTGAGCCTTTTCCAGTCATCCAGTCAAAGACCCTA
Salvia_spathacea_16 TCGATTCACACATTGAAATTCGTGAATAGTCAACATAAATCGGGCTTACCCCCATTTCCTCGTTTTTGAGCCTTTTCCAGTCATCCAGTCAAAGACCCTA
Salvia_verbenaca_17 TCGATTCACACATTGAAATTCGTGGATAGTCGACATAAATTAAGCTTACCCCTATTTCCTCGTTTTTAAGCCTTTTCCAACCATCCAGTCAAAGACCCTA
Salvia_xanthocheila_18 TCGATTCACACATTGAAATTCGTGGATAGTCGACATAAATTAAGCTTACCCCTATTTCCTCGTTTTTGAGCCTTTTCCAACCATCCAGTCAAAGACCCTA
201
Salvia_aethiopis_1 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGAATCTAAGCGCAAATAAAATTTTTGTTAATGAAAAACAAATAAATTTGTGATAATACATTTCTTGA
Salvia_amarissima_2 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATATATGCGCAAATTCTATTTTTGTTAATGAAAAAAAAATGAATTTGTGACAATACATTTCTTGA
Salvia_chinensis_3 ACCCTATTAGGGTCTACCACAATATCTAAATTTGGATATAAGCGCAAATTTCATTTTTGTTAATGAAAAACAAATGAATTTGTGACAATACATTTCTTGA
Salvia_dorrii_4 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATATAGGCGCAAATTGCATTTTTGTTAATGAAAAACAAATGAATTTGTGACAATACATTTCTTGA
Salvia_eremostachya_5 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATATAGGCGCAAATTTCATTTTTGTTAATGAAAAAAAAATGAATTTGTGACAATACATTTCTTGA
Salvia_fruticosa_6 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATCTAAGCGCAAATAAAATTTTTGTTAATGAAAAACAAATAAATTTGTGACAATACATTTCTTGA
Salvia_huberi_7 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATCTAAGCGCAAATAAAATTTTTGTTAATGAAAAACAAATAAATTTGTGACAATACATTTCTTGA
Salvia_kronenburgii_8 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATCTAAGCGCAAATAAAATTTTTGTTAATGAAAAACAAATAAATTTGTGACAATACATTTCTTGA
Salvia_limbata_9 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATCTAAGCGCAAATAAAATTTTTGTTAATGAAAAACAAATAAATTTGTGATAATACATTTCTTGA
Salvia_mohavensis_10 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATATAGGCGCAAATTGCATTTTTGTTAATGAAAAACAAATGAATTTGTGACAATACATTTCTTGA
Salvia_officinalis_subsp ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATCTAAGCGCAAATAAAATTTTTGTTAATGAAAAACAAATAAATTTGTGACAATACATTTCTTGA
Salvia_pachyphylla_12 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATATAGGCGCAAATTGCATTTTTGTTAATGAAAAACAAATGAATTTGTGACAATACATTTCTTGA
Salvia_pratensis_13 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATCTAAGCGCAAATAAAATTTTTGTTAATGAAAAACAAATAAATTTGTGATAATACATTTCTTGA
Salvia_saccardiana_14 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATCTAAGCGCAAATAAAATTTTTGTTAATGAAAAACAAATAAATTTGTGATAATACATTTCTTGA
Salvia_sonomensis_15 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATATAGGCGCAAATTGCATTTTTGTTAATGAAAAACAAATGAATTTGTGACAATACATTTCTTGA
Salvia_spathacea_16 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATATAGGCGCAAATTGCATTTTTGTTAATGAAAAACAAATGAATTTGTGACAATACATTTCTTGA
Salvia_verbenaca_17 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATCTAAGCGCAAATAAAATTTTTGTTAATGAAAAACAAATAAATTTGTGATAATACATTTCTTGA
Salvia_xanthocheila_18 ACCCTATTAGGGTCTCCCACAATATCTAAATTTGGATCTAAGCGCAAATAAAATTTTTGTTAATGAAAAACAAATAAATTTGTGATAATACATTTCTTGA
301
Salvia_aethiopis_1 AAAAACCTCATTTCTTGGTGTTAAAATAGGATATGTGGTAGAAAAATGGAAGATCTATTCTC-TTTTTTCTAAAAAAAAATCTTGGAGATTGTGTA
Salvia_amarissima_2 AAAAACCTCATTCCTTGGTATCAAAATAGGATATGTGGTAGAAAAATAGAAGATCTATTCTCTTTTTTTCTAAAAAAAAATCTTGGAGATTGTGTA
Salvia_chinensis_3 AAAAACCTCATTTCTTGGTATCAAAATAGGATATGTGGTAAAAAAATGGAAGATCTATTCTCTTTTTTTCGAAAAAATCATCTTGGAGATTGTGTA
Salvia_dorrii_4 AAAAACCTCATTTCTTGGTATCAAAATAGGATATGTGGTAGAAAAATAGAAGATCTATTCTCTTTTTTTCTAAAAAATAATCTTGGAGATTGTGTA
Salvia_eremostachya_5 AAAAACCTCATTTCTTGGTATCAAAATAGGATATGTGGTAGAAAAATAGAAGATCTATTCTCTTTTTTTCTAAAAAATAATCTTGGAGATTGTGTA
Salvia_fruticosa_6 AAAAACCTCATTTCTTGGTGTCAAAATAGGATATGTGGTAGAAAAATGGAAGATCTATTCTC-TTTTTTCTAAAAAATAATCTTGGAGATTGTGTA
Salvia_huberi_7 AAAAACCTCATTTCTTGGTGTCAAAATAGGATATGTGGTAGAAAAATGGAAGATCTATTCTCTTTTTTTCTAAAAAATAATCTTGGAGATTGTGTA
Salvia_kronenburgii_8 AAAAACCTCATTTCTTGGTGTCAAAATAGGATATGTGGTAGAAAAATGGAAGATCTATTCTCTTTTTTTCTAAAAAATAATCTTGGAGATTGTGTA
Salvia_limbata_9 AAAAACCTCATTTCTTGGTGTTAAAATAGGATATGTGGTAGAAAAATGGAAGATCTATTCTC-TTTTTTCTAAAAAAAAATCTTGGAGATTGTGTA
Salvia_mohavensis_10 AAAAACCTCATTTCTTGGTATCAAAATAGGAGATGTGGTAGAAAAATAGAAGATCTATTCTCTTTTTTTCTAAAAAATAATCTTGGAGATTGTGTA
Salvia_officinalis_subsp AAAAACCTCATTTCTTGGTGTCAAAATAGGATATGTGGTAGAAAAATGGAAGATCTATTCTCTTTTTTTCTAAAAAATAATCTTGGAGATTGTGTA
Salvia_pachyphylla_12 AAAAACCTCATTTCTTGGTATCAAAATAGGATATGTGGTAGAAAAATAGAAGATCTATTCTCTTTTTTTCTAAAAAATAATCTTGGAGATTGTGTA
Salvia_pratensis_13 AAAAACCTCATTTCTTGGTGTTAAAATAGGATATGTGGTAGAAAAATGGAAGATCTATTCTC-TTTTTTCT-AAAAAAAATCTTGGAGATTGTGTA
Salvia_saccardiana_14 AAAAACCTCATTTCTTGGTGTTAAAATAGGATATGTGGTAGAAAAATGGAAGATCTATTCTC-TTTTTTCT-AAAAAAAATCTTGGAGATTGTGTA
Salvia_sonomensis_15 AAAAACCTCATTTCTTGGTATCAAAATAGGATATGTGGTAGAAAAATAGAAGATCTATTCTCTTTTTTTCTAAAAAATAATCTTGGAGATTGTGTA
Salvia_spathacea_16 AAAAACCTCATTTCTTGGTATCAAAATAGGATATGTGGTAGAAAAATAGAAGATCTATTCTCTTTTTTTCTAAAAAATAATCTTGGAGATTGTGTA
Salvia_verbenaca_17 AAAAACCTCATTTCTTGGTGTTAAAATAGGATATGTGGTAGAAAAATGGAAGATCTATTCTC-TTTTTTTCTAAAAAAAATCTTGGAGATTGTGTA
Salvia_xanthocheila_18 AAAAACCTCATTTCTTGGTGTTAAAATAGGATATGTGGTAGAAAAATGGAAGATCTATTCTC-TTTTTTCTAAAAAAAAATCTTGGAGATTGTGTA
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| psbK-psbI_p1 | TAGCTTTTGTTTGGCAGGCT | ACGAAGAGTTTCAGAGTAAGCA | 45.0 | 40.9 | 58.3 | 57.4 | 454-472 bp | 1.0 | 66.5 |
| psbK-psbI_p2 | GCAGGCTGCTGTAAGTTTTC | ACGAAGAGTTTCAGAGTAAGCA | 50.0 | 40.9 | 57.7 | 57.4 | 441-459 bp | 1.0 | 66.4 |
| psbK-psbI_p3 | AGGCTGCTGTAAGTTTTCGA | ACGAAGAGTTTCAGAGTAAGCA | 45.0 | 40.9 | 57.5 | 57.4 | 439-457 bp | 1.0 | 64.8 |
| psbK-psbI_p4 | TTAGCTTTTGTTTGGCAGGC | ACGAAGAGTTTCAGAGTAAGCA | 45.0 | 40.9 | 57.5 | 57.4 | 455-473 bp | 1.0 | 64.6 |
| psbK-psbI_p5 | CAGGCTGCTGTAAGTTTTCG | ACGAAGAGTTTCAGAGTAAGCA | 50.0 | 40.9 | 57.4 | 57.4 | 440-458 bp | 1.0 | 64.3 |