Hotspot

trnK-UUU-rps16

LSC · 4267-4835 · 569 bp

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Pi 0.0325
MarkerSeek score 71.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
569
variable_sites
64
pi
0.0325
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.30

Pi Curve

Alignment Viewer

                         1                                                                                                   
Salvia_aethiopis_1       CGATAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGATATATAGACATGAATACAATCAGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_amarissima_2      CGAGAAAATATCGATTAAACAAAACTTCAGCTCAACAAGGGGTATATAGACATGGATACAATCCGAACCAAAAGCAATTAAATTGAATTAAAACAAAGAG
Salvia_chinensis_3       CGAGAAGATATCGATTAAACAAAACTTCAACTCAACAAGGGGTATATAGACATGGATACAATCAGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_dorrii_4          CGAGAAAATTTCTATTAAACAAAACTTCAACTCAACAAGGGGTATATAGACATGGATACAATCCGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_eremostachya_5    CGAGAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGGTATATAGACATGGATACAATCCGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_fruticosa_6       CGATAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGATATATAGACATGAATACAATCAGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_huberi_7          CGATAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGATATATAGACATGAATACAATCAGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_kronenburgii_8    CGATAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGATATATAGACATGAATACAATCAGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_limbata_9         CAATAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGATATATAGACATGAATACAATCAAAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_mohavensis_10     CGAGAAAATTTCGATTAAACAAAACTTCAACTCAACAAGGGGTATATAGACATGGATACAATCCGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_officinalis_subsp CGATAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGATATATAGACATGAATACAATCAGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_pachyphylla_12    CGAGAAAATTTCGATTAAACAAAACTTCAACTCAACAAGGGGTATATAGACATGGATACAATCCGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_pratensis_13      CGATAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGATATATAGACATGAATACAATCAGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_saccardiana_14    CGATAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGATATATAGACATGAATACAATCAGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_sonomensis_15     CGAGAAAATTTCGATTAAACAAAACTTCAACTCAACAAGGGGTATATAGACATGGATACAATCCGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_spathacea_16      CGAGAAAATTTCTATTAAACAAAACTTCAACTCAACAAGGGGTATATAGACATGGATACAATCCGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_verbenaca_17      CGATAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGATATATAGACATGAATACAATCAGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
Salvia_xanthocheila_18   CGATAAAATATCGATTAAACAAAACTTCAACTCAACAAGGGATATATAGACATGAATACAATCAGAACCAAAAGCAATTTAATTGAATTCAAACAAAGAG
                         101                                                                                                 
Salvia_aethiopis_1       ATTTTACGAGCTAATCAAGCCGTTGAACTAACAAATCTAAAAAAAAAAAAAGATTTTCTAATGGATTCGAAACAT-AAAATGAATTGATTAGATCAAAAT
Salvia_amarissima_2      ATTTTTAGAGCTAATCAAACCGTTGAACTAACAAATCT----AAAAAAAAAGATTTGCTAATCGATTCGAAACATAAAAATAAATTGATTAGATGAAAAT
Salvia_chinensis_3       ATTTTACGAGTTAATCAAACCGTTGAACTAACAAATCTAAAAAAAAAAAAGGATTTGATAATGAATTCGAAACAGAAAAATGAATGGATTAGATGAAAAT
Salvia_dorrii_4          ATTTTACGAGCTAATCAAACCGTTGAACTAACAAATCG---AAAAAAAAAAGATTTGCTAATGGATTCGAAACATAAAAATAAATTGATTAGATGAAAAT
Salvia_eremostachya_5    ATTTTACGAGCTAATCAAACCGTTGAACTAACAAATCG----AAAAAAAAAGATTTGCTAATGGATTCGAAACATAAAAATAAATTGATTAGATGAAAAT
Salvia_fruticosa_6       ATTTTACGAGCTAATCAAGCCGTTGAACTAACAAATCT----AAAAAAAAAGATTTTCTAATGGATTCGAAACAT-AAAATGAATTGATTAGATGAAAAT
Salvia_huberi_7          ATTTTACGAGCTAATCAAGCCGTTGAACTAACAAATCT---AAAAAAAAAAGATTTTCTAATGGATTCGAAACAT-AAAATGAATTGATTAGATGAAAAT
Salvia_kronenburgii_8    ATTTTACGAGCTAATCAAGCCGTTGAACTAACAAATCT----AAAAAAAAAGATTTTCTAATGGATTCGAAACAT-AAAATGAATTGATTAGATGAAAAT
Salvia_limbata_9         ATTTTACGAGCTAATCAAGCCGTTGAACTAACAAATCT---AAAAAAAAAAGATTTTCTAATGGATTCGAAACAT-AAAATGAATTGATTAGATCAAAAT
Salvia_mohavensis_10     ATTTTACGAGCTAATCAAACCGTTGAACTAACAAATCG---AAAAAAAAAAGATTTGCTAATGGATTCGAAACATAAAAATAAATTGATTAGATGAAAAT
Salvia_officinalis_subsp ATTTTACGAGCTAATCAAGCCGTTGAACTAACAAATCT-AAAAAAAAAAAAGATTTTCTAATGGATTCGAAACAT-AAAATGAATTGATTAGATGAAAAT
Salvia_pachyphylla_12    ATTTTACGAGCTAATCAAACCGTTGAACTAACAAATCG----AAAAAAAAAGATTTGCTAATGGATTCGAAACATAAAAATAAATTGATTAGATGAAAAT
Salvia_pratensis_13      ATTTTACGAGCTAATCAAGCCGTTGAACTAACAAATCT----AAAAAAAAAGATTTTCTAATGGATTCGAAACAT-AAAATGAATTGATTAGATCAAAAT
Salvia_saccardiana_14    ATTTTACGAGCTAATCAAGCCGTTGAACTAACAAATCT----AAAAAAAAAGATTTTCTAATGGATTCGAAACAT-AAAATGAATTGATTAGATCAAAAT
Salvia_sonomensis_15     ATTTTACGAGCTAATCAAACCGTTGAACTAACAAATCG----AAAAAAAAAGATTTGCTAATGGATTCGAAACATAAAAATAAATTGATTAGATGAAAAT
Salvia_spathacea_16      ATTTTACGAGCTAATCAAACCGTTGAACTAACAAATCG---AAAAAAAAAAGATTTGCTAATGGATTCGAAACATAAAAATAAATTGATTAGATGAAAAT
Salvia_verbenaca_17      ATTTTACGAGCTAATCAAGCCGTTGAACTAACAAATCT--AAAAAAAAAAAGATTTTCTAATGGATTCGAAACAT-AAAATGAATTGATTAGATCAAAAT
Salvia_xanthocheila_18   ATTTTACGAGCTAATCAAGCCGTTGAACTAACAAATCT-AAAAAAAAAAAAGATTTTCTAATGGATTCGAAACAT-AAAATGAATTGATTAGATCAAAAT
                         201                                                                                                 
Salvia_aethiopis_1       ACAAGAAATTATAAGAT--------AAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCCTTTTTCAATCAAAAATCACCCTCTTCTATCA
Salvia_amarissima_2      ACAAGAAATTATCAGATAAAAGAAAAAAATAGACAGAATTTTCAAAATTGATAAAACACCTCCTATCCCTTTTTCAATCAAAAAGCACCCTCTTGTATCA
Salvia_chinensis_3       CCAAGAAATTATCAGATAAAAGAAAAAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAACCCTTTTTCAATCAAAAAGCACCCTCTTGTATCA
Salvia_dorrii_4          ACAAGAAATTATCAGATAAAAGAAAAAAATAGACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCCTTTTTCAATCAAAAAGCACCCTCTTGTATCA
Salvia_eremostachya_5    ACAAGAAATTATCAGATAAAAGAAAAAAATAGACAGAATTTTCAAAATGGATAAAACATCTCCTAGCCCTTTTTCAATCAAAAAGCACCCTCTTGTATCA
Salvia_fruticosa_6       ACAAGAAATTATCAAATAAAA-GAAAAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCTTTTTTCAATCAAAAATCACCCTCTTCTATCA
Salvia_huberi_7          ACAAGAAATTATCAAATAAAA-GAAAAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCTTTTTTCAATCAAAAATCACCCTCTTCTATCA
Salvia_kronenburgii_8    ACAAGAAATTATCAAATAAAA-GAAAAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCTTTTTTCAATCAAAAATCACCCTCTTCTATCA
Salvia_limbata_9         ACAAGAAATTATAAGATAAAATAAAAAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCTTTTTTCAATCAAAAATCACCCTCTTCTATCA
Salvia_mohavensis_10     ACAAGAAATTATCAGATAAAAGAAAAAAATAGACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCCTTTTTCAATCAAAAAGCACCCTCTTGTATCA
Salvia_officinalis_subsp ACAAGAAATTATCAAATAAAA-GAAAAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCTTTTTTCAATCAAAAATCACCCTCTTCTATCA
Salvia_pachyphylla_12    ACAAGAAATTATCAGATAAAAGAAAAAAATAGACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCCTTTTTCAATCAAAAAGCACCCTCTTGTATCA
Salvia_pratensis_13      ACAAGAAATTATAAGATAAAATAAAAAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCTTTTTTCAATCAAAAATCACCCTCTTCTATCA
Salvia_saccardiana_14    ACAAGAAATTATAAGATAAAATAAAAAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCTTTTTTCAATCAAAAATCACCCTCTTCTATCA
Salvia_sonomensis_15     ACAAGAAATTATCAGATAAAAGAAAAAAATAGACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCCTTTTTCAATCAAAAAGCACCCTCTTGTATCA
Salvia_spathacea_16      ACAAGAAATTATCAGATAAAAGAAAAAAATAGACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCCTTTTTCAATCAAAAAGCACCCTCTTGTATCA
Salvia_verbenaca_17      ACAAGAAATTATAAGATAAAATAAAAAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCTTTTTTCAATCAAAAATCACCCTCTTCTATCA
Salvia_xanthocheila_18   ACAAGAAATTATAAGATAAAATAAAAAAATATACAGAATTTTCAAAATTGATAAAACATCTCCTAGCCTTTTTTCAATCAAAAATCACCCTCTTCTATCA
                         301                                                                                                 
Salvia_aethiopis_1       AAAAAAGCATCATTTGCATAAGATTAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_amarissima_2      AAAAAAGCATCATGTGCATAAGATTAAGATAAAGTAAAAAACTACACGACATAAAGAAATAGACCCC---------TCACGAAGAATTATATTTGTTCAA
Salvia_chinensis_3       AAAAAAGCATCATGTGCATAAGATTAAGATAAAGTAAAAAACTACGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_dorrii_4          AAAAAAACATCATGTGCATAAGATTAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_eremostachya_5    AAAAAAACATCATGTG--------TAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_fruticosa_6       AAAAAAGCATCATTTGCATAAGATTAAGATAAAGTAAAAAACTACGCGATATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_huberi_7          AAAAAAGCATCATTTGCATAAGATTAAGATAAAGTAAAAAACTACGCGATATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_kronenburgii_8    AAAAAAGCATCATTTGCATAAGATTAAGATAAAGTAAAAAACTACGCGATATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_limbata_9         AAAAAAGCATCATTTGCATAAGATTAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_mohavensis_10     AAAAAAACATCATGTGCATAAGATTAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_officinalis_subsp AAAAAAGCATCATTTGCATAAGATTAAGATAAAGTAAAAAACTACGCGATATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_pachyphylla_12    AAAAAAACATCATGTGCATAAGATTAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_pratensis_13      AAAAAAGCATCATTTGCATAAGATTAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_saccardiana_14    AAAAAAGCATCATTTGCATAAGATTAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_sonomensis_15     AAAAAAACATCATGTGCATAAGATTAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_spathacea_16      AAAAAAACATCATGTGCATAAGATTAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_verbenaca_17      AAAAAAGCATCATTTGC-------TAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
Salvia_xanthocheila_18   AAAAAAGCATCATTTGCATAAGATTAAGATAAAGTAAAAAACTAGGCGACATAAAGAAATAGACCCCCTTCACTTATCACGAAGAATTATATTTGTTCAA
                         401                                                                                                 
Salvia_aethiopis_1       TACACTCTTGTCAATATAAATGTCGAAAAAAGAATACAGTGGAAAAG--------------GGGGGGATC--AAAAAAAACAAGTAAAGAAAGATTAGAC
Salvia_amarissima_2      TACACTCTTGTCAATATAAATATTGATAAAAGAATACAGTGGAAAAAGGGAAAAA-GAAAAGGGGGGATCAAAAAAAAAACAAGTAAAGAACAATTAGAC
Salvia_chinensis_3       TACACTCTTGTCAATATAAATATTGAGAAAAGAATACAGTGGAAAAAGGGAAAAATAAAAGGGGGGGATCAAAAAAAAACAAGTTAAAGAAAAATTAGAC
Salvia_dorrii_4          TACACTCTTGTCAATATAAATGTTGAGAAAAGAATACAGTGGA--------------AAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_eremostachya_5    TACACTCTTGTCAATATAAATGTTGAGAAAAGAATACAGTGGA--------------AAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_fruticosa_6       TACACTCTTGTCAATATAAATGTCGAGAAAAGAATACAGTGGAAAAGGGGAAAAA-TAAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_huberi_7          TACACTCTTGTCAATATAAATGTCGAGAAAAGAATACAGTGGAAAAGGGGAAAAA-TAAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_kronenburgii_8    TACACTCTTGTCAATATAAATGTCGAGAAAAGAATACAGTGGAAAAGGGGAAAAA-TAAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_limbata_9         TACACTCTTGTCAATATAAATGTCGAAAAAAGAATACAGTGGAAAAG--------------GGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_mohavensis_10     TACACTCTTGTCAATATAAATGTTGAGAAAAGAATACAGTGGA--------------AAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_officinalis_subsp TACACTCTTGTCAATATAAATGTCGAGAAAAGAATACAGTGGAAAAGGGGAAAAA-TAAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_pachyphylla_12    TACACTCTTGTCAATATAAATGTTGAGAAAAGAATACAGTGGA--------------AAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_pratensis_13      TACACTCTTGTCAATATAAATGTCGAAAAAAGAATACAGTGGAAAAGGGGAAAAA-TCAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_saccardiana_14    TACACTCTTGTCAATATAAATGTCGAAAAAAGAATACAGTGGAAAAGGGGAAAAA-TCAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_sonomensis_15     TACACTCTTGTCAATATAAATGTTGAGAAAAGAATACAGTGGA--------------AAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_spathacea_16      TACACTCTTGTCAATATAAATGTTGAGAAAAGAATACAGTGGA--------------AAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_verbenaca_17      TACACTCTTGTCAATATAAATGTCGAAAAAAGAATACAGTGGAAAAGGGGAAAAA-TAAAAGGGGGGATC--AAAAAAAACAAGTAAAGAAAAATTAGAC
Salvia_xanthocheila_18   TACACTCTTGTCAATATAAATGTCGAAAAAAGAATACAGTGGAAAAG--------------GGGGGGATC--AAAAAAAACAAGTAAAGAAAGATTAGAC
                         501                                                                  
Salvia_aethiopis_1       AAAGTTATATACAAGTAGCTA-CCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_amarissima_2      AAAGTTATATACAAGTCGCTACCCCCCCTTGGTATTTCTTTAATTGAATTTCATTTAATTAGACGGAAG
Salvia_chinensis_3       AAAGTTATATACAAGCCGCTACCCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACAGAAG
Salvia_dorrii_4          AAAGTTATATACAAGTCGCTACCCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_eremostachya_5    AAAGTTATATACAAGTCGCTACCCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_fruticosa_6       AAAGTTATATACAAGTAGCTACCCCCCCTTGGTATTTC-----TTTAATTTGATATGATTAGACGGAAG
Salvia_huberi_7          AAAGTTATATACAAGTAGCTACCCCCCCTTGGTATTTCTTTAATTTAATTTGATATGATTAGACGGAAG
Salvia_kronenburgii_8    AAAGTTATATACAAGTAGCTACCCCCCCTTGGTATTTCTTTAATTTAATTTGATATGATTAGACGGAAG
Salvia_limbata_9         AAAGTTATATACAAGTAGCTA-CCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_mohavensis_10     AAAGTTATATACAAGTCGCTACCCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_officinalis_subsp AAAGTTATATACAAGTAGCTACCCCCCCTTGGTATTTCTTTAATTTAATTTGATATGATTAGACGGAAG
Salvia_pachyphylla_12    AAAGTTATATACAAGTCGCTACCCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_pratensis_13      AAAGTTATATACAAGTAGCTA-CCCCCCTTGATATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_saccardiana_14    AAAGTTATATACAAGTAGCTA-CCCCCCTTGATATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_sonomensis_15     AAAGTTATATACAAGTCGCTACCCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_spathacea_16      AAAGTTATATACAAGTCGCTACCCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_verbenaca_17      AAAGTTATATACAAGTAGCTA-CCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG
Salvia_xanthocheila_18   AAAGTTATATACAAGTAGCTA-CCCCCCTTGGTATTTCTTTAATTGAATTTCATTTGATTAGACGGAAG

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTAGCCGCACTTAAAAGCC CTTTTAAAGAAGGCGGGAGT 50.0 45.0 58.3 55.7 628-652 bp 1.0 71.1
trnK-UUU-rps16_p2 CCGCACTTAAAAGCCGAGTA CTTTTAAAGAAGGCGGGAGT 50.0 45.0 58.3 55.7 623-647 bp 1.0 71.0
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC CTTTTAAAGAAGGCGGGAGTT 50.0 42.9 58.3 56.4 628-652 bp 1.0 69.4
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA CTTTTAAAGAAGGCGGGAGTT 50.0 42.9 58.3 56.4 623-647 bp 1.0 69.2
trnK-UUU-rps16_p5 TCTAGCCGCACTTAAAAGCC AGAAGGCGGGAGTTTTTAAA 50.0 40.0 58.3 55.2 621-645 bp 1.0 67.2

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