primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	trnK-rps16	1	CGCACTTAAAAGCCGAGTAC	GTGCTCAACCAACAGGAACT	20	20	50.0	50.0	57.2	58.3	0.0	0.0	1.101860	4198	5128	1010-1093	1.000000	76.7
trnK-rps16_p2	trnK-rps16	2	CGCACTTAAAAGCCGAGTAC	AGGCAGGTGTTTTTACGGAA	20	20	50.0	45.0	57.2	57.6	0.0	0.0	1.133904	4198	5128	971-1054	1.000000	76.7
trnK-rps16_p3	trnK-rps16	3	CGCACTTAAAAGCCGAGTAC	AAGGCAGGTGTTTTTACGGA	20	20	50.0	45.0	57.2	57.6	0.0	0.0	1.133904	4198	5128	972-1055	1.000000	76.6
trnK-rps16_p4	trnK-rps16	4	CGCACTTAAAAGCCGAGTAC	GGCAGGTGTTTTTACGGAAC	20	20	50.0	50.0	57.2	57.6	0.0	0.0	1.192983	4198	5128	970-1053	1.000000	76.2
trnK-rps16_p5	trnK-rps16	5	AGCCGAGTACTCTACCATTGA	GTGCTCAACCAACAGGAACT	21	20	47.6	50.0	58.0	58.3	20.3	0.0	1.333966	4198	5128	1000-1083	1.000000	74.9
rps16-trnQ_p1	rps16-trnQ	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6237	7579	1187-1434	1.000000	85.1
rps16-trnQ_p2	rps16-trnQ	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6237	7579	1198-1445	1.000000	84.6
rps16-trnQ_p3	rps16-trnQ	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6237	7579	1191-1438	1.000000	80.4
rps16-trnQ_p4	rps16-trnQ	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6237	7579	1217-1464	1.000000	80.2
rps16-trnQ_p5	rps16-trnQ	5	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.2	58.1	0.0	16.9	0.844354	6237	7579	1199-1446	1.000000	80.0
trnR-atpA_p1	trnR-atpA	1	TGGAATGAAAAGCGTCCATTG	TCCACGAAGACATTCACCAA	21	20	42.9	45.0	57.4	57.1	5.3	0.0	2.495008	10776	11252	613-755	1.000000	67.3
trnR-atpA_p2	trnR-atpA	2	TGGAATGAAAAGCGTCCATTG	TTCCACGAAGACATTCACCA	21	20	42.9	45.0	57.4	57.1	5.3	0.0	2.495008	10776	11252	614-756	1.000000	67.3
trnR-atpA_p3	trnR-atpA	3	GGAATGAAAAGCGTCCATTGT	TCCACGAAGACATTCACCAA	21	20	42.9	45.0	57.4	57.1	5.3	0.0	2.496690	10776	11252	612-754	1.000000	67.3
trnR-atpA_p4	trnR-atpA	4	GGAATGAAAAGCGTCCATTGT	TTCCACGAAGACATTCACCA	21	20	42.9	45.0	57.4	57.1	5.3	0.0	2.496690	10776	11252	613-755	1.000000	67.3
trnR-atpA_p5	trnR-atpA	5	TGGAATGAAAAGCGTCCATTG	GGAAGCCCTTTTGAAAGAAGC	21	21	42.9	47.6	57.4	58.0	5.3	5.1	2.606301	10776	11252	587-729	1.000000	66.6
rpoB-trnC_p1	rpoB-trnC	1	GATACCGGGGCTTTGCAATA	TGTTTGTTGATGAGGCGACA	20	20	50.0	45.0	58.0	58.0	6.3	0.0	0.063924	27927	29081	1635-1638	1.000000	82.0
rpoB-trnC_p2	rpoB-trnC	2	CGAGCCCATATCCTTGCTTT	TGTTTGTTGATGAGGCGACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.066711	27927	29081	1751-1754	1.000000	81.9
rpoB-trnC_p3	rpoB-trnC	3	CCGGGGCTTTGCAATATTTG	TGTTTGTTGATGAGGCGACA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.100958	27927	29081	1631-1634	1.000000	81.7
rpoB-trnC_p4	rpoB-trnC	4	GATACCGGGGCTTTGCAATA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	6.3	0.0	0.106468	27927	29081	1614-1617	1.000000	81.7
rpoB-trnC_p5	rpoB-trnC	5	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.109256	27927	29081	1730-1733	1.000000	81.6
trnC-petN_p1	trnC-petN	1	TTTCCCCAGTTCAAATCCGG	TCTAGAGTCCACTTCGTCCC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.207901	29155	30047	1004-1014	1.000000	82.0
trnC-petN_p2	trnC-petN	2	TGCAAATCCCTTTTTCCCCA	TCTAGAGTCCACTTCGTCCC	20	20	45.0	55.0	57.9	57.9	0.0	0.0	0.235002	29155	30047	1016-1026	1.000000	81.7
trnC-petN_p3	trnC-petN	3	TTTCCCCAGTTCAAATCCGG	TTCGTCCCCATACTACGAGG	20	20	50.0	55.0	58.1	58.3	0.0	0.0	0.398185	29155	30047	992-1002	1.000000	80.5
trnC-petN_p4	trnC-petN	4	TGCAAATCCCTTTTTCCCCA	TTCGTCCCCATACTACGAGG	20	20	45.0	55.0	57.9	58.3	0.0	0.0	0.425286	29155	30047	1004-1014	1.000000	80.2
trnC-petN_p5	trnC-petN	5	TTTCCCCAGTTCAAATCCGG	ACTTCGTCCCCATACTACGA	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.507271	29155	30047	994-1004	1.000000	79.6
petN-psbM_p1	petN-psbM	1	TTCCCTCGTAGTATGGGGAC	TCATTCTAGTTCCTACCGCCT	20	21	55.0	47.6	57.9	58.0	10.1	0.0	1.105782	30138	31039	984-1011	1.000000	75.2
petN-psbM_p2	petN-psbM	2	CCTCGTAGTATGGGGACGAA	TCATTCTAGTTCCTACCGCCT	20	21	55.0	47.6	58.3	58.0	1.5	0.0	1.344151	30138	31039	981-1008	1.000000	73.3
petN-psbM_p3	petN-psbM	3	TTCCCTCGTAGTATGGGGAC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	57.9	57.6	10.1	0.0	1.485111	30138	31039	998-1025	1.000000	72.1
petN-psbM_p4	petN-psbM	4	TTCCCTCGTAGTATGGGGAC	TCATTCTAGTTCCTACCGCC	20	20	55.0	50.0	57.9	56.4	10.1	0.0	1.639902	30138	31039	984-1011	1.000000	70.9
petN-psbM_p5	petN-psbM	5	TTCCCTCGTAGTATGGGGAC	CATTCTAGTTCCTACCGCCT	20	20	55.0	50.0	57.9	56.4	10.1	0.0	1.649629	30138	31039	983-1010	1.000000	70.8
psbM-trnD_p1	psbM-trnD	1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	58.0	58.5	0.0	5.4	1.505426	31145	32252	1234-1235	1.000000	70.7
psbM-trnD_p2	psbM-trnD	2	AGGCGGTAGGAACTAGAATGA	GTTCAATTGGTCAGAGCACC	21	20	47.6	50.0	58.0	57.0	0.0	5.4	2.025580	31145	32252	1235-1236	1.000000	66.6
psbM-trnD_p3	psbM-trnD	3	GGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	56.4	58.5	0.0	5.4	2.039547	31145	32252	1233-1234	1.000000	66.5
psbM-trnD_p4	psbM-trnD	4	AGGCGGTAGGAACTAGAATG	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	56.4	58.5	0.0	5.4	2.049273	31145	32252	1234-1235	1.000000	66.4
psbM-trnD_p5	psbM-trnD	5	AGGCGGTAGGAACTAGAATGA	TCAATTGGTCAGAGCACCG	21	19	47.6	52.6	58.0	57.8	0.0	5.4	2.259829	31145	32252	1233-1234	1.000000	64.7
trnE-trnT_p1	trnE-trnT	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	32851	33822	843-1115	1.000000	84.8
trnE-trnT_p2	trnE-trnT	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	32851	33822	822-1094	1.000000	84.4
trnE-trnT_p3	trnE-trnT	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	32851	33822	819-1091	1.000000	84.2
trnE-trnT_p4	trnE-trnT	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32851	33822	805-1077	1.000000	84.0
trnE-trnT_p5	trnE-trnT	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32851	33822	784-1056	1.000000	83.9
trnT-psbD_p1	trnT-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33897	34999	1252-1258	1.000000	80.3
trnT-psbD_p2	trnT-psbD	2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	20	20	55.0	50.0	58.1	57.7	7.6	9.5	0.323334	33897	34999	1266-1272	1.000000	80.1
trnT-psbD_p3	trnT-psbD	3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33897	34999	1255-1261	1.000000	80.1
trnT-psbD_p4	trnT-psbD	4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	20	20	50.0	50.0	58.1	57.7	0.0	9.5	0.345488	33897	34999	1269-1275	1.000000	79.9
trnT-psbD_p5	trnT-psbD	5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	20	20	55.0	50.0	58.1	57.7	7.6	9.5	0.371563	33897	34999	1268-1274	1.000000	79.7
psaB_p1	psaB	1	AAGATCCCCTTCTCCATCGA	ACAACATGGGCGTTCTTCTT	20	20	50.0	45.0	57.5	58.0	0.0	0.0	0.497615	39671	41875	2332-2332	1.000000	76.5
psaB_p2	psaB	2	AGATCCCCTTCTCCATCGAA	ACAACATGGGCGTTCTTCTT	20	20	50.0	45.0	57.5	58.0	0.0	0.0	0.497615	39671	41875	2331-2331	1.000000	76.5
psaB_p3	psaB	3	AAGATCCCCTTCTCCATCGA	GCAGTCGGATAATGGCTAGG	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.564926	39671	41875	2299-2299	1.000000	75.9
psaB_p4	psaB	4	AGATCCCCTTCTCCATCGAA	GCAGTCGGATAATGGCTAGG	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.564926	39671	41875	2298-2298	1.000000	75.9
psaB_p5	psaB	5	AGATCCCCTTCTCCATCGAA	GGGCGTTCTTCTTAGCAAGA	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.573634	39671	41875	2324-2324	1.000000	75.9
psaA_p1	psaA	1	AGCTAAGCCTTGGCTAAACC	GCGGGTCTCTTTGTATGTGT	20	20	50.0	50.0	57.9	57.9	14.2	0.0	0.225034	41901	44153	2359-2359	1.000000	78.6
psaA_p2	psaA	2	GCTAAGCCTTGGCTAAACCT	GCGGGTCTCTTTGTATGTGT	20	20	50.0	50.0	57.9	57.9	15.1	0.0	0.225034	41901	44153	2358-2358	1.000000	78.6
psaA_p3	psaA	3	AATACGACGAGTAGTGGGGT	GCGGGTCTCTTTGTATGTGT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.229551	41901	44153	2383-2383	1.000000	78.6
psaA_p4	psaA	4	GTAGTGGGGTCCTGAGCTAA	GCGGGTCTCTTTGTATGTGT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.232780	41901	44153	2373-2373	1.000000	78.6
psaA_p5	psaA	5	GCTAAGCCTTGGCTAAACCT	TTGGCGGGTCTCTTTGTATG	20	20	50.0	50.0	57.9	57.9	15.1	0.0	0.238222	41901	44153	2361-2361	1.000000	78.5
trnT-trnL_p1	trnT-trnL	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	48866	49827	997-1024	1.000000	82.8
trnT-trnL_p2	trnT-trnL	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	48866	49827	1029-1056	1.000000	81.8
trnT-trnL_p3	trnT-trnL	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	48866	49827	991-1018	1.000000	81.6
trnT-trnL_p4	trnT-trnL	4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	48866	49827	1029-1056	1.000000	80.6
trnT-trnL_p5	trnT-trnL	5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	48866	49827	1023-1050	1.000000	80.5
trnF-ndhJ_p1	trnF-ndhJ	1	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGGTAGGATGGCC	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.287928	50861	51562	806-812	1.000000	80.2
trnF-ndhJ_p2	trnF-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGTAGGATGGCCCTTACGTA	20	20	50.0	55.0	58.1	58.3	0.0	0.0	0.343555	50861	51562	798-804	1.000000	79.7
trnF-ndhJ_p3	trnF-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGGTAGGATGGC	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.559303	50861	51562	807-813	1.000000	78.0
trnF-ndhJ_p4	trnF-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ATGCCTGAAAGTTGGGTAGG	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.566354	50861	51562	812-818	1.000000	77.9
trnF-ndhJ_p5	trnF-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGCCTGAAAGTTGGGTAGGA	20	20	50.0	50.0	58.1	58.6	0.0	0.0	0.611278	50861	51562	811-817	1.000000	77.6
petA-psbJ_p1	petA-psbJ	1	TGAGAAGGTTCAATTGTCCGA	CCTGTGATCGGTTTAGTCGG	21	20	42.9	55.0	57.2	58.1	5.1	0.0	1.839837	65422	66420	1060-1100	1.000000	71.2
petA-psbJ_p2	petA-psbJ	2	TGAGAAGGTTCAATTGTCCGA	TCCGGCTTAGGTTCATCTCT	21	20	42.9	50.0	57.2	57.8	5.1	0.0	1.923128	65422	66420	1018-1058	1.000000	70.7
petA-psbJ_p3	petA-psbJ	3	TGAGAAGGTTCAATTGTCCGA	CCGGCTTAGGTTCATCTCTG	21	20	42.9	55.0	57.2	57.8	5.1	0.0	1.993622	65422	66420	1017-1057	1.000000	70.2
petA-psbJ_p4	petA-psbJ	4	TGAGAAGGTTCAATTGTCCGAA	CCTGTGATCGGTTTAGTCGG	22	20	40.9	55.0	57.9	58.1	5.1	0.0	2.157409	65422	66420	1060-1100	1.000000	68.6
petA-psbJ_p5	petA-psbJ	5	TTGAGAAGGTTCAATTGTCCGA	CCTGTGATCGGTTTAGTCGG	22	20	40.9	55.0	57.9	58.1	5.1	0.0	2.157409	65422	66420	1061-1101	1.000000	68.6
trnR-trnN_p1	trnR-trnN	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	110951	111567	760-760	1.000000	79.3
trnR-trnN_p2	trnR-trnN	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	110951	111567	797-797	1.000000	78.7
trnR-trnN_p3	trnR-trnN	3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	110951	111567	711-711	1.000000	78.1
trnR-trnN_p4	trnR-trnN	4	CAGAGGATTAGAGCACGTGG	GGGTTGAGAACGGGAATTGA	20	20	55.0	50.0	57.8	57.8	18.9	0.0	0.410108	110951	111567	800-800	1.000000	77.7
trnR-trnN_p5	trnR-trnN	5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.7	58.2	18.7	18.9	0.442458	110951	111567	748-748	1.000000	77.5
rpl32-trnL_p1	rpl32-trnL	1	TCAACGGGGAATTCACAAAGT	AGCGTGTCTACCGATTTCAC	21	20	42.9	50.0	58.1	58.0	10.5	0.0	1.068992	116183	117040	922-943	1.000000	75.3
rpl32-trnL_p2	rpl32-trnL	2	TCAACGGGGAATTCACAAAGT	CAGCGTGTCTACCGATTTCA	21	20	42.9	50.0	58.1	58.0	10.5	0.0	1.070363	116183	117040	923-944	1.000000	75.3
rpl32-trnL_p3	rpl32-trnL	3	TCAACGGGGAATTCACAAAGT	TCCTAAGAGCAGCGTGTCTA	21	20	42.9	50.0	58.1	57.9	10.5	0.0	1.185439	116183	117040	932-953	1.000000	74.3
rpl32-trnL_p4	rpl32-trnL	4	TCAACGGGGAATTCACAAAGT	CCTAAGAGCAGCGTGTCTAC	21	20	42.9	55.0	58.1	57.8	10.5	0.0	1.257457	116183	117040	931-952	1.000000	73.8
rpl32-trnL_p5	rpl32-trnL	5	TCAACGGGGAATTCACAAAGT	CTAAGAGCAGCGTGTCTACC	21	20	42.9	55.0	58.1	57.8	10.5	0.0	1.257457	116183	117040	930-951	1.000000	73.8
rps15-ycf1_p1	rps15-ycf1	1	ATGAGTCGAAGAACACCGTG	TCCCCTAGAGGTCGTAGAGA	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.253232	126375	126775	1164-1176	1.000000	80.4
rps15-ycf1_p2	rps15-ycf1	2	CATTGATGGGTGGTGAGGTC	TCCCCTAGAGGTCGTAGAGA	20	20	55.0	55.0	58.2	57.8	0.0	0.0	0.432060	126375	126775	1144-1156	1.000000	78.9
rps15-ycf1_p3	rps15-ycf1	3	CCCAACTGTTCTGGACCATT	TCCCCTAGAGGTCGTAGAGA	20	20	50.0	55.0	57.7	57.8	0.0	0.0	0.480067	126375	126775	1347-1359	1.000000	78.4
rps15-ycf1_p4	rps15-ycf1	4	TGCGAAATCACTGAGCTGAA	TCCCCTAGAGGTCGTAGAGA	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.355565	126375	126775	742-743	0.714286	67.6
rps15-ycf1_p5	rps15-ycf1	5	TTGCGAAATCACTGAGCTGA	TCCCCTAGAGGTCGTAGAGA	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.355565	126375	126775	743-744	0.714286	67.6
ycf1_p1	ycf1	1	TAAGGTCCGCTCAACAACG	TGGAATTATCTCAATGAACAAAA	19	23	52.6	26.1	57.8	52.6	0.0	0.0	9.601678	130798	130917	188-188	1.000000	44.3
ycf1_p2	ycf1	2	CGATAAGGTCCGCTCAACAA	TGGAATTATCTCAATGAACAAAA	20	23	50.0	26.1	58.0	52.6	0.0	0.0	8.401755	130798	130917	191-191	1.000000	44.2
ycf1_p3	ycf1	3	CGATAAGGTCCGCTCAACAA	TGGAATTATCTCAATGAACAAAAA	20	24	50.0	25.0	58.0	53.4	0.0	0.0	8.575335	130798	130917	191-191	1.000000	44.2
ycf1_p4	ycf1	4	ATAAGGTCCGCTCAACAACG	TGGAATTATCTCAATGAACAAAA	20	23	50.0	26.1	58.3	52.6	0.0	0.0	8.675161	130798	130917	189-189	1.000000	44.2
ycf1_p5	ycf1	5	ATAAGGTCCGCTCAACAACG	TGGAATTATCTCAATGAACAAAAA	20	24	50.0	25.0	58.3	53.4	0.0	0.0	8.848741	130798	130917	189-189	1.000000	44.2