primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	AATGAGGGAGGAGGTTTGGT	GTGCTTGGGAGTCCTTGAAA	20	20	50.0	50.0	58.2	58.0	0.0	9.6	0.258339	73	1134	1166-1166	1.000000	77.9
psbA_p2	psbA	2	AATGAGGGAGGAGGTTTGGT	TAGCTAATACGCGTGCTTGG	20	20	50.0	50.0	58.2	58.2	0.0	24.2	0.391777	73	1134	1178-1178	1.000000	76.9
psbA_p3	psbA	3	ATGAGGGAGGAGGTTTGGTA	GTGCTTGGGAGTCCTTGAAA	20	20	50.0	50.0	57.3	58.0	0.0	9.6	0.679920	73	1134	1165-1165	1.000000	74.6
psbA_p4	psbA	4	TGAGGGAGGAGGTTTGGTAT	GTGCTTGGGAGTCCTTGAAA	20	20	50.0	50.0	57.3	58.0	0.0	9.6	0.679920	73	1134	1164-1164	1.000000	74.6
psbA_p5	psbA	5	TGAGGGAGGAGGTTTGGTAT	TAGCTAATACGCGTGCTTGG	20	20	50.0	50.0	57.3	58.2	0.0	24.2	0.813358	73	1134	1176-1176	1.000000	73.5
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	TAGGCTTGTACTTTCGCGTC	CATAGGGAAAGTCGTGTGCA	20	20	50.0	50.0	58.0	57.9	5.8	0.0	0.106608	1135	1394	414-418	1.000000	79.9
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TAGGCTTGTACTTTCGCGTC	ACATAGGGAAAGTCGTGTGC	20	20	50.0	50.0	58.0	57.9	5.8	0.0	0.108064	1135	1394	415-419	1.000000	79.9
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	TAGGCTTGTACTTTCGCGTC	TCGTGTGCAATGAAAACTGC	20	20	50.0	45.0	58.0	57.9	5.8	0.0	0.131531	1135	1394	403-407	1.000000	79.7
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	TAGGCTTGTACTTTCGCGTC	AATGAAAACTGCAAGCACGG	20	20	50.0	45.0	58.0	57.9	5.8	0.0	0.151898	1135	1394	395-399	1.000000	79.6
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	TAGGCTTGTACTTTCGCGTC	CGTGTGCAATGAAAACTGCA	20	20	50.0	45.0	58.0	58.2	5.8	0.0	0.175654	1135	1394	402-406	1.000000	79.4
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCATGATCATGAGCAAGTGC	TCTGGGTTGCTAACTCAACG	20	20	50.0	50.0	57.2	57.8	32.7	3.7	0.940130	3218	3982	912-912	1.000000	72.5
matK-trnK-UUU_p2	matK-trnK-UUU	2	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	3218	3982	896-896	1.000000	72.4
matK-trnK-UUU_p3	matK-trnK-UUU	3	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	3218	3982	895-895	1.000000	72.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	57.2	57.5	32.7	0.0	1.237069	3218	3982	909-909	1.000000	70.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	CCATGATCATGAGCAAGTGC	CTGGGTTGCTAACTCAACGG	20	20	50.0	55.0	57.2	58.8	32.7	3.7	1.619576	3218	3982	911-911	1.000000	67.0
rps2_p1	rps2	1	TGAAATCCAGGAATAGCCAA	CAACGGTCAATCCTCGGTAG	20	20	40.0	55.0	54.3	58.1	0.0	0.0	3.794235	16717	17499	944-968	1.000000	51.7
rps2_p2	rps2	2	TTGAAATCCAGGAATAGCCA	CAACGGTCAATCCTCGGTAG	20	20	40.0	55.0	54.3	58.1	0.0	0.0	3.794235	16717	17499	945-969	1.000000	51.7
rps2_p3	rps2	3	TGAAATCCAGGAATAGCCAA	AACGGTCAATCCTCGGTAGA	20	20	40.0	50.0	54.3	58.2	0.0	0.0	3.873437	16717	17499	943-967	1.000000	51.0
rps2_p4	rps2	4	TGAAATCCAGGAATAGCCAA	ACGGTCAATCCTCGGTAGAA	20	20	40.0	50.0	54.3	58.2	0.0	0.0	3.873437	16717	17499	942-966	1.000000	51.0
rps2_p5	rps2	5	TTGAAATCCAGGAATAGCCA	ACGGTCAATCCTCGGTAGAA	20	20	40.0	50.0	54.3	58.2	0.0	0.0	3.873437	16717	17499	943-967	1.000000	51.0
rpoC2_p1	rpoC2	1	TGAGTCTCCTTTTAGGAATCA	TATAATCCCGATCCGTGCAG	21	20	38.1	50.0	53.6	57.0	0.0	0.0	6.432134	17751	18750	1066-1066	1.000000	40.0
rpoC2_p2	rpoC2	2	TGAGTCTCCTTTTAGGAATCA	TTATAATCCCGATCCGTGCAG	21	21	38.1	47.6	53.6	57.7	0.0	0.0	6.714996	17751	18750	1067-1067	1.000000	40.0
rpoC2_p3	rpoC2	3	ATGAGTCTCCTTTTAGGAATCA	TATAATCCCGATCCGTGCAG	22	20	36.4	50.0	54.2	57.0	0.0	0.0	6.792622	17751	18750	1067-1067	1.000000	40.0
rpoC2_p4	rpoC2	4	TGAGTCTCCTTTTAGGAATCAT	TATAATCCCGATCCGTGCAG	22	20	36.4	50.0	54.2	57.0	0.0	0.0	6.792622	17751	18750	1066-1066	1.000000	40.0
rpoC2_p5	rpoC2	5	TGAGTCTCCTTTTAGGAATCA	TTATAATCCCGATCCGTGCA	21	20	38.1	45.0	53.6	56.5	0.0	0.0	6.904982	17751	18750	1067-1067	1.000000	40.0
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	AGGAAAGCGGCGAATTTTTG	TCTAGCGGTTCAGGACATCT	20	20	45.0	50.0	57.9	57.9	13.0	0.0	0.209970	33130	33355	389-391	1.000000	78.7
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	CAAAGGAAAGCGGCGAATTT	TCTAGCGGTTCAGGACATCT	20	20	45.0	50.0	57.9	57.9	13.0	0.0	0.209970	33130	33355	392-394	1.000000	78.7
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	AGGAAAGCGGCGAATTTTTG	GTCTAGCGGTTCAGGACATC	20	20	45.0	55.0	57.9	57.8	13.0	0.0	0.275926	33130	33355	390-392	1.000000	78.2
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	CAAAGGAAAGCGGCGAATTT	GTCTAGCGGTTCAGGACATC	20	20	45.0	55.0	57.9	57.8	13.0	0.0	0.275926	33130	33355	393-395	1.000000	78.2
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	AGGAAAGCGGCGAATTTTTG	TATCGTCTAGCGGTTCAGGA	20	20	45.0	50.0	57.9	57.7	13.0	0.0	0.401518	33130	33355	394-396	1.000000	77.2
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TCCCGATGGTTGGTCAAGTA	CGCATCTTTTCCTTGGCAAG	20	20	50.0	50.0	58.4	58.0	0.0	20.3	0.366390	38274	38611	490-490	1.000000	77.1
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TCCCGATGGTTGGTCAAGTA	CATTACTTCACCATGGGCGA	20	20	50.0	50.0	58.4	58.0	0.0	18.1	0.390046	38274	38611	527-527	1.000000	76.9
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTTGCTTCTCCCGATGGTTG	CGCATCTTTTCCTTGGCAAG	20	20	50.0	50.0	58.5	58.0	0.0	20.3	0.479677	38274	38611	498-498	1.000000	76.2
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	CCCGATGGTTGGTCAAGTAA	CGCATCTTTTCCTTGGCAAG	20	20	50.0	50.0	57.5	58.0	0.0	20.3	0.496317	38274	38611	489-489	1.000000	76.0
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTTGCTTCTCCCGATGGTTG	CATTACTTCACCATGGGCGA	20	20	50.0	50.0	58.5	58.0	0.0	18.1	0.503332	38274	38611	535-535	1.000000	76.0
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	CTTGCCAAGGAAAAGATGCG	GCAAGATTGGTTGGATTGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027637	38683	38844	1156-1274	1.000000	79.8
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	TATGACTCACGCCCCTCTAG	GCAAGATTGGTTGGATTGGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.031675	38683	38844	2486-2604	1.000000	79.8
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	CTTGCCAAGGAAAAGATGCG	GCAACAAGGTCCAGTTGGTA	20	20	50.0	50.0	58.0	58.0	0.0	21.7	0.037642	38683	38844	589-707	1.000000	79.8
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	CTTGCCAAGGAAAAGATGCG	GTAATAGTGCACCCACACGT	20	20	50.0	50.0	58.0	57.9	0.0	14.6	0.102252	38683	38844	717-835	1.000000	79.3
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	ATCCCGCCGATGCATTTTAT	GCAACAAGGTCCAGTTGGTA	20	20	45.0	50.0	58.1	58.0	0.0	21.7	0.114641	38683	38844	1233-1351	1.000000	79.1
trnM-CAU-trnV-UAC_p1	trnM-CAU-trnV-UAC	1	GAACCAATGACTATCGCCGT	CTCCCTTGAAAAGCATTGGC	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.439240	57108	57577	588-756	1.000000	76.7
trnM-CAU-trnV-UAC_p2	trnM-CAU-trnV-UAC	2	TGAACCAATGACTATCGCCG	CTCCCTTGAAAAGCATTGGC	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.440584	57108	57577	589-757	1.000000	76.7
trnM-CAU-trnV-UAC_p3	trnM-CAU-trnV-UAC	3	GAACCAATGACTATCGCCGT	CGTGTAAACGAGGTGCTCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.688384	57108	57577	565-733	1.000000	74.7
trnM-CAU-trnV-UAC_p4	trnM-CAU-trnV-UAC	4	TGAACCAATGACTATCGCCG	CGTGTAAACGAGGTGCTCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.689727	57108	57577	566-734	1.000000	74.7
trnM-CAU-trnV-UAC_p5	trnM-CAU-trnV-UAC	5	CCCATCGATTCCGGGATCTA	CTCCCTTGAAAAGCATTGGC	20	20	55.0	50.0	58.4	57.6	20.7	0.0	0.818074	57108	57577	638-806	1.000000	73.7
rbcL-accD_p1	rbcL-accD	1	ACCGCGCATGCTAGATAAAA	TGCCTGTGCCTTCTTTCATT	20	20	45.0	45.0	58.1	58.0	17.1	0.0	0.052248	61652	61771	1826-2216	1.000000	84.0
rbcL-accD_p2	rbcL-accD	2	ACCGCGCATGCTAGATAAAA	TTGATGCCTGTGCCTTCTTT	20	20	45.0	45.0	58.1	58.0	17.1	0.0	0.052248	61652	61771	1830-2220	1.000000	84.0
rbcL-accD_p3	rbcL-accD	3	ACCGCGCATGCTAGATAAAA	TTTGATGCCTGTGCCTTCTT	20	20	45.0	45.0	58.1	58.0	17.1	0.0	0.052248	61652	61771	1831-2221	1.000000	84.0
rbcL-accD_p4	rbcL-accD	4	AAAAGCGAAGAGAGCATCGT	TGCCTGTGCCTTCTTTCATT	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.086406	61652	61771	1591-1981	1.000000	83.8
rbcL-accD_p5	rbcL-accD	5	ACCGCGCATGCTAGATAAAA	ATGTGCTCCCTCCTTCTCAT	20	20	45.0	50.0	58.1	58.1	17.1	0.0	0.161343	61652	61771	909-993	1.000000	83.5
petA-psbJ_p1	petA-psbJ	1	CGCTACGCATTCAAGGTCTT	AACCGGTATTCCTGTGATCG	20	20	50.0	50.0	58.6	57.4	0.0	8.0	1.237056	69662	70654	1184-1185	1.000000	70.2
petA-psbJ_p2	petA-psbJ	2	CGCTACGCATTCAAGGTCTT	CGGTATTCCTGTGATCGGTT	20	20	50.0	50.0	58.6	57.4	0.0	0.0	1.237056	69662	70654	1181-1182	1.000000	70.2
petA-psbJ_p3	petA-psbJ	3	CGCTACGCATTCAAGGTCTT	TCCGGATTAGGCTCATCTCT	20	20	50.0	50.0	58.6	57.3	0.0	0.0	1.325258	69662	70654	1132-1133	1.000000	69.5
petA-psbJ_p4	petA-psbJ	4	CGCTACGCATTCAAGGTCTT	CCGGATTAGGCTCATCTCTG	20	20	50.0	55.0	58.6	57.3	0.0	8.5	1.380037	69662	70654	1131-1132	1.000000	69.0
petA-psbJ_p5	petA-psbJ	5	ATCCGCTACGCATTCAAGG	CGGTATTCCTGTGATCGGTT	19	20	52.6	50.0	58.0	57.4	0.0	0.0	1.604814	69662	70654	1184-1185	1.000000	67.2
rps18_p1	rps18	1	CGTTCGGATCTTAAATGCATGA	AGTTTTGAGCCAATTGAAGAGT	22	22	40.9	36.4	57.2	56.5	0.0	7.1	6.344210	74414	74962	538-634	1.000000	44.0
rps18_p2	rps18	2	TCGTTCGGATCTTAAATGCATG	AGTTTTGAGCCAATTGAAGAGT	22	22	40.9	36.4	57.2	56.5	0.0	7.1	6.344210	74414	74962	539-635	1.000000	44.0
rps18_p3	rps18	3	TCGTTCGGATCTTAAATGCA	AGTTTTGAGCCAATTGAAGAGT	20	22	40.0	36.4	55.2	56.5	0.0	7.1	6.358730	74414	74962	539-635	1.000000	44.0
rps18_p4	rps18	4	TTCGTTCGGATCTTAAATGCA	AGTTTTGAGCCAATTGAAGAGT	21	22	38.1	36.4	56.0	56.5	0.0	7.1	6.559292	74414	74962	540-636	1.000000	44.0
rps18_p5	rps18	5	CGTTCGGATCTTAAATGCATGA	TGAAGTTTTGAGCCAATTGAAGA	22	23	40.9	34.8	57.2	57.1	0.0	7.1	6.644804	74414	74962	541-637	1.000000	44.0
clpP-psbB_p1	clpP-psbB	1	TCATCTTCCTTGCCATCCTT	GTATAGAGCCATTGAGCCGG	20	20	45.0	55.0	56.5	57.9	0.0	0.0	1.594416	77312	78306	1182-1186	1.000000	67.5
clpP-psbB_p2	clpP-psbB	2	TTCATCTTCCTTGCCATCCT	GTATAGAGCCATTGAGCCGG	20	20	45.0	55.0	56.5	57.9	0.0	0.0	1.594416	77312	78306	1183-1187	1.000000	67.5
clpP-psbB_p3	clpP-psbB	3	CCAAGTTTCATCTTCCTTGCC	GTATAGAGCCATTGAGCCGG	21	20	47.6	55.0	57.4	57.9	0.0	0.0	1.668768	77312	78306	1189-1193	1.000000	66.9
clpP-psbB_p4	clpP-psbB	4	CCCAAGTTTCATCTTCCTTGC	GTATAGAGCCATTGAGCCGG	21	20	47.6	55.0	57.4	57.9	0.0	0.0	1.668768	77312	78306	1190-1194	1.000000	66.9
clpP-psbB_p5	clpP-psbB	5	TCATCTTCCTTGCCATCCTT	CGATACCAAGGCAAACCCAT	20	20	45.0	50.0	56.5	58.2	0.0	0.0	1.745654	77312	78306	1082-1086	1.000000	66.3
rps3_p1	rps3	1	TCACTGGATAGAATGGTAAGACA	AGCGACCCACCTGTCATATA	23	20	39.1	50.0	56.5	57.9	0.0	0.0	4.618643	88623	89309	842-842	1.000000	43.1
rps3_p2	rps3	2	TCACTGGATAGAATGGTAAGACAA	AGCGACCCACCTGTCATATA	24	20	37.5	50.0	57.1	57.9	0.0	0.0	4.949659	88623	89309	842-842	1.000000	40.4
rps3_p3	rps3	3	CACTGGATAGAATGGTAAGACA	AGCGACCCACCTGTCATATA	22	20	40.9	50.0	55.0	57.9	0.0	0.0	5.081836	88623	89309	841-841	1.000000	40.0
rps3_p4	rps3	4	TGGTAAGACAAATAAAGGTCCA	AGCGACCCACCTGTCATATA	22	20	36.4	50.0	55.0	57.9	0.0	0.0	5.084637	88623	89309	829-829	1.000000	40.0
rps3_p5	rps3	5	ATCACTGGATAGAATGGTAAGACA	AGCGACCCACCTGTCATATA	24	20	37.5	50.0	56.9	57.9	0.0	0.0	5.144628	88623	89309	843-843	1.000000	40.0
rpl22_p1	rpl22	1	TGCGAACCAAAGGGAATGAT	ATTGGTGGAAAAGGGAGTCG	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.414749	89356	89739	544-544	1.000000	76.8
rpl22_p2	rpl22	2	TGCGAACCAAAGGGAATGAT	GTGGAAAAGGGAGTCGCTTT	20	20	45.0	50.0	57.8	58.4	0.0	25.0	0.608623	89356	89739	540-540	1.000000	75.3
rpl22_p3	rpl22	3	TGCGAACCAAAGGGAATGAT	GATCTCGTCGCCAATCTCAT	20	20	45.0	50.0	57.8	57.6	0.0	0.0	0.644922	89356	89739	602-602	1.000000	75.0
rpl22_p4	rpl22	4	GCGAACCAAAGGGAATGATG	ATTGGTGGAAAAGGGAGTCG	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.767325	89356	89739	543-543	1.000000	74.0
rpl22_p5	rpl22	5	TGCGAACCAAAGGGAATGAT	AGATCTCGTCGCCAATCTCA	20	20	45.0	50.0	57.8	58.6	0.0	0.0	0.820773	89356	89739	603-603	1.000000	73.6
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	TGAACCGAATTTGATCCACGA	20	21	50.0	42.9	58.0	58.0	7.6	0.0	1.056169	92543	92912	429-510	1.000000	77.6
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	CTGAACCGAATTTGATCCACG	20	21	50.0	47.6	58.0	57.6	7.6	0.0	1.398888	92543	92912	430-511	1.000000	74.8
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	AGTTGGGCGCTTTAACCATT	TGAACCGAATTTGATCCACGA	20	21	45.0	42.9	58.4	58.0	7.6	0.0	1.419104	92543	92912	430-511	1.000000	74.7
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	TTGGGCGCTTTAACCATTCA	TGAACCGAATTTGATCCACGA	20	21	45.0	42.9	58.4	58.0	7.6	0.0	1.424976	92543	92912	428-509	1.000000	74.6
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GTTGGGCGCTTTAACCATTC	TGAACCGAATTTGATCCACG	20	20	50.0	45.0	58.0	56.4	7.6	0.0	1.565076	92543	92912	429-510	1.000000	73.5
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	TGAACCGAATTTGATCCACGA	GTTGGGCGCTTTAACCATTC	21	20	42.9	50.0	58.0	58.0	0.0	7.6	1.056169	172781	173150	429-510	1.000000	77.6
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	CTGAACCGAATTTGATCCACG	GTTGGGCGCTTTAACCATTC	21	20	47.6	50.0	57.6	58.0	0.0	7.6	1.398888	172781	173150	430-511	1.000000	74.8
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	TGAACCGAATTTGATCCACGA	AGTTGGGCGCTTTAACCATT	21	20	42.9	45.0	58.0	58.4	0.0	7.6	1.419104	172781	173150	430-511	1.000000	74.7
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	TGAACCGAATTTGATCCACGA	TTGGGCGCTTTAACCATTCA	21	20	42.9	45.0	58.0	58.4	0.0	7.6	1.424976	172781	173150	428-509	1.000000	74.6
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	TGAACCGAATTTGATCCACG	GTTGGGCGCTTTAACCATTC	20	20	45.0	50.0	56.4	58.0	0.0	7.6	1.565076	172781	173150	429-510	1.000000	73.5