primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	rps16	1	CTTGTCGGTTTAGCCCCTTT	CGTGCGACTTGAAGGACATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.103563	608	727	916-1010	1.000000	87.3
rps16_p2	rps16	2	TTCCTTCTCTTCGGGATCGA	CGTGCGACTTGAAGGACATA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.148524	608	727	813-907	1.000000	87.3
rps16_p3	rps16	3	CGGTTTAGCCCCTTTGTCAA	CGTGCGACTTGAAGGACATA	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.397682	608	727	911-1005	1.000000	84.9
rps16_p4	rps16	4	TTGTCGGTTTAGCCCCTTTG	CGTGCGACTTGAAGGACATA	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.397682	608	727	915-1009	1.000000	84.9
rps16_p5	rps16	5	AGTTCTTGTCGGTTTAGCCC	CGTGCGACTTGAAGGACATA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.189633	608	727	920-1014	0.750000	74.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TATGTCCTTCAAGTCGCACG	CCTTACCACTTGGCCACG	20	18	50.0	61.1	58.0	58.0	0.0	3.9	2.027893	992	1327	390-490	1.000000	75.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TATGTCCTTCAAGTCGCACG	GTTGCCTTACCACTTGGCC	20	19	50.0	57.9	58.0	59.0	0.0	0.0	2.044753	992	1327	394-494	1.000000	75.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CCTTACCACTTGGCCACG	20	18	45.0	61.1	58.1	58.0	8.1	3.9	2.158016	992	1327	380-480	1.000000	74.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GTTGCCTTACCACTTGGCC	20	19	45.0	57.9	58.1	59.0	8.1	0.0	2.174876	992	1327	384-484	1.000000	74.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TATGTCCTTCAAGTCGCACG	GCCTTACCACTTGGCCAC	20	18	50.0	61.1	58.0	58.3	0.0	3.9	2.327728	992	1327	391-491	1.000000	72.8
trnC-GCA-trnY-GUA_p1	trnC-GCA-trnY-GUA	1	CCCCTACTTGCTGCATCAAA	CATAACCTTGAGACCACGGG	20	20	50.0	55.0	58.2	58.0	1.8	0.0	0.186063	2342	2838	1273-1477	1.000000	86.4
trnC-GCA-trnY-GUA_p2	trnC-GCA-trnY-GUA	2	CCCCTACTTGCTGCATCAAA	CTTGAGACCACGGGTTCAAA	20	20	50.0	50.0	58.2	58.0	1.8	14.8	0.200094	2342	2838	1267-1471	1.000000	86.3
trnC-GCA-trnY-GUA_p3	trnC-GCA-trnY-GUA	3	CCCCTACTTGCTGCATCAAA	GGAGGTAACGGGGATTCAAC	20	20	50.0	55.0	58.2	58.0	1.8	0.0	0.195992	2342	2838	1113-1313	1.000000	86.2
trnC-GCA-trnY-GUA_p4	trnC-GCA-trnY-GUA	4	CCCCTACTTGCTGCATCAAA	AAGGAGGTAACGGGGATTCA	20	20	50.0	50.0	58.2	58.0	1.8	0.0	0.200755	2342	2838	1115-1315	1.000000	86.2
trnC-GCA-trnY-GUA_p5	trnC-GCA-trnY-GUA	5	CCCCTACTTGCTGCATCAAA	AGGAGGTAACGGGGATTCAA	20	20	50.0	50.0	58.2	58.0	1.8	0.0	0.200755	2342	2838	1114-1314	1.000000	86.2
trnE-UUC-trnfM-CAU_p1	trnE-UUC-trnfM-CAU	1	TGTCCTGAACCACTAGACGA	CTTGAGACCACGGGTTCAAA	20	20	50.0	50.0	57.7	58.0	0.0	14.8	0.319745	3089	3168	140-151	1.000000	83.5
trnE-UUC-trnfM-CAU_p2	trnE-UUC-trnfM-CAU	2	TGTCCTGAACCACTAGACGA	CATAACCTTGAGACCACGGG	20	20	50.0	55.0	57.7	58.0	0.0	0.0	0.305715	3089	3168	146-157	1.000000	83.4
trnE-UUC-trnfM-CAU_p3	trnE-UUC-trnfM-CAU	3	TGTCCTGAACCACTAGACGA	GTTCAAATCCTGTCTCCGCA	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.473128	3089	3168	127-138	1.000000	82.9
trnE-UUC-trnfM-CAU_p4	trnE-UUC-trnfM-CAU	4	TGTCCTGAACCACTAGACGA	CACGGGTTCAAATCCTGTCT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.466543	3089	3168	132-143	1.000000	82.7
trnE-UUC-trnfM-CAU_p5	trnE-UUC-trnfM-CAU	5	TGTCCTGAACCACTAGACGA	TGAGACCACGGGTTCAAATC	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.460451	3089	3168	138-149	1.000000	82.5
trnfM-CAU-rps14_p1	trnfM-CAU-rps14	1	CCCGTGGTCTCAAGGTTATG	CACGTAATAGTGCGCGGATA	20	20	55.0	50.0	58.0	58.0	0.0	8.8	0.064890	3243	3393	342-377	1.000000	91.5
trnfM-CAU-rps14_p2	trnfM-CAU-rps14	2	CCCGTGGTCTCAAGGTTATG	ACGTAATAGTGCGCGGATAC	20	20	55.0	50.0	58.0	58.0	0.0	8.8	0.066279	3243	3393	341-376	1.000000	91.5
trnfM-CAU-rps14_p3	trnfM-CAU-rps14	3	AAGGTTATGAGCCTTGCGAG	CACGTAATAGTGCGCGGATA	20	20	50.0	50.0	58.0	58.0	0.0	8.8	0.070553	3243	3393	331-366	1.000000	91.4
trnfM-CAU-rps14_p4	trnfM-CAU-rps14	4	AAGGTTATGAGCCTTGCGAG	ACGTAATAGTGCGCGGATAC	20	20	50.0	50.0	58.0	58.0	0.0	8.8	0.071942	3243	3393	330-365	1.000000	91.4
trnfM-CAU-rps14_p5	trnfM-CAU-rps14	5	GTTGAATCCCCGTTACCTCC	CACGTAATAGTGCGCGGATA	20	20	55.0	50.0	58.0	58.0	0.0	8.8	0.074819	3243	3393	502-546	1.000000	90.1
rpl33-rps18_p1	rpl33-rps18	1	CGAACTGAACTAAGAGCGCT	TCGTCGTTTGTGGATCACTC	20	20	50.0	50.0	58.0	57.9	11.3	0.0	0.076353	7678	7831	1162-1271	1.000000	89.6
rpl33-rps18_p2	rpl33-rps18	2	GTTTTGGAGACCCACGTTCT	TCGTCGTTTGTGGATCACTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.097154	7678	7831	1184-1293	1.000000	89.4
rpl33-rps18_p3	rpl33-rps18	3	GAAAAGGAGGTTCATGGCCA	TCGTCGTTTGTGGATCACTC	20	20	50.0	50.0	58.1	57.9	8.6	0.0	0.136970	7678	7831	979-1043	1.000000	89.2
rpl33-rps18_p4	rpl33-rps18	4	TGAAAAGGAGGTTCATGGCC	TCGTCGTTTGTGGATCACTC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.136970	7678	7831	980-1044	1.000000	89.2
rpl33-rps18_p5	rpl33-rps18	5	AAAGGAGGTTCATGGCCAAG	TCGTCGTTTGTGGATCACTC	20	20	50.0	50.0	58.1	57.9	11.0	0.0	0.131458	7678	7831	977-1041	0.750000	78.0
clpP-rps11_p1	clpP-rps11	1	CTTTTGGAACACCAATGGGC	GGATGTCGTCCCCCTAAAAA	20	20	50.0	50.0	57.8	57.2	3.6	0.0	0.961614	11536	11811	321-526	1.000000	83.9
clpP-rps11_p2	clpP-rps11	2	TTTGGAACACCAATGGGCAT	GGATGTCGTCCCCCTAAAAA	20	20	45.0	50.0	58.3	57.2	3.6	0.0	1.066226	11536	11811	319-524	1.000000	83.1
clpP-rps11_p3	clpP-rps11	3	CTTTTGGAACACCAATGGGC	ACATAATGGATGTCGTCCCC	20	20	50.0	50.0	57.8	57.1	3.6	8.7	1.099152	11536	11811	328-533	1.000000	82.6
clpP-rps11_p4	clpP-rps11	4	TCCAGGACTCCGAAAAGGTA	GGATGTCGTCCCCCTAAAAA	20	20	50.0	50.0	57.7	57.2	0.0	0.0	1.107359	11536	11811	341-546	1.000000	82.4
clpP-rps11_p5	clpP-rps11	5	CCAGGACTCCGAAAAGGTAC	GGATGTCGTCCCCCTAAAAA	20	20	55.0	50.0	57.6	57.2	0.0	0.0	1.175013	11536	11811	340-545	1.000000	81.8
rpl16-rps3_p1	rpl16-rps3	1	CCTCGGCAAGATATTCCCTT	TCAAAATTTGGATATTTGTAAACCCA	20	26	50.0	26.9	57.1	56.3	0.0	5.8	8.611126	14194	15366	1227-1319	1.000000	50.0
rpl16-rps3_p2	rpl16-rps3	2	GCCTCGGCAAGATATTCCC	TCAAAATTTGGATATTTGTAAACCCA	19	26	57.9	26.9	58.1	56.3	2.4	5.8	8.738087	14194	15366	1228-1320	1.000000	50.0
rpl16-rps3_p3	rpl16-rps3	3	CCTCGGCAAGATATTCCCTTC	TCAAAATTTGGATATTTGTAAACCCA	21	26	52.4	26.9	58.2	56.3	0.0	5.8	8.899883	14194	15366	1227-1319	1.000000	50.0
rpl16-rps3_p4	rpl16-rps3	4	CCTCGGCAAGATATTCCCTT	CAAAATTTGGATATTTGTAAACCCA	20	25	50.0	28.0	57.1	55.0	0.0	0.0	8.904404	14194	15366	1226-1318	1.000000	50.0
rpl16-rps3_p5	rpl16-rps3	5	GCCTCGGCAAGATATTCCC	CAAAATTTGGATATTTGTAAACCCA	19	25	57.9	28.0	58.1	55.0	2.4	0.0	9.031365	14194	15366	1227-1319	1.000000	50.0
rrn16-rrn23_p1	rrn16-rrn23	1	GGCTGGATCACCTCCTTT	GTCTCTGGGTGCCTAGGTAT	18	20	55.6	55.0	56.2	57.9	0.0	11.3	3.927440	28739	29210	494-682	1.000000	60.6
rrn16-rrn23_p2	rrn16-rrn23	2	GGCTGGATCACCTCCTTT	TCTCTGGGTGCCTAGGTATC	18	20	55.6	55.0	56.2	57.6	0.0	11.3	4.221281	28739	29210	493-681	1.000000	58.2
rrn16-rrn23_p3	rrn16-rrn23	3	GGCTGGATCACCTCCTTT	AAGCATTTCGTCGCTTGCTA	18	20	55.6	45.0	56.2	58.6	0.0	14.1	4.399188	28739	29210	525-713	1.000000	56.8
rrn16-rrn23_p4	rrn16-rrn23	4	GGCTGGATCACCTCCTTT	CTCTGGGTGCCTAGGTATCC	18	20	55.6	60.0	56.2	58.7	0.0	21.7	4.494770	28739	29210	492-680	1.000000	56.0
rrn16-rrn23_p5	rrn16-rrn23	5	GGCTGGATCACCTCCTTT	TGGGTGCCTAGGTATCCAC	18	19	55.6	57.9	56.2	58.1	0.0	26.7	4.912761	28739	29210	489-677	1.000000	52.7
rrn23-rrn4.5_p1	rrn23-rrn4.5	1	CCACCTCAAGATGAGTGCTC	TGATAAACGGCTCGTCTTGC	20	20	55.0	50.0	58.0	58.6	6.6	0.7	0.665384	32042	32144	154-155	1.000000	82.9
rrn23-rrn4.5_p2	rrn23-rrn4.5	2	CCTCAAGATGAGTGCTCTCC	TGATAAACGGCTCGTCTTGC	20	20	55.0	50.0	57.4	58.6	13.0	0.7	1.244499	32042	32144	151-152	1.000000	78.5
rrn23-rrn4.5_p3	rrn23-rrn4.5	3	CCACCTCAAGATGAGTGCTC	ATAAACGGCTCGTCTTGCC	20	19	55.0	52.6	58.0	58.2	6.6	0.7	1.257704	32042	32144	152-153	1.000000	78.3
rrn23-rrn4.5_p4	rrn23-rrn4.5	4	CCACCTCAAGATGAGTGCTC	GATAAACGGCTCGTCTTGCC	20	20	55.0	55.0	58.0	59.3	6.6	0.7	1.370324	32042	32144	153-154	1.000000	77.4
rrn23-rrn4.5_p5	rrn23-rrn4.5	5	CACCTCAAGATGAGTGCTCT	TGATAAACGGCTCGTCTTGC	20	20	50.0	50.0	56.9	58.6	6.6	0.7	1.699122	32042	32144	153-154	1.000000	74.7
rrn4.5-rrn5_p1	rrn4.5-rrn5	1	TGAGGCATCCTAACAGACCA	GCCTAGGACACCAGAATA	20	18	50.0	50.0	58.0	52.5	0.0	14.8	7.591816	32248	32387	191-289	1.000000	51.0
rrn4.5-rrn5_p2	rrn4.5-rrn5	2	CAGCTGAGGCATCCTAACAG	GCCTAGGACACCAGAATA	20	18	55.0	50.0	58.0	52.5	8.4	14.8	7.592197	32248	32387	195-293	1.000000	51.0
rrn4.5-rrn5_p3	rrn4.5-rrn5	3	GCTGAGGCATCCTAACAGAC	GCCTAGGACACCAGAATA	20	18	55.0	50.0	58.1	52.5	0.0	14.8	7.596280	32248	32387	193-291	1.000000	51.0
rrn4.5-rrn5_p4	rrn4.5-rrn5	4	AGCTGAGGCATCCTAACAGA	GCCTAGGACACCAGAATA	20	18	50.0	50.0	58.1	52.5	0.0	14.8	7.671607	32248	32387	194-292	1.000000	51.0
rrn4.5-rrn5_p5	rrn4.5-rrn5	5	CTGAGGCATCCTAACAGACC	GCCTAGGACACCAGAATA	20	18	55.0	50.0	57.4	52.5	0.0	14.8	8.154250	32248	32387	192-290	1.000000	51.0
rrn5-ycf1_p1	rrn5-ycf1	1	CCACCTCAAGATGAGTGCTC	TGCTGAGATCACGCATTGAA	20	20	55.0	45.0	58.0	57.9	6.6	0.0	0.119035	32509	32951	1321-1608	1.000000	89.1
rrn5-ycf1_p2	rrn5-ycf1	2	CCACCTCAAGATGAGTGCTC	TTGAATGCTGAGATCACGCA	20	20	55.0	45.0	58.0	57.9	6.6	10.7	0.119035	32509	32951	1326-1613	1.000000	89.0
rrn5-ycf1_p3	rrn5-ycf1	3	ACCTGGGGCTGTAGTATGTT	TGCTGAGATCACGCATTGAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.130835	32509	32951	1571-1858	1.000000	87.7
rrn5-ycf1_p4	rrn5-ycf1	4	ACCTGGGGCTGTAGTATGTT	TTGAATGCTGAGATCACGCA	20	20	50.0	45.0	58.0	57.9	0.0	10.7	0.130835	32509	32951	1576-1863	1.000000	87.7
rrn5-ycf1_p5	rrn5-ycf1	5	TGGGGCTGTAGTATGTTCCA	TGCTGAGATCACGCATTGAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.137970	32509	32951	1568-1855	1.000000	87.7
ycf1-rps12_p1	ycf1-rps12	1	TCTCCCAGACATCGTAGAGA	GGTAGCCTGCTTAAGTTCCC	20	20	50.0	55.0	56.6	58.0	0.0	4.0	1.437202	37689	38713	1698-1785	1.000000	76.1
ycf1-rps12_p2	ycf1-rps12	2	TCTCCCAGACATCGTAGAGA	AGCCATACACTTCGCATGTT	20	20	50.0	45.0	56.6	57.9	0.0	0.0	1.511244	37689	38713	1654-1741	1.000000	75.7
ycf1-rps12_p3	ycf1-rps12	3	TCTCCCAGACATCGTAGAGA	ACGGCTTTCCACAGAATTGT	20	20	50.0	45.0	56.6	58.0	0.0	0.0	1.416127	37689	38713	2080-2170	0.750000	65.7
ycf1-rps12_p4	ycf1-rps12	4	TCTCCCAGACATCGTAGAGA	GGTTAGCCATACACTTCGCA	20	20	50.0	50.0	56.6	58.0	0.0	0.0	1.415926	37689	38713	1658-1679	0.750000	64.4
ycf1-rps12_p5	ycf1-rps12	5	TCTCCCAGACATCGTAGAGA	ACCGGGCAAATCCTTAACTC	20	20	50.0	50.0	56.6	57.9	0.0	6.3	1.521661	37689	38713	1499-1499	0.250000	37.8
trnL-CAA-rps16_p1	trnL-CAA-rps16	1	ATCGGCAATGGGTCTGAAAG	GGTCCGAGGCTATTGACAAA	20	20	50.0	50.0	58.2	57.6	0.0	11.2	0.641410	44310	44459	1054-1054	0.250000	44.9
trnL-CAA-rps16_p2	trnL-CAA-rps16	2	ATCGGCAATGGGTCTGAAAG	ATTGGTCCGAGGCTATTGAC	20	20	50.0	50.0	58.2	57.4	0.0	0.0	0.866884	44310	44459	1057-1057	0.250000	43.1
trnL-CAA-rps16_p3	trnL-CAA-rps16	3	CATCGGCAATGGGTCTGAAA	GGTCCGAGGCTATTGACAAA	20	20	50.0	50.0	58.5	57.6	0.0	11.2	0.933974	44310	44459	1055-1055	0.250000	42.5
trnL-CAA-rps16_p4	trnL-CAA-rps16	4	ATCGGCAATGGGTCTGAAAG	TGGTCCGAGGCTATTGACAA	20	20	50.0	50.0	58.2	58.7	0.0	11.2	0.972964	44310	44459	1055-1055	0.250000	42.2
trnL-CAA-rps16_p5	trnL-CAA-rps16	5	ATCGGCAATGGGTCTGAAAG	TTGGTCCGAGGCTATTGACA	20	20	50.0	50.0	58.2	58.7	0.0	11.2	0.972964	44310	44459	1056-1056	0.250000	42.2