primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.171606	85	320	685-1016	1.000000	80.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.177364	85	320	684-1015	1.000000	80.7
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.175967	85	320	699-1030	1.000000	80.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	85	320	698-1029	1.000000	80.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	85	320	700-1031	1.000000	80.6
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTGAAAAAGGGGCTCAACCT	20	20	50.0	45.0	58.2	57.5	20.3	0.0	0.638477	4180	5126	1050-1066	1.000000	80.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTGAAAAAGGGGCTCAACCT	20	20	50.0	45.0	58.2	57.5	20.3	0.0	0.638477	4180	5126	1049-1065	1.000000	80.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTAGCCGCACTTAAAAGCC	TTGAAAAAGGGGCTCAACCT	20	20	50.0	45.0	58.3	57.5	0.0	0.0	0.735894	4180	5126	1064-1080	1.000000	79.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	TTGAAAAAGGGGCTCAACCT	20	20	50.0	45.0	58.3	57.5	5.3	0.0	0.752863	4180	5126	1059-1075	1.000000	79.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AAAAGGGGCTCAACCTACAG	20	20	50.0	50.0	58.2	57.4	20.3	0.0	0.755068	4180	5126	1045-1061	1.000000	79.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	20	20	45.0	50.0	58.1	58.0	8.1	0.0	0.148076	6236	7479	1338-1375	1.000000	85.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6236	7479	1280-1317	1.000000	85.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAAATTGAAATGGGGCGTGG	20	20	55.0	50.0	58.4	58.0	2.9	0.0	0.378604	6236	7479	1352-1389	1.000000	83.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6236	7479	1294-1331	1.000000	83.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	57.2	58.0	0.0	0.0	0.796474	6236	7479	1330-1367	1.000000	80.6
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071561	8585	8708	224-237	1.000000	85.9
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	CTTTTGTTTGGCAAGCTGCT	TGGACTAAAGCGTCGGATTG	20	20	45.0	50.0	58.1	58.0	15.2	0.0	0.060551	8585	8708	726-739	1.000000	82.5
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.9	58.0	1.4	0.0	0.060677	8585	8708	721-734	1.000000	82.5
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	57.9	58.0	1.4	0.0	0.062065	8585	8708	722-735	1.000000	82.5
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	CTTTTGTTTGGCAAGCTGCT	GTGGACTAAAGCGTCGGATT	20	20	45.0	50.0	58.1	58.0	15.2	0.0	0.061939	8585	8708	727-740	1.000000	82.4
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CGGTGCTCTGACCAATTGAA	CTGGTTCAAATCCAGCTCGG	20	20	50.0	55.0	58.5	58.9	7.1	0.0	1.387412	31507	31607	155-155	1.000000	72.5
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	CGGTGCTCTGACCAATTGAA	TCTACGCTGGTTCAAATCCA	20	20	50.0	45.0	58.5	56.9	7.1	0.0	1.618630	31507	31607	161-161	1.000000	70.5
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	TGGTTCAAATCCAGCTCGG	20	19	50.0	52.6	58.5	57.8	7.1	0.0	1.725375	31507	31607	154-154	1.000000	69.8
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	CGGTGCTCTGACCAATTGAA	GTCTACGCTGGTTCAAATCCA	20	21	50.0	47.6	58.5	58.3	7.1	0.0	1.781973	31507	31607	162-162	1.000000	69.2
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CGGTGCTCTGACCAATTGAA	TGTCTACGCTGGTTCAAATCC	20	21	50.0	47.6	58.5	58.3	7.1	0.0	1.781973	31507	31607	163-163	1.000000	69.2
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CGAATTTACAGTCCGTCCCC	CTCTCTTTCAAGGAGGCAGC	20	20	55.0	55.0	58.4	58.3	0.0	0.0	0.614884	31692	31750	147-147	1.000000	76.7
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	ACGAATTTACAGTCCGTCCC	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.6	58.3	0.0	0.0	0.638561	31692	31750	148-148	1.000000	76.5
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	GCCAACGAATTTACAGTCCG	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.5	58.3	0.0	0.0	0.784075	31692	31750	152-152	1.000000	75.3
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	CGAATTTACAGTCCGTCCCC	GATAGTATGAGGGCGGTTGG	20	20	55.0	55.0	58.4	57.5	0.0	0.0	0.808862	31692	31750	203-203	1.000000	74.7
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	ACGAATTTACAGTCCGTCCC	GATAGTATGAGGGCGGTTGG	20	20	50.0	55.0	57.6	57.5	0.0	0.0	0.832539	31692	31750	204-204	1.000000	74.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	47430	48147	754-779	1.000000	82.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	47430	48147	786-811	1.000000	81.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.482748	47430	48147	805-830	1.000000	81.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	47430	48147	748-773	1.000000	81.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	47430	48147	786-811	1.000000	80.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	AATCGGGGCTAAAATACCGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.462872	51509	52678	1296-1305	1.000000	80.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAATACCGGCAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.497553	51509	52678	1292-1301	1.000000	80.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.605515	51509	52678	1313-1322	1.000000	79.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.6	57.8	0.0	0.0	0.766291	51509	52678	1305-1314	1.000000	78.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	AATCGGGGCTAAAATACCGG	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.7	57.5	0.0	0.0	0.806644	51509	52678	1295-1304	1.000000	77.8
accD-psaI_p1	accD-psaI	1	CCAGAAGGGCTTATTCGACC	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	0.0	26.9	0.072076	60118	60728	759-793	1.000000	84.5
accD-psaI_p2	accD-psaI	2	CCAGAAGGGCTTATTCGACC	AAGAAGCCATTGCAATTGCC	20	20	55.0	45.0	58.0	57.9	0.0	26.8	0.148610	60118	60728	765-799	1.000000	83.8
accD-psaI_p3	accD-psaI	3	CCAGAAGGGCTTATTCGACC	GCCGGAAATACTAGGCCTAC	20	20	55.0	55.0	58.0	57.5	0.0	8.9	0.497368	60118	60728	748-782	1.000000	81.1
accD-psaI_p4	accD-psaI	4	GACCTAATCGTACCGCGTAA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.2	58.0	4.9	26.9	0.782840	60118	60728	743-777	1.000000	78.8
accD-psaI_p5	accD-psaI	5	GACCTAATCGTACCGCGTAA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.2	57.9	4.9	26.8	0.859374	60118	60728	749-783	1.000000	78.2
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	CGCTCTGTAGGACTTGAACC	GTCACGGGTTCAAATCCTGT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.110121	67747	67957	311-353	1.000000	84.7
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	CGCTCTGTAGGACTTGAACC	CACGGGTTCAAATCCTGTCA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.111455	67747	67957	309-351	1.000000	84.7
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	CGCTCTGTAGGACTTGAACC	TGTCACGGGTTCAAATCCTG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.111455	67747	67957	312-354	1.000000	84.6
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	CGCTCTGTAGGACTTGAACC	GGGTACAAAATGTCACGGGT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.102085	67747	67957	322-364	1.000000	84.5
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	CGCTCTGTAGGACTTGAACC	TGGGTACAAAATGTCACGGG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.103429	67747	67957	323-365	1.000000	84.5
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	CTAAAGCCCCGAACCATAGC	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.505424	68032	68455	546-567	1.000000	77.5
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	ACCCGTGACATTTTGTACCC	GCTAAAGCCCCGAACCATAG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.505424	68032	68455	547-568	1.000000	77.5
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	CCCGTGACATTTTGTACCCA	CTAAAGCCCCGAACCATAGC	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.506768	68032	68455	545-566	1.000000	77.5
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CCCGTGACATTTTGTACCCA	GCTAAAGCCCCGAACCATAG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.506768	68032	68455	546-567	1.000000	77.5
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	ACAGGATTTGAACCCGTGAC	CTAAAGCCCCGAACCATAGC	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.513460	68032	68455	557-578	1.000000	77.4
psaJ-rpl33_p1	psaJ-rpl33	1	ATGCGTTGACATTCCCCTTT	CGAACAAACGACCAATCTGC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.126173	68591	69086	1261-1261	0.500000	59.0
psaJ-rpl33_p2	psaJ-rpl33	2	ATGCGTTGACATTCCCCTTT	GAACAAACGACCAATCTGCG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.126173	68591	69086	1260-1260	0.500000	59.0
psaJ-rpl33_p3	psaJ-rpl33	3	ACCCGTGACATTTTGTACCC	CGAACAAACGACCAATCTGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.149283	68591	69086	1843-1843	0.500000	58.8
psaJ-rpl33_p4	psaJ-rpl33	4	ACCCGTGACATTTTGTACCC	GAACAAACGACCAATCTGCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.149283	68591	69086	1842-1842	0.500000	58.8
psaJ-rpl33_p5	psaJ-rpl33	5	CCCGTGACATTTTGTACCCA	GAACAAACGACCAATCTGCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.150627	68591	69086	1841-1841	0.500000	58.8
rps18-rpl20_p1	rps18-rpl20	1	TGAAAGAACCGAGTCAACCG	TGGAAAGAATACACCGGAAA	20	20	50.0	40.0	58.1	54.6	0.0	9.5	3.564363	69753	69983	313-313	0.500000	31.5
rps18-rpl20_p2	rps18-rpl20	2	TGAAAGAACCGAGTCAACCG	TTGGAAAGAATACACCGGAA	20	20	50.0	40.0	58.1	54.6	0.0	9.5	3.564363	69753	69983	314-314	0.500000	31.5
rps18-rpl20_p3	rps18-rpl20	3	TGAAAGAACCGAGTCAACCG	TGGAAAGAATACACCGGAAAA	20	21	50.0	38.1	58.1	55.4	0.0	9.5	3.719329	69753	69983	313-313	0.500000	30.2
rps18-rpl20_p4	rps18-rpl20	4	TGAAAGAACCGAGTCAACCG	TTGGAAAGAATACACCGGAAA	20	21	50.0	38.1	58.1	55.4	0.0	9.5	3.719329	69753	69983	314-314	0.500000	30.2
rps18-rpl20_p5	rps18-rpl20	5	TGAAAGAACCGAGTCAACCG	TGGAAAGAATACACCGGAAAAA	20	22	50.0	36.4	58.1	56.2	0.0	9.5	3.951010	69753	69983	313-313	0.500000	28.4
rps11-rpl36_p1	rps11-rpl36	1	ACTTCCACTGTAGTGTCCGA	AGGTGGGATGGGGATTGTAA	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.014480	80768	80878	1310-1315	1.000000	82.0
rps11-rpl36_p2	rps11-rpl36	2	TTCCACTGTAGTGTCCGAGT	AGGTGGGATGGGGATTGTAA	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.014480	80768	80878	1308-1313	1.000000	82.0
rps11-rpl36_p3	rps11-rpl36	3	ACTTCCACTGTAGTGTCCGA	GCTTCCAAATGGCATGTTCC	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.023214	80768	80878	1039-1044	1.000000	82.0
rps11-rpl36_p4	rps11-rpl36	4	TTCCACTGTAGTGTCCGAGT	GCTTCCAAATGGCATGTTCC	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.023214	80768	80878	1037-1042	1.000000	82.0
rps11-rpl36_p5	rps11-rpl36	5	TTCCACTGTAGTGTCCGAGT	TCAAGGGAGCTTTACCTGGA	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.047742	80768	80878	1101-1106	1.000000	81.7
rpl16-rps3_p1	rpl16-rps3	1	AAACCAACGAGTCACACACT	CCCTACAAACCATTCGAGCT	20	20	45.0	50.0	57.6	57.6	0.0	0.0	0.799841	83832	83999	303-304	1.000000	76.2
rpl16-rps3_p2	rpl16-rps3	2	TGATACTACGGGGTTGGTCT	CCCTACAAACCATTCGAGCT	20	20	50.0	50.0	57.5	57.6	0.0	0.0	0.940885	83832	83999	348-352	1.000000	75.4
rpl16-rps3_p3	rpl16-rps3	3	AAACCAACGAGTCACACACT	GGGTTCCCCTACAAACCATT	20	20	45.0	50.0	57.6	57.4	0.0	6.6	1.012576	83832	83999	309-310	1.000000	74.5
rpl16-rps3_p4	rpl16-rps3	4	AAACCAACGAGTCACACACT	CCCCTACAAACCATTCGAGC	20	20	45.0	55.0	57.6	58.6	0.0	0.0	1.017063	83832	83999	304-305	1.000000	74.5
rpl16-rps3_p5	rpl16-rps3	5	TGATACTACGGGGTTGGTCT	GGGTTCCCCTACAAACCATT	20	20	50.0	50.0	57.5	57.4	0.0	6.6	1.153619	83832	83999	354-358	1.000000	73.7
rps15-ycf1_p1	rps15-ycf1	1	CAGACCCTCTGTTTTCTTCGT	GGAAGTCGTTTCAGCATGTT	21	20	47.6	45.0	57.9	56.6	0.0	0.0	2.464447	123002	123386	509-513	1.000000	63.1
rps15-ycf1_p2	rps15-ycf1	2	AGACCCTCTGTTTTCTTCGT	GGAAGTCGTTTCAGCATGTT	20	20	45.0	45.0	56.4	56.6	0.0	0.0	2.947667	123002	123386	508-512	1.000000	59.2
rps15-ycf1_p3	rps15-ycf1	3	CAGACCCTCTGTTTTCTTCGT	AGTCGTTTCAGCATGTTAAGGA	21	22	47.6	40.9	57.9	58.0	0.0	0.0	3.116166	123002	123386	506-510	1.000000	57.9
rps15-ycf1_p4	rps15-ycf1	4	AGACCCTCTGTTTTCTTCGTT	GGAAGTCGTTTCAGCATGTT	21	20	42.9	45.0	57.2	56.6	0.0	0.0	3.191311	123002	123386	508-512	1.000000	57.3
rps15-ycf1_p5	rps15-ycf1	5	CAGACCCTCTGTTTTCTTCG	GGAAGTCGTTTCAGCATGTT	20	20	50.0	45.0	56.1	56.6	0.0	0.0	3.257690	123002	123386	509-513	1.000000	56.8
ycf1_p1	ycf1	1	CGTTTCATCGATTTTGTTCGGA	AGGAACTTTACAAACGGAAGA	22	21	40.9	38.1	57.9	55.1	0.0	0.0	5.972052	128065	128184	166-166	1.000000	43.9
ycf1_p2	ycf1	2	CGTTTCATCGATTTTGTTCGGA	AGGAACTTTACAAACGGAAGAA	22	22	40.9	36.4	57.9	55.9	0.0	0.0	6.190845	128065	128184	166-166	1.000000	43.9
ycf1_p3	ycf1	3	TCGTTTCATCGATTTTGTTCGG	AGGAACTTTACAAACGGAAGA	22	21	40.9	38.1	57.9	55.1	0.0	0.0	5.972052	128065	128184	167-167	1.000000	43.8
ycf1_p4	ycf1	4	TCGTTTCATCGATTTTGTTCGG	AGGAACTTTACAAACGGAAGAA	22	22	40.9	36.4	57.9	55.9	0.0	0.0	6.190845	128065	128184	167-167	1.000000	43.8
ycf1_p5	ycf1	5	CGTTTCATCGATTTTGTTCGGA	ACCATCACCCATTCTTACTAAGA	22	23	40.9	39.1	57.9	56.7	0.0	0.0	6.359133	128065	128184	196-196	1.000000	43.3