primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.0	57.3	0.0	24.6	0.714678	8977	9837	973-1279	1.000000	78.0
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.0	57.3	0.0	24.6	0.716066	8977	9837	972-1278	1.000000	78.0
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.2	57.3	0.0	24.6	0.861444	8977	9837	1027-1333	1.000000	76.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	TGGAAACGGAAAGAGAGGGA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.3	57.3	0.0	24.6	0.985551	8977	9837	1032-1338	1.000000	75.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	20	21	50.0	47.6	58.0	58.0	0.0	7.0	1.031212	8977	9837	941-1247	1.000000	75.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTGGCCCTTTACTAATCGGG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.453400	54726	55350	668-773	1.000000	81.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTGGCCCTTTACTAATCGGG	AAATGCACGTTGGGTCTGTA	20	20	55.0	45.0	57.7	57.7	0.0	0.0	0.520122	54726	55350	736-841	1.000000	80.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTGGCCCTTTACTAATCGGG	AATGCACGTTGGGTCTGTAA	20	20	55.0	45.0	57.7	57.7	0.0	0.0	0.520122	54726	55350	735-840	1.000000	80.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CTGGCCCTTTACTAATCGGG	ATGCACGTTGGGTCTGTAAA	20	20	55.0	45.0	57.7	57.7	0.0	0.0	0.520122	54726	55350	734-839	1.000000	80.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTGGCCCTTTACTAATCGGG	ACGTTGGGTCTGTAAAGCAA	20	20	55.0	45.0	57.7	57.7	0.0	0.0	0.591433	54726	55350	730-835	1.000000	80.2
rbcL-accD_p1	rbcL-accD	1	AGTCCTGAATTAGCTGCTGC	TGGGTCTTCACTTACACTGG	20	20	50.0	50.0	58.0	56.8	13.4	0.0	1.257119	60618	61596	1135-1174	1.000000	74.4
rbcL-accD_p2	rbcL-accD	2	AGTCCTGAATTAGCTGCTGC	GGGTCTTCACTTACACTGGT	20	20	50.0	50.0	58.0	56.8	13.4	0.0	1.259338	60618	61596	1134-1173	1.000000	74.4
rbcL-accD_p3	rbcL-accD	3	GAGGCTAGCAAATGGAGTCC	TGGGTCTTCACTTACACTGG	20	20	55.0	50.0	58.0	56.8	18.2	0.0	1.261173	60618	61596	1150-1189	1.000000	74.4
rbcL-accD_p4	rbcL-accD	4	GAGGCTAGCAAATGGAGTCC	GGGTCTTCACTTACACTGGT	20	20	55.0	50.0	58.0	56.8	18.2	0.0	1.263391	60618	61596	1149-1188	1.000000	74.3
rbcL-accD_p5	rbcL-accD	5	TTCGTGAGGCTAGCAAATGG	TGGGTCTTCACTTACACTGG	20	20	50.0	50.0	58.3	56.8	18.2	0.0	1.483751	60618	61596	1155-1194	1.000000	72.6
accD-psaI_p1	accD-psaI	1	GCTCCATGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.3	58.0	0.0	26.9	1.731433	63193	63781	597-696	1.000000	72.3
accD-psaI_p2	accD-psaI	2	GCTCCATGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	56.3	57.9	0.0	26.8	1.807967	63193	63781	603-702	1.000000	71.6
accD-psaI_p3	accD-psaI	3	GCTCCATGCTTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	57.1	58.0	0.0	26.9	1.976845	63193	63781	597-696	1.000000	70.3
accD-psaI_p4	accD-psaI	4	GCTCCATGCTTTCTTTCCTTT	AAGAAGCCATTGCAATTGCC	21	20	42.9	45.0	57.1	57.9	0.0	26.8	2.053379	63193	63781	603-702	1.000000	69.7
accD-psaI_p5	accD-psaI	5	GTGAGTTATTTCAGCTCCATGC	CCATTGCAATTGCCGGAAAT	22	20	45.5	45.0	57.7	58.0	0.0	26.9	2.311381	63193	63781	610-709	1.000000	67.6
psbE-psbF_p1	psbE-psbF	1	AACTGGCCGTTTTGATCCTT	GAACAGCCAACCATCGTACT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.099386	73731	73739	116-116	1.000000	79.9
psbE-psbF_p2	psbE-psbF	2	ACTGGCCGTTTTGATCCTTT	GAACAGCCAACCATCGTACT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.099386	73731	73739	115-115	1.000000	79.9
psbE-psbF_p3	psbE-psbF	3	AACTGGCCGTTTTGATCCTT	AACAGCCAACCATCGTACTG	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.196213	73731	73739	115-115	1.000000	79.1
psbE-psbF_p4	psbE-psbF	4	ACTGGCCGTTTTGATCCTTT	AACAGCCAACCATCGTACTG	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.196213	73731	73739	114-114	1.000000	79.1
psbE-psbF_p5	psbE-psbF	5	ACTGGCCGTTTTGATCCTTT	TGAACAGCCAACCATCGTAC	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.201290	73731	73739	116-116	1.000000	79.1
psbJ-clpP_p1	psbJ-clpP	1	AACTGGCCGTTTTGATCCTT	TATCGACTCAACTTGCTGGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.014769	74253	75163	2051-2051	0.500000	59.9
psbJ-clpP_p2	psbJ-clpP	2	ACTGGCCGTTTTGATCCTTT	TATCGACTCAACTTGCTGGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.014769	74253	75163	2050-2050	0.500000	59.9
psbJ-clpP_p3	psbJ-clpP	3	AACTGGCCGTTTTGATCCTT	CCTGGGGAAGAAGATGCATC	20	20	45.0	55.0	58.0	58.0	0.0	19.4	0.026169	74253	75163	2142-2142	0.500000	59.8
psbJ-clpP_p4	psbJ-clpP	4	ACTGGCCGTTTTGATCCTTT	CCTGGGGAAGAAGATGCATC	20	20	45.0	55.0	58.0	58.0	0.0	19.4	0.026169	74253	75163	2141-2141	0.500000	59.8
psbJ-clpP_p5	psbJ-clpP	5	ACTGGCCGTTTTGATCCTTT	TGCTGTATCCGAACACATGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.027388	74253	75163	1677-1677	0.500000	59.8
clpP_p1	clpP	1	ACCCCGTTTTCCAATTTCGA	CAGAACGAGAACGGGCTTAA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.090481	75164	75853	981-981	0.500000	59.3
clpP_p2	clpP	2	ACCCCGTTTTCCAATTTCGA	TAAGAATGCGCAATGGTGGA	20	20	45.0	45.0	58.0	57.9	0.0	17.1	0.137226	75164	75853	1368-1368	0.500000	58.9
clpP_p3	clpP	3	ACCCCGTTTTCCAATTTCGA	GTTGTTCATCACTCAGGGGG	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.189549	75164	75853	1298-1298	0.500000	58.5
clpP_p4	clpP	4	ACCCCGTTTTCCAATTTCGA	GTAAGAATGCGCAATGGTGG	20	20	45.0	50.0	58.0	57.8	0.0	17.1	0.209240	75164	75853	1369-1369	0.500000	58.3
clpP_p5	clpP	5	ACCCCGTTTTCCAATTTCGA	ACCAAGGCAAACCCATGAAA	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.216001	75164	75853	1577-1577	0.500000	58.3
clpP-psbB_p1	clpP-psbB	1	GGTCGGATCCTCCATACTGA	ATCAAAACCTTCCACACCCC	20	20	55.0	50.0	58.0	58.0	10.8	0.0	0.012883	75854	76586	1486-1486	0.500000	59.9
clpP-psbB_p2	clpP-psbB	2	GCCAGCAAGTTGAGTCGATA	ATCAAAACCTTCCACACCCC	20	20	50.0	50.0	58.0	58.0	7.2	0.0	0.019826	75854	76586	1453-1453	0.500000	59.8
clpP-psbB_p3	clpP-psbB	3	GGTCGGATCCTCCATACTGA	ACACCCATATTCCAGGACCA	20	20	55.0	50.0	58.0	58.0	10.8	0.0	0.023392	75854	76586	1418-1418	0.500000	59.8
clpP-psbB_p4	clpP-psbB	4	GGTCGGATCCTCCATACTGA	ATACTCCAACCACCCCATGA	20	20	55.0	50.0	58.0	58.0	10.8	0.0	0.023392	75854	76586	1152-1152	0.500000	59.8
clpP-psbB_p5	clpP-psbB	5	GGTCGGATCCTCCATACTGA	TGATACTCCAACCACCCCAT	20	20	55.0	50.0	58.0	58.0	10.8	0.0	0.023392	75854	76586	1154-1154	0.500000	59.8
rps11-rpl36_p1	rps11-rpl36	1	TACGCGCATTCTTACGTGAA	ACCCCGGAACAAAAATTCGA	20	20	45.0	45.0	58.0	58.0	5.7	0.7	0.036037	83619	83913	644-671	1.000000	82.8
rps11-rpl36_p2	rps11-rpl36	2	TTTTAGGGGGCCTACATCCA	ACCCCGGAACAAAAATTCGA	20	20	50.0	45.0	58.0	58.0	0.8	0.7	0.027395	83619	83913	977-1004	1.000000	82.4
rps11-rpl36_p3	rps11-rpl36	3	ACTTCCACTGTAGTGTCCGA	ACCCCGGAACAAAAATTCGA	20	20	50.0	45.0	58.0	58.0	10.8	0.7	0.024813	83619	83913	1117-1144	1.000000	82.3
rps11-rpl36_p4	rps11-rpl36	4	TTCCACTGTAGTGTCCGAGT	ACCCCGGAACAAAAATTCGA	20	20	50.0	45.0	58.0	58.0	10.8	0.7	0.024813	83619	83913	1115-1142	1.000000	82.3
rps11-rpl36_p5	rps11-rpl36	5	GAAATCACCTGACCCCGTAC	ACCCCGGAACAAAAATTCGA	20	20	55.0	45.0	58.0	58.0	0.0	0.7	0.035882	83619	83913	729-729	0.500000	59.7
rpl36_p1	rpl36	1	TTTTTGTGCATCCGGTCAGC	AGATTCGGAATTCAGTTAAGGA	20	22	50.0	36.4	59.7	54.9	0.0	7.9	6.782533	83914	84027	202-203	1.000000	42.4
rpl36_p2	rpl36	2	TTTTTGTGCATCCGGTCAGC	AGATTCGGAATTCAGTTAAGGAA	20	23	50.0	34.8	59.7	55.7	0.0	7.9	7.021403	83914	84027	202-203	1.000000	42.4
rpl36_p3	rpl36	3	TTTTGTGCATCCGGTCAGC	AGATTCGGAATTCAGTTAAGGA	19	22	52.6	36.4	59.0	54.9	0.0	7.9	7.140161	83914	84027	201-202	1.000000	42.4
rpl36_p4	rpl36	4	TTTTTGTGCATCCGGTCAGC	GATTCGGAATTCAGTTAAGGA	20	21	50.0	38.1	59.7	53.3	0.0	7.9	7.337043	83914	84027	201-202	1.000000	42.4
rpl36_p5	rpl36	5	TTTTTGTGCATCCGGTCAGC	AGATTCGGAATTCAGTTAAGG	20	21	50.0	38.1	59.7	53.3	0.0	7.9	7.353946	83914	84027	202-203	1.000000	42.4
ycf1_p1	ycf1	1	AGGGACGATGCTTATACGGA	GGAACGCCATTATAGGTGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047085	116416	116535	614-620	1.000000	83.5
ycf1_p2	ycf1	2	AGGGACGATGCTTATACGGA	TGGAACGCCATTATAGGTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047085	116416	116535	615-621	1.000000	83.5
ycf1_p3	ycf1	3	AGGGACGATGCTTATACGGA	TCTGTGCCAAGGTTTCAGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041560	116416	116535	705-711	1.000000	83.2
ycf1_p4	ycf1	4	AGGGACGATGCTTATACGGA	TGCCAAGGTTTCAGACGAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.054440	116416	116535	701-707	1.000000	83.1
ycf1_p5	ycf1	5	AGGGACGATGCTTATACGGA	CGTTTAGCCAGTCTTTCGGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.083426	116416	116535	652-658	1.000000	83.1
ndhI-ndhG_p1	ndhI-ndhG	1	AATCAAATTGCTTTGGGCCG	CAAGACCCGATCCCAGAAAG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.127518	124775	125393	733-752	1.000000	82.4
ndhI-ndhG_p2	ndhI-ndhG	2	AATCAAATTGCTTTGGGCCG	AAGACCCGATCCCAGAAAGA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.145058	124775	125393	732-751	1.000000	82.3
ndhI-ndhG_p3	ndhI-ndhG	3	AATCAAATTGCTTTGGGCCG	TCCCAACGAAAAGGCAGAAA	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.157730	124775	125393	795-814	1.000000	81.9
ndhI-ndhG_p4	ndhI-ndhG	4	AATCAAATTGCTTTGGGCCG	AGACCCGATCCCAGAAAGAT	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.280196	124775	125393	731-750	1.000000	81.2
ndhI-ndhG_p5	ndhI-ndhG	5	TTTGGGCCGTTTACCAATGT	CAAGACCCGATCCCAGAAAG	20	20	45.0	55.0	58.3	58.0	0.0	0.0	0.323966	124775	125393	722-741	1.000000	80.9
trnL-UAG-ndhF_p1	trnL-UAG-ndhF	1	AGCGTGTCTACCGATTTCAC	AAAATATGCAGGAGGTGGCC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.227328	130129	130559	566-566	0.500000	58.2
trnL-UAG-ndhF_p2	trnL-UAG-ndhF	2	CAGCGTGTCTACCGATTTCA	AAAATATGCAGGAGGTGGCC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.228698	130129	130559	567-567	0.500000	58.2
trnL-UAG-ndhF_p3	trnL-UAG-ndhF	3	TATCATGCCCTTTTCGGACG	AAAATATGCAGGAGGTGGCC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.271393	130129	130559	853-853	0.500000	57.8
trnL-UAG-ndhF_p4	trnL-UAG-ndhF	4	GCTGCATAGCCCAATACCAT	AAAATATGCAGGAGGTGGCC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.304357	130129	130559	1167-1167	0.500000	57.6
trnL-UAG-ndhF_p5	trnL-UAG-ndhF	5	GAAGTAGCAAAGCCTTGGGT	AAAATATGCAGGAGGTGGCC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.308440	130129	130559	1065-1065	0.500000	57.5
ndhF-rpl32_p1	ndhF-rpl32	1	GCAGCCCCACTCCTATTAAC	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.039126	132807	133717	1617-1674	1.000000	86.8
ndhF-rpl32_p2	ndhF-rpl32	2	GCAGCCCCACTCCTATTAAC	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.040497	132807	133717	1618-1675	1.000000	86.8
ndhF-rpl32_p3	ndhF-rpl32	3	TAGGGCGTGTGAACCAAAAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.062062	132807	133717	2046-2103	1.000000	85.4
ndhF-rpl32_p4	ndhF-rpl32	4	TAGGGCGTGTGAACCAAAAA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.063433	132807	133717	2047-2104	1.000000	85.4
ndhF-rpl32_p5	ndhF-rpl32	5	AGCAACTGAACCGCCAAATA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.103543	132807	133717	2255-2312	1.000000	84.6
rpl32_p1	rpl32	1	GTCTTTCACATCCACCTATAGCA	AGCGTGTCTACCGATTTCAC	23	20	43.5	50.0	58.0	58.0	0.0	0.0	3.031363	133718	133885	661-732	1.000000	63.0
rpl32_p2	rpl32	2	TGTCTTTCACATCCACCTATAGC	AGCGTGTCTACCGATTTCAC	23	20	43.5	50.0	58.0	58.0	0.0	0.0	3.031363	133718	133885	662-733	1.000000	63.0
rpl32_p3	rpl32	3	GTCTTTCACATCCACCTATAGCA	CAGCGTGTCTACCGATTTCA	23	20	43.5	50.0	58.0	58.0	0.0	0.0	3.032734	133718	133885	662-733	1.000000	62.9
rpl32_p4	rpl32	4	TGTCTTTCACATCCACCTATAGC	CAGCGTGTCTACCGATTTCA	23	20	43.5	50.0	58.0	58.0	0.0	0.0	3.032734	133718	133885	663-734	1.000000	62.9
rpl32_p5	rpl32	5	AGCAGGATAAAGATAAGGGTTGG	AGCGTGTCTACCGATTTCAC	23	20	43.5	50.0	57.9	58.0	0.0	0.0	3.061808	133718	133885	951-1013	1.000000	62.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CCATTTGGAAGGAGAAGGGG	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.157803	133886	134372	632-632	0.500000	58.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CCATTTGGAAGGAGAAGGGG	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.159174	133886	134372	633-633	0.500000	58.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CCATTTGGAAGGAGAAGGGG	TATCATGCCCTTTTCGGACG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.201869	133886	134372	919-919	0.500000	58.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CCATTTGGAAGGAGAAGGGG	GCTGCATAGCCCAATACCAT	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.234832	133886	134372	1233-1233	0.500000	58.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CCATTTGGAAGGAGAAGGGG	GAAGTAGCAAAGCCTTGGGT	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.238916	133886	134372	1131-1131	0.500000	58.1
ndhG-ndhI_p1	ndhG-ndhI	1	CAAGACCCGATCCCAGAAAG	AATCAAATTGCTTTGGGCCG	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.127518	139108	139726	733-752	1.000000	82.4
ndhG-ndhI_p2	ndhG-ndhI	2	AAGACCCGATCCCAGAAAGA	AATCAAATTGCTTTGGGCCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.145058	139108	139726	732-751	1.000000	82.3
ndhG-ndhI_p3	ndhG-ndhI	3	TCCCAACGAAAAGGCAGAAA	AATCAAATTGCTTTGGGCCG	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.157730	139108	139726	795-814	1.000000	81.9
ndhG-ndhI_p4	ndhG-ndhI	4	AGACCCGATCCCAGAAAGAT	AATCAAATTGCTTTGGGCCG	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.280196	139108	139726	731-750	1.000000	81.2
ndhG-ndhI_p5	ndhG-ndhI	5	CAAGACCCGATCCCAGAAAG	TTTGGGCCGTTTACCAATGT	20	20	55.0	45.0	58.0	58.3	0.0	0.0	0.323966	139108	139726	722-741	1.000000	80.9