primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	trnK-UUU-rbcL	1	TGGAGTCGCACTTAAAAGCC	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	58.5	58.0	0.0	13.4	0.516122	4491	5528	1155-1161	1.000000	77.5
trnK-UUU-rbcL_p2	trnK-UUU-rbcL	2	AACCCTACCGATGGTATGGA	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	57.5	58.0	20.0	13.4	0.517944	4491	5528	1208-1214	1.000000	77.4
trnK-UUU-rbcL_p3	trnK-UUU-rbcL	3	AGTCGTGGTCTTACAAACCC	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	57.5	58.0	0.0	13.4	0.574042	4491	5528	1223-1229	1.000000	77.0
trnK-UUU-rbcL_p4	trnK-UUU-rbcL	4	GTCGTGGTCTTACAAACCCT	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	57.5	58.0	0.0	13.4	0.574042	4491	5528	1222-1228	1.000000	77.0
trnK-UUU-rbcL_p5	trnK-UUU-rbcL	5	AGTCGCACTTAAAAGCCGAG	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	58.6	58.0	6.4	13.4	0.606067	4491	5528	1152-1158	1.000000	76.8
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	ACCAATTTCGCCATATCCCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137364	14505	16062	1592-1608	1.000000	82.3
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	14505	16062	1600-1616	1.000000	81.7
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	ACCAATTTCGCCATATCCCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.0	57.8	0.0	15.5	0.245117	14505	16062	1624-1640	1.000000	81.3
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.1	57.8	0.0	15.5	0.309926	14505	16062	1632-1648	1.000000	80.8
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.2	57.9	10.2	0.0	0.372548	14505	16062	1606-1622	1.000000	80.3
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	CGAATCGATCCCTCCGTAAC	CAGTGTGGTAGAGTAACGCC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.055509	29967	31487	1609-1668	1.000000	83.6
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	AAAACGAATCGATCCCTCCG	CAGTGTGGTAGAGTAACGCC	20	20	50.0	55.0	58.1	58.0	10.5	0.0	0.071898	29967	31487	1613-1672	1.000000	83.5
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	CGAATCGATCCCTCCGTAAC	ACTCCTATTGGCTGGTTCCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.075091	29967	31487	1665-1724	1.000000	83.3
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	AAAACGAATCGATCCCTCCG	ACTCCTATTGGCTGGTTCCT	20	20	50.0	50.0	58.1	58.0	10.5	0.0	0.091480	29967	31487	1669-1728	1.000000	83.2
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	CGAATCGATCCCTCCGTAAC	GTGGTAGAGTAACGCCATGG	20	20	55.0	55.0	58.0	58.1	0.0	7.6	0.111746	29967	31487	1605-1664	1.000000	83.2
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.8	58.5	0.0	7.1	0.716274	32186	32623	508-546	1.000000	75.9
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	GGCAATATGTCTACGCTGGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.8	58.5	0.0	7.1	0.717825	32186	32623	507-545	1.000000	75.9
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	32186	32623	529-567	1.000000	75.0
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	32186	32623	528-566	1.000000	74.8
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	GATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.889785	32186	32623	562-600	1.000000	74.4
atpH-atpF_p1	atpH-atpF	1	CCGGAAGCAGAGGGAAAAAT	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	58.2	57.9	0.0	13.0	0.274437	49994	50659	833-834	1.000000	78.4
atpH-atpF_p2	atpH-atpF	2	CCGGAAGCAGAGGGAAAAAT	CGGATGGCCAGTAACTCAAG	20	20	50.0	55.0	58.2	58.3	0.0	13.0	0.503924	49994	50659	835-836	1.000000	76.5
atpH-atpF_p3	atpH-atpF	3	CCGGAAGCAGAGGGAAAAAT	TGGCCAGTAACTCAAGCAAA	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.517241	49994	50659	831-832	1.000000	76.4
atpH-atpF_p4	atpH-atpF	4	TGGGCTGGTTGTAGCATTAG	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	57.6	57.9	0.0	13.0	0.532228	49994	50659	762-763	1.000000	76.4
atpH-atpF_p5	atpH-atpF	5	AGCAGAGGGAAAAATACGGG	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	57.6	57.9	0.0	13.0	0.533919	49994	50659	828-829	1.000000	76.3
trnR-UCU-trnG-UCC_p1	trnR-UCU-trnG-UCC	1	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	21	20	47.6	50.0	56.9	58.0	0.0	0.0	2.081443	53776	54086	391-398	1.000000	65.4
trnR-UCU-trnG-UCC_p2	trnR-UCU-trnG-UCC	2	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	21	20	47.6	55.0	56.9	58.1	0.0	0.0	2.110280	53776	54086	394-401	1.000000	65.1
trnR-UCU-trnG-UCC_p3	trnR-UCU-trnG-UCC	3	AGGTTTAGAAGACCTCTGTCCT	AGCCTTCCAAGCTAACGATG	22	20	45.5	50.0	57.9	58.0	0.0	0.0	2.164771	53776	54086	397-404	1.000000	64.7
trnR-UCU-trnG-UCC_p4	trnR-UCU-trnG-UCC	4	AGGTTTAGAAGACCTCTGTCCT	CCTAGCCTTCCAAGCTAACG	22	20	45.5	55.0	57.9	58.1	0.0	0.0	2.193608	53776	54086	400-407	1.000000	64.4
trnR-UCU-trnG-UCC_p5	trnR-UCU-trnG-UCC	5	AGAAGACCTCTGTCCTATCCA	CCCTAGCCTTCCAAGCTAAC	21	20	47.6	55.0	56.9	57.7	0.0	0.0	2.385293	53776	54086	395-402	1.000000	62.9
trnG-UCC-trnS-GCU_p1	trnG-UCC-trnS-GCU	1	TGAATCAAGCAAGGGATCCC	CAATCCGACGCTTTAGTCCA	20	20	50.0	50.0	57.6	58.0	24.6	0.0	0.446819	54860	55675	928-937	1.000000	79.9
trnG-UCC-trnS-GCU_p2	trnG-UCC-trnS-GCU	2	TGAATCAAGCAAGGGATCCC	AATCCGACGCTTTAGTCCAC	20	20	50.0	50.0	57.6	58.0	24.6	0.0	0.448207	54860	55675	927-936	1.000000	79.9
trnG-UCC-trnS-GCU_p3	trnG-UCC-trnS-GCU	3	TGAATCAAGCAAGGGATCCC	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.6	58.2	24.6	0.0	0.593585	54860	55675	981-990	1.000000	78.6
trnG-UCC-trnS-GCU_p4	trnG-UCC-trnS-GCU	4	TGAATCAAGCAAGGGATCCC	AGCAATCCGACGCTTTAGTC	20	20	50.0	50.0	57.6	58.4	24.6	0.0	0.800659	54860	55675	930-939	1.000000	77.1
trnG-UCC-trnS-GCU_p5	trnG-UCC-trnS-GCU	5	TGAATCAAGCAAGGGATCCC	CGACGCTTTAGTCCACTCAG	20	20	50.0	55.0	57.6	58.4	24.6	0.0	0.823376	54860	55675	923-932	1.000000	76.9
trnQ-UUG-accD_p1	trnQ-UUG-accD	1	GAGGTTCGAATCCTTCCGTC	CCGCCCTTACCACAAATGTA	20	20	55.0	50.0	58.1	57.9	16.9	0.0	0.192928	57417	59732	2865-2910	1.000000	81.9
trnQ-UUG-accD_p2	trnQ-UUG-accD	2	GAGGTTCGAATCCTTCCGTC	ATTTCCGCCCTTACCACAAA	20	20	55.0	45.0	58.1	57.7	16.9	0.0	0.372174	57417	59732	2869-2914	1.000000	80.5
trnQ-UUG-accD_p3	trnQ-UUG-accD	3	GAGGTTCGAATCCTTCCGTC	TTTCCGCCCTTACCACAAAT	20	20	55.0	45.0	58.1	57.7	16.9	0.0	0.372174	57417	59732	2868-2913	1.000000	80.5
trnQ-UUG-accD_p4	trnQ-UUG-accD	4	GAGGTTCGAATCCTTCCGTC	ATTCCGGATGTTCCTGACAC	20	20	55.0	50.0	58.1	57.6	16.9	4.1	0.479353	57417	59732	2521-2566	1.000000	80.0
trnQ-UUG-accD_p5	trnQ-UUG-accD	5	GAGGTTCGAATCCTTCCGTC	TTCCGCCCTTACCACAAATG	20	20	55.0	50.0	58.1	58.5	16.9	0.0	0.535834	57417	59732	2867-2912	1.000000	79.2
accD-psaI_p1	accD-psaI	1	CCATGCTTTCTTTCCTTTGCC	CCATTGCAATTGCCGGAAAT	21	20	47.6	45.0	58.3	58.0	0.0	26.9	1.335076	61236	61765	643-647	1.000000	70.9
accD-psaI_p2	accD-psaI	2	TGCTTTCTTTCCTTTGCCTCA	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	58.3	58.0	0.0	26.9	1.361838	61236	61765	640-644	1.000000	70.7
accD-psaI_p3	accD-psaI	3	CCATGCTTTCTTTCCTTTGCC	AAGAAGCCATTGCAATTGCC	21	20	47.6	45.0	58.3	57.9	0.0	26.8	1.411610	61236	61765	649-653	1.000000	70.3
accD-psaI_p4	accD-psaI	4	TGCTTTCTTTCCTTTGCCTCA	AAGAAGCCATTGCAATTGCC	21	20	42.9	45.0	58.3	57.9	0.0	26.8	1.438372	61236	61765	646-650	1.000000	70.1
accD-psaI_p5	accD-psaI	5	GCTTTCTTTCCTTTGCCTCA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.5	58.0	0.0	26.9	1.501481	61236	61765	639-643	1.000000	69.6
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	CGCTAAAACCCCGAACCATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.126758	68923	69375	577-583	1.000000	81.7
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	CGCTAAAACCCCGAACCATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.128102	68923	69375	576-582	1.000000	81.7
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	CGCTAAAACCCCGAACCATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.134794	68923	69375	588-594	1.000000	81.6
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	CGCTAAAACCCCGAACCATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.136127	68923	69375	587-593	1.000000	81.6
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	CGCTAAAACCCCGAACCATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.136127	68923	69375	590-596	1.000000	81.6
rps18_p1	rps18	1	TTGTGTGTCACCCTTCCAAG	GAGATTTTCGCGGTTTGTGG	20	20	50.0	50.0	57.9	58.0	0.0	6.1	0.087588	70483	70797	1046-1064	1.000000	81.7
rps18_p2	rps18	2	CTTGTGTGTCACCCTTCCAA	GAGATTTTCGCGGTTTGTGG	20	20	50.0	50.0	57.9	58.0	0.0	6.1	0.087588	70483	70797	1047-1065	1.000000	81.6
rps18_p3	rps18	3	GGTTCATGGCCAAGGGTAAA	GAGATTTTCGCGGTTTGTGG	20	20	50.0	50.0	58.1	58.0	11.0	6.1	0.104618	70483	70797	1266-1284	1.000000	81.2
rps18_p4	rps18	4	AAAGGAGGTTCATGGCCAAG	GAGATTTTCGCGGTTTGTGG	20	20	50.0	50.0	58.1	58.0	11.0	6.1	0.107385	70483	70797	1272-1290	1.000000	81.2
rps18_p5	rps18	5	TGGAAATGGAATGCTAGGGC	GAGATTTTCGCGGTTTGTGG	20	20	50.0	50.0	57.9	58.0	0.0	6.1	0.108031	70483	70797	1512-1530	1.000000	80.9
clpP-psbB_p1	clpP-psbB	1	GAACTCGAAACGGCACTTTG	ACCAAAACAAGCTACCCCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023341	73628	74716	1443-1582	1.000000	85.8
clpP-psbB_p2	clpP-psbB	2	GAACTCGAAGTGCCATGCTA	ACCAAAACAAGCTACCCCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.014769	73628	74716	1879-1999	1.000000	85.7
clpP-psbB_p3	clpP-psbB	3	CACTGTTGACTTCTTGGCCT	ACCAAAACAAGCTACCCCTG	20	20	50.0	50.0	58.0	58.0	3.3	0.0	0.031752	73628	74716	1530-1669	1.000000	85.6
clpP-psbB_p4	clpP-psbB	4	GAACTCGAAGTGCCATGCTA	ATCAAAACCTTCCACACCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019826	73628	74716	2116-2116	0.500000	59.8
clpP-psbB_p5	clpP-psbB	5	GAACTCGAAACGGCACTTTG	ATCAAAACCTTCCACACCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028399	73628	74716	1699-1699	0.500000	59.8
rps8-rpl14_p1	rps8-rpl14	1	TCAGCATTGCGTATCAAGGT	TGTTTCATTAGCACCCGAGG	20	20	45.0	50.0	57.6	57.9	2.4	0.0	0.509533	83209	83931	801-835	1.000000	79.2
rps8-rpl14_p2	rps8-rpl14	2	TCAGCATTGCGTATCAAGGT	GTTTCATTAGCACCCGAGGT	20	20	45.0	50.0	57.6	57.9	2.4	0.0	0.511008	83209	83931	800-834	1.000000	79.2
rps8-rpl14_p3	rps8-rpl14	3	GTCAGCATTGCGTATCAAGG	TGTTTCATTAGCACCCGAGG	20	20	50.0	50.0	57.3	57.9	2.4	0.0	0.852737	83209	83931	802-836	1.000000	76.5
rps8-rpl14_p4	rps8-rpl14	4	GTCAGCATTGCGTATCAAGG	GTTTCATTAGCACCCGAGGT	20	20	50.0	50.0	57.3	57.9	2.4	0.0	0.854212	83209	83931	801-835	1.000000	76.5
rps8-rpl14_p5	rps8-rpl14	5	TCAGCATTGCGTATCAAGGT	CCGGGAATTGAGACAGTTGA	20	20	45.0	50.0	57.6	57.5	2.4	0.0	0.874279	83209	83931	834-868	1.000000	76.2
ndhA_p1	ndhA	1	CACCGGGTTTTATCAATTTGCA	ACCCAGTTACCATAAGGAACA	22	21	40.9	42.9	58.1	56.3	6.3	0.0	4.829145	119145	121448	2521-2525	1.000000	42.2
ndhA_p2	ndhA	2	GTCCACCGGGTTTTATCAAT	ACCCAGTTACCATAAGGAACA	20	21	45.0	42.9	55.7	56.3	6.3	0.0	5.040669	119145	121448	2524-2528	1.000000	40.8
ndhA_p3	ndhA	3	ACCGGGTTTTATCAATTTGCA	ACCCAGTTACCATAAGGAACA	21	21	38.1	42.9	56.7	56.3	6.3	0.0	5.065345	119145	121448	2520-2524	1.000000	40.8
ndhA_p4	ndhA	4	CACCGGGTTTTATCAATTTGCA	AACCCAGTTACCATAAGGAACA	22	22	40.9	40.9	58.1	57.0	6.3	0.0	5.086037	119145	121448	2522-2526	1.000000	40.8
ndhA_p5	ndhA	5	CACCGGGTTTTATCAATTTGCA	TGAACCCAGTTACCATAAGGA	22	21	40.9	42.9	58.1	56.0	6.3	0.0	5.113193	119145	121448	2524-2528	1.000000	40.8
psaC-ndhD_p1	psaC-ndhD	1	CAGAGGACTGTGTTGGTTGT	ATTAAGGAACCCCCGGCTAT	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.502925	124123	124242	298-298	1.000000	77.3
psaC-ndhD_p2	psaC-ndhD	2	CAGAGGACTGTGTTGGTTGT	CCCGGCTATTGGCAAAACTA	20	20	50.0	50.0	57.7	58.2	0.0	3.0	0.574381	124123	124242	287-287	1.000000	76.8
psaC-ndhD_p3	psaC-ndhD	3	CAGAGGACTGTGTTGGTTGT	AATTAAGGAACCCCCGGCTA	20	20	50.0	50.0	57.7	58.4	0.0	0.0	0.733194	124123	124242	299-299	1.000000	75.5
psaC-ndhD_p4	psaC-ndhD	4	CAGAGGACTGTGTTGGTTGT	TTAAGGAACCCCCGGCTATT	20	20	50.0	50.0	57.7	58.4	0.0	0.0	0.733194	124123	124242	297-297	1.000000	75.5
psaC-ndhD_p5	psaC-ndhD	5	CAGAGGACTGTGTTGGTTGT	AAAATTAAGGAACCCCCGGC	20	20	50.0	50.0	57.7	58.4	0.0	0.0	0.768139	124123	124242	301-301	1.000000	75.2
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AAGTAGCAAAGCCTTGGGTC	TGGACAGCATTGAAAGCCTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.089294	127174	127843	1302-1302	0.500000	59.3
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	AAGTAGCAAAGCCTTGGGTC	TTGGACAGCATTGAAAGCCT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.089294	127174	127843	1303-1303	0.500000	59.3
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	GAAGTAGCAAAGCCTTGGGT	TGGACAGCATTGAAAGCCTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.089294	127174	127843	1303-1303	0.500000	59.3
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	GAAGTAGCAAAGCCTTGGGT	TTGGACAGCATTGAAAGCCT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.089294	127174	127843	1304-1304	0.500000	59.3
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	ATGGGATTGTAGAGCGGGTA	TGGACAGCATTGAAAGCCTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.097462	127174	127843	1361-1361	0.500000	59.2