primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TGGCCAAGCAGCTAAAAAGA	TCCCTACCCTATTGACTGCA	20	20	45.0	50.0	58.0	57.4	0.6	0.0	0.567253	301	900	725-725	1.000000	75.5
psbA_p2	psbA	2	TGGCCAAGCAGCTAAAAAGA	ACCCTATTGACTGCAACCTC	20	20	45.0	50.0	58.0	57.2	0.6	0.0	0.795453	301	900	720-720	1.000000	73.6
psbA_p3	psbA	3	ACTGGCCAAGCAGCTAAAAA	TCCCTACCCTATTGACTGCA	20	20	45.0	50.0	58.3	57.4	7.0	0.0	0.857798	301	900	727-727	1.000000	73.1
psbA_p4	psbA	4	GCCATAGTGCTAATGCCCAA	TCCCTACCCTATTGACTGCA	20	20	50.0	50.0	58.3	57.4	0.0	0.0	0.870013	301	900	769-769	1.000000	73.0
psbA_p5	psbA	5	CTATTACTGGCCAAGCAGCT	TCCCTACCCTATTGACTGCA	20	20	50.0	50.0	57.7	57.4	7.0	0.0	0.899786	301	900	732-732	1.000000	72.8
matK_p1	matK	1	TGTAGGGATTGATCCTGGCA	ATAATCCAAGAGGGGTCCGG	20	20	50.0	55.0	58.1	58.6	9.7	0.0	0.672811	1722	3242	1598-1598	1.000000	74.6
matK_p2	matK	2	TTTCATTGCACACGGCTTTC	ATAATCCAAGAGGGGTCCGG	20	20	45.0	55.0	57.9	58.6	0.0	0.0	0.697484	1722	3242	1672-1672	1.000000	74.4
matK_p3	matK	3	GCACACGGCTTTCTCCATAT	ATAATCCAAGAGGGGTCCGG	20	20	50.0	55.0	58.3	58.6	0.0	0.0	0.896324	1722	3242	1665-1665	1.000000	72.8
matK_p4	matK	4	CTGTAGGGATTGATCCTGGC	ATAATCCAAGAGGGGTCCGG	20	20	55.0	55.0	57.4	58.6	9.7	0.0	1.122663	1722	3242	1599-1599	1.000000	71.0
matK_p5	matK	5	TTTTCATTGCACACGGCTTT	ATAATCCAAGAGGGGTCCGG	20	20	40.0	55.0	57.4	58.6	0.0	0.0	1.158090	1722	3242	1673-1673	1.000000	70.7
matK-trnK-UUU_p1	matK-trnK-UUU	1	TGCGTAAAGATCTTCCCGAA	TCAATGGTAGAGTACTCGGCT	20	21	45.0	47.6	57.0	58.0	23.3	17.1	2.056877	3243	3884	752-752	1.000000	63.5
matK-trnK-UUU_p2	matK-trnK-UUU	2	TTGCGTAAAGATCTTCCCGA	TCAATGGTAGAGTACTCGGCT	20	21	45.0	47.6	57.0	58.0	23.3	17.1	2.056877	3243	3884	753-753	1.000000	63.5
matK-trnK-UUU_p3	matK-trnK-UUU	3	TGCGTAAAGATCTTCCCGAA	AGGGTTGCTAACTCAATGGT	20	20	45.0	45.0	57.0	56.7	23.3	2.5	2.318220	3243	3884	764-764	1.000000	61.5
matK-trnK-UUU_p4	matK-trnK-UUU	4	TTGCGTAAAGATCTTCCCGA	AGGGTTGCTAACTCAATGGT	20	20	45.0	45.0	57.0	56.7	23.3	2.5	2.318220	3243	3884	765-765	1.000000	61.5
matK-trnK-UUU_p5	matK-trnK-UUU	5	TGCGTAAAGATCTTCCCGAAA	TCAATGGTAGAGTACTCGGCT	21	21	42.9	47.6	57.7	58.0	23.3	17.1	2.336515	3243	3884	752-752	1.000000	61.3
trnK-UUU-chlB_p1	trnK-UUU-chlB	1	AGTCGCACTTAAAAGCCGAG	TTGCACGACAAAAGGGGATT	20	20	50.0	45.0	58.6	58.3	6.4	0.0	0.872031	3922	5313	1511-1511	1.000000	73.0
trnK-UUU-chlB_p2	trnK-UUU-chlB	2	AGTCGCACTTAAAAGCCGAG	TTTGCACGACAAAAGGGGAT	20	20	50.0	45.0	58.6	58.3	6.4	6.0	0.872031	3922	5313	1512-1512	1.000000	73.0
trnK-UUU-chlB_p3	trnK-UUU-chlB	3	TAGTCGCACTTAAAAGCCGA	TTGCACGACAAAAGGGGATT	20	20	45.0	45.0	57.3	58.3	3.0	0.0	1.030184	3922	5313	1512-1512	1.000000	71.8
trnK-UUU-chlB_p4	trnK-UUU-chlB	4	TCGCACTTAAAAGCCGAGTA	TTGCACGACAAAAGGGGATT	20	20	45.0	45.0	57.3	58.3	3.0	0.0	1.030184	3922	5313	1509-1509	1.000000	71.8
trnK-UUU-chlB_p5	trnK-UUU-chlB	5	TCGCACTTAAAAGCCGAGTA	TTTGCACGACAAAAGGGGAT	20	20	45.0	45.0	57.3	58.3	3.0	6.0	1.030184	3922	5313	1510-1510	1.000000	71.8
rps4_p1	rps4	1	TCCTCTTTCCAACATTATTTGTTCT	ACAGAGGAACAGATGTGCAT	25	20	32.0	45.0	56.9	56.8	0.0	0.0	7.243766	7549	8154	698-698	1.000000	40.0
rps4_p2	rps4	2	TCCTCTTTCCAACATTATTTGTTC	ACAGAGGAACAGATGTGCAT	24	20	33.3	45.0	55.6	56.8	0.0	0.0	7.561412	7549	8154	698-698	1.000000	40.0
rps4_p3	rps4	3	TCCTCTTTCCAACATTATTTGT	ACAGAGGAACAGATGTGCAT	22	20	31.8	45.0	53.6	56.8	0.0	0.0	7.561541	7549	8154	698-698	1.000000	40.0
rps4_p4	rps4	4	CCTCTTTCCAACATTATTTGTTCT	ACAGAGGAACAGATGTGCAT	24	20	33.3	45.0	55.6	56.8	0.0	0.0	7.571400	7549	8154	697-697	1.000000	40.0
rps4_p5	rps4	5	TCCTCTTTCCAACATTATTTGTTCTT	ACAGAGGAACAGATGTGCAT	26	20	30.8	45.0	57.5	56.8	0.0	0.0	7.644838	7549	8154	698-698	1.000000	40.0
psbC_p1	psbC	1	CCGAGCACCAAAGATTCAGT	GAGATTCGTGCAGCAGAAGA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.188299	17420	18841	1595-1595	1.000000	78.5
psbC_p2	psbC	2	CCGAGCACCAAAGATTCAGT	GTGCAGCAGAAGATCCTGAA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.291493	17420	18841	1588-1588	1.000000	77.7
psbC_p3	psbC	3	CCGAGCACCAAAGATTCAGT	AGGTATTCGTGCTTGGATGG	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.518550	17420	18841	1530-1530	1.000000	75.9
psbC_p4	psbC	4	CCGAGCACCAAAGATTCAGT	ATTCGTGCAGCAGAAGATCC	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.525146	17420	18841	1592-1592	1.000000	75.8
psbC_p5	psbC	5	CCGAGCACCAAAGATTCAGT	TGTTTCCCAGGAGATTCGTG	20	20	50.0	50.0	58.2	57.5	0.0	0.0	0.659182	17420	18841	1605-1605	1.000000	74.7
psbD_p1	psbD	1	GCCACATGAGCTCCAAGTAA	TGAAAGGGATGATGGATGAA	20	20	50.0	40.0	57.9	54.0	12.1	0.0	4.144686	18789	19850	1285-1285	1.000000	46.8
psbD_p2	psbD	2	GCCACATGAGCTCCAAGTAA	TTGAAAGGGATGATGGATGA	20	20	50.0	40.0	57.9	54.0	12.1	0.0	4.144686	18789	19850	1286-1286	1.000000	46.8
psbD_p3	psbD	3	CACCAAGCGAAACCAGTAGT	TGAAAGGGATGATGGATGAA	20	20	50.0	40.0	58.1	54.0	0.0	0.0	4.151873	18789	19850	1228-1228	1.000000	46.8
psbD_p4	psbD	4	CACCAAGCGAAACCAGTAGT	TTGAAAGGGATGATGGATGA	20	20	50.0	40.0	58.1	54.0	0.0	0.0	4.151873	18789	19850	1229-1229	1.000000	46.8
psbD_p5	psbD	5	GCCACATGAGCTCCAAGTAA	TGAAAGGGATGATGGATGAAA	20	21	50.0	38.1	57.9	54.9	12.1	0.0	4.257751	18789	19850	1285-1285	1.000000	45.9
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	AATAAACCGGACCAACCCAC	GTAACGCCATGGTAAGACGT	20	20	50.0	50.0	58.1	58.0	9.5	14.7	0.086651	19851	20818	1128-1128	1.000000	79.3
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	CAATAAACCGGACCAACCCA	GTAACGCCATGGTAAGACGT	20	20	50.0	50.0	58.1	58.0	9.5	14.7	0.088012	19851	20818	1129-1129	1.000000	79.3
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	ACAAAACGGTCTCTGCGTAG	GTAACGCCATGGTAAGACGT	20	20	50.0	50.0	58.2	58.0	0.0	14.7	0.220860	19851	20818	1104-1104	1.000000	78.2
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	AACAAAACGGTCTCTGCGTA	GTAACGCCATGGTAAGACGT	20	20	45.0	50.0	57.8	58.0	0.0	14.7	0.228567	19851	20818	1105-1105	1.000000	78.2
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	CGTAGCCAGTCATCCACAAT	GTAACGCCATGGTAAGACGT	20	20	50.0	50.0	57.7	58.0	0.0	14.7	0.316354	19851	20818	1089-1089	1.000000	77.5
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	TTAGAGTACCGCCCTGTCAA	TGAGGCTTCGTTTGTGCATA	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.249906	21506	22185	830-830	1.000000	78.0
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	ATTGGTTAGAGTACCGCCCT	TGAGGCTTCGTTTGTGCATA	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.301906	21506	22185	835-835	1.000000	77.6
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	TTAGAGTACCGCCCTGTCAA	CTTTGAGGCTTCGTTTGTGC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.313729	21506	22185	833-833	1.000000	77.5
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	TGTTTTACACGACGCAGCTA	TGAGGCTTCGTTTGTGCATA	20	20	45.0	45.0	57.9	57.8	0.0	0.0	0.314523	21506	22185	895-895	1.000000	77.5
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	ATTGGTTAGAGTACCGCCCT	CTTTGAGGCTTCGTTTGTGC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.365729	21506	22185	838-838	1.000000	77.1
accD-ycf1_p1	accD-ycf1	1	CATGGTTCCACGAGCTCTTT	ATATATTTGCGCCGGGCC	20	18	50.0	55.6	58.2	58.0	12.8	14.5	2.189873	103159	104249	1226-1287	1.000000	66.4
accD-ycf1_p2	accD-ycf1	2	CATGGTTCCACGAGCTCTTT	TCCTTCTATCCAGCGAATGA	20	20	50.0	45.0	58.2	55.7	12.8	0.0	2.448028	103159	104249	1151-1212	1.000000	64.4
accD-ycf1_p3	accD-ycf1	3	ATGGTTCCACGAGCTCTTTT	ATATATTTGCGCCGGGCC	20	18	45.0	55.6	57.4	58.0	12.8	14.5	2.572009	103159	104249	1225-1286	1.000000	63.3
accD-ycf1_p4	accD-ycf1	4	TGATAGCATGGTTCCACGAG	ATATATTTGCGCCGGGCC	20	18	50.0	55.6	57.4	58.0	1.1	14.5	2.605643	103159	104249	1232-1293	1.000000	63.0
accD-ycf1_p5	accD-ycf1	5	CATGGTTCCACGAGCTCTTT	GATCCTTCTATCCAGCGAATGA	20	22	50.0	45.5	58.2	57.5	12.8	0.0	2.713996	103159	104249	1153-1214	1.000000	62.3