primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TTGCTCCATTCCTTCAACGA	GTGTGCTTGGGAGTTCTTGA	20	20	45.0	50.0	57.4	58.0	0.0	0.0	0.592379	137	1198	1151-1151	1.000000	75.3
psbA_p2	psbA	2	TTGCTCCATTCCTTCAACGA	ATACGTGTGCTTGGGAGTTC	20	20	45.0	50.0	57.4	57.9	0.0	0.0	0.654460	137	1198	1155-1155	1.000000	74.8
psbA_p3	psbA	3	TGCTCCATTCCTTCAACGAT	GTGTGCTTGGGAGTTCTTGA	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.820154	137	1198	1150-1150	1.000000	73.4
psbA_p4	psbA	4	TGCTCCATTCCTTCAACGAT	ATACGTGTGCTTGGGAGTTC	20	20	45.0	50.0	57.2	57.9	0.0	0.0	0.882235	137	1198	1154-1154	1.000000	72.9
psbA_p5	psbA	5	TGCTCCATTCCTTCAACGATT	GTGTGCTTGGGAGTTCTTGA	21	20	42.9	50.0	57.9	58.0	0.0	0.0	1.104555	137	1198	1150-1150	1.000000	71.2
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACTCCTTAAAGAGAAGCGGA	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	55.9	58.2	8.1	17.1	2.280619	3269	4071	906-906	1.000000	61.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACTCCTTAAAGAGAAGCGGA	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	55.9	58.2	8.1	17.1	2.280619	3269	4071	905-905	1.000000	61.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACTCCTTAAAGAGAAGCGGA	GTCGTGCAGTGCAATTCAAT	20	20	45.0	45.0	55.9	57.7	8.1	21.7	2.448754	3269	4071	996-996	1.000000	60.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACTCCTTAAAGAGAAGCGGA	GGGTTGCTAACTCAACGGTA	20	20	45.0	50.0	55.9	57.5	8.1	0.0	2.572956	3269	4071	919-919	1.000000	59.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTCCTTAAAGAGAAGCGGA	TTCGGGTTGCTAACTCAACG	20	20	45.0	50.0	55.9	58.5	8.1	0.0	2.607840	3269	4071	922-922	1.000000	59.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCTGGACGAATCTGTTGC	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	57.9	57.9	0.0	0.0	1.225083	4109	4848	911-911	1.000000	70.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GTCTGGACGAATCTGTTGCT	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	57.9	57.9	0.0	0.0	1.225083	4109	4848	910-910	1.000000	70.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AGTCTGGACGAATCTGTTGC	GTGCTCAACCTACGGGAAC	20	19	50.0	57.9	57.9	58.2	0.0	0.0	1.249110	4109	4848	913-913	1.000000	70.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GTCTGGACGAATCTGTTGCT	GTGCTCAACCTACGGGAAC	20	19	50.0	57.9	57.9	58.2	0.0	0.0	1.249110	4109	4848	912-912	1.000000	70.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	58.2	57.9	20.3	0.0	1.307449	4109	4848	854-854	1.000000	69.5
rps16_p1	rps16	1	GCAAGAAAAAGGCAAATTGTGT	CCGGTTGAGCCAATGACTAT	22	20	36.4	50.0	57.1	57.7	0.0	0.0	3.212950	4849	5993	1320-1320	1.000000	54.3
rps16_p2	rps16	2	GCAAGAAAAAGGCAAATTGTGT	TATACACACACCGGTTGAGC	22	20	36.4	50.0	57.1	57.6	0.0	19.4	3.254427	4849	5993	1330-1330	1.000000	54.0
rps16_p3	rps16	3	ACAGCAAGAAAAAGGCAAATTG	CCGGTTGAGCCAATGACTAT	22	20	36.4	50.0	56.9	57.7	0.0	0.0	3.480732	4849	5993	1323-1323	1.000000	52.2
rps16_p4	rps16	4	AGCAAGAAAAAGGCAAATTGTG	CCGGTTGAGCCAATGACTAT	22	20	36.4	50.0	56.9	57.7	0.0	0.0	3.480732	4849	5993	1321-1321	1.000000	52.2
rps16_p5	rps16	5	CAGCAAGAAAAAGGCAAATTGT	CCGGTTGAGCCAATGACTAT	22	20	36.4	50.0	56.9	57.7	0.0	0.0	3.480732	4849	5993	1322-1322	1.000000	52.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.104521	5994	7107	1205-1205	1.000000	79.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.105939	5994	7107	1203-1203	1.000000	79.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	5994	7107	1211-1211	1.000000	79.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GAATCCACAGCCGATCATGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	5994	7107	1212-1212	1.000000	79.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAGCCGATCATGTCCTTCAA	GAAATTCGAAATGGGGCGTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.120355	5994	7107	1264-1264	1.000000	79.0
trnG-UCC_p1	trnG-UCC	1	TGATTGCGCCTCACTCTTTT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.1	57.2	4.2	18.0	3.896810	8968	9725	877-877	1.000000	48.8
trnG-UCC_p2	trnG-UCC	2	TTGATTGCGCCTCACTCTTT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.1	57.2	4.2	18.0	3.896810	8968	9725	878-878	1.000000	48.8
trnG-UCC_p3	trnG-UCC	3	GATTGCGCCTCACTCTTTTG	TGCATATCCAAATTGATGATGCA	20	23	50.0	34.8	57.7	57.2	4.2	18.0	4.046422	8968	9725	876-876	1.000000	47.6
trnG-UCC_p4	trnG-UCC	4	ATCTTGATTGCGCCTCACTC	TGCATATCCAAATTGATGATGCA	20	23	50.0	34.8	58.3	57.2	4.2	18.0	4.131689	8968	9725	881-881	1.000000	46.9
trnG-UCC_p5	trnG-UCC	5	ATTGCGCCTCACTCTTTTGT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.4	57.2	4.2	18.0	4.183822	8968	9725	875-875	1.000000	46.5
rpl16_p1	rpl16	1	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACCA	20	26	50.0	30.8	57.9	58.1	0.0	0.0	6.217863	82800	84345	1639-1650	1.000000	40.6
rpl16_p2	rpl16	2	GTCCAGAATAAACCTGCGGT	GGTTGAATAAAAATTCGATTGACCA	20	25	50.0	32.0	57.9	56.9	0.0	0.0	6.223742	82800	84345	1638-1649	1.000000	40.6
rpl16_p3	rpl16	3	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACC	20	25	50.0	32.0	57.9	56.6	0.0	0.0	6.464149	82800	84345	1639-1650	1.000000	40.6
rpl16_p4	rpl16	4	TCCGTCCAGAATAAACCTGC	AGGTTGAATAAAAATTCGATTGACCA	20	26	50.0	30.8	57.6	58.1	0.0	0.0	6.505028	82800	84345	1642-1653	1.000000	40.6
rpl16_p5	rpl16	5	TCCGTCCAGAATAAACCTGC	GGTTGAATAAAAATTCGATTGACCA	20	25	50.0	32.0	57.6	56.9	0.0	0.0	6.510907	82800	84345	1641-1652	1.000000	40.6
rpl22-rps19_p1	rpl22-rps19	1	CGTCCGCGAATCTGATCAAT	TCTGACTTTCGCGAGACATG	20	20	50.0	50.0	58.5	58.0	8.2	9.6	0.525718	85621	85760	305-305	1.000000	75.8
rpl22-rps19_p2	rpl22-rps19	2	CGTCCGCGAATCTGATCAAT	CCTACTCTGACTTTCGCGAG	20	20	50.0	55.0	58.5	57.8	8.2	9.6	0.689756	85621	85760	310-310	1.000000	74.5
rpl22-rps19_p3	rpl22-rps19	3	CGTCCGCGAATCTGATCAAT	CTCTGACTTTCGCGAGACAT	20	20	50.0	50.0	58.5	57.7	8.2	9.6	0.778605	85621	85760	306-306	1.000000	73.8
rpl22-rps19_p4	rpl22-rps19	4	CGTCCGCGAATCTGATCAAT	CAAATTGGGAGAATTCGCGC	20	20	50.0	50.0	58.5	58.5	8.2	17.5	0.959344	85621	85760	329-329	1.000000	72.3
rpl22-rps19_p5	rpl22-rps19	5	CGTCCGCGAATCTGATCAAT	TTCGCGAGACATGCAAGAAA	20	20	50.0	45.0	58.5	58.5	8.2	9.6	1.000768	85621	85760	298-298	1.000000	72.0
rps19_p1	rps19	1	GCGAACCAAAAGGAACGATG	GGGTAGAGCCGGATCTAAGT	20	20	50.0	55.0	58.0	58.0	0.0	29.9	0.038031	85761	86039	1408-1408	1.000000	79.9
rps19_p2	rps19	2	GCGAACCAAAAGGAACGATG	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	58.0	58.0	0.0	29.9	0.039455	85761	86039	1409-1409	1.000000	79.9
rps19_p3	rps19	3	GCGAACCAAAAGGAACGATG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	58.0	58.0	0.0	22.4	0.060429	85761	86039	1271-1271	1.000000	79.7
rps19_p4	rps19	4	TGCTGCTGCAGAATAAACCA	GGGTAGAGCCGGATCTAAGT	20	20	45.0	55.0	58.1	58.0	32.3	29.9	0.093434	85761	86039	1093-1093	1.000000	79.5
rps19_p5	rps19	5	GCGAACCAAAAGGAACGATG	GAGGGTAAATCGGCCACATT	20	20	50.0	50.0	58.0	57.9	0.0	2.2	0.089035	85761	86039	1521-1521	1.000000	79.4
rps19-trnH-GUG_p1	rps19-trnH-GUG	1	TGCCCGAGACCAAGTTACTA	GATCCACTTGGCTACATCCG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.201432	86040	86175	268-268	1.000000	79.3
rps19-trnH-GUG_p2	rps19-trnH-GUG	2	TGCCCGAGACCAAGTTACTA	CAATCCACTGCCTTGATCCA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.205794	86040	86175	282-282	1.000000	79.2
rps19-trnH-GUG_p3	rps19-trnH-GUG	3	TGCCCGAGACCAAGTTACTA	AATCCACTGCCTTGATCCAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.207302	86040	86175	281-281	1.000000	79.2
rps19-trnH-GUG_p4	rps19-trnH-GUG	4	TGCCCGAGACCAAGTTACTA	ACAATCCACTGCCTTGATCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.207302	86040	86175	283-283	1.000000	79.2
rps19-trnH-GUG_p5	rps19-trnH-GUG	5	TGCCCGAGACCAAGTTACTA	TAATGTGATGGGCGAACGAC	20	20	50.0	50.0	58.1	58.4	0.0	0.0	0.425167	86040	86175	331-331	1.000000	77.3
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	20	20	45.0	50.0	57.7	57.9	16.5	0.0	0.380474	100834	102801	2101-2125	1.000000	77.9
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	100834	102801	2040-2064	1.000000	77.6
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	100834	102801	2044-2068	1.000000	77.5
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	100834	102801	2039-2063	1.000000	77.5
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	100834	102801	2042-2066	1.000000	76.6
rps15-ycf1_p1	rps15-ycf1	1	TCAACAGAACCCTTGTTTTCT	TGGCCCAATTACCGATTAGA	21	20	38.1	45.0	55.5	56.3	0.0	0.0	5.161573	125891	126278	576-576	1.000000	40.4
rps15-ycf1_p2	rps15-ycf1	2	TCAACAGAACCCTTGTTTTCTTC	TGGCCCAATTACCGATTAGA	23	20	39.1	45.0	57.4	56.3	0.0	0.0	5.280245	125891	126278	576-576	1.000000	40.4
rps15-ycf1_p3	rps15-ycf1	3	CAACAGAACCCTTGTTTTCTTCT	TGGCCCAATTACCGATTAGA	23	20	39.1	45.0	57.4	56.3	0.0	0.0	5.286487	125891	126278	575-575	1.000000	40.4
rps15-ycf1_p4	rps15-ycf1	4	TGAAATTCAACAGAACCCTTGT	TGGCCCAATTACCGATTAGA	22	20	36.4	45.0	56.4	56.3	0.0	0.0	5.330583	125891	126278	582-582	1.000000	40.4
rps15-ycf1_p5	rps15-ycf1	5	TCAACAGAACCCTTGTTTTCT	TGGCCCAATTACCGATTAGAA	21	21	38.1	42.9	55.5	57.1	0.0	0.0	5.393593	125891	126278	576-576	1.000000	40.4
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	CGGGGGAGCAAGTCAAAATA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.9	57.7	0.0	16.5	0.380474	140776	142743	2101-2125	1.000000	77.9
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	140776	142743	2040-2064	1.000000	77.6
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	140776	142743	2044-2068	1.000000	77.5
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	140776	142743	2039-2063	1.000000	77.5
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	140776	142743	2042-2066	1.000000	76.6
rpl2_p1	rpl2	1	CGCTTCAACCGGGTTATTCT	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.3	58.1	13.0	0.2	0.394757	155798	157281	1671-1671	1.000000	76.8
rpl2_p2	rpl2	2	CGCTTCAACCGGGTTATTCT	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.3	57.9	13.0	0.0	0.399119	155798	157281	1657-1657	1.000000	76.8
rpl2_p3	rpl2	3	CGCTTCAACCGGGTTATTCT	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.3	57.9	13.0	0.0	0.400626	155798	157281	1656-1656	1.000000	76.8
rpl2_p4	rpl2	4	CGCTTCAACCGGGTTATTCT	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.3	57.9	13.0	0.0	0.400626	155798	157281	1658-1658	1.000000	76.8
rpl2_p5	rpl2	5	ATCATTACGCTTCAACCGGG	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.3	58.1	6.3	0.2	0.465225	155798	157281	1678-1678	1.000000	76.3
trnH-GUG-rps19_p1	trnH-GUG-rps19	1	GATCCACTTGGCTACATCCG	TGCCCGAGACCAAGTTACTA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.201432	157402	157537	268-268	1.000000	79.3
trnH-GUG-rps19_p2	trnH-GUG-rps19	2	CAATCCACTGCCTTGATCCA	TGCCCGAGACCAAGTTACTA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.205794	157402	157537	282-282	1.000000	79.2
trnH-GUG-rps19_p3	trnH-GUG-rps19	3	AATCCACTGCCTTGATCCAC	TGCCCGAGACCAAGTTACTA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.207302	157402	157537	281-281	1.000000	79.2
trnH-GUG-rps19_p4	trnH-GUG-rps19	4	ACAATCCACTGCCTTGATCC	TGCCCGAGACCAAGTTACTA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.207302	157402	157537	283-283	1.000000	79.2
trnH-GUG-rps19_p5	trnH-GUG-rps19	5	TAATGTGATGGGCGAACGAC	TGCCCGAGACCAAGTTACTA	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.425167	157402	157537	331-331	1.000000	77.3