primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CACCGGGCATTCAAAAAGTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.5	58.0	6.3	0.0	0.532399	175	927	837-840	1.000000	77.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CACCGGGCATTCAAAAAGTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.5	58.0	6.3	0.0	0.533769	175	927	838-841	1.000000	77.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTCCACCGGGCATTCAAAAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.6	58.0	6.3	0.0	0.593672	175	927	840-843	1.000000	76.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CACCGGGCATTCAAAAAGTT	ATCCGCATAAAAGAGCTGCA	20	20	45.0	45.0	57.5	58.0	6.3	1.0	0.567255	175	927	1423-1426	1.000000	76.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CACCGGGCATTCAAAAAGTT	TCCGCATAAAAGAGCTGCAT	20	20	45.0	45.0	57.5	58.0	6.3	1.0	0.567255	175	927	1422-1425	1.000000	76.2
ycf1_p1	ycf1	1	TCTTGTCTTTTGTGATTCGTTCT	AGGAAATTCAAAAAGTAAACCCA	23	23	34.8	30.4	56.7	54.9	0.0	0.0	10.457639	13153	13272	237-237	1.000000	41.4
ycf1_p2	ycf1	2	TCTTGTCTTTTGTGATTCGTTCT	AGGAAATTCAAAAAGTAAACCCAA	23	24	34.8	29.2	56.7	55.6	0.0	0.0	10.721870	13153	13272	237-237	1.000000	41.4
ycf1_p3	ycf1	3	TCTTGTCTTTTGTGATTCGTTCT	TGAAGGAAATTCAAAAAGTAAACCCA	23	26	34.8	30.8	56.7	58.1	0.0	0.0	10.470409	13153	13272	240-240	1.000000	41.3
ycf1_p4	ycf1	4	TCTTGTCTTTTGTGATTCGTTCT	GAAGGAAATTCAAAAAGTAAACCCA	23	25	34.8	32.0	56.7	56.7	0.0	0.0	10.649480	13153	13272	239-239	1.000000	41.3
ycf1_p5	ycf1	5	TCTTGTCTTTTGTGATTCGTTCT	TGAAGGAAATTCAAAAAGTAAACCC	23	25	34.8	32.0	56.7	56.7	0.0	0.0	10.649480	13153	13272	240-240	1.000000	41.3
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCATGATCATGAGCAAACGC	TTTGATAGGGTCGTGCAGTG	20	20	50.0	50.0	57.9	57.9	32.7	0.0	0.208654	45022	45844	1037-1040	1.000000	78.6
matK-trnK-UUU_p2	matK-trnK-UUU	2	CCATGATCATGAGCAAACGC	ATCCGGGTTGCTAACTCAAC	20	20	50.0	50.0	57.9	57.9	32.7	0.0	0.224773	45022	45844	970-973	1.000000	78.5
matK-trnK-UUU_p3	matK-trnK-UUU	3	TAGGAAGTGTTGTTGCCGAG	TTTGATAGGGTCGTGCAGTG	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.257124	45022	45844	987-990	1.000000	78.3
matK-trnK-UUU_p4	matK-trnK-UUU	4	TAGGAAGTGTTGTTGCCGAG	ATCCGGGTTGCTAACTCAAC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273243	45022	45844	920-923	1.000000	78.2
matK-trnK-UUU_p5	matK-trnK-UUU	5	CCATGATCATGAGCAAACGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.9	58.2	32.7	17.1	0.283768	45022	45844	952-955	1.000000	78.1
psbK-psbI_p1	psbK-psbI	1	CAAGCCGCTGTAAGTTTTCG	GGATTGCGTCCTGGATCATT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.076656	48609	49028	539-542	1.000000	80.3
psbK-psbI_p2	psbK-psbI	2	AGCCGCTGTAAGTTTTCGAT	GGATTGCGTCCTGGATCATT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.121890	48609	49028	537-540	1.000000	79.9
psbK-psbI_p3	psbK-psbI	3	AAGCCGCTGTAAGTTTTCGA	GGATTGCGTCCTGGATCATT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.153430	48609	49028	538-541	1.000000	79.7
psbK-psbI_p4	psbK-psbI	4	GCCGCTGTAAGTTTTCGATG	GGATTGCGTCCTGGATCATT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.186595	48609	49028	536-539	1.000000	79.4
psbK-psbI_p5	psbK-psbI	5	GCAAGCCGCTGTAAGTTTTC	GGATTGCGTCCTGGATCATT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.340664	48609	49028	540-543	1.000000	78.2
atpF_p1	atpF	1	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTAATAGAA	20	20	45.0	50.0	57.4	57.5	9.6	0.0	1.146058	53216	54611	1569-1591	1.000000	71.0
atpF_p2	atpF	2	GGCTCGAAGGGTTACCATTA	GGGCGAGCGAAGTAATAGAA	20	20	50.0	50.0	57.0	57.5	0.0	0.0	1.498105	53216	54611	1557-1579	1.000000	68.2
atpF_p3	atpF	3	ACTAATTTCGTCGGCTCGAA	AAGGGGCGAGCGAAGTAATA	20	20	45.0	50.0	57.4	58.9	9.6	0.0	1.524988	53216	54611	1572-1594	1.000000	68.0
atpF_p4	atpF	4	GGCTCGAAGGGTTACCATTAG	GGGCGAGCGAAGTAATAGAA	21	20	52.4	50.0	58.2	57.5	0.0	0.0	1.668892	53216	54611	1557-1579	1.000000	66.9
atpF_p5	atpF	5	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTAATAGAAA	20	21	45.0	47.6	57.4	58.2	9.6	0.0	1.824238	53216	54611	1569-1591	1.000000	65.6
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	20	20	45.0	55.0	55.8	58.1	0.0	0.0	2.287973	72444	73474	1159-1161	1.000000	62.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	20	20	45.0	50.0	55.8	58.1	0.0	10.4	2.374003	72444	73474	1163-1165	1.000000	61.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCAGTAGC	CATACACCTCGCTGGGATTG	20	20	45.0	55.0	55.8	58.4	0.0	0.0	2.642877	72444	73474	1165-1167	1.000000	59.2
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAGAATGAACAGTGCA	CCTCGCTGGGATTGTAGTTC	22	20	40.9	55.0	57.4	58.1	0.0	0.0	2.682607	72444	73474	1166-1168	1.000000	58.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.8	58.5	0.0	5.4	2.706630	72444	73474	1142-1144	1.000000	58.7
accD-rbcL_p1	accD-rbcL	1	ATCACTTCCACTGGTATGGC	CTAGCAAATGGAGCCCTGAG	20	20	50.0	55.0	57.3	58.0	0.0	0.0	0.753086	76196	76887	906-910	1.000000	74.5
accD-rbcL_p2	accD-rbcL	2	ATCACTTCCACTGGTATGGC	TGAAGCTAGCAAATGGAGCC	20	20	50.0	50.0	57.3	58.2	0.0	18.8	0.962460	76196	76887	911-915	1.000000	72.8
accD-rbcL_p3	accD-rbcL	3	ATCACTTCCACTGGTATGGC	TCGAGTTCGAACCGGTAGAT	20	20	50.0	50.0	57.3	58.3	0.0	27.3	0.974878	76196	76887	849-853	1.000000	72.8
accD-rbcL_p4	accD-rbcL	4	ATCACTTCCACTGGTATGGC	CCGTGAAGCTAGCAAATGGA	20	20	50.0	50.0	57.3	58.3	0.0	18.8	0.979801	76196	76887	914-918	1.000000	72.7
accD-rbcL_p5	accD-rbcL	5	ATCACTTCCACTGGTATGGC	TCCGTGAAGCTAGCAAATGG	20	20	50.0	50.0	57.3	58.3	0.0	18.8	0.979801	76196	76887	915-919	1.000000	72.7
rbcL-atpB_p1	rbcL-atpB	1	GGTGACATAAGTCCCTCCCT	GGGGAAAAACGACATCCAGT	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.213422	78349	79125	914-916	1.000000	79.0
rbcL-atpB_p2	rbcL-atpB	2	GGTGACATAAGTCCCTCCCT	TGGGCCAATAATTTGAGCGA	20	20	55.0	45.0	58.1	57.9	0.0	2.1	0.255358	78349	79125	892-894	1.000000	78.7
rbcL-atpB_p3	rbcL-atpB	3	TGGTGACATAAGTCCCTCCC	GGGGAAAAACGACATCCAGT	20	20	55.0	50.0	58.4	58.1	0.0	0.0	0.514303	78349	79125	915-917	1.000000	76.6
rbcL-atpB_p4	rbcL-atpB	4	TGGTGACATAAGTCCCTCCC	TGGGCCAATAATTTGAGCGA	20	20	55.0	45.0	58.4	57.9	0.0	2.1	0.556239	78349	79125	893-895	1.000000	76.3
rbcL-atpB_p5	rbcL-atpB	5	GGTGACATAAGTCCCTCCCT	CTGGGCCAATAATTTGAGCG	20	20	55.0	50.0	58.1	57.5	0.0	8.0	0.616166	78349	79125	893-895	1.000000	75.8
trnV-UAC-ndhC_p1	trnV-UAC-ndhC	1	AGAAGGTCTACGGTTCGAGT	CTAACCGGGGCTAAAACTCC	20	20	50.0	55.0	57.8	58.0	0.0	6.3	0.231387	82095	82896	938-944	1.000000	78.9
trnV-UAC-ndhC_p2	trnV-UAC-ndhC	2	GAGCCCAATGCAACAAACAA	CTAACCGGGGCTAAAACTCC	20	20	45.0	55.0	57.8	58.0	0.0	6.3	0.271497	82095	82896	1025-1031	1.000000	78.5
trnV-UAC-ndhC_p3	trnV-UAC-ndhC	3	AGAAGGTCTACGGTTCGAGT	CGGGGCTAAAACTCCTGAAA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.391484	82095	82896	933-939	1.000000	77.6
trnV-UAC-ndhC_p4	trnV-UAC-ndhC	4	GAGCCCAATGCAACAAACAA	CGGGGCTAAAACTCCTGAAA	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.431594	82095	82896	1020-1026	1.000000	77.3
trnV-UAC-ndhC_p5	trnV-UAC-ndhC	5	AGAAGGTCTACGGTTCGAGT	TAACCGGGGCTAAAACTCCT	20	20	50.0	50.0	57.8	58.3	0.0	6.3	0.537041	82095	82896	937-943	1.000000	76.5
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	ACCAATTTCGCCATATCCCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137364	86370	87140	795-822	1.000000	81.9
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	ACCAATTTCGCCATATCCCC	GAGTCGAACCGATGACCATC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.163174	86370	87140	826-853	1.000000	81.6
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	86370	87140	803-830	1.000000	81.4
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GAGTCGAACCGATGACCATC	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.227983	86370	87140	834-861	1.000000	81.1
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	ATCCAATCAAGTCCGTAGCG	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.357808	86370	87140	818-845	1.000000	80.1
ycf3-psaA_p1	ycf3-psaA	1	GTTGAAAATCACGAAGCGCT	CCCATTCCTCGAAAGACGTT	20	20	45.0	50.0	57.7	57.9	2.6	0.0	0.410881	91111	91743	735-747	1.000000	77.9
ycf3-psaA_p2	ycf3-psaA	2	GCTATAGCGCTTACTCCAGG	CCCATTCCTCGAAAGACGTT	20	20	55.0	50.0	57.6	57.9	18.7	0.0	0.450479	91111	91743	779-791	1.000000	77.5
ycf3-psaA_p3	ycf3-psaA	3	CCGATCAAGCTGCTGAGTAT	CCCATTCCTCGAAAGACGTT	20	20	50.0	50.0	57.5	57.9	1.4	0.0	0.617598	91111	91743	808-820	1.000000	76.1
ycf3-psaA_p4	ycf3-psaA	4	TCCGATCAAGCTGCTGAGTA	CCCATTCCTCGAAAGACGTT	20	20	50.0	50.0	58.5	57.9	1.4	0.0	0.620433	91111	91743	809-821	1.000000	76.1
ycf3-psaA_p5	ycf3-psaA	5	TGAAAATCACGAAGCGCTTC	CCCATTCCTCGAAAGACGTT	20	20	45.0	50.0	57.4	57.9	35.0	0.0	0.689291	91111	91743	733-745	1.000000	75.7
trnG-GCC-psbZ_p1	trnG-GCC-psbZ	1	AATCGAACCCGCATCTTCTC	TGCTTCTTCTGATGGTTGGC	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.520628	97109	97425	484-484	1.000000	76.5
trnG-GCC-psbZ_p2	trnG-GCC-psbZ	2	GAATCGAACCCGCATCTTCT	TGCTTCTTCTGATGGTTGGC	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.520628	97109	97425	485-485	1.000000	76.5
trnG-GCC-psbZ_p3	trnG-GCC-psbZ	3	AATCGAACCCGCATCTTCTC	TGGTTGGCCAAGTAACAAGA	20	20	50.0	45.0	58.1	57.3	0.0	21.2	0.797262	97109	97425	472-472	1.000000	74.3
trnG-GCC-psbZ_p4	trnG-GCC-psbZ	4	GAATCGAACCCGCATCTTCT	TGGTTGGCCAAGTAACAAGA	20	20	50.0	45.0	58.1	57.3	0.0	21.2	0.797262	97109	97425	473-473	1.000000	74.3
trnG-GCC-psbZ_p5	trnG-GCC-psbZ	5	AATCGAACCCGCATCTTCTC	TGGTTGGCCAAGTAACAAGAA	20	21	50.0	42.9	58.1	58.0	0.0	21.2	1.078474	97109	97425	472-472	1.000000	72.1
psbC_p1	psbC	1	CCGAGCCAGAACAGAAAAGA	TTTCCCAAGAAATCCGTGCA	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.181539	98174	99595	1582-1582	1.000000	78.7
psbC_p2	psbC	2	CCGAGCCAGAACAGAAAAGA	GCAGCGGAAGATCCTGAATT	20	20	50.0	50.0	57.8	58.3	0.0	0.6	0.496664	98174	99595	1565-1565	1.000000	76.2
psbC_p3	psbC	3	CCGAGCCAGAACAGAAAAGA	AGGTATTCGTGCTTGGATGG	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.501895	98174	99595	1509-1509	1.000000	76.1
psbC_p4	psbC	4	CCGAGCCAGAACAGAAAAGA	GTTTCCCAAGAAATCCGTGC	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.517530	98174	99595	1583-1583	1.000000	76.0
psbC_p5	psbC	5	CCGAGCCAGAACAGAAAAGA	TTCCCAAGAAATCCGTGCAG	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.636207	98174	99595	1581-1581	1.000000	75.1
psbD-psaI_p1	psbD-psaI	1	GGACCAGCCTACAAAAACGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.140117	100605	102276	1839-1874	1.000000	79.7
psbD-psaI_p2	psbD-psaI	2	GGACCAGCCTACAAAAACGA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.1	57.9	0.0	26.8	0.216651	100605	102276	1845-1880	1.000000	79.1
psbD-psaI_p3	psbD-psaI	3	ACAAAAACGAAACGGTCCCT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.2	58.0	0.0	26.9	0.273073	100605	102276	1829-1864	1.000000	78.7
psbD-psaI_p4	psbD-psaI	4	ACAAAAACGAAACGGTCCCT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	58.2	57.9	0.0	26.8	0.349607	100605	102276	1835-1870	1.000000	78.1
psbD-psaI_p5	psbD-psaI	5	CAAAAACGAAACGGTCCCTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.6	58.0	0.0	26.9	0.421549	100605	102276	1828-1863	1.000000	77.5
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.096671	106020	107152	1254-1260	1.000000	80.8
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	TGGGTTGGGTTCATCTCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.096671	106020	107152	1252-1258	1.000000	80.8
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTCGC	CTGGGTTGGGTTCATCTCTC	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.461827	106020	107152	1253-1259	1.000000	77.8
petA-psbJ_p4	petA-psbJ	4	CGTCCAAGGCCTTTTGTTCT	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	58.7	58.0	17.6	0.0	0.726216	106020	107152	1261-1267	1.000000	75.7
petA-psbJ_p5	petA-psbJ	5	CGTCCAAGGCCTTTTGTTCT	TGGGTTGGGTTCATCTCTCT	20	20	50.0	50.0	58.7	58.0	17.6	0.0	0.726216	106020	107152	1259-1265	1.000000	75.7
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	TAGACCCGCTAAAGACCCAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.158377	110223	110559	497-497	1.000000	78.9
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	TAGACCCGCTAAAGACCCAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.159721	110223	110559	496-496	1.000000	78.9
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	TAGACCCGCTAAAGACCCAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.166413	110223	110559	508-508	1.000000	78.8
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	TAGACCCGCTAAAGACCCAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.167747	110223	110559	507-507	1.000000	78.8
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	TAGACCCGCTAAAGACCCAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.167747	110223	110559	510-510	1.000000	78.8
rpl16-rps3_p1	rpl16-rps3	1	AGTTTATAGCGGGGGTCAGT	TCCCCTACAAACAATTCGCG	20	20	50.0	50.0	58.1	58.6	0.0	0.0	0.679676	126482	126626	335-338	1.000000	76.0
rpl16-rps3_p2	rpl16-rps3	2	AGCGGGGGTCAGTTTATTTT	TCCCCTACAAACAATTCGCG	20	20	45.0	50.0	57.4	58.6	0.0	0.0	1.152742	126482	126626	328-331	1.000000	72.2
rpl16-rps3_p3	rpl16-rps3	3	GTTTATAGCGGGGGTCAGTT	TCCCCTACAAACAATTCGCG	20	20	50.0	50.0	57.3	58.6	0.0	0.0	1.259629	126482	126626	334-337	1.000000	71.4
rpl16-rps3_p4	rpl16-rps3	4	AGTTTATAGCGGGGGTCAGT	CCCTACAAACAATTCGCGCT	20	20	50.0	50.0	58.1	59.2	0.0	5.6	1.322018	126482	126626	333-336	1.000000	70.9
rpl16-rps3_p5	rpl16-rps3	5	AGTTTATAGCGGGGGTCAGT	GTTCCCCTACAAACAATTCGC	20	21	50.0	47.6	58.1	57.8	0.0	0.0	1.336315	126482	126626	337-340	1.000000	70.7