primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
APQ78_gt002-APQ78_gp070_p1	APQ78_gt002-APQ78_gp070	1	GGAGAACAAAAGCTCGTTGC	AAGGCAGGGGTTTATCATCG	20	20	50.0	50.0	58.0	57.7	9.4	0.0	0.386352	4188	4742	976-986	0.500000	60.8
APQ78_gt002-APQ78_gp070_p2	APQ78_gt002-APQ78_gp070	2	AAAGCCGAGTACTCTACCGT	AAGGCAGGGGTTTATCATCG	20	20	50.0	50.0	58.2	57.7	20.3	0.0	0.516831	4188	4742	664-680	0.500000	60.6
APQ78_gt002-APQ78_gp070_p3	APQ78_gt002-APQ78_gp070	3	AAGCCGAGTACTCTACCGTT	AAGGCAGGGGTTTATCATCG	20	20	50.0	50.0	58.2	57.7	20.3	0.0	0.516831	4188	4742	663-679	0.500000	60.6
APQ78_gt002-APQ78_gp070_p4	APQ78_gt002-APQ78_gp070	4	AGCCGAGTACTCTACCGTTT	AAGGCAGGGGTTTATCATCG	20	20	50.0	50.0	58.2	57.7	20.3	0.0	0.516831	4188	4742	662-678	0.500000	60.6
APQ78_gt002-APQ78_gp070_p5	APQ78_gt002-APQ78_gp070	5	CCGCACTTAAAAGCCGAGTA	AAGGCAGGGGTTTATCATCG	20	20	50.0	50.0	58.3	57.7	5.3	0.0	0.631217	4188	4742	673-689	0.500000	59.6
APQ78_gp070-APQ78_gt003_p1	APQ78_gp070-APQ78_gt003	1	GCTAAGAGCACTTCCAGTCC	CAAAGGTCTGATCCGGATGG	20	20	55.0	55.0	58.0	58.0	0.0	24.8	0.058096	6044	6816	1491-1514	0.500000	64.7
APQ78_gp070-APQ78_gt003_p2	APQ78_gp070-APQ78_gt003	2	GCTAAGAGCACTTCCAGTCC	TGGTCCTGTTATTCGGAGGT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.070154	6044	6816	903-932	0.500000	64.7
APQ78_gp070-APQ78_gt003_p3	APQ78_gp070-APQ78_gt003	3	GCTAAGAGCACTTCCAGTCC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.0	58.1	0.0	16.9	0.093239	6044	6816	888-917	0.500000	64.6
APQ78_gp070-APQ78_gt003_p4	APQ78_gp070-APQ78_gt003	4	CGAGCTAAGAGCACTTCCAG	CAAAGGTCTGATCCGGATGG	20	20	55.0	55.0	58.1	58.0	0.0	24.8	0.121040	6044	6816	1494-1517	0.500000	64.1
APQ78_gp070-APQ78_gt003_p5	APQ78_gp070-APQ78_gt003	5	TTTCTACTCGAGCTCCACCA	CAAAGGTCTGATCCGGATGG	20	20	50.0	55.0	58.1	58.0	13.6	24.8	0.121718	6044	6816	1616-1639	0.500000	64.1
APQ78_gt004-APQ78_gt005_p1	APQ78_gt004-APQ78_gt005	1	GGGATTTGAACCCTCGGTAC	GAGTTGGATCTGCAGCTCAA	20	20	55.0	50.0	58.0	57.9	0.0	18.6	0.139110	7992	8410	1214-2243	1.000000	90.9
APQ78_gt004-APQ78_gt005_p2	APQ78_gt004-APQ78_gt005	2	GGGATTTGAACCCTCGGTAC	CCATGAAACAAGTAGCCGGA	20	20	55.0	50.0	58.0	57.9	0.0	5.0	0.142054	7992	8410	1187-2216	1.000000	90.9
APQ78_gt004-APQ78_gt005_p3	APQ78_gt004-APQ78_gt005	3	GGGATTTGAACCCTCGGTAC	AACTCAGGAAGATGAACGCC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.134709	7992	8410	868-1897	0.833333	84.3
APQ78_gt004-APQ78_gt005_p4	APQ78_gt004-APQ78_gt005	4	CCATCCGGATCAGACCTTTG	AACTCAGGAAGATGAACGCC	20	20	55.0	50.0	58.0	57.9	15.9	0.0	0.141563	7992	8410	1387-1705	0.500000	66.4
APQ78_gt004-APQ78_gt005_p5	APQ78_gt004-APQ78_gt005	5	CCATCCGGATCAGACCTTTG	GAGTTGGATCTGCAGCTCAA	20	20	55.0	50.0	58.0	57.9	15.9	18.6	0.145965	7992	8410	1733-2051	0.500000	65.1
APQ78_gp066_p1	APQ78_gp066	1	ACCTAAAAACGCCTTGCTCA	AGGCCAAGCTGTAGAAGGTA	20	20	45.0	50.0	58.0	58.0	0.0	5.0	0.071719	10303	11656	2269-2308	0.833333	84.8
APQ78_gp066_p2	APQ78_gp066	2	AACACGACCTAAAAACGCCT	AGGCCAAGCTGTAGAAGGTA	20	20	45.0	50.0	58.0	58.0	0.0	5.0	0.089710	10303	11656	2275-2314	0.833333	84.6
APQ78_gp066_p3	APQ78_gp066	3	CACCCGCCATTACTTCATCA	AGGCCAAGCTGTAGAAGGTA	20	20	50.0	50.0	58.0	58.0	0.0	5.0	0.084336	10303	11656	1823-2144	0.666667	75.5
APQ78_gp066_p4	APQ78_gp066	4	ACACGACCTAAAAACGCCTT	AGGCCAAGCTGTAGAAGGTA	20	20	45.0	50.0	58.0	58.0	0.0	5.0	0.089710	10303	11656	2281-2313	0.500000	60.7
APQ78_gp066_p5	APQ78_gp066	5	TAGTATCCGTGGCTACTGCA	AGGCCAAGCTGTAGAAGGTA	20	20	50.0	50.0	57.9	58.0	11.7	5.0	0.108888	10303	11656	2508-2540	0.500000	60.5
APQ78_gt009-APQ78_gt010_p1	APQ78_gt009-APQ78_gt010	1	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	28183	28706	578-667	1.000000	89.7
APQ78_gt009-APQ78_gt010_p2	APQ78_gt009-APQ78_gt010	2	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	28183	28706	557-646	1.000000	89.2
APQ78_gt009-APQ78_gt010_p3	APQ78_gt009-APQ78_gt010	3	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.367761	28183	28706	554-643	1.000000	89.1
APQ78_gt009-APQ78_gt010_p4	APQ78_gt009-APQ78_gt010	4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.7	58.2	0.0	0.0	0.432442	28183	28706	575-664	1.000000	88.5
APQ78_gt009-APQ78_gt010_p5	APQ78_gt009-APQ78_gt010	5	TGTCCTGAACCACTAGACGA	TTGAACCGATGACTTACGCC	20	20	50.0	50.0	57.7	58.3	0.0	0.0	0.564345	28183	28706	580-669	1.000000	87.5
APQ78_gt010-APQ78_gp057_p1	APQ78_gt010-APQ78_gp057	1	ATGGGGGCATACTTATCCGA	GGAACTGGCTAATCCATGGG	20	20	50.0	55.0	58.0	58.0	0.0	13.0	0.051981	28779	29531	1535-1558	0.666667	78.3
APQ78_gt010-APQ78_gp057_p2	APQ78_gt010-APQ78_gp057	2	ATGGGGGCATACTTATCCGA	TGAACAGATCGAGCAAGCTC	20	20	50.0	50.0	58.0	58.0	0.0	11.9	0.040675	28779	29531	1747-1770	0.666667	77.8
APQ78_gt010-APQ78_gp057_p3	APQ78_gt010-APQ78_gp057	3	ATGGGGGCATACTTATCCGA	AGCTTGGGTGGGGTTAAAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048544	28779	29531	2057-2080	0.666667	76.8
APQ78_gt010-APQ78_gp057_p4	APQ78_gt010-APQ78_gp057	4	ATGGGGGCATACTTATCCGA	AGTTTCTTCAGCTTGGGTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051981	28779	29531	2066-2089	0.666667	76.8
APQ78_gt010-APQ78_gp057_p5	APQ78_gt010-APQ78_gp057	5	ATGGGGGCATACTTATCCGA	GAACAGATCGAGCAAGCTCA	20	20	50.0	50.0	58.0	58.0	0.0	20.7	0.040675	28779	29531	1746-1769	0.500000	63.0
APQ78_gt011-APQ78_gp055_p1	APQ78_gt011-APQ78_gp055	1	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGA	20	20	55.0	40.0	58.1	57.0	9.5	29.0	1.054924	32284	32716	412-509	1.000000	83.6
APQ78_gt011-APQ78_gp055_p2	APQ78_gt011-APQ78_gp055	2	CTATCAACCACTCGGCCATC	TGCAAAAACAGCCAATTGGA	20	20	55.0	40.0	58.1	57.0	2.2	29.0	1.125169	32284	32716	401-498	1.000000	83.0
APQ78_gt011-APQ78_gp055_p3	APQ78_gt011-APQ78_gp055	3	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGAA	20	21	55.0	38.1	58.1	57.7	9.5	29.0	1.331249	32284	32716	412-509	1.000000	81.4
APQ78_gt011-APQ78_gp055_p4	APQ78_gt011-APQ78_gp055	4	CAAGACCGGAGCTATCAACC	AAACAGCCAATTGGAAAGCA	20	20	55.0	40.0	58.1	56.7	9.5	29.0	1.356395	32284	32716	407-504	1.000000	81.1
APQ78_gt011-APQ78_gp055_p5	APQ78_gt011-APQ78_gp055	5	CAAGACCGGAGCTATCAACC	AACAGCCAATTGGAAAGCAA	20	20	55.0	40.0	58.1	56.7	9.5	29.0	1.356395	32284	32716	406-503	1.000000	81.1
APQ78_gp051-APQ78_gt014_p1	APQ78_gp051-APQ78_gt014	1	TCATTGATCTGCGATCGAGG	TTTAACCACTCGGCCATCTC	20	20	50.0	50.0	57.6	57.6	21.6	2.2	0.825477	41269	41767	530-559	1.000000	85.4
APQ78_gp051-APQ78_gt014_p2	APQ78_gp051-APQ78_gt014	2	TCATTGATCTGCGATCGAGG	CTTTAACCACTCGGCCATCT	20	20	50.0	50.0	57.6	57.6	21.6	2.2	0.832139	41269	41767	531-560	1.000000	85.3
APQ78_gp051-APQ78_gt014_p3	APQ78_gp051-APQ78_gt014	3	TCATTGATCTGCGATCGAGG	TTAACCACTCGGCCATCTCT	20	20	50.0	50.0	57.6	58.4	21.6	2.2	0.860384	41269	41767	529-558	1.000000	85.1
APQ78_gp051-APQ78_gt014_p4	APQ78_gp051-APQ78_gt014	4	TCATTGATCTGCGATCGAGG	CCTTTAACCACTCGGCCATC	20	20	50.0	55.0	57.6	58.6	21.6	2.2	1.049361	41269	41767	532-561	1.000000	83.6
APQ78_gp051-APQ78_gt014_p5	APQ78_gp051-APQ78_gt014	5	TCATTGATCTGCGATCGAGG	TAACCACTCGGCCATCTCTC	20	20	50.0	55.0	57.6	58.9	21.6	2.2	1.316775	41269	41767	528-557	1.000000	81.5
APQ78_gp048-APQ78_gp047_p1	APQ78_gp048-APQ78_gp047	1	CTGACCGTAGACTAGAGCCA	GTAAGTCCATCGGTCCACAC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.045789	46960	47623	1558-1596	1.000000	91.6
APQ78_gp048-APQ78_gp047_p2	APQ78_gp048-APQ78_gp047	2	CTGACCGTAGACTAGAGCCA	GGGGACGACCATACTTGTTC	20	20	55.0	55.0	58.0	58.0	0.0	6.2	0.059766	46960	47623	1839-1877	1.000000	91.5
APQ78_gp048-APQ78_gp047_p3	APQ78_gp048-APQ78_gp047	3	CATCCAGTACCGGACCAATG	GTAAGTCCATCGGTCCACAC	20	20	55.0	55.0	58.0	58.0	9.5	0.0	0.052412	46960	47623	941-979	0.833333	84.9
APQ78_gp048-APQ78_gp047_p4	APQ78_gp048-APQ78_gp047	4	CTGACCGTAGACTAGAGCCA	ACGAAACGATCTCTCCAACG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.061276	46960	47623	1990-2028	0.833333	83.8
APQ78_gp048-APQ78_gp047_p5	APQ78_gp048-APQ78_gp047	5	CGAGTCTCTACAGGGCCTAA	GTAAGTCCATCGGTCCACAC	20	20	55.0	55.0	57.9	58.0	5.3	0.0	0.060203	46960	47623	1261-1262	0.500000	60.2
APQ78_gp038-APQ78_gp037_p1	APQ78_gp038-APQ78_gp037	1	ACGGTAGGTACAGCTAGTCC	AGCGCTCTTAGTTCAGTTCG	20	20	55.0	50.0	58.0	58.0	0.0	2.6	0.065916	55636	56496	1662-1777	1.000000	91.5
APQ78_gp038-APQ78_gp037_p2	APQ78_gp038-APQ78_gp037	2	GGTAGGTACAGCTAGTCCGT	AGCGCTCTTAGTTCAGTTCG	20	20	55.0	50.0	58.0	58.0	0.0	2.6	0.065916	55636	56496	1660-1775	1.000000	91.5
APQ78_gp038-APQ78_gp037_p3	APQ78_gp038-APQ78_gp037	3	ATATTCGTTTGGTCGAGGGC	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.1	58.0	0.0	2.6	0.066562	55636	56496	1490-1605	1.000000	91.5
APQ78_gp038-APQ78_gp037_p4	APQ78_gp038-APQ78_gp037	4	TCCTTGTCGGCTCTCTGTAA	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.1	58.0	0.0	2.6	0.096787	55636	56496	1511-1626	1.000000	91.2
APQ78_gp038-APQ78_gp037_p5	APQ78_gp038-APQ78_gp037	5	ATGGAATTCCTTGTCGGCTC	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.0	58.0	24.7	2.6	0.060150	55636	56496	1595-1633	0.666667	76.6
APQ78_gp033-APQ78_gp031_p1	APQ78_gp033-APQ78_gp031	1	TCGCCGTCTACGAGCTATAT	CCTGGAAGCGGAAGAACTAC	20	20	50.0	55.0	57.9	58.0	3.6	0.0	0.101940	59374	60173	1235-1287	0.833333	82.6
APQ78_gp033-APQ78_gp031_p2	APQ78_gp033-APQ78_gp031	2	TCGCCGTCTACGAGCTATAT	TGCCTGTACGGTTGATGATG	20	20	50.0	50.0	57.9	58.0	3.6	0.0	0.113164	59374	60173	1371-1452	0.833333	82.6
APQ78_gp033-APQ78_gp031_p3	APQ78_gp033-APQ78_gp031	3	AGGAGGATTCCCTATTCCCG	TGCCTGTACGGTTGATGATG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.054259	59374	60173	1609-1639	0.666667	73.1
APQ78_gp033-APQ78_gp031_p4	APQ78_gp033-APQ78_gp031	4	AGGAGGATTCCCTATTCCCG	CCTGGAAGCGGAAGAACTAC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.043035	59374	60173	1457-1474	0.666667	72.7
APQ78_gp033-APQ78_gp031_p5	APQ78_gp033-APQ78_gp031	5	AGGAGGATTCCCTATTCCCG	TCCTGGAAGCGGAAGAACTA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.098474	59374	60173	1458-1475	0.666667	72.3
APQ78_gp032_p1	APQ78_gp032	1	ACGACCAGTCAGTTAACAGC	ACCTATTTCACCATAGCGGC	20	20	50.0	50.0	57.8	57.7	8.6	0.0	0.406515	97591	97710	877-1040	1.000000	88.7
APQ78_gp032_p2	APQ78_gp032	2	AGGATTCGAACCTACGACCA	ACCTATTTCACCATAGCGGC	20	20	50.0	50.0	58.2	57.7	15.0	0.0	0.412749	97591	97710	890-1053	1.000000	88.7
APQ78_gp032_p3	APQ78_gp032	3	ACGACCAGTCAGTTAACAGC	GACTGGAGAGCGGGTACTAA	20	20	50.0	55.0	57.8	58.2	8.6	0.0	0.390206	97591	97710	1424-1624	0.833333	82.2
APQ78_gp032_p4	APQ78_gp032	4	AGGATTCGAACCTACGACCA	GACTGGAGAGCGGGTACTAA	20	20	50.0	55.0	58.2	58.2	15.0	0.0	0.396440	97591	97710	1437-1637	0.833333	82.2
APQ78_gp032_p5	APQ78_gp032	5	GGATTCGAACCTACGACCAG	GACTGGAGAGCGGGTACTAA	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.445115	97591	97710	1436-1636	0.833333	81.8
APQ78_gp030-APQ78_gp029_p1	APQ78_gp030-APQ78_gp029	1	AAGGGACTCTTGGAACACCA	CACGATACCAAGGTAAACCCA	20	21	50.0	47.6	58.5	57.7	0.0	2.6	1.819327	62571	63151	564-702	1.000000	77.4
APQ78_gp030-APQ78_gp029_p2	APQ78_gp030-APQ78_gp029	2	AGGGACTCTTGGAACACCAA	CACGATACCAAGGTAAACCCA	20	21	50.0	47.6	58.5	57.7	0.0	2.6	1.819327	62571	63151	563-701	1.000000	77.4
APQ78_gp030-APQ78_gp029_p3	APQ78_gp030-APQ78_gp029	3	AAGGGACTCTTGGAACACCA	ACACGATACCAAGGTAAACCC	20	21	50.0	47.6	58.5	57.7	0.0	2.6	1.820886	62571	63151	565-703	1.000000	77.4
APQ78_gp030-APQ78_gp029_p4	APQ78_gp030-APQ78_gp029	4	AGGGACTCTTGGAACACCAA	ACACGATACCAAGGTAAACCC	20	21	50.0	47.6	58.5	57.7	0.0	2.6	1.820886	62571	63151	564-702	1.000000	77.4
APQ78_gp030-APQ78_gp029_p5	APQ78_gp030-APQ78_gp029	5	GGGACTCTTGGAACACCAAT	CACGATACCAAGGTAAACCCA	20	21	50.0	47.6	57.4	57.7	0.0	2.6	1.924343	62571	63151	562-700	1.000000	76.6
APQ78_gp025_p1	APQ78_gp025	1	TAGCTCCTAGATGGGGAACG	GCTTTCTGGGTCGTCTCAAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.124249	65604	67031	2291-2387	1.000000	91.0
APQ78_gp025_p2	APQ78_gp025	2	TAGCTCCTAGATGGGGAACG	CGTTGAGCCCCTTTTCTTCT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.105642	65604	67031	2198-2211	0.333333	53.6
APQ78_gp025_p3	APQ78_gp025	3	TAGCTCCTAGATGGGGAACG	CTCGTTGAGCCCCTTTTCTT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.105642	65604	67031	2200-2213	0.333333	53.6
APQ78_gp025_p4	APQ78_gp025	4	TAGCTCCTAGATGGGGAACG	TCGTTGAGCCCCTTTTCTTC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.110992	65604	67031	2199-2212	0.333333	53.6
APQ78_gp025_p5	APQ78_gp025	5	TAGCTCCTAGATGGGGAACG	ATCTCGTTGAGCCCCTTTTC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.118544	65604	67031	2202-2215	0.333333	53.5
APQ78_gp018_p1	APQ78_gp018	1	AATTTTCGAGCCCCGCTATT	ACTGAGAGGAATCGACGAGT	20	20	45.0	50.0	57.9	57.9	0.0	3.0	0.169609	71623	73054	2277-2277	0.166667	45.3
APQ78_gp018_p2	APQ78_gp018	2	ATTGGTACCCCCGGTGATTA	ACTGAGAGGAATCGACGAGT	20	20	50.0	50.0	58.1	57.9	10.1	3.0	0.220742	71623	73054	2711-2711	0.166667	44.9
APQ78_gp018_p3	APQ78_gp018	3	TTGGTACCCCCGGTGATTAT	ACTGAGAGGAATCGACGAGT	20	20	50.0	50.0	58.1	57.9	10.1	3.0	0.220742	71623	73054	2710-2710	0.166667	44.9
APQ78_gp018_p4	APQ78_gp018	4	CTGCCGCATTCTCATCAGAT	ACTGAGAGGAATCGACGAGT	20	20	50.0	50.0	58.1	57.9	0.0	3.0	0.236556	71623	73054	2471-2471	0.166667	44.8
APQ78_gp018_p5	APQ78_gp018	5	TGCCGCATTCTCATCAGATC	ACTGAGAGGAATCGACGAGT	20	20	50.0	50.0	58.1	57.9	0.0	3.0	0.241818	71623	73054	2470-2470	0.166667	44.7