primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
cbbX-trnM(cat)_p1	cbbX-trnM(cat)	1	TGTGTAAACTTTCCTCTATTTTGT	CGACCTTGGGCTTATGAGTC	24	20	29.2	55.0	54.7	58.1	0.0	0.0	7.404978	4026	4161	258-258	1.000000	41.6
cbbX-trnM(cat)_p2	cbbX-trnM(cat)	2	TCTTCATCAAGTTTGTGTAAAC	CGACCTTGGGCTTATGAGTC	22	20	31.8	55.0	52.9	58.1	0.0	0.0	7.115775	4026	4161	271-271	1.000000	41.5
cbbX-trnM(cat)_p3	cbbX-trnM(cat)	3	TCTTCATCAAGTTTGTGTAAAC	CTTTGGCTGGACTTGAACCT	22	20	31.8	50.0	52.9	58.0	0.0	0.0	7.070217	4026	4161	292-292	1.000000	41.4
cbbX-trnM(cat)_p4	cbbX-trnM(cat)	4	TGTGTAAACTTTCCTCTATTTTGT	CTTTGGCTGGACTTGAACCT	24	20	29.2	50.0	54.7	58.0	0.0	0.0	7.359421	4026	4161	279-279	1.000000	41.4
cbbX-trnM(cat)_p5	cbbX-trnM(cat)	5	TCTTCATCAAGTTTGTGTAAAC	TTTGGCTGGACTTGAACCTG	22	20	31.8	50.0	52.9	58.3	0.0	0.0	7.365903	4026	4161	291-291	1.000000	41.4
dnaK-trnF(gaa)_p1	dnaK-trnF(gaa)	1	TGAAAGATTTAGAAGAAGAATTAGCT	ACCAACTGAGCTATTTGGGC	26	20	26.9	50.0	55.0	58.2	0.0	19.6	9.177601	27294	27411	182-182	1.000000	44.8
dnaK-trnF(gaa)_p2	dnaK-trnF(gaa)	2	TGAAAGATTTAGAAGAAGAATTAGCT	ACGGATCTTCAGTCCATTGC	26	20	26.9	50.0	55.0	58.0	0.0	0.0	9.046862	27294	27411	205-205	1.000000	44.3
dnaK-trnF(gaa)_p3	dnaK-trnF(gaa)	3	TGAAAGATTTAGAAGAAGAATTAGCT	CGGATCTTCAGTCCATTGCT	26	20	26.9	50.0	55.0	57.7	0.0	0.0	9.339380	27294	27411	204-204	1.000000	44.3
dnaK-trnF(gaa)_p4	dnaK-trnF(gaa)	4	TGAAAGATTTAGAAGAAGAATTAGCT	GACACACGGATCTTCAGTCC	26	20	26.9	55.0	55.0	58.0	0.0	0.0	9.019355	27294	27411	210-210	1.000000	44.2
dnaK-trnF(gaa)_p5	dnaK-trnF(gaa)	5	TGAAAGATTTAGAAGAAGAATTAGC	GACACACGGATCTTCAGTCC	25	20	28.0	55.0	53.7	58.0	0.0	0.0	9.332376	27294	27411	210-210	1.000000	44.2
trnF(gaa)-trnC(gca)_p1	trnF(gaa)-trnC(gca)	1	GCAATGGACTGAAGATCCGT	CCTTACCACTTGGCGATACC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.080373	27485	27494	83-83	1.000000	81.3
trnF(gaa)-trnC(gca)_p2	trnF(gaa)-trnC(gca)	2	GGACTGAAGATCCGTGTGTC	ATAAGTTTAAGGCGGCACCC	20	20	55.0	50.0	58.0	58.2	0.0	13.1	0.243395	27485	27494	136-136	1.000000	79.2
trnF(gaa)-trnC(gca)_p3	trnF(gaa)-trnC(gca)	3	GCAATGGACTGAAGATCCGT	ATAAGTTTAAGGCGGCACCC	20	20	50.0	50.0	58.0	58.2	0.0	13.1	0.270902	27485	27494	141-141	1.000000	79.0
trnF(gaa)-trnC(gca)_p4	trnF(gaa)-trnC(gca)	4	AGCAATGGACTGAAGATCCG	CCTTACCACTTGGCGATACC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.372890	27485	27494	84-84	1.000000	78.9
trnF(gaa)-trnC(gca)_p5	trnF(gaa)-trnC(gca)	5	GGACTGAAGATCCGTGTGTC	TTGCAATCCACTGCCTTACC	20	20	55.0	50.0	58.0	58.5	0.0	0.0	0.457607	27485	27494	91-91	1.000000	78.1
trnN(gtt)-clpN_p1	trnN(gtt)-clpN	1	TGTTCTGGCAACTTTGACCA	ACAATCCCTGGCTTTGTACG	20	20	45.0	50.0	57.9	58.2	0.0	9.8	0.315082	33684	33786	1142-1151	1.000000	81.0
trnN(gtt)-clpN_p2	trnN(gtt)-clpN	2	CCGATTGCTCTACCACTGAG	ACAATCCCTGGCTTTGTACG	20	20	55.0	50.0	57.8	58.2	0.0	9.8	0.394691	33684	33786	692-692	1.000000	80.4
trnN(gtt)-clpN_p3	trnN(gtt)-clpN	3	AAACCTGCGTGTCTAGCTTC	ACAATCCCTGGCTTTGTACG	20	20	50.0	50.0	58.2	58.2	0.0	9.8	0.381759	33684	33786	1304-1313	1.000000	80.1
trnN(gtt)-clpN_p4	trnN(gtt)-clpN	4	AAAAACCTGCGTGTCTAGCT	ACAATCCCTGGCTTTGTACG	20	20	45.0	50.0	57.7	58.2	0.0	9.8	0.434901	33684	33786	1306-1315	1.000000	79.6
trnN(gtt)-clpN_p5	trnN(gtt)-clpN	5	AAAACCTGCGTGTCTAGCTT	ACAATCCCTGGCTTTGTACG	20	20	45.0	50.0	57.7	58.2	0.0	9.8	0.434901	33684	33786	1305-1314	1.000000	79.6
clpN_p1	clpN	1	TGTTCTGGCAACTTTGACCA	ATGCTACACATTCTGCAGCA	20	20	45.0	45.0	57.9	57.9	0.0	9.5	0.254612	33787	34251	1618-1627	1.000000	81.0
clpN_p2	clpN	2	TGTTCTGGCAACTTTGACCA	TGCTACACATTCTGCAGCAT	20	20	45.0	45.0	57.9	57.9	0.0	9.5	0.254612	33787	34251	1617-1626	1.000000	81.0
clpN_p3	clpN	3	TGTTCTGGCAACTTTGACCA	ACAATCCCTGGCTTTGTACG	20	20	45.0	50.0	57.9	58.2	0.0	9.8	0.315082	33787	34251	1142-1151	1.000000	81.0
clpN_p4	clpN	4	CCGATTGCTCTACCACTGAG	ATGCTACACATTCTGCAGCA	20	20	55.0	45.0	57.8	57.9	0.0	9.5	0.334220	33787	34251	1168-1168	1.000000	80.3
clpN_p5	clpN	5	CCGATTGCTCTACCACTGAG	TGCTACACATTCTGCAGCAT	20	20	55.0	45.0	57.8	57.9	0.0	9.5	0.334220	33787	34251	1167-1167	1.000000	80.3
trnI(gau)-ssrA_p1	trnI(gau)-ssrA	1	TCGAACCAGCGATGTTCTTT	TTCAACTCCTACCAGCTCCA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.187994	36964	36987	115-115	1.000000	79.9
trnI(gau)-ssrA_p2	trnI(gau)-ssrA	2	TTCGAACCAGCGATGTTCTT	TTCAACTCCTACCAGCTCCA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.187994	36964	36987	116-116	1.000000	79.9
trnI(gau)-ssrA_p3	trnI(gau)-ssrA	3	TCGAACCAGCGATGTTCTTT	AGGTTCAACTCCTACCAGCT	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.195313	36964	36987	118-118	1.000000	79.8
trnI(gau)-ssrA_p4	trnI(gau)-ssrA	4	TTCGAACCAGCGATGTTCTT	AGGTTCAACTCCTACCAGCT	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.195313	36964	36987	119-119	1.000000	79.8
trnI(gau)-ssrA_p5	trnI(gau)-ssrA	5	CATTAAATGCATCCGGCTGG	TTCAACTCCTACCAGCTCCA	20	20	50.0	50.0	57.6	58.0	4.7	0.0	0.451407	36964	36987	137-137	1.000000	77.6
trnR(ccg)-trnP(tgg)_p1	trnR(ccg)-trnP(tgg)	1	TGAACCCATACTCTCTGGCA	TACCAAACTGCGCTACATCC	20	20	50.0	50.0	58.0	58.0	0.0	9.7	0.067333	37341	37534	273-276	1.000000	84.7
trnR(ccg)-trnP(tgg)_p2	trnR(ccg)-trnP(tgg)	2	CTCTGGCATCGGAAACCATT	TACCAAACTGCGCTACATCC	20	20	50.0	50.0	58.2	58.0	3.2	9.7	0.263362	37341	37534	261-264	1.000000	83.4
trnR(ccg)-trnP(tgg)_p3	trnR(ccg)-trnP(tgg)	3	TGAACCCATACTCTCTGGCA	CTGTGACATTCTGCTCCCAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.238384	37341	37534	304-307	1.000000	82.8
trnR(ccg)-trnP(tgg)_p4	trnR(ccg)-trnP(tgg)	4	TGAACCCATACTCTCTGGCA	AACCTGTGACATTCTGCTCC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.239918	37341	37534	307-310	1.000000	82.7
trnR(ccg)-trnP(tgg)_p5	trnR(ccg)-trnP(tgg)	5	AAAACCACATAACAGCCCCA	TACCAAACTGCGCTACATCC	20	20	45.0	50.0	57.6	58.0	0.0	9.7	0.408767	37341	37534	311-314	1.000000	81.3
rpl27_p1	rpl27	1	CGCTAGCATCTTACGGTTCA	TCAAGACGAGTAAGGTTTGACA	20	22	50.0	40.9	57.8	57.3	16.4	0.0	2.862911	47681	47998	488-488	1.000000	59.1
rpl27_p2	rpl27	2	CGCTAGCATCTTACGGTTCA	TCAAGACGAGTAAGGTTTGACAA	20	23	50.0	39.1	57.8	58.0	16.4	0.0	3.217368	47681	47998	488-488	1.000000	56.2
rpl27_p3	rpl27	3	CGCTAGCATCTTACGGTTCA	TTCAAGACGAGTAAGGTTTGACA	20	23	50.0	39.1	57.8	58.0	16.4	0.0	3.217368	47681	47998	489-489	1.000000	56.2
rpl27_p4	rpl27	4	CGCTAGCATCTTACGGTTCA	CAAGACGAGTAAGGTTTGACA	20	21	50.0	42.9	57.8	55.9	16.4	0.0	3.331426	47681	47998	487-487	1.000000	55.3
rpl27_p5	rpl27	5	ACTTTTGGATCAACAACCGC	TCAAGACGAGTAAGGTTTGACA	20	22	45.0	40.9	57.2	57.3	0.0	0.0	3.452389	47681	47998	514-514	1.000000	54.2
rpl21-eboF_p1	rpl21-eboF	1	TCAACCCAAAATTGTCTACCGA	ATCATGCAACATTTAAAAGATTAG	22	24	40.9	25.0	57.9	52.4	0.0	0.0	11.650819	48459	48913	532-532	1.000000	43.2
rpl21-eboF_p2	rpl21-eboF	2	TCGGTTCAACCCAAAATTGTC	ATCATGCAACATTTAAAAGATTAG	21	24	42.9	25.0	57.3	52.4	0.0	0.0	11.281937	48459	48913	537-537	1.000000	43.1
rpl21-eboF_p3	rpl21-eboF	3	CGGTTCAACCCAAAATTGTCT	ATCATGCAACATTTAAAAGATTAG	21	24	42.9	25.0	57.3	52.4	0.0	0.0	11.289054	48459	48913	536-536	1.000000	43.1
rpl21-eboF_p4	rpl21-eboF	4	TCGGTTCAACCCAAAATTGT	ATCATGCAACATTTAAAAGATTAG	20	24	40.0	25.0	56.1	52.4	0.0	0.0	11.506984	48459	48913	537-537	1.000000	43.1
rpl21-eboF_p5	rpl21-eboF	5	TTCGGTTCAACCCAAAATTGTC	ATCATGCAACATTTAAAAGATTAG	22	24	40.9	25.0	58.0	52.4	0.0	0.0	11.611004	48459	48913	538-538	1.000000	43.1
eboE_p1	eboE	1	GCCTTGGCGTAACAGAGTAA	AGAATTCGTCACGGAGAAGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.091280	50291	51502	1925-1925	1.000000	82.5
eboE_p2	eboE	2	GCCTTGGCGTAACAGAGTAA	GGATAGCCGGAATTGCTGAA	20	20	50.0	50.0	57.9	58.0	0.0	5.0	0.121631	50291	51502	2179-2179	1.000000	82.0
eboE_p3	eboE	3	GAAGATGTGACAGCAGGGAG	AGAATTCGTCACGGAGAAGC	20	20	55.0	50.0	58.0	58.0	2.9	0.0	0.023686	50291	51502	1692-1692	0.500000	59.8
eboE_p4	eboE	4	GAAGATGTGACAGCAGGGAG	GGATAGCCGGAATTGCTGAA	20	20	55.0	50.0	58.0	58.0	2.9	5.0	0.054037	50291	51502	1946-1946	0.500000	59.6
eboE_p5	eboE	5	GTGACAGCAGGGAGTAAAGG	AGAATTCGTCACGGAGAAGC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.102936	50291	51502	1686-1686	0.500000	59.2
eboC_p1	eboC	1	AGCATATTCAAAATTAACCGTAA	TCAGTGCTAAGAGGCCAAGA	23	20	26.1	50.0	52.8	58.4	0.0	5.0	8.560360	52691	53581	1033-1033	1.000000	42.7
eboC_p2	eboC	2	AGCATATTCAAAATTAACCGTAA	CAGTGCTAAGAGGCCAAGAA	23	20	26.1	50.0	52.8	57.5	0.0	5.0	8.684435	52691	53581	1032-1032	1.000000	42.7
eboC_p3	eboC	3	AGCATATTCAAAATTAACCGTAA	TTCAGTGCTAAGAGGCCAAG	23	20	26.1	50.0	52.8	57.5	0.0	5.0	8.684435	52691	53581	1034-1034	1.000000	42.7
eboC_p4	eboC	4	AGCATATTCAAAATTAACCGTAAA	TCAGTGCTAAGAGGCCAAGA	24	20	25.0	50.0	53.6	58.4	0.0	5.0	8.748823	52691	53581	1033-1033	1.000000	42.7
eboC_p5	eboC	5	ATAGCATATTCAAAATTAACCGT	TCAGTGCTAAGAGGCCAAGA	23	20	26.1	50.0	52.6	58.4	0.0	5.0	8.775674	52691	53581	1035-1035	1.000000	42.7
rps6-ccsA_p1	rps6-ccsA	1	ACGTGAATGGGGAGGTTCTA	TATTTCTGGAGCGGTTTGGG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.175799	64142	64191	485-490	1.000000	81.4
rps6-ccsA_p2	rps6-ccsA	2	TACGTGAATGGGGAGGTTCT	TATTTCTGGAGCGGTTTGGG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.175799	64142	64191	486-491	1.000000	81.4
rps6-ccsA_p3	rps6-ccsA	3	ACGTGAATGGGGAGGTTCTA	CAGCCTTTTCGTCTTCAGGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.222848	64142	64191	627-632	1.000000	80.5
rps6-ccsA_p4	rps6-ccsA	4	TACGTGAATGGGGAGGTTCT	CAGCCTTTTCGTCTTCAGGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.222848	64142	64191	628-633	1.000000	80.5
rps6-ccsA_p5	rps6-ccsA	5	AGGCTTACAAGTTCGTGGTG	TATTTCTGGAGCGGTTTGGG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.243830	64142	64191	831-836	1.000000	79.9
ccsA-sufB_p1	ccsA-sufB	1	ACTTGTTGTTGACCCAACCA	CGTTCTTGAAAACGTGAATCG	20	21	45.0	42.9	57.8	56.5	7.3	0.0	2.728121	65101	66042	1082-1087	1.000000	66.0
ccsA-sufB_p2	ccsA-sufB	2	TTGTTGTTGACCCAACCAGT	CGTTCTTGAAAACGTGAATCG	20	21	45.0	42.9	57.8	56.5	7.3	0.0	2.728121	65101	66042	1080-1085	1.000000	66.0
ccsA-sufB_p3	ccsA-sufB	3	CTTGTTGTTGACCCAACCAG	CGTTCTTGAAAACGTGAATCG	20	21	50.0	42.9	57.1	56.5	7.3	0.0	3.373551	65101	66042	1081-1086	1.000000	60.8
ccsA-sufB_p4	ccsA-sufB	4	AGCTACTTGTTGTTGACCCA	CGTTCTTGAAAACGTGAATCG	20	21	45.0	42.9	57.0	56.5	0.0	0.0	3.525543	65101	66042	1086-1091	1.000000	59.6
ccsA-sufB_p5	ccsA-sufB	5	TTGTTGTTGACCCAACCAGT	CACGTAAAGGTTTAACACATCGT	20	23	45.0	39.1	57.8	57.7	7.3	0.0	3.556849	65101	66042	1100-1105	1.000000	59.2
trnG(gcc)-ycf12_p1	trnG(gcc)-ycf12	1	CGTTCAAATCGCGTTACTCG	ATCCCTGTTACCTCCGTGAA	20	20	50.0	50.0	58.0	58.1	5.8	0.0	0.093226	70405	70467	385-385	1.000000	82.0
trnG(gcc)-ycf12_p2	trnG(gcc)-ycf12	2	CGTTCAAATCGCGTTACTCG	AATCCCTGTTACCTCCGTGA	20	20	50.0	50.0	58.0	58.1	5.8	0.0	0.093226	70405	70467	386-386	1.000000	81.9
trnG(gcc)-ycf12_p3	trnG(gcc)-ycf12	3	GGGTTTGGGTTGCTAACTCA	AATCCCTGTTACCTCCGTGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.063309	70405	70467	861-866	1.000000	81.6
trnG(gcc)-ycf12_p4	trnG(gcc)-ycf12	4	GGGTTTGGGTTGCTAACTCA	ATCCCTGTTACCTCCGTGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.063309	70405	70467	860-865	1.000000	81.6
trnG(gcc)-ycf12_p5	trnG(gcc)-ycf12	5	GGGTTTGGGTTGCTAACTCA	CTGTTACCTCCGTGAAAGGG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.105056	70405	70467	856-861	1.000000	81.3
petA-thiS_p1	petA-thiS	1	GTTCAGGCTGCAGAGATGAA	TGTTACTATAGTTGGGGGTGGT	20	22	50.0	45.5	57.9	58.2	20.4	11.4	2.299244	91164	91238	127-127	1.000000	66.6
petA-thiS_p2	petA-thiS	2	AAGTTCAGGCTGCAGAGATG	TGTTACTATAGTTGGGGGTGGT	20	22	50.0	45.5	57.9	58.2	20.4	11.4	2.305140	91164	91238	129-129	1.000000	66.5
petA-thiS_p3	petA-thiS	3	GAAAGTTCAGGCTGCAGAGA	TGTTACTATAGTTGGGGGTGGT	20	22	50.0	45.5	57.8	58.2	20.4	11.4	2.370116	91164	91238	131-131	1.000000	65.9
petA-thiS_p4	petA-thiS	4	GAGAAAGTTCAGGCTGCAGA	TGTTACTATAGTTGGGGGTGGT	20	22	50.0	45.5	57.8	58.2	20.4	11.4	2.370116	91164	91238	133-133	1.000000	65.9
petA-thiS_p5	petA-thiS	5	AGAAAGTTCAGGCTGCAGAG	TGTTACTATAGTTGGGGGTGGT	20	22	50.0	45.5	57.8	58.2	20.4	11.4	2.376192	91164	91238	132-132	1.000000	65.8
psaI-psbJ_p1	psaI-psbJ	1	AGCACGTGCAGAAATAGGTT	CTTGTAGCTTTTGTTGCGGG	20	20	45.0	50.0	57.8	57.9	39.2	0.0	0.235481	104447	104748	1057-1058	1.000000	86.1
psaI-psbJ_p2	psaI-psbJ	2	AAACCAATGATCGACGAGCA	CTTGTAGCTTTTGTTGCGGG	20	20	45.0	50.0	57.9	57.9	19.1	0.0	0.143783	104447	104748	1073-1073	0.500000	58.8
psaI-psbJ_p3	psaI-psbJ	3	AAACCAATGATCGACGAGCA	AGACCGTCAACAAGTTCCAC	20	20	45.0	50.0	57.9	58.1	19.1	0.4	0.143841	104447	104748	1529-1529	0.500000	58.8
psaI-psbJ_p4	psaI-psbJ	4	AAACCAATGATCGACGAGCA	GACCGTCAACAAGTTCCACT	20	20	45.0	50.0	57.9	58.1	19.1	0.0	0.143841	104447	104748	1528-1528	0.500000	58.8
psaI-psbJ_p5	psaI-psbJ	5	AAACCAATGATCGACGAGCA	CTCAATCCTCATATGGCGGG	20	20	45.0	55.0	57.9	57.9	19.1	2.1	0.198312	104447	104748	1714-1714	0.500000	58.4
ycf95_p1	ycf95	1	TTAGTGCGGATGGAGAGACT	AAAACACGCCTTGAAGGACT	20	20	50.0	45.0	57.9	57.9	0.0	16.9	0.198179	125700	126050	911-928	1.000000	80.3
ycf95_p2	ycf95	2	TTAGTGCGGATGGAGAGACT	AAACACGCCTTGAAGGACTT	20	20	50.0	45.0	57.9	57.9	0.0	16.9	0.198179	125700	126050	910-927	1.000000	80.3
ycf95_p3	ycf95	3	TTAGTGCGGATGGAGAGACT	GAAGGACTTGAACCCTCGAC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.227122	125700	126050	899-916	1.000000	80.1
ycf95_p4	ycf95	4	TAGTGCGGATGGAGAGACTC	AAAACACGCCTTGAAGGACT	20	20	55.0	45.0	58.3	57.9	0.0	16.9	0.371244	125700	126050	910-927	1.000000	78.9
ycf95_p5	ycf95	5	TAGTGCGGATGGAGAGACTC	AAACACGCCTTGAAGGACTT	20	20	55.0	45.0	58.3	57.9	0.0	16.9	0.371244	125700	126050	909-926	1.000000	78.9