primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	TGAATATGCTCTGGGACGGA	AAAAAGCGTAGGCCTCGG	20	18	50.0	55.6	58.2	57.7	0.0	9.7	2.508036	7512	8070	731-889	1.000000	67.9
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	GACCAAAACCCGTTGCCTTA	AAAAAGCGTAGGCCTCGG	20	18	50.0	55.6	58.7	57.7	0.0	9.7	2.975758	7512	8070	687-845	1.000000	64.4
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	ATGAATATGCTCTGGGACGG	AAAAAGCGTAGGCCTCGG	20	18	50.0	55.6	57.2	57.7	0.0	9.7	3.143592	7512	8070	732-890	1.000000	62.8
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	GAATATGCTCTGGGACGGAA	AAAAAGCGTAGGCCTCGG	20	18	50.0	55.6	57.1	57.7	0.0	9.7	3.206953	7512	8070	730-888	1.000000	62.3
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	AATATGCTCTGGGACGGAAG	AAAAAGCGTAGGCCTCGG	20	18	50.0	55.6	57.1	57.7	0.0	9.7	3.214977	7512	8070	729-887	1.000000	62.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	35846	36723	949-1053	1.000000	84.3
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	ACTTCCTTTCCTAGTCCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.037693	35846	36723	1011-1115	0.916667	81.3
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CTTCCTTTCCTAGTCCCCCA	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.039125	35846	36723	1010-1114	0.916667	81.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	ACTTCCTTTCCTAGTCCCCC	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.135259	35846	36723	976-1080	0.916667	80.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CTTCCTTTCCTAGTCCCCCA	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.136692	35846	36723	975-1079	0.916667	80.7
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	TCCCGAACGAGATTGGTTTC	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.221690	51373	51822	515-643	1.000000	85.0
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	CCCGAACGAGATTGGTTTCT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.227502	51373	51822	514-642	1.000000	84.9
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	TCCCGAACGAGATTGGTTTC	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.329443	51373	51822	483-611	1.000000	84.3
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	GATCATTTCCGGCTCTAGGC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.2	57.9	0.0	0.0	0.330671	51373	51822	492-620	1.000000	84.2
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	CCCGAACGAGATTGGTTTCT	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.335256	51373	51822	482-610	1.000000	84.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GTCCTTTGTTAATCGGGGCT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.321560	56026	57448	1503-1717	1.000000	84.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	56026	57448	1489-1703	1.000000	84.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GTCCTTTGTTAATCGGGGCT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	0.0	0.0	0.665332	56026	57448	1502-1716	1.000000	81.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	5.2	0.0	0.680379	56026	57448	1488-1702	1.000000	81.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGGTCCTTTGTTAATCGGGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.697675	56026	57448	1505-1719	1.000000	81.5
ndhF_p1	ndhF	1	TGGCTTGGAGCACTAGTACT	TGGGGTCGGTTTAATTTGCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.064617	115589	117808	2907-2991	0.833333	76.5
ndhF_p2	ndhF	2	AGCCCTTTTTCGGTGTTCTT	TGGGGTCGGTTTAATTTGCA	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.070567	115589	117808	2843-2927	0.833333	76.5
ndhF_p3	ndhF	3	AGGCCGAATCTGCACAAAAT	TGGGGTCGGTTTAATTTGCA	20	20	45.0	45.0	58.5	58.0	0.0	0.0	0.453998	115589	117808	2701-2747	0.833333	71.9
ndhF_p4	ndhF	4	TGGCTTGGAGCACTAGTACT	TAACCGGTTAAATGGGGTCG	20	20	50.0	50.0	58.1	57.6	0.0	24.7	0.461332	115589	117808	2919-2980	0.750000	69.2
ndhF_p5	ndhF	5	AGGAAAGAAGGCCGAATCTG	TGGGGTCGGTTTAATTTGCA	20	20	50.0	45.0	57.6	58.0	9.2	0.0	0.413150	115589	117808	2709-2733	0.750000	68.5
ndhF-rpl32_p1	ndhF-rpl32	1	CTTGAGGTCGTGTGAACCAA	GTTCGAAACCTCTCCCCATC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.077484	117809	118599	1644-1881	0.750000	75.4
ndhF-rpl32_p2	ndhF-rpl32	2	CTTGAGGTCGTGTGAACCAA	TCGAAACCTCTCCCCATCTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.099274	117809	118599	1642-1879	0.750000	75.2
ndhF-rpl32_p3	ndhF-rpl32	3	CTTGAGGTCGTGTGAACCAA	TTCGAAACCTCTCCCCATCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.099274	117809	118599	1643-1880	0.750000	75.2
ndhF-rpl32_p4	ndhF-rpl32	4	GGCACCACCAAATACCAGAA	GTTCGAAACCTCTCCCCATC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.107717	117809	118599	1844-2081	0.666667	71.5
ndhF-rpl32_p5	ndhF-rpl32	5	GGCACCACCAAATACCAGAA	TCGAAACCTCTCCCCATCTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.129507	117809	118599	1842-2079	0.666667	71.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGCAAATTAAACCGACCCCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.127479	118759	119654	1397-1621	0.833333	79.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGCAAATTAAACCGACCCCA	AGCAAAGCCCTGGGTAAAAA	20	20	45.0	45.0	58.0	57.9	0.0	15.6	0.098806	118759	119654	1861-2087	0.833333	78.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGCAAATTAAACCGACCCCA	AACCACTCGGATTGAAGAGC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.103806	118759	119654	1929-2155	0.833333	78.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGCAAATTAAACCGACCCCA	TTCCCCAACGAAAAACGACT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.044453	118759	119654	2083-2309	0.750000	75.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CTCCCCCTCCTTAGCTTTCT	TTCCCCAACGAAAAACGACT	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.154829	118759	119654	1930-1930	0.083333	42.1
ccsA_p1	ccsA	1	GATGGGGAGAGGTTTCGAAC	ATGAGTGGCTTTGTTGCTGA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.040115	119817	120785	2270-2323	0.750000	72.5
ccsA_p2	ccsA	2	AAGATGGGGAGAGGTTTCGA	ATGAGTGGCTTTGTTGCTGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.061905	119817	120785	2272-2325	0.750000	72.3
ccsA_p3	ccsA	3	AGATGGGGAGAGGTTTCGAA	ATGAGTGGCTTTGTTGCTGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.061905	119817	120785	2271-2324	0.750000	72.3
ccsA_p4	ccsA	4	GATGGGGAGAGGTTTCGAAC	TATCTTGACGAAATGGGCGG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.077484	119817	120785	2363-2416	0.666667	68.4
ccsA_p5	ccsA	5	AGATGGGGAGAGGTTTCGAA	TATCTTGACGAAATGGGCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.099274	119817	120785	2364-2417	0.666667	68.2
ccsA-ndhD_p1	ccsA-ndhD	1	ATTCGGCAATTGTAGCGTCT	ATGAGTGGCTTTGTTGCTGA	20	20	45.0	45.0	58.0	58.0	7.4	0.0	0.038702	120786	121031	692-695	0.833333	74.9
ccsA-ndhD_p2	ccsA-ndhD	2	CTAACGAAGCTTGGGGATCC	ATGAGTGGCTTTGTTGCTGA	20	20	55.0	45.0	58.0	58.0	13.4	0.0	0.059600	120786	121031	812-815	0.833333	74.4
ccsA-ndhD_p3	ccsA-ndhD	3	AGTCGTTTTTCGTTGGGGAA	ATGAGTGGCTTTGTTGCTGA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.055778	120786	121031	1001-1004	0.750000	70.8
ccsA-ndhD_p4	ccsA-ndhD	4	ATTCGGCAATTGTAGCGTCT	TATCTTGACGAAATGGGCGG	20	20	45.0	50.0	58.0	58.1	7.4	0.0	0.076071	120786	121031	787-788	0.750000	70.3
ccsA-ndhD_p5	ccsA-ndhD	5	AGTCGTTTTTCGTTGGGGAA	TATCTTGACGAAATGGGCGG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.093147	120786	121031	1096-1097	0.666667	66.5
ndhD_p1	ndhD	1	ATTCGGCAATTGTAGCGTCT	CAAATTGCTTCGGCTCCAAG	20	20	45.0	50.0	58.0	58.0	7.4	0.0	0.037478	121032	122534	2127-2135	0.833333	74.6
ndhD_p2	ndhD	2	ATTCGGCAATTGTAGCGTCT	AAGATGTGAATCCGCCTGTC	20	20	45.0	50.0	58.0	58.0	7.4	0.0	0.054014	121032	122534	2083-2091	0.833333	74.5
ndhD_p3	ndhD	3	ATTCGGCAATTGTAGCGTCT	AACAGAAGACTGCGTTGGTT	20	20	45.0	45.0	58.0	58.0	7.4	0.0	0.062562	121032	122534	2107-2115	0.833333	74.5
ndhD_p4	ndhD	4	CTAACGAAGCTTGGGGATCC	CAAATTGCTTCGGCTCCAAG	20	20	55.0	50.0	58.0	58.0	13.4	0.0	0.058375	121032	122534	2247-2255	0.833333	74.4
ndhD_p5	ndhD	5	AGTCGTTTTTCGTTGGGGAA	CAAATTGCTTCGGCTCCAAG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054553	121032	122534	2437-2444	0.750000	70.7
ndhG_p1	ndhG	1	CTAATACATCGGTGGGGCAG	TCGCACAATTTCGAATTCGC	20	20	55.0	45.0	57.8	58.1	0.0	22.9	0.292731	123571	124101	1796-1859	0.833333	75.3
ndhG_p2	ndhG	2	TCTAATACATCGGTGGGGCA	TCGCACAATTTCGAATTCGC	20	20	50.0	45.0	58.2	58.1	0.0	22.9	0.326882	123571	124101	1797-1860	0.833333	75.0
ndhG_p3	ndhG	3	TGAAACAATAGCCAGCCCAA	TCGCACAATTTCGAATTCGC	20	20	45.0	45.0	57.7	58.1	0.0	22.9	0.423855	123571	124101	1411-1474	0.833333	75.0
ndhG_p4	ndhG	4	CTAATACATCGGTGGGGCAG	GCACAATTTCGAATTCGCCT	20	20	55.0	45.0	57.8	57.7	0.0	22.9	0.432404	123571	124101	1794-1857	0.833333	74.2
ndhG_p5	ndhG	5	TCTAATACATCGGTGGGGCA	GCACAATTTCGAATTCGCCT	20	20	50.0	45.0	58.2	57.7	0.0	22.9	0.466556	123571	124101	1795-1858	0.833333	73.9
ndhG-ndhI_p1	ndhG-ndhI	1	ACCTCCTAAGATCAGACCCG	GTTTCGCGATTACCCTGTCT	20	20	55.0	50.0	57.9	58.0	0.0	10.9	0.080699	124102	124637	990-1053	0.833333	79.4
ndhG-ndhI_p2	ndhG-ndhI	2	CGCGGCAGCTACAAAATAAG	GTTTCGCGATTACCCTGTCT	20	20	50.0	50.0	57.9	58.0	7.4	10.9	0.097122	124102	124637	1104-1167	0.833333	78.8
ndhG-ndhI_p3	ndhG-ndhI	3	ACAGTCCAAAGGCGAGAATC	GTTTCGCGATTACCCTGTCT	20	20	50.0	50.0	57.9	58.0	0.0	10.9	0.106575	124102	124637	1205-1268	0.833333	78.2
ndhG-ndhI_p4	ndhG-ndhI	4	TCCAAAGGCGAGAATCCTTG	GTTTCGCGATTACCCTGTCT	20	20	50.0	50.0	57.9	58.0	0.0	10.9	0.119831	124102	124637	1201-1264	0.833333	78.1
ndhG-ndhI_p5	ndhG-ndhI	5	GTCCAAAGGCGAGAATCCTT	GTTTCGCGATTACCCTGTCT	20	20	50.0	50.0	57.9	58.0	0.0	10.9	0.121313	124102	124637	1202-1265	0.833333	78.1
ndhI_p1	ndhI	1	ACCTCCTAAGATCAGACCCG	GCTTTTGGGGGTGGAATTTG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.258207	124638	125132	1644-1707	0.833333	75.9
ndhI_p2	ndhI	2	CGCGGCAGCTACAAAATAAG	GCTTTTGGGGGTGGAATTTG	20	20	50.0	50.0	57.9	57.8	7.4	0.0	0.274630	124638	125132	1758-1821	0.833333	75.5
ndhI_p3	ndhI	3	ACCTCCTAAGATCAGACCCG	AATATGGCTTTTGGGGGTGG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.196518	124638	125132	1650-1706	0.750000	72.7
ndhI_p4	ndhI	4	CGCGGCAGCTACAAAATAAG	AATATGGCTTTTGGGGGTGG	20	20	50.0	50.0	57.9	58.1	7.4	0.0	0.212941	124638	125132	1764-1820	0.750000	72.3
ndhI_p5	ndhI	5	ATCAGACCCGATCCCAGAAA	AATATGGCTTTTGGGGGTGG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.242214	124638	125132	1640-1696	0.750000	72.3
ndhA_p1	ndhA	1	AGACAGGGTAATCGCGAAAC	TCCAACCTTTGAATTGGCGA	20	20	50.0	45.0	58.0	58.0	15.2	0.0	0.017677	125220	127373	2558-2587	0.833333	74.8
ndhA_p2	ndhA	2	AGACAGGGTAATCGCGAAAC	GTGAGAGTTGAAGCCCCAAA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.024714	125220	127373	2524-2553	0.833333	74.8
ndhA_p3	ndhA	3	AGACAGGGTAATCGCGAAAC	TACAACAGGCTCTGGAAGGA	20	20	50.0	50.0	58.0	58.0	15.2	7.1	0.030146	125220	127373	2682-2711	0.833333	74.7
ndhA_p4	ndhA	4	AGACAGGGTAATCGCGAAAC	GTTTATCGGGCCCAATGCTA	20	20	50.0	50.0	58.0	58.0	15.2	24.9	0.030311	125220	127373	2853-2882	0.833333	74.6
ndhA_p5	ndhA	5	AGACAGGGTAATCGCGAAAC	TTTATCGGGCCCAATGCTAC	20	20	50.0	50.0	58.0	58.0	15.2	24.9	0.030311	125220	127373	2852-2881	0.833333	74.6
ndhH_p1	ndhH	1	ATAACCGACCATCTCCGCTA	CAGTTGGGTATTCGAGAGGC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.067554	127376	128572	1610-1626	0.833333	74.3
ndhH_p2	ndhH	2	ATAACCGACCATCTCCGCTA	AAGGGGGTCGGTTGAATTTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.089639	127376	128572	1797-1813	0.833333	74.2
ndhH_p3	ndhH	3	ATAACCGACCATCTCCGCTA	GAAGGGGGTCGGTTGAATTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.089639	127376	128572	1798-1814	0.833333	74.2
ndhH_p4	ndhH	4	GAAGAATTCCTAAGGGCCCG	CAGTTGGGTATTCGAGAGGC	20	20	55.0	55.0	58.0	58.1	18.4	0.0	0.089971	127376	128572	1538-1554	0.833333	74.2
ndhH_p5	ndhH	5	AAATAACCGACCATCTCCGC	CAGTTGGGTATTCGAGAGGC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.109210	127376	128572	1612-1628	0.833333	73.9
rps15-ycf1_p1	rps15-ycf1	1	ACGCGTTACATAGGGCAAAT	TCTGCATCCGCGATTGTAAA	20	20	45.0	45.0	58.0	58.0	5.6	8.2	0.049818	128928	129306	1508-1524	0.833333	75.2
rps15-ycf1_p2	rps15-ycf1	2	ACGCGTTACATAGGGCAAAT	TTCTGCATCCGCGATTGTAA	20	20	45.0	45.0	58.0	58.0	5.6	8.2	0.049818	128928	129306	1509-1525	0.833333	75.2
rps15-ycf1_p3	rps15-ycf1	3	ACGCGTTACATAGGGCAAAT	TTTCTGCATCCGCGATTGTA	20	20	45.0	45.0	58.0	58.0	5.6	8.2	0.049818	128928	129306	1510-1526	0.833333	75.1
rps15-ycf1_p4	rps15-ycf1	4	AACACCATGCATTGATGGGT	TCTGCATCCGCGATTGTAAA	20	20	45.0	45.0	58.1	58.0	8.9	8.2	0.075761	128928	129306	1376-1392	0.833333	75.0
rps15-ycf1_p5	rps15-ycf1	5	ACGCGTTACATAGGGCAAAT	TTGGACTGAGAACAACTGCC	20	20	45.0	50.0	58.0	58.0	5.6	0.0	0.065362	128928	129306	1173-1189	0.583333	64.8
ycf1_p1	ycf1	1	ATTAGTTCCCGCCGAAAGTC	TAAGTGGGCCCTATCGCTTA	20	20	50.0	50.0	58.0	57.9	0.0	25.7	0.111046	132991	133110	910-1165	0.833333	82.2
ycf1_p2	ycf1	2	ATTAGTTCCCGCCGAAAGTC	TCACCTTCTAACGGGTCGAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.176579	132991	133110	872-1127	0.833333	81.6
ycf1_p3	ycf1	3	TTTTCGTGCCTATCGCTCAA	TAAGTGGGCCCTATCGCTTA	20	20	45.0	50.0	57.9	57.9	0.0	25.7	0.178599	132991	133110	1123-1378	0.750000	77.4
ycf1_p4	ycf1	4	TTTTTCGTGCCTATCGCTCA	TAAGTGGGCCCTATCGCTTA	20	20	45.0	50.0	57.9	57.9	0.0	25.7	0.178599	132991	133110	1124-1379	0.750000	77.4
ycf1_p5	ycf1	5	TTCCCACCATACCCTCTTGT	TAAGTGGGCCCTATCGCTTA	20	20	50.0	50.0	57.9	57.9	0.0	25.7	0.144845	132991	133110	861-1116	0.250000	49.3