primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	AGCTCCATCTACAAATGGGT	20	20	55.0	45.0	58.1	56.2	0.0	3.2	1.936678	79	252	217-218	1.000000	76.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	AGCTCCATCTACAAATGGGT	20	20	50.0	45.0	57.9	56.2	0.0	3.2	1.941039	79	252	231-232	1.000000	76.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	AGCTCCATCTACAAATGGGT	20	20	50.0	45.0	57.9	56.2	0.0	3.2	1.942547	79	252	230-231	1.000000	76.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	AGCTCCATCTACAAATGGGT	20	20	50.0	45.0	57.9	56.2	0.0	3.2	1.942547	79	252	232-233	1.000000	76.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	AAGCTCCATCTACAAATGGGT	20	21	55.0	42.9	58.1	57.0	0.0	3.2	2.158020	79	252	218-219	1.000000	74.7
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	GCTTTCGCGTCTCTCTAAAA	CTAGTTCCGGGTTCGAATCC	20	20	45.0	55.0	56.2	57.8	5.8	18.0	2.014514	1315	1528	265-275	1.000000	73.2
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TCGCGTCTCTCTAAAATAGCAG	CTAGTTCCGGGTTCGAATCC	22	20	45.5	55.0	58.0	57.8	5.8	18.0	2.266713	1315	1528	261-271	1.000000	71.3
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	TCGCGTCTCTCTAAAATAGCA	CTAGTTCCGGGTTCGAATCC	21	20	42.9	55.0	57.0	57.8	5.8	18.0	2.270166	1315	1528	261-271	1.000000	71.3
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	GCTTTCGCGTCTCTCTAAAA	ACTCCATCCGACTAGTTCCG	20	20	45.0	55.0	56.2	58.6	5.8	0.0	2.403470	1315	1528	276-286	1.000000	69.9
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	GCTTTCGCGTCTCTCTAAAAT	CTAGTTCCGGGTTCGAATCC	21	20	42.9	55.0	56.7	57.8	5.8	18.0	2.489950	1315	1528	265-275	1.000000	69.4
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9764	9886	203-215	1.000000	70.2
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9764	9886	206-218	1.000000	69.9
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9764	9886	209-221	1.000000	69.4
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9764	9886	212-224	1.000000	69.1
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9764	9886	207-219	1.000000	67.7
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.0	57.9	1.4	21.3	0.104185	13565	14730	1177-1228	1.000000	87.2
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	57.9	57.9	0.0	21.3	0.178077	13565	14730	1233-1284	1.000000	86.3
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	57.9	57.9	0.0	21.3	0.214244	13565	14730	1193-1244	1.000000	86.2
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.2	57.9	1.4	21.3	0.249724	13565	14730	1178-1229	1.000000	86.0
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	20	20	55.0	55.0	58.2	57.9	0.0	21.3	0.256114	13565	14730	1237-1288	1.000000	85.7
pafI-trnS-GGA_p1	pafI-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	45396	46114	808-915	1.000000	78.3
pafI-trnS-GGA_p2	pafI-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	45396	46114	829-936	1.000000	77.7
pafI-trnS-GGA_p3	pafI-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	45396	46114	779-886	1.000000	77.6
pafI-trnS-GGA_p4	pafI-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	45396	46114	781-888	1.000000	77.0
pafI-trnS-GGA_p5	pafI-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	45396	46114	809-916	1.000000	75.9
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	GTTAGTCCCGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	57.7	57.9	0.0	0.0	0.439081	47122	47464	471-472	1.000000	85.3
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	GTTAGTCCCGGTAAAGCTCC	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.546835	47122	47464	439-440	1.000000	85.1
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.3	57.9	0.0	0.0	0.434234	47122	47464	501-502	1.000000	84.8
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	AGATCACTTCCGGCTCTAGG	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.541987	47122	47464	469-470	1.000000	84.5
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TTTTTGAAACGAGGTCCCCG	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.7	57.9	0.0	0.0	0.821836	47122	47464	438-439	1.000000	82.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47538	48335	848-872	1.000000	84.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47538	48335	880-904	1.000000	83.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	57.9	57.8	0.0	15.0	0.338047	47538	48335	866-890	1.000000	82.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ATTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	57.8	57.8	15.5	15.0	0.445800	47538	48335	898-922	1.000000	81.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATGCGATGCTCTAACCTCTG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.527459	47538	48335	850-874	1.000000	81.5
rbcL-accD_p1	rbcL-accD	1	GCTGCTGCTTGTGAAGTATG	ATCGAACCACCATCTTTCCA	20	20	50.0	45.0	57.5	56.8	8.5	0.0	1.711159	57681	58301	686-705	1.000000	72.3
rbcL-accD_p2	rbcL-accD	2	GCTGCTGCTTGTGAAGTATG	TCGAACCACCATCTTTCCAT	20	20	50.0	45.0	57.5	56.8	8.5	0.0	1.711159	57681	58301	685-704	1.000000	72.3
rbcL-accD_p3	rbcL-accD	3	GCTGCTGCTTGTGAAGTATG	AATCGAACCACCATCTTTCCA	20	21	50.0	42.9	57.5	57.6	8.5	0.0	1.972652	57681	58301	687-706	1.000000	70.2
rbcL-accD_p4	rbcL-accD	4	TGCTGCTTGTGAAGTATGGA	ATCGAACCACCATCTTTCCA	20	20	45.0	45.0	57.1	56.8	0.0	0.0	2.028998	57681	58301	684-703	1.000000	69.8
rbcL-accD_p5	rbcL-accD	5	TGCTGCTTGTGAAGTATGGA	TCGAACCACCATCTTTCCAT	20	20	45.0	45.0	57.1	56.8	0.0	0.0	2.028998	57681	58301	683-702	1.000000	69.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGTAAGGGATATTGGGCAGC	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.096773	115097	116017	1025-1052	1.000000	88.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GTAAGGGATATTGGGCAGCC	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.1	58.0	1.3	0.0	0.096773	115097	116017	1024-1051	1.000000	88.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGTAAGGGATATTGGGCAGC	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.098143	115097	116017	1026-1053	1.000000	88.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GTAAGGGATATTGGGCAGCC	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.1	58.0	1.3	0.0	0.098143	115097	116017	1025-1052	1.000000	88.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GTAAGGGATATTGGGCAGCC	CTCGCACTGCTTCCTAAGAG	20	20	55.0	55.0	58.1	58.1	1.3	0.0	0.169024	115097	116017	1045-1072	1.000000	87.7
ccsA-ndhD_p1	ccsA-ndhD	1	GGCGTCTATGGGCTTTCTTA	CAGTTGACAAGGTCGAGGTT	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.855322	117167	117383	367-372	1.000000	78.4
ccsA-ndhD_p2	ccsA-ndhD	2	GGCGTCTATGGGCTTTCTTA	AGTTGACAAGGTCGAGGTTC	20	20	50.0	50.0	57.4	57.5	0.0	0.0	1.142870	117167	117383	366-371	1.000000	76.1
ccsA-ndhD_p3	ccsA-ndhD	3	GGCGTCTATGGGCTTTCTTA	GTTGACAAGGTCGAGGTTCT	20	20	50.0	50.0	57.4	57.5	0.0	0.0	1.142870	117167	117383	365-370	1.000000	76.1
ccsA-ndhD_p4	ccsA-ndhD	4	GGCGTCTATGGGCTTTCTTA	TCAGTTGACAAGGTCGAGGT	20	20	50.0	50.0	57.4	58.6	0.0	0.0	1.204824	117167	117383	368-373	1.000000	75.5
ccsA-ndhD_p5	ccsA-ndhD	5	GGCGTCTATGGGCTTTCTTA	ATCAGTTGACAAGGTCGAGG	20	20	50.0	50.0	57.4	57.2	0.0	0.0	1.365995	117167	117383	369-374	1.000000	74.2
ycf2_p1	ycf2	1	GATACGGGCCTTTTCCACAT	TTCTTTTGTCCCTTCGAGGC	20	20	50.0	50.0	57.9	58.1	0.8	0.0	0.160573	151130	151529	1501-1501	0.500000	58.7
ycf2_p2	ycf2	2	CTCTCCCCCAAAAAGCATGT	TTCTTTTGTCCCTTCGAGGC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.178922	151130	151529	1607-1607	0.500000	58.6
ycf2_p3	ycf2	3	GATACGGGCCTTTTCCACAT	CCGGGATGTGATATGGTTCC	20	20	50.0	55.0	57.9	57.8	0.8	0.0	0.237786	151130	151529	1395-1395	0.500000	58.1
ycf2_p4	ycf2	4	GATACCCGTGACTCGATTGG	TTCTTTTGTCCCTTCGAGGC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.243455	151130	151529	1638-1638	0.500000	58.1
ycf2_p5	ycf2	5	CTCTCCCCCAAAAAGCATGT	CCGGGATGTGATATGGTTCC	20	20	50.0	55.0	58.1	57.8	0.0	0.0	0.256136	151130	151529	1501-1501	0.500000	58.0
rpl23_p1	rpl23	1	GAATGGTTAAAGCGCCCAAC	ACTGTCTACGGTTCGATTGC	20	20	50.0	50.0	58.0	58.0	2.3	0.0	0.017031	153563	153844	587-587	0.500000	59.9
rpl23_p2	rpl23	2	GAATGGTTAAAGCGCCCAAC	CCACAACGATGCTGTCCATA	20	20	50.0	50.0	58.0	58.0	2.3	0.0	0.031965	153563	153844	640-640	0.500000	59.7
rpl23_p3	rpl23	3	GAATGGTTAAAGCGCCCAAC	TCTGTCGGTGCTTGAAACAA	20	20	50.0	45.0	58.0	58.0	2.3	0.0	0.040459	153563	153844	1148-1148	0.500000	59.7
rpl23_p4	rpl23	4	GAATGGTTAAAGCGCCCAAC	TTCTGTCGGTGCTTGAAACA	20	20	50.0	45.0	58.0	58.0	2.3	0.0	0.040459	153563	153844	1149-1149	0.500000	59.7
rpl23_p5	rpl23	5	GATTGCTTGTTGAACCCTGC	ACTGTCTACGGTTCGATTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.078092	153563	153844	904-904	0.500000	59.4
rpl2_p1	rpl2	1	CGCTTCAACCGGGTTATTCT	AACGTGTCACAGCTTACTCC	20	20	50.0	50.0	58.3	57.8	13.0	3.4	0.416235	153863	155338	1610-1610	0.500000	56.7
rpl2_p2	rpl2	2	ATCATTACGCTTCAACCGGG	AACGTGTCACAGCTTACTCC	20	20	50.0	50.0	58.3	57.8	6.3	3.4	0.486703	153863	155338	1617-1617	0.500000	56.1
rpl2_p3	rpl2	3	ACGCTTCAACCGGGTTATTC	AACGTGTCACAGCTTACTCC	20	20	50.0	50.0	58.6	57.8	13.0	3.4	0.704527	153863	155338	1611-1611	0.500000	54.4
rpl2_p4	rpl2	4	TACGCTTCAACCGGGTTATT	AACGTGTCACAGCTTACTCC	20	20	45.0	50.0	57.2	57.8	13.0	3.4	0.916742	153863	155338	1612-1612	0.500000	52.7
rpl2_p5	rpl2	5	TTACGCTTCAACCGGGTTAT	AACGTGTCACAGCTTACTCC	20	20	45.0	50.0	57.2	57.8	13.0	3.4	0.916742	153863	155338	1613-1613	0.500000	52.7