primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	CCGATGGTATGGACGAATCC	TTTGGTCCCGGTGATCAAAG	20	20	55.0	50.0	57.9	58.1	0.0	20.7	0.175999	4258	6255	2091-2162	0.833333	77.3
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	CGATGGTATGGACGAATCCC	TTTGGTCCCGGTGATCAAAG	20	20	55.0	50.0	57.9	58.1	0.0	20.7	0.175999	4258	6255	2090-2161	0.833333	77.3
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	GATGGTATGGACGAATCCCG	TTTGGTCCCGGTGATCAAAG	20	20	55.0	50.0	57.9	58.1	0.0	20.7	0.175999	4258	6255	2089-2160	0.833333	77.3
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	CGATGGTATGGACGAATCCC	CCGGTGATCAAAGGTTCGAA	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.276952	4258	6255	2083-2154	0.833333	76.5
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	GATGGTATGGACGAATCCCG	CCGGTGATCAAAGGTTCGAA	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.276952	4258	6255	2082-2153	0.833333	76.5
petN-psbM_p1	petN-psbM	1	GGCGGCATTAATGACAGTCT	ATTGCTACTGCGCTGTTCAT	20	20	50.0	45.0	58.3	58.3	21.1	25.3	0.588820	26660	27431	915-920	1.000000	77.2
petN-psbM_p2	petN-psbM	2	GGCGGCATTAATGACAGTCT	TATTGCTACTGCGCTGTTCA	20	20	50.0	45.0	58.3	57.6	21.1	25.3	0.710025	26660	27431	916-921	1.000000	76.2
petN-psbM_p3	petN-psbM	3	GGCGGCATTAATGACAGTCT	TTGCTACTGCGCTGTTCATT	20	20	50.0	45.0	58.3	58.5	21.1	25.3	0.808912	26660	27431	914-919	1.000000	75.4
petN-psbM_p4	petN-psbM	4	GGCGGCATTAATGACAGTCT	TGCTTTTATTGCTACTGCGC	20	20	50.0	45.0	58.3	57.5	21.1	0.0	0.874347	26660	27431	922-927	1.000000	74.9
petN-psbM_p5	petN-psbM	5	GGCGGCATTAATGACAGTCT	GCTACTGCGCTGTTCATTCT	20	20	50.0	50.0	58.3	58.6	21.1	25.3	0.967837	26660	27431	912-917	1.000000	74.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTAGTATCCTACCCCCAGG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.1	58.0	0.0	0.0	0.085360	29300	30257	953-1096	1.000000	85.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTAGTATCCTACCCCCAGG	CCATGGCGTTACTCTACCAC	20	20	60.0	55.0	58.1	58.1	0.0	0.0	0.143457	29300	30257	932-1075	1.000000	84.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	29300	30257	902-1045	1.000000	84.6
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	29300	30257	900-1043	1.000000	84.6
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTAGTATCCTACCCCCAGG	TGGCGTTACTCTACCACTGA	20	20	60.0	50.0	58.1	58.1	0.0	0.0	0.165612	29300	30257	929-1072	1.000000	84.4
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTTCTCCTGATGGTTGGGTG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	57.9	57.8	0.0	1.1	0.289634	35165	35856	846-867	1.000000	83.5
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CCCGTTGTTTTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.9	57.8	0.0	1.1	0.304034	35165	35856	859-880	1.000000	83.3
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	35165	35856	849-870	1.000000	82.8
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TTCTCCTGATGGTTGGGTGA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.3	57.8	0.0	1.1	0.428804	35165	35856	845-866	1.000000	82.3
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	CTTCTCCTGATGGTTGGGTG	TAAATACATTGCCTGGGCGG	20	20	55.0	50.0	57.9	58.3	0.0	7.1	0.433447	35165	35856	885-906	1.000000	82.2
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	41223	42058	1004-1039	1.000000	81.5
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	41223	42058	986-1021	1.000000	81.5
psaA-ycf3_p3	psaA-ycf3	3	AGAAATGACCCGGTTTAGCC	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165236	41223	42058	1021-1056	1.000000	81.4
psaA-ycf3_p4	psaA-ycf3	4	AGAAATGACCCGGTTTAGCC	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	0.0	18.7	0.169350	41223	42058	1003-1038	1.000000	81.4
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.214484	41223	42058	1019-1054	1.000000	81.0
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	GATTGGTTTTTGCCGGCTTT	CTCAGAGGTTAGAGCATCGC	20	20	45.0	55.0	58.1	57.9	29.7	0.0	0.255103	45784	46341	717-719	1.000000	78.6
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	GATTGGTTTTTGCCGGCTTT	ATGGTCATCGGTTCGATTCC	20	20	45.0	50.0	58.1	57.8	29.7	0.0	0.362857	45784	46341	685-687	1.000000	77.8
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	GATTGGTTTTTGCCGGCTTT	CAGAGGTTAGAGCATCGCAT	20	20	45.0	50.0	58.1	57.5	29.7	0.0	0.645199	45784	46341	715-717	1.000000	75.5
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	GATTGGTTTTTGCCGGCTTT	TCAGAGGTTAGAGCATCGCA	20	20	45.0	50.0	58.1	58.5	29.7	0.0	0.648034	45784	46341	716-718	1.000000	75.5
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TTGGTTTTTGCCGGCTTTAG	CTCAGAGGTTAGAGCATCGC	20	20	45.0	55.0	57.5	57.9	29.7	0.0	0.650390	45784	46341	715-717	1.000000	75.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46415	47253	872-900	1.000000	80.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46415	47253	904-932	1.000000	79.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAGAAATAGGGAAAGCCGGC	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.2	58.2	21.9	10.2	0.484481	46415	47253	928-956	1.000000	79.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46415	47253	866-894	1.000000	78.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAGAAATAGGGAAAGCCGGC	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	58.2	57.6	21.9	0.0	0.646818	46415	47253	922-950	1.000000	77.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	ATCCACGCCAGAAACGTATC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.115963	48307	49022	851-855	1.000000	81.1
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CCACGCCAGAAACGTATCTT	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.329884	48307	49022	849-853	1.000000	79.4
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	GAGAATCATCCACGCCAGAA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.367821	48307	49022	858-862	1.000000	79.1
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	CATCCACGCCAGAAACGTAT	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.399908	48307	49022	852-856	1.000000	78.9
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.400307	48307	49022	828-832	1.000000	78.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCCAAAACTCCAGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	4.4	0.0	0.552774	50718	51748	1162-1182	1.000000	77.8
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TTTTCGAATCGGGGCCAAAA	AGAAGGTCTACGGTTCGAGT	20	20	45.0	50.0	58.7	57.8	4.4	0.0	0.868027	50718	51748	1172-1192	1.000000	75.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCCAAAACTCCAGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.4	57.5	4.4	0.0	0.896546	50718	51748	1161-1181	1.000000	75.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCCAAAACTCCAGAAAT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	58.4	58.7	4.4	0.0	1.038840	50718	51748	1150-1170	1.000000	73.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCCAAAACTCCAGAAAT	AAGGTCTACGGTTCGAGTCC	20	20	50.0	55.0	58.4	58.8	4.4	0.0	1.183316	50718	51748	1160-1180	1.000000	72.7
accD_p1	accD	1	TCAGAGGAAAGGGGGAAACT	TAAATGGACTTGCCCCATCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.185192	57852	59138	2025-2129	1.000000	81.2
accD_p2	accD	2	TCAGAGGAAAGGGGGAAACT	ACGATTGTGTATCTTGGCCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.172245	57852	59138	2334-2438	1.000000	81.0
accD_p3	accD	3	TCAGAGGAAAGGGGGAAACT	CGGAAGTACTAGGGCCACTA	20	20	50.0	55.0	57.9	57.9	0.0	6.2	0.196828	57852	59138	2238-2342	1.000000	80.9
accD_p4	accD	4	ATTGTTGTGTTGGGTCCACA	TAAATGGACTTGCCCCATCG	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.202837	57852	59138	2244-2348	1.000000	80.8
accD_p5	accD	5	ATTGTTGTGTTGGGTCCACA	ACGATTGTGTATCTTGGCCC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.189889	57852	59138	2553-2657	1.000000	80.6
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	CACTTAGTACCGGTGCTACG	20	20	45.0	55.0	57.9	57.8	5.7	29.3	0.298295	66954	67372	500-507	1.000000	80.8
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CAAAACAAACGCGCTACCAA	CACTTAGTACCGGTGCTACG	20	20	45.0	55.0	58.2	57.8	5.7	29.3	0.355553	66954	67372	501-508	1.000000	80.3
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	TTGTACCCAAAACAAACGCG	CACTTAGTACCGGTGCTACG	20	20	45.0	55.0	57.8	57.8	3.5	29.3	0.374709	66954	67372	508-515	1.000000	80.1
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	AAAACAAACGCGCTACCAAG	AATGTCAACGCATCGGGAAA	20	20	45.0	45.0	57.9	58.5	5.7	0.0	0.584685	66954	67372	570-577	1.000000	78.1
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	AAAACAAACGCGCTACCAAG	ATGTCAACGCATCGGGAAAA	20	20	45.0	45.0	57.9	58.5	5.7	0.0	0.584685	66954	67372	569-576	1.000000	78.1
rpl33-rps18_p1	rpl33-rps18	1	CGACACAATACACCTAACCGA	GGATTTACGAAAGAGCCGCT	21	20	47.6	50.0	57.8	58.3	0.0	0.0	1.567356	68213	68731	635-672	1.000000	70.0
rpl33-rps18_p2	rpl33-rps18	2	TCGACACAATACACCTAACCG	GGATTTACGAAAGAGCCGCT	21	20	47.6	50.0	57.8	58.3	0.0	0.0	1.567356	68213	68731	636-673	1.000000	70.0
rpl33-rps18_p3	rpl33-rps18	3	CGACACAATACACCTAACCGA	TGGATTTACGAAAGAGCCGC	21	20	47.6	50.0	57.8	58.6	0.0	0.0	1.854808	68213	68731	636-673	1.000000	67.7
rpl33-rps18_p4	rpl33-rps18	4	TCGACACAATACACCTAACCG	TGGATTTACGAAAGAGCCGC	21	20	47.6	50.0	57.8	58.6	0.0	0.0	1.854808	68213	68731	637-674	1.000000	67.7
rpl33-rps18_p5	rpl33-rps18	5	CGACACAATACACCTAACCG	GGATTTACGAAAGAGCCGCT	20	20	50.0	50.0	56.3	58.3	0.0	0.0	2.062211	68213	68731	635-672	1.000000	66.1
rps12-clpP_p1	rps12-clpP	1	TGCACCAACGGAAACCATAA	ATGTCAGCCACAGAAGCAAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.031867	70523	70898	597-735	1.000000	91.1
rps12-clpP_p2	rps12-clpP	2	TTGCACCAACGGAAACCATA	ATGTCAGCCACAGAAGCAAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.031867	70523	70898	598-736	1.000000	91.1
rps12-clpP_p3	rps12-clpP	3	TGCACCAACGGAAACCATAA	TATACGGCTCCTAAGGGGTG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.022710	70523	70898	1209-1209	0.166667	46.5
rps12-clpP_p4	rps12-clpP	4	TTGCACCAACGGAAACCATA	TATACGGCTCCTAAGGGGTG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.022710	70523	70898	1210-1210	0.166667	46.5
rps12-clpP_p5	rps12-clpP	5	GATTGGCTGTCTTGCGTTTC	TATACGGCTCCTAAGGGGTG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.036267	70523	70898	979-979	0.166667	46.4
trnN-GUU-rps15_p1	trnN-GUU-rps15	1	ACCCCTTTCGTGTCATTGAC	CGACCACAACAACCGAATTG	20	20	50.0	50.0	58.1	58.0	9.9	0.0	0.138827	107390	107509	331-475	1.000000	87.8
trnN-GUU-rps15_p2	trnN-GUU-rps15	2	ACCCCCGTAACTTCTTCGTA	CGACCACAACAACCGAATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.107681	107390	107509	442-586	1.000000	87.0
trnN-GUU-rps15_p3	trnN-GUU-rps15	3	TCGTAACCCCCGTAACTTCT	CGACCACAACAACCGAATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.107681	107390	107509	447-591	1.000000	87.0
trnN-GUU-rps15_p4	trnN-GUU-rps15	4	GAGGTTTCCTTCGTAACCCC	CGACCACAACAACCGAATTG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.136043	107390	107509	457-601	1.000000	86.7
trnN-GUU-rps15_p5	trnN-GUU-rps15	5	CAATTCCCGTTCTCAACCCA	CGACCACAACAACCGAATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.126750	107390	107509	493-637	1.000000	86.5
ndhD-ccsA_p1	ndhD-ccsA	1	TATCAGGTGAGAGGGTCGAC	GCAGTCTGGGCTAATGAGTC	20	20	55.0	55.0	57.9	58.1	7.1	1.1	0.107487	121441	121692	548-548	1.000000	82.5
ndhD-ccsA_p2	ndhD-ccsA	2	ACTATCAGGTGAGAGGGTCG	GCAGTCTGGGCTAATGAGTC	20	20	55.0	55.0	57.9	58.1	6.6	1.1	0.113305	121441	121692	550-550	1.000000	82.4
ndhD-ccsA_p3	ndhD-ccsA	3	TATCAGGTGAGAGGGTCGAC	TTTGCTACTTCGGGCCTTTT	20	20	55.0	45.0	57.9	58.0	7.1	0.8	0.064526	121441	121692	926-926	1.000000	81.6
ndhD-ccsA_p4	ndhD-ccsA	4	ACTATCAGGTGAGAGGGTCG	TTTGCTACTTCGGGCCTTTT	20	20	55.0	45.0	57.9	58.0	6.6	0.8	0.070344	121441	121692	928-928	1.000000	81.6
ndhD-ccsA_p5	ndhD-ccsA	5	CGGCTTGTTTATCTCGACGA	TTTGCTACTTCGGGCCTTTT	20	20	50.0	45.0	58.1	58.0	0.0	0.8	0.106150	121441	121692	1311-1311	1.000000	80.8
ccsA_p1	ccsA	1	TATCAGGTGAGAGGGTCGAC	TGGAATTGGTAGACACGCTG	20	20	55.0	50.0	57.9	57.9	7.1	0.0	0.147538	121693	122655	1420-1428	1.000000	80.7
ccsA_p2	ccsA	2	TATCAGGTGAGAGGGTCGAC	GTGGAATTGGTAGACACGCT	20	20	55.0	50.0	57.9	57.9	7.1	0.0	0.148994	121693	122655	1421-1429	1.000000	80.7
ccsA_p3	ccsA	3	ACTATCAGGTGAGAGGGTCG	TGGAATTGGTAGACACGCTG	20	20	55.0	50.0	57.9	57.9	6.6	0.0	0.153356	121693	122655	1422-1430	1.000000	80.7
ccsA_p4	ccsA	4	ACTATCAGGTGAGAGGGTCG	GTGGAATTGGTAGACACGCT	20	20	55.0	50.0	57.9	57.9	6.6	0.0	0.154812	121693	122655	1423-1431	1.000000	80.7
ccsA_p5	ccsA	5	TATCAGGTGAGAGGGTCGAC	TAGACACGCTGCTCTTAGGA	20	20	55.0	50.0	57.9	57.9	7.1	0.0	0.178509	121693	122655	1411-1419	1.000000	80.5
trnL-UAG-ndhF_p1	trnL-UAG-ndhF	1	CAGCGTGTCTACCAATTCCA	TTGGAAGTGGAATGAAGGGT	20	20	50.0	45.0	57.9	56.6	0.0	0.0	1.462823	122822	125471	2597-2732	1.000000	74.1
trnL-UAG-ndhF_p2	trnL-UAG-ndhF	2	AGCGTGTCTACCAATTCCAC	TTGGAAGTGGAATGAAGGGT	20	20	50.0	45.0	57.9	56.6	0.0	0.0	1.464279	122822	125471	2596-2731	1.000000	74.1
trnL-UAG-ndhF_p3	trnL-UAG-ndhF	3	TCCTAAGAGCAGCGTGTCTA	TTGGAAGTGGAATGAAGGGT	20	20	50.0	45.0	57.9	56.6	0.0	0.0	1.493794	122822	125471	2606-2741	1.000000	73.9
trnL-UAG-ndhF_p4	trnL-UAG-ndhF	4	AGAAACACAATGCCGCTACT	TTGGAAGTGGAATGAAGGGT	20	20	45.0	45.0	57.8	56.6	0.0	0.0	1.554521	122822	125471	2657-2792	1.000000	73.4
trnL-UAG-ndhF_p5	trnL-UAG-ndhF	5	CTAAGAGCAGCGTGTCTACC	TTGGAAGTGGAATGAAGGGT	20	20	55.0	45.0	57.8	56.6	0.0	0.0	1.565812	122822	125471	2604-2739	1.000000	73.3