primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	99	500	520-520	1.000000	76.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	99	500	534-534	1.000000	76.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	99	500	533-533	1.000000	76.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	99	500	535-535	1.000000	76.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACTTCCCTCTAGACCTAGCG	20	20	55.0	55.0	58.1	57.6	0.0	0.0	0.480626	99	500	473-473	1.000000	76.2
psbA_p1	psbA	1	AAAACGCATTGGTCTCCACT	ATTTGTGTGCTTGGGAGTCC	20	20	45.0	50.0	58.0	58.4	0.0	0.0	0.397921	501	1562	1143-1143	1.000000	76.8
psbA_p2	psbA	2	ACGCATTGGTCTCCACTAAG	ATTTGTGTGCTTGGGAGTCC	20	20	50.0	50.0	57.6	58.4	0.0	0.0	0.765838	501	1562	1140-1140	1.000000	73.9
psbA_p3	psbA	3	CGCATTGGTCTCCACTAAGA	ATTTGTGTGCTTGGGAGTCC	20	20	50.0	50.0	57.3	58.4	0.0	0.0	1.052139	501	1562	1139-1139	1.000000	71.6
psbA_p4	psbA	4	AAAACGCATTGGTCTCCACT	AGAATTTGTGTGCTTGGGAGT	20	21	45.0	42.9	58.0	58.1	0.0	0.0	1.069296	501	1562	1146-1146	1.000000	71.4
psbA_p5	psbA	5	AAAACGCATTGGTCTCCACT	TTTGTGTGCTTGGGAGTCC	20	19	45.0	52.6	58.0	57.9	0.0	0.0	1.128591	501	1562	1142-1142	1.000000	71.0
matK_p1	matK	1	TTGTTAGCTCTTCCGTCAGC	AACACCCTACCTTCAGTTCA	20	20	50.0	45.0	57.9	56.0	0.0	0.0	2.092797	2183	3697	1616-1616	1.000000	63.3
matK_p2	matK	2	TTGTTAGCTCTTCCGTCAGC	ACACCCTACCTTCAGTTCAA	20	20	50.0	45.0	57.9	56.0	0.0	0.0	2.092797	2183	3697	1615-1615	1.000000	63.3
matK_p3	matK	3	TTGTTAGCTCTTCCGTCAGC	ACCCTACCTTCAGTTCAAATCG	20	22	50.0	45.5	57.9	58.1	0.0	0.0	2.141220	2183	3697	1613-1613	1.000000	62.9
matK_p4	matK	4	ATTGCCATCATTTCCACGGT	AACACCCTACCTTCAGTTCA	20	20	45.0	45.0	58.1	56.0	0.0	0.0	2.161208	2183	3697	1585-1585	1.000000	62.7
matK_p5	matK	5	ATTGCCATCATTTCCACGGT	ACACCCTACCTTCAGTTCAA	20	20	45.0	45.0	58.1	56.0	0.0	0.0	2.161208	2183	3697	1584-1584	1.000000	62.7
matK-trnK-UUU_p1	matK-trnK-UUU	1	AAGTCGTGTTGCCGAAATCT	TCGGGTTGCTAACTCAATGG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.228997	3698	4460	855-855	1.000000	78.2
matK-trnK-UUU_p2	matK-trnK-UUU	2	AAGTCGTGTTGCCGAAATCT	CGGGTTGCTAACTCAATGGT	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.299831	3698	4460	854-854	1.000000	77.6
matK-trnK-UUU_p3	matK-trnK-UUU	3	GAAGTCGTGTTGCCGAAATC	TCGGGTTGCTAACTCAATGG	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.335704	3698	4460	856-856	1.000000	77.3
matK-trnK-UUU_p4	matK-trnK-UUU	4	GAAGTCGTGTTGCCGAAATC	CGGGTTGCTAACTCAATGGT	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.406537	3698	4460	855-855	1.000000	76.7
matK-trnK-UUU_p5	matK-trnK-UUU	5	TATAGGAAGTCGTGTTGCCG	TCGGGTTGCTAACTCAATGG	20	20	50.0	50.0	57.4	57.9	0.0	0.0	0.680141	3698	4460	861-861	1.000000	74.6
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCACTTAAAAGCCGAG	TATTTCAAAGAAGGCGGGGG	20	20	50.0	50.0	58.6	57.9	6.4	0.0	0.708347	4498	5369	978-978	1.000000	74.3
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TAGTCGCACTTAAAAGCCGA	TATTTCAAAGAAGGCGGGGG	20	20	45.0	50.0	57.3	57.9	3.0	0.0	0.866500	4498	5369	979-979	1.000000	73.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCGCACTTAAAAGCCGAGTA	TATTTCAAAGAAGGCGGGGG	20	20	45.0	50.0	57.3	57.9	3.0	0.0	0.866500	4498	5369	976-976	1.000000	73.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AGTCGCACTTAAAAGCCGAG	TAAAAAGGGCGCTCAACCAA	20	20	50.0	45.0	58.6	58.3	6.4	11.4	0.876911	4498	5369	1014-1014	1.000000	73.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGTCGCACTTAAAAGCCGAG	TTAAAAAGGGCGCTCAACCA	20	20	50.0	45.0	58.6	58.3	6.4	11.4	0.876911	4498	5369	1015-1015	1.000000	73.0
rps16_p1	rps16	1	TACGAGTTATCCACACCCCC	ACGGGTTCCAAAATAGAGGA	20	20	55.0	45.0	58.5	56.1	0.0	0.0	2.370812	5370	6513	1204-1204	1.000000	61.0
rps16_p2	rps16	2	TACGAGTTATCCACACCCCC	TGTACCGATCCAACAATGACT	20	21	55.0	42.9	58.5	57.0	0.0	0.0	2.484816	5370	6513	1256-1256	1.000000	60.1
rps16_p3	rps16	3	TACGAGTTATCCACACCCCC	ACGGGTTCCAAAATAGAGGAA	20	21	55.0	42.9	58.5	56.9	0.0	0.0	2.593770	5370	6513	1204-1204	1.000000	59.2
rps16_p4	rps16	4	CGAGTTATCCACACCCCCTT	ACGGGTTCCAAAATAGAGGA	20	20	55.0	45.0	59.1	56.1	0.0	0.0	2.949578	5370	6513	1202-1202	1.000000	56.4
rps16_p5	rps16	5	TACGAGTTATCCACACCCCC	ATGTACCGATCCAACAATGACT	20	22	55.0	40.9	58.5	57.5	0.0	0.0	2.999325	5370	6513	1257-1257	1.000000	56.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GGAGGTCAAAATCCACAGCA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	0.0	16.9	0.166401	6514	7149	747-747	1.000000	78.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TGGAGGTCAAAATCCACAGC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	0.0	16.9	0.166401	6514	7149	748-748	1.000000	78.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6514	7149	709-709	1.000000	78.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	0.0	16.9	0.308340	6514	7149	719-719	1.000000	77.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GTGGTGGAGGTCAAAATCCA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.7	58.1	0.0	16.9	0.362466	6514	7149	752-752	1.000000	77.1
trnS-GCU-trnS-CGA_p1	trnS-GCU-trnS-CGA	1	CAATCCGACGCTTTAGTCCA	TGAATCAAACCAAAAGACCCCT	20	22	50.0	40.9	58.0	58.1	0.0	0.0	2.082207	8531	9549	1124-1133	1.000000	64.0
trnS-GCU-trnS-CGA_p2	trnS-GCU-trnS-CGA	2	AATCCGACGCTTTAGTCCAC	TGAATCAAACCAAAAGACCCCT	20	22	50.0	40.9	58.0	58.1	0.0	0.0	2.083595	8531	9549	1123-1132	1.000000	64.0
trnS-GCU-trnS-CGA_p3	trnS-GCU-trnS-CGA	3	AGGAAACGGAAAGAGAGGGA	TGAATCAAACCAAAAGACCCCT	20	22	50.0	40.9	58.0	58.1	0.0	0.0	2.103260	8531	9549	1183-1192	1.000000	63.8
trnS-GCU-trnS-CGA_p4	trnS-GCU-trnS-CGA	4	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCAAAAGACCCCT	20	22	50.0	40.9	58.2	58.1	0.0	0.0	2.228973	8531	9549	1178-1187	1.000000	62.8
trnS-GCU-trnS-CGA_p5	trnS-GCU-trnS-CGA	5	CAATCCGACGCTTTAGTCCA	TGAATCAAACCAAAAGACCCC	20	21	50.0	42.9	58.0	56.6	0.0	0.0	2.398732	8531	9549	1124-1133	1.000000	61.4
trnS-CGA_p1	trnS-CGA	1	CAAAACGCGTCAATGCTCAA	CACTTCAATGATGCATTAAAGTG	20	23	45.0	34.8	58.2	54.9	14.4	17.1	6.357699	9550	10338	978-978	1.000000	40.0
trnS-CGA_p2	trnS-CGA	2	CAAAACGCGTCAATGCTCAA	ACTTCAATGATGCATTAAAGTGA	20	23	45.0	30.4	58.2	54.9	14.4	0.0	6.374829	9550	10338	977-977	1.000000	40.0
trnS-CGA_p3	trnS-CGA	3	CAAAACGCGTCAATGCTCAA	TCACTTCAATGATGCATTAAAGT	20	23	45.0	30.4	58.2	54.9	14.4	0.0	6.374829	9550	10338	979-979	1.000000	40.0
trnS-CGA_p4	trnS-CGA	4	TTTGATTCCGCCGGATTACC	CACTTCAATGATGCATTAAAGTG	20	23	50.0	34.8	58.3	54.9	6.7	17.1	6.439661	9550	10338	932-932	1.000000	40.0
trnS-CGA_p5	trnS-CGA	5	TTTGATTCCGCCGGATTACC	ACTTCAATGATGCATTAAAGTGA	20	23	50.0	30.4	58.3	54.9	6.7	0.0	6.456792	9550	10338	931-931	1.000000	40.0
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	GCACTCTTCATTCTCGTTCCT	20	21	50.0	47.6	58.2	58.0	0.0	0.0	1.185298	30141	30794	809-809	1.000000	70.6
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	ACTGCACTCTTCATTCTCGT	20	20	50.0	45.0	58.2	56.9	0.0	0.0	1.269058	30141	30794	812-812	1.000000	69.9
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	GCACTCTTCATTCTCGTTCCT	20	21	50.0	47.6	57.6	58.0	0.0	0.0	1.384812	30141	30794	748-748	1.000000	69.0
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TGCACTCTTCATTCTCGTTCC	20	21	50.0	47.6	58.2	58.3	0.0	0.0	1.462841	30141	30794	810-810	1.000000	68.4
petN-psbM_p5	petN-psbM	5	ATGGGGAAGAAGTGGACTCT	ACTGCACTCTTCATTCTCGT	20	20	50.0	45.0	57.6	56.9	0.0	0.0	1.468572	30141	30794	751-751	1.000000	68.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CGGATTAGTCGATTCGAATTGG	GGTTCGAATCCGTATAGCCC	22	20	45.5	55.0	57.4	57.9	22.4	0.0	2.707111	53490	54145	723-724	1.000000	58.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CGGATTAGTCGATTCGAATTGG	CGGTTCGAATCCGTATAGCC	22	20	45.5	55.0	57.4	58.3	22.4	22.8	2.936493	53490	54145	724-725	1.000000	56.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CGGATTAGTCGATTCGAATTGG	ACTGCATGTTGGGTCTTTGA	22	20	45.5	45.0	57.4	57.6	22.4	0.0	2.994439	53490	54145	801-802	1.000000	56.1
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	AGGAACTCCTATGAATGTGGA	GGTTCGAATCCGTATAGCCC	21	20	42.9	55.0	55.8	57.9	0.0	0.0	3.320176	53490	54145	752-753	1.000000	53.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CGGATTAGTCGATTCGAATTGG	CGAGAAGGTCTACGGTTCGA	22	20	45.5	55.0	57.4	58.9	22.4	0.0	3.549241	53490	54145	736-737	1.000000	51.7
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	ACGAGAGAGAGATCCACCAG	20	20	45.0	55.0	58.1	57.9	3.3	0.0	0.147048	63370	64331	1079-1080	1.000000	78.9
ycf4-cemA_p2	ycf4-cemA	2	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	63370	64331	1071-1072	1.000000	78.7
ycf4-cemA_p3	ycf4-cemA	3	AAAAAGCTGCTGAATTGGCC	ACGAGAGAGAGATCCACCAG	20	20	45.0	55.0	57.8	57.9	1.4	0.0	0.225698	63370	64331	1093-1094	1.000000	78.3
ycf4-cemA_p4	ycf4-cemA	4	AAAAAGCTGCTGAATTGGCC	GAGATCCACCAGGGCAAAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	63370	64331	1085-1086	1.000000	78.1
ycf4-cemA_p5	ycf4-cemA	5	AAAGCTGCTGAATTGGCCTA	ACGAGAGAGAGATCCACCAG	20	20	45.0	55.0	57.8	57.9	1.4	0.0	0.271987	63370	64331	1091-1092	1.000000	77.9