primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	AAGCGAATGGGCCCTTTTAA	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.1	58.2	25.7	0.0	0.314434	32736	33912	1341-1351	1.000000	78.2
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.349607	32736	33912	1331-1341	1.000000	77.9
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	ATGGGCCCTTTTAACTCAGC	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	25.7	0.0	0.393437	32736	33912	1335-1345	1.000000	77.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	AAGCGAATGGGCCCTTTTAA	CAAAAACGAAACGGTCCCTC	20	20	45.0	50.0	58.1	57.6	25.7	0.0	0.462910	32736	33912	1340-1350	1.000000	77.0
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	AAGCGAATGGGCCCTTTTAA	ATAAGCCGGACCAACCTACA	20	20	45.0	50.0	58.1	58.4	25.7	5.0	0.492946	32736	33912	1358-1368	1.000000	76.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	CTCTAACCGCTGAGCTAAGC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	13.7	0.0	0.158083	48119	48980	906-906	1.000000	78.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CTCTAACCGCTGAGCTAAGC	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	58.2	58.1	13.7	0.0	0.222891	48119	48980	914-914	1.000000	78.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48119	48980	944-944	1.000000	78.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	48119	48980	952-952	1.000000	77.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CAAATGCGATGCTCTAACCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.378359	48119	48980	917-917	1.000000	77.1
trnV-UAC_p1	trnV-UAC	1	TGAGAAAAGTGAAAAGTATACTACA	TGAGAGATCAATCATGTCTTGTCA	25	24	28.0	37.5	54.2	57.7	5.7	0.0	13.094719	54109	54774	862-869	1.000000	42.8
trnV-UAC_p2	trnV-UAC	2	TGAGAAAAGTGAAAAGTATACTACA	AGCAATATTCTCACAGGAAACA	25	22	28.0	36.4	54.2	55.7	5.7	0.0	13.091740	54109	54774	886-893	1.000000	42.7
trnV-UAC_p3	trnV-UAC	3	TGAGAAAAGTGAAAAGTATACTACA	TCTCACAGGAAACATATGAGAGA	25	23	28.0	39.1	54.2	56.5	5.7	10.4	13.328096	54109	54774	878-885	1.000000	42.7
trnV-UAC_p4	trnV-UAC	4	TGAGAAAAGTGAAAAGTATACTACA	AAGCAATATTCTCACAGGAAACA	25	23	28.0	34.8	54.2	56.4	5.7	0.0	13.367566	54109	54774	887-894	1.000000	42.7
trnV-UAC_p5	trnV-UAC	5	TGAGAAAAGTGAAAAGTATACTACAA	AGCAATATTCTCACAGGAAACA	26	22	26.9	36.4	54.9	55.7	5.7	0.0	13.391389	54109	54774	886-893	1.000000	42.7
accD-psaI_p1	accD-psaI	1	AAGGCGTTCTGAGTGAGTTG	ACTAGGCCCACGATAGGTAC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.136382	61567	62463	1003-1033	1.000000	82.4
accD-psaI_p2	accD-psaI	2	AAGGCGTTCTGAGTGAGTTG	CTAGGCCCACGATAGGTACA	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.137799	61567	62463	1002-1032	1.000000	82.4
accD-psaI_p3	accD-psaI	3	AAGGCGTTCTGAGTGAGTTG	GCAGGAAATACTAGGCCCAC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.160216	61567	62463	1012-1042	1.000000	82.1
accD-psaI_p4	accD-psaI	4	AAGGCGTTCTGAGTGAGTTG	AGGAAATACTAGGCCCACGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.298932	61567	62463	1010-1040	1.000000	81.0
accD-psaI_p5	accD-psaI	5	AAGGCGTTCTGAGTGAGTTG	CAGGAAATACTAGGCCCACG	20	20	50.0	55.0	58.1	57.8	0.0	0.0	0.369413	61567	62463	1011-1041	1.000000	80.5
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	AAACCTGCCAAAGACCCAAA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.226982	68256	68611	485-485	1.000000	80.5
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	AAACCTGCCAAAGACCCAAA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.228326	68256	68611	484-484	1.000000	80.5
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	AAACCTGCCAAAGACCCAAA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.235018	68256	68611	496-496	1.000000	80.4
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	AAACCTGCCAAAGACCCAAA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.236351	68256	68611	495-495	1.000000	80.4
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	AAACCTGCCAAAGACCCAAA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.236351	68256	68611	498-498	1.000000	80.4
psaJ-rpl33_p1	psaJ-rpl33	1	ACCCGTGACATTTTGTACCC	TACATACGCGCGCTCTTTAA	20	20	50.0	45.0	58.1	57.8	0.0	32.6	0.291569	68747	69167	1195-1221	1.000000	79.2
psaJ-rpl33_p2	psaJ-rpl33	2	CCCGTGACATTTTGTACCCA	TACATACGCGCGCTCTTTAA	20	20	50.0	45.0	58.1	57.8	0.0	32.6	0.292913	68747	69167	1194-1220	1.000000	79.2
psaJ-rpl33_p3	psaJ-rpl33	3	ACAGGATTTGAACCCGTGAC	TACATACGCGCGCTCTTTAA	20	20	50.0	45.0	58.1	57.8	0.0	32.6	0.299605	68747	69167	1206-1232	1.000000	79.1
psaJ-rpl33_p4	psaJ-rpl33	4	CAGGATTTGAACCCGTGACA	TACATACGCGCGCTCTTTAA	20	20	50.0	45.0	58.1	57.8	0.0	32.6	0.300938	68747	69167	1205-1231	1.000000	79.1
psaJ-rpl33_p5	psaJ-rpl33	5	ATGCGTTGACATTCCCCTTT	TACATACGCGCGCTCTTTAA	20	20	45.0	45.0	58.1	57.8	0.0	32.6	0.268459	68747	69167	681-707	1.000000	78.9
clpP_p1	clpP	1	GGTGCTGCGAAATAGAAGGA	CCCATGAAAATACCCCCTCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.042919	72498	74661	2718-2844	1.000000	83.8
clpP_p2	clpP	2	AGGTGCTGCGAAATAGAAGG	CCCATGAAAATACCCCCTCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.048582	72498	74661	2719-2845	1.000000	83.8
clpP_p3	clpP	3	GTTTCCGCTGCAGATCCTTA	CCCATGAAAATACCCCCTCG	20	20	50.0	55.0	58.0	58.0	20.4	0.0	0.042919	72498	74661	2609-2615	1.000000	80.4
clpP_p4	clpP	4	GTTTCCGCTGCAGATCCTTA	CCCCTGTGATATTCCAACCG	20	20	50.0	55.0	58.0	58.0	20.4	0.0	0.056857	72498	74661	2851-2857	1.000000	80.3
clpP_p5	clpP	5	GTTTCCGCTGCAGATCCTTA	CTTCCGCCTTTCAGTTCAGT	20	20	50.0	50.0	58.0	58.1	20.4	0.0	0.139940	72498	74661	2535-2541	1.000000	79.7
psbT-psbN_p1	psbT-psbN	1	GGTTCCGACTAAAAGGGCAA	GATCCATTCGAGGAACACGG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.527836	76903	76968	118-119	1.000000	80.5
psbT-psbN_p2	psbT-psbN	2	GGTTCCGACTAAAAGGGCAA	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	58.1	57.7	0.0	0.0	0.384017	76903	76968	166-167	1.000000	80.3
psbT-psbN_p3	psbT-psbN	3	AAGGTTCCGACTAAAAGGGC	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.8	57.7	0.0	0.0	0.480004	76903	76968	168-169	1.000000	79.5
psbT-psbN_p4	psbT-psbN	4	GGTTCCGACTAAAAGGGCAA	GTTTTACCGGGTACGCCTTA	20	20	50.0	50.0	58.1	57.6	0.0	6.3	0.478199	76903	76968	174-175	1.000000	79.4
psbT-psbN_p5	psbT-psbN	5	GGTTCCGACTAAAAGGGCAA	TAAGTTTTACCGGGTACGCC	20	20	50.0	50.0	58.1	57.6	0.0	6.3	0.478199	76903	76968	177-178	1.000000	79.3
rpl16_p1	rpl16	1	GTGGGGAACTGCTTCTTTGA	AAAAGGCATCGACCTGATCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062866	83964	85532	2506-2637	1.000000	89.9
rpl16_p2	rpl16	2	CGCACCAAAAACTCGAGTTC	AAAAGGCATCGACCTGATCC	20	20	50.0	50.0	58.0	58.0	29.8	0.0	0.062840	83964	85532	2662-2793	1.000000	89.8
rpl16_p3	rpl16	3	CGCACCAAAAACTCGAGTTC	CGCAGGACGTATTGAAGGAA	20	20	50.0	50.0	58.0	58.0	29.8	3.2	0.023300	83964	85532	2359-2359	0.500000	59.8
rpl16_p4	rpl16	4	GTGGGGAACTGCTTCTTTGA	CGCAGGACGTATTGAAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	3.2	0.023326	83964	85532	2203-2203	0.500000	59.8
rpl16_p5	rpl16	5	CGCACCAAAAACTCGAGTTC	GCAAACCATTCGAGCGAAAA	20	20	50.0	45.0	58.0	58.0	29.8	9.5	0.063605	83964	85532	2293-2293	0.500000	59.5
rpl16-rps3_p1	rpl16-rps3	1	TTCCTGAGCCAATCTCCTCA	AAAAGGCATCGACCTGATCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.087254	85533	85731	1192-1323	1.000000	91.3
rpl16-rps3_p2	rpl16-rps3	2	TTCCTGAGCCAATCTCCTCA	CAAGCCAAAGCCATCATTCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.125859	85533	85731	1349-1480	1.000000	91.0
rpl16-rps3_p3	rpl16-rps3	3	TTCCTGAGCCAATCTCCTCA	CGCAGGACGTATTGAAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	3.2	0.047714	85533	85731	889-889	0.500000	59.6
rpl16-rps3_p4	rpl16-rps3	4	TTCCTGAGCCAATCTCCTCA	GCAAACCATTCGAGCGAAAA	20	20	50.0	45.0	58.0	58.0	0.0	9.5	0.088019	85533	85731	823-823	0.500000	59.3
rpl16-rps3_p5	rpl16-rps3	5	TTCCTGAGCCAATCTCCTCA	GGAAACGAAATTGCACGTGT	20	20	50.0	45.0	58.0	57.9	0.0	18.4	0.157042	85533	85731	873-873	0.500000	58.7
rps3_p1	rps3	1	TTCCTGAGCCAATCTCCTCA	AATTGATCAGATTCGGGGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056249	85732	86397	1803-1934	1.000000	90.8
rps3_p2	rps3	2	TTCCTGAGCCAATCTCCTCA	TAGTCAAGCCGAAGTGAACG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.103457	85732	86397	1650-1781	1.000000	90.8
rps3_p3	rps3	3	TTCCTGAGCCAATCTCCTCA	CGCACCTACTCTGACTTTCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.045462	85732	86397	1994-2125	1.000000	90.6
rps3_p4	rps3	4	TTCCTGAGCCAATCTCCTCA	GCACCTACTCTGACTTTCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.045462	85732	86397	1993-2124	1.000000	90.6
rps3_p5	rps3	5	TTCCTGAGCCAATCTCCTCA	TCCCGGGCATCTACCATTAT	20	20	50.0	50.0	58.0	58.0	0.0	18.5	0.081994	85732	86397	2113-2244	1.000000	90.2
rpl32_p1	rpl32	1	AGAAGTCTTACCCAGTTAACT	GAGCGATTCGTTTGCATTCA	21	20	38.1	45.0	53.5	57.5	1.1	8.0	6.000809	117633	117755	244-245	1.000000	40.7
rpl32_p2	rpl32	2	AGAAGTCTTACCCAGTTAACT	TGAGCGATTCGTTTGCATTC	21	20	38.1	45.0	53.5	57.5	1.1	8.0	6.000809	117633	117755	245-246	1.000000	40.7
rpl32_p3	rpl32	3	TGATCAATAGAAGTCTTACCCAGT	GAGCGATTCGTTTGCATTCA	24	20	37.5	45.0	56.9	57.5	0.0	8.0	5.649233	117633	117755	252-253	1.000000	40.6
rpl32_p4	rpl32	4	TGATCAATAGAAGTCTTACCCAGT	TGAGCGATTCGTTTGCATTC	24	20	37.5	45.0	56.9	57.5	0.0	8.0	5.649233	117633	117755	253-254	1.000000	40.6
rpl32_p5	rpl32	5	TGATCAATAGAAGTCTTACCCAGTT	GAGCGATTCGTTTGCATTCA	25	20	36.0	45.0	57.5	57.5	0.0	8.0	6.022096	117633	117755	252-253	1.000000	40.6
ccsA-ndhD_p1	ccsA-ndhD	1	CAATTGTGGCTTCTACGGGA	CCTATCAGTTGACAGGGTAGA	20	21	50.0	47.6	57.9	55.9	0.0	0.0	3.219695	119684	119885	364-364	1.000000	55.6
ccsA-ndhD_p2	ccsA-ndhD	2	ACTCTGCAATTGTGGCTTCT	CCTATCAGTTGACAGGGTAGA	20	21	45.0	47.6	57.7	55.9	7.4	0.0	3.394891	119684	119885	370-370	1.000000	54.1
ccsA-ndhD_p3	ccsA-ndhD	3	CAATTGTGGCTTCTACGGGA	CCTATCAGTTGACAGGGTAGAA	20	22	50.0	45.5	57.9	56.6	0.0	0.0	3.469803	119684	119885	364-364	1.000000	53.6
ccsA-ndhD_p4	ccsA-ndhD	4	TGTGGCTTCTACGGGATTTC	CCTATCAGTTGACAGGGTAGA	20	21	50.0	47.6	57.6	55.9	0.0	0.0	3.508335	119684	119885	360-360	1.000000	53.3
ccsA-ndhD_p5	ccsA-ndhD	5	CTGCAATTGTGGCTTCTACG	CCTATCAGTTGACAGGGTAGA	20	21	50.0	47.6	57.5	55.9	7.4	0.0	3.644276	119684	119885	367-367	1.000000	52.2
ndhD_p1	ndhD	1	TCTCAAACAAAAACTCCAAACGT	TCGAGTATGGGTTTTTCTGTCC	23	22	34.8	45.5	57.6	58.1	0.0	0.0	5.445664	119886	121388	1649-1649	1.000000	40.0
ndhD_p2	ndhD	2	TGGTTCTCAAACAAAAACTCCA	TCGAGTATGGGTTTTTCTGTCC	22	22	36.4	45.5	56.6	58.1	0.0	0.0	5.480585	119886	121388	1653-1653	1.000000	40.0
ndhD_p3	ndhD	3	TCTCAAACAAAAACTCCAAACGT	TGGGTTTTTCTGTCCAAAGTG	23	21	34.8	42.9	57.6	56.8	0.0	0.0	5.541368	119886	121388	1642-1642	1.000000	40.0
ndhD_p4	ndhD	4	TCTCAAACAAAAACTCCAAACGT	GGGTTTTTCTGTCCAAAGTGT	23	21	34.8	42.9	57.6	56.8	0.0	0.0	5.543436	119886	121388	1641-1641	1.000000	40.0
ndhD_p5	ndhD	5	TGGTTCTCAAACAAAAACTCCA	TGGGTTTTTCTGTCCAAAGTG	22	21	36.4	42.9	56.6	56.8	0.0	0.0	5.576289	119886	121388	1646-1646	1.000000	40.0
ndhD-psaC_p1	ndhD-psaC	1	ATAAAATGAGAGAGCCCGCG	TATGGCATGAGACAACTCGC	20	20	50.0	50.0	58.1	58.1	0.0	11.0	0.188946	121389	121523	243-243	1.000000	82.1
ndhD-psaC_p2	ndhD-psaC	2	CGCGAATATCGGCAAAACAA	TATGGCATGAGACAACTCGC	20	20	45.0	50.0	57.8	58.1	0.0	11.0	0.226767	121389	121523	227-227	1.000000	82.1
ndhD-psaC_p3	ndhD-psaC	3	ATAAAATGAGAGAGCCCGCG	CGGATTTCCTGAGTGTACGG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.206349	121389	121523	270-270	1.000000	81.6
ndhD-psaC_p4	ndhD-psaC	4	CGCGAATATCGGCAAAACAA	CGGATTTCCTGAGTGTACGG	20	20	45.0	55.0	57.8	58.1	0.0	0.0	0.244171	121389	121523	254-254	1.000000	81.5
ndhD-psaC_p5	ndhD-psaC	5	ATAAAATGAGAGAGCCCGCG	GAAGACTGCGTGGGTTGTAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.255222	121389	121523	313-313	1.000000	80.8