primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCGGTCTTCAAAACTT	AAGGAGGGCAGAGGTTTTTG	20	20	45.0	50.0	58.1	58.0	6.1	0.0	0.051611	3936	4499	741-788	0.875000	82.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	ACTCGTGGAAAAACCCTTCC	AAGGAGGGCAGAGGTTTTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024020	3936	4499	1044-1091	0.875000	81.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	ACTCGTGGAAAAACCCTTCC	TGCCGAAGAGAAGGAAGAGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.098213	3936	4499	1181-1228	0.875000	80.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	ACTCGTGGAAAAACCCTTCC	CCACAGTGTATCACGCAAGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.101792	3936	4499	1729-1729	0.250000	49.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	ACTCGTGGAAAAACCCTTCC	TCCACAGTGTATCACGCAAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.101792	3936	4499	1730-1730	0.250000	49.2
atpF-atpH_p1	atpF-atpH	1	AGAAAGAGTACCATGCTGCG	GCTGTAGAAGGTATCGCGAG	20	20	50.0	55.0	58.0	58.0	0.0	16.7	0.055063	12381	12772	907-941	1.000000	87.2
atpF-atpH_p2	atpF-atpH	2	AGAAAGAGTACCATGCTGCG	TGATTTCTGCTGCTTCCGTT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.119516	12381	12772	999-1033	1.000000	86.3
atpF-atpH_p3	atpF-atpH	3	AGGAAAAAGTACAGCTGGGC	GCTGTAGAAGGTATCGCGAG	20	20	50.0	55.0	58.1	58.0	19.9	16.7	0.128269	12381	12772	715-749	0.875000	82.4
atpF-atpH_p4	atpF-atpH	4	CAATACCCCGAGCACTACAC	GCTGTAGAAGGTATCGCGAG	20	20	55.0	55.0	58.1	58.0	0.0	16.7	0.103394	12381	12772	656-672	0.875000	78.7
atpF-atpH_p5	atpF-atpH	5	AGAAAGAGTACCATGCTGCG	TTGTGCGTTGCGTTGAATTT	20	20	50.0	40.0	58.0	58.1	0.0	0.0	0.101102	12381	12772	1319-1342	0.625000	67.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	30194	31126	959-1041	1.000000	90.3
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	30194	31126	957-1039	1.000000	90.3
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AGAGATGTCCTGAACCGCTA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.231619	30194	31126	956-1038	1.000000	90.1
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGATGTCCTGAACCGCTAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.231619	30194	31126	954-1036	1.000000	90.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GATGTCCTGAACCGCTAGAC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.275420	30194	31126	956-1038	1.000000	89.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCAATGAAGCAACAGCTGGT	AGCCTGACAAATGGTTTGGT	20	20	45.0	45.0	58.0	57.9	19.9	7.3	0.101137	49227	49949	1789-1988	1.000000	86.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	AAGGGCAACTCAACGGAATT	AGCCTGACAAATGGTTTGGT	20	20	45.0	45.0	58.0	57.9	0.0	7.3	0.091234	49227	49949	1672-1881	1.000000	86.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTAAGACCATTCCAAGGCCC	AGCCTGACAAATGGTTTGGT	20	20	55.0	45.0	57.9	57.9	0.0	7.3	0.145203	49227	49949	1601-1810	1.000000	85.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	AAGGGCAACTCAACGGAATT	ATGGTTTGGTCCGAATCAGG	20	20	45.0	50.0	58.0	57.9	0.0	10.7	0.133394	49227	49949	1662-1861	0.750000	74.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCAATGAAGCAACAGCTGGT	ATGGTTTGGTCCGAATCAGG	20	20	45.0	50.0	58.0	57.9	19.9	10.7	0.143297	49227	49949	1779-1978	0.750000	74.6
rbcL-accD_p1	rbcL-accD	1	TTGAATGCTACTGCGGGTAC	AAACCAGACAAACTCGTGCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.056929	55199	55902	1548-1567	0.625000	69.0
rbcL-accD_p2	rbcL-accD	2	TTGAATGCTACTGCGGGTAC	AACCAGACAAACTCGTGCTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.056929	55199	55902	1547-1566	0.625000	68.9
rbcL-accD_p3	rbcL-accD	3	ATGAAATTATCCGTGCGGCT	TCTTTGTGCGAAACCAGACA	20	20	45.0	45.0	58.0	58.0	5.6	0.0	0.063940	55199	55902	951-970	0.375000	58.7
rbcL-accD_p4	rbcL-accD	4	AATGAGGGACGTGATCTTGC	TCTTTGTGCGAAACCAGACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.061097	55199	55902	982-1001	0.375000	58.6
rbcL-accD_p5	rbcL-accD	5	TTGAATGCTACTGCGGGTAC	TCTTTGTGCGAAACCAGACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.049800	55199	55902	1558-1577	0.375000	57.3
accD_p1	accD	1	TGGGCTAGATGGAGGTCAAA	CCAACAGCGATTCTGAGTCA	20	20	50.0	50.0	58.0	57.9	0.0	1.0	0.119195	55903	57639	1989-2274	0.500000	66.8
accD_p2	accD	2	TTGGGCTAGATGGAGGTCAA	CCAACAGCGATTCTGAGTCA	20	20	50.0	50.0	58.0	57.9	0.0	1.0	0.119195	55903	57639	1990-2275	0.500000	66.8
accD_p3	accD	3	TTTGGGCTAGATGGAGGTCA	CCAACAGCGATTCTGAGTCA	20	20	50.0	50.0	58.0	57.9	0.0	1.0	0.119195	55903	57639	1991-2276	0.500000	66.8
accD_p4	accD	4	TGGGCTAGATGGAGGTCAAA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.138123	55903	57639	2396-2690	0.500000	66.3
accD_p5	accD	5	TTGGGCTAGATGGAGGTCAA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.138123	55903	57639	2397-2691	0.500000	66.3
accD-psaI_p1	accD-psaI	1	CGCCTATATTGCATTTGCGG	TTGTTTTTCGAGACCCCGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.073489	57640	58219	1363-1430	0.875000	84.2
accD-psaI_p2	accD-psaI	2	GGTGACAGCCAGTTTTGGTA	TTGTTTTTCGAGACCCCGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.065591	57640	58219	1414-1481	0.875000	84.1
accD-psaI_p3	accD-psaI	3	TACTACTGGTGGGGTGACAG	TTGTTTTTCGAGACCCCGTT	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.108230	57640	58219	1426-1493	0.875000	83.6
accD-psaI_p4	accD-psaI	4	AGTATGGGATCCGTAGTGGG	TTGTTTTTCGAGACCCCGTT	20	20	55.0	45.0	58.0	58.0	5.5	0.0	0.052373	57640	58219	1635-1702	0.875000	83.1
accD-psaI_p5	accD-psaI	5	TCCTGTAGCAATGGGGGTTA	TTGTTTTTCGAGACCCCGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.067223	57640	58219	1678-1745	0.875000	82.8
pafII-cemA_p1	pafII-cemA	1	AGGGGGCTATTCCTTTGACT	TTCCTATACGCGGGTTTTGG	20	20	50.0	50.0	58.0	58.0	0.0	11.5	0.039924	59330	59758	793-817	1.000000	88.8
pafII-cemA_p2	pafII-cemA	2	AGGGGGCTATTCCTTTGACT	ATTCATAGCCGCGGATCATC	20	20	50.0	50.0	58.0	58.0	0.0	33.5	0.028656	59330	59758	1251-1267	0.625000	66.4
pafII-cemA_p3	pafII-cemA	3	AGGGGGCTATTCCTTTGACT	GATTCATAGCCGCGGATCAT	20	20	50.0	50.0	58.0	58.0	0.0	33.5	0.028656	59330	59758	1252-1268	0.625000	66.4
pafII-cemA_p4	pafII-cemA	4	AACGGGGTCTCGAAAAACAA	ATTCATAGCCGCGGATCATC	20	20	45.0	50.0	58.0	58.0	0.0	33.5	0.056987	59330	59758	1658-1674	0.625000	66.1
pafII-cemA_p5	pafII-cemA	5	AACGGGGTCTCGAAAAACAA	GATTCATAGCCGCGGATCAT	20	20	45.0	50.0	58.0	58.0	0.0	33.5	0.056987	59330	59758	1659-1675	0.625000	66.1
infA-rps8_p1	infA-rps8	1	CACTTGACCCCGTACATCTG	TGGGTCTCACGCATATACCT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.092285	79049	79159	1370-1407	1.000000	86.4
infA-rps8_p2	infA-rps8	2	CACTTGACCCCGTACATCTG	GATCAGTCGACCCGGTTTAC	20	20	55.0	55.0	58.0	58.1	0.0	13.0	0.091551	79049	79159	997-1023	1.000000	86.2
infA-rps8_p3	infA-rps8	3	CACTTGACCCCGTACATCTG	GAAGGAACTCGCGTTTTTGG	20	20	55.0	50.0	58.0	58.0	0.0	5.8	0.042559	79049	79159	1543-1557	0.875000	78.5
infA-rps8_p4	infA-rps8	4	GGCAGAAGACCAAGAAACCA	GAAGGAACTCGCGTTTTTGG	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.051158	79049	79159	1561-1575	0.875000	78.4
infA-rps8_p5	infA-rps8	5	CGAACCCGGAACATACCATT	GAAGGAACTCGCGTTTTTGG	20	20	50.0	50.0	58.0	58.0	0.7	5.8	0.065095	79049	79159	889-896	0.750000	73.9
rpl22-rps19_p1	rpl22-rps19	1	CGTAGGAACGTCCACGAATT	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	58.0	58.0	6.0	29.9	0.016504	83135	83225	747-759	1.000000	85.8
rpl22-rps19_p2	rpl22-rps19	2	TTGTCCCATAGTCCCCCATT	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	58.0	58.0	0.0	29.9	0.008803	83135	83225	1108-1120	1.000000	85.2
rpl22-rps19_p3	rpl22-rps19	3	TTTGTCCCATAGTCCCCCAT	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	58.0	58.0	0.0	29.9	0.008803	83135	83225	1109-1121	1.000000	85.2
rpl22-rps19_p4	rpl22-rps19	4	TTGTCCCATAGTCCCCCATT	GAGGAGTAAGCTGTGACACG	20	20	50.0	55.0	58.0	58.0	0.0	0.2	0.021629	83135	83225	845-856	0.875000	77.1
rpl22-rps19_p5	rpl22-rps19	5	TTTGTCCCATAGTCCCCCAT	GAGGAGTAAGCTGTGACACG	20	20	50.0	55.0	58.0	58.0	0.0	0.2	0.021629	83135	83225	846-857	0.875000	77.1
trnN-GUU-trnL-UAG_p1	trnN-GUU-trnL-UAG	1	CTACCACTGAGCTACTGAGGA	AGCGTGTCTACCGATTTCAC	21	20	52.4	50.0	58.0	58.0	11.2	0.0	1.013942	108825	110120	1180-1346	0.875000	78.9
trnN-GUU-trnL-UAG_p2	trnN-GUU-trnL-UAG	2	TCTACCACTGAGCTACTGAGG	AGCGTGTCTACCGATTTCAC	21	20	52.4	50.0	58.0	58.0	0.0	0.0	1.013942	108825	110120	1181-1347	0.875000	78.9
trnN-GUU-trnL-UAG_p3	trnN-GUU-trnL-UAG	3	CTACCACTGAGCTACTGAGGA	CAGCGTGTCTACCGATTTCA	21	20	52.4	50.0	58.0	58.0	11.2	0.0	1.015312	108825	110120	1181-1347	0.875000	78.9
trnN-GUU-trnL-UAG_p4	trnN-GUU-trnL-UAG	4	TCTACCACTGAGCTACTGAGG	CAGCGTGTCTACCGATTTCA	21	20	52.4	50.0	58.0	58.0	0.0	0.0	1.015312	108825	110120	1182-1348	0.875000	78.9
trnN-GUU-trnL-UAG_p5	trnN-GUU-trnL-UAG	5	CTACCACTGAGCTACTGAGGA	TCCTAAGAGCAGCGTGTCTA	21	20	52.4	50.0	58.0	57.9	11.2	0.0	1.130389	108825	110120	1190-1356	0.875000	78.0
ndhG-ndhI_p1	ndhG-ndhI	1	AACACCACTCCTAGACCTCC	GCTGCGAGATACATTGGTCA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.120046	114713	115077	890-901	1.000000	88.6
ndhG-ndhI_p2	ndhG-ndhI	2	AACACCACTCCTAGACCTCC	TGTGAAGTATGTGTTCGCGT	20	20	55.0	45.0	58.1	57.9	0.0	5.8	0.203028	114713	115077	743-754	1.000000	88.3
ndhG-ndhI_p3	ndhG-ndhI	3	TGGGAAGTAACACCACTCCT	GCTGCGAGATACATTGGTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.172305	114713	115077	898-909	1.000000	88.2
ndhG-ndhI_p4	ndhG-ndhI	4	CGCGTAGAGTTGAAGGGAAA	GCTGCGAGATACATTGGTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.134573	114713	115077	1425-1436	1.000000	87.5
ndhG-ndhI_p5	ndhG-ndhI	5	TCCCAACGAAAAGGCAGAAA	GCTGCGAGATACATTGGTCA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.120076	114713	115077	927-938	0.875000	80.1
ycf1_p1	ycf1	1	GCAAGAGCACAGGCAATTTT	TGCACCAATTTACGTGGACA	20	20	45.0	45.0	57.8	58.0	0.0	0.0	0.190623	123752	123871	471-660	0.875000	85.5
ycf1_p2	ycf1	2	CGGGTTTTGTGGGTTTTAGC	TGCACCAATTTACGTGGACA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.174303	123752	123871	720-909	0.750000	79.4
ycf1_p3	ycf1	3	GCGGGTTTTGTGGGTTTTAG	TGCACCAATTTACGTGGACA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.174303	123752	123871	721-910	0.750000	79.4
ycf1_p4	ycf1	4	CCCTTGTCTTTTCGTCCACA	TGCACCAATTTACGTGGACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.069502	123752	123871	477-513	0.250000	53.4
ycf1_p5	ycf1	5	CCCTTGTCTTTTCGTCCACA	TCCTTTTTCCCAGTTCGACG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.164509	123752	123871	705-741	0.250000	52.7
ndhF-ndhD_p1	ndhF-ndhD	1	AAGAAGAAGTCCCAGTCCGA	TTTGACCGCTGTACATTGCT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.120246	127823	128142	376-523	0.875000	85.9
ndhF-ndhD_p2	ndhF-ndhD	2	AAGAAGAAGTCCCAGTCCGA	GCCTACTACCACCAACCAAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.120547	127823	128142	852-999	0.875000	82.0
ndhF-ndhD_p3	ndhF-ndhD	3	AAGAAGAAGTCCCAGTCCGA	ACTACCACCAACCAAGGAGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.123401	127823	128142	848-995	0.875000	82.0
ndhF-ndhD_p4	ndhF-ndhD	4	AAGAAGAAGTCCCAGTCCGA	ATTCCACCCATTTCGTCGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035298	127823	128142	1100-1247	0.875000	81.8
ndhF-ndhD_p5	ndhF-ndhD	5	ACACTTTGAAAGGCCCACAT	ATTCCACCCATTTCGTCGAG	20	20	45.0	50.0	57.9	58.0	10.0	0.0	0.110786	127823	128142	1150-1297	0.875000	81.0
trnL-UAG-trnN-GUU_p1	trnL-UAG-trnN-GUU	1	TACCGATTTCACCATAGCGG	CTACCACTGAGCTACTGAGGA	20	21	50.0	52.4	57.5	58.0	0.0	11.2	1.525488	130670	131965	1172-1565	0.875000	74.8
trnL-UAG-trnN-GUU_p2	trnL-UAG-trnN-GUU	2	TACCGATTTCACCATAGCGG	TCTACCACTGAGCTACTGAGG	20	21	50.0	52.4	57.5	58.0	0.0	0.0	1.525488	130670	131965	1173-1566	0.875000	74.8
trnL-UAG-trnN-GUU_p3	trnL-UAG-trnN-GUU	3	TACCGATTTCACCATAGCGG	TACCACTGAGCTACTGAGGA	20	20	50.0	50.0	57.5	56.8	0.0	11.2	1.715561	130670	131965	1171-1564	0.875000	73.3
trnL-UAG-trnN-GUU_p4	trnL-UAG-trnN-GUU	4	TACCGATTTCACCATAGCGG	CGAACGCTCTACCACTGAG	20	19	50.0	57.9	57.5	57.7	0.0	0.0	1.833716	130670	131965	1180-1573	0.875000	72.3
trnL-UAG-trnN-GUU_p5	trnL-UAG-trnN-GUU	5	TACCGATTTCACCATAGCGG	CTACCACTGAGCTACTGAGG	20	20	50.0	55.0	57.5	56.5	0.0	0.0	2.052712	130670	131965	1172-1565	0.875000	70.6