primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpl2-rps19_p1	rpl2-rps19	1	ATGGGGTCATACTGCACTTG	GTCAGCTACAAAAGGGCCTT	20	20	50.0	50.0	57.6	58.1	0.0	14.5	0.514086	2081	2135	182-182	1.000000	76.3
rpl2-rps19_p2	rpl2-rps19	2	CATGGGGTCATACTGCACTT	GTCAGCTACAAAAGGGCCTT	20	20	50.0	50.0	57.6	58.1	0.0	14.5	0.514086	2081	2135	183-183	1.000000	76.3
rpl2-rps19_p3	rpl2-rps19	3	GGGTCATACTGCACTTGGAA	GTCAGCTACAAAAGGGCCTT	20	20	50.0	50.0	57.5	58.1	0.0	14.5	0.578881	2081	2135	179-179	1.000000	75.8
rpl2-rps19_p4	rpl2-rps19	4	ATGGGGTCATACTGCACTTG	ATGGTCAGCTACAAAAGGGC	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.585720	2081	2135	185-185	1.000000	75.7
rpl2-rps19_p5	rpl2-rps19	5	CATGGGGTCATACTGCACTT	ATGGTCAGCTACAAAAGGGC	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.585720	2081	2135	186-186	1.000000	75.7
petL-psbE_p1	petL-psbE	1	AGCTAAAAACAAAGAGGCAAACA	CAAAAGGGCGTTCTCCTGTA	23	20	34.8	50.0	57.7	57.8	0.0	0.0	3.516490	17587	18339	837-838	1.000000	52.3
petL-psbE_p2	petL-psbE	2	AGCTAAAAACAAAGAGGCAAACA	GGGCGTTCTCCTGTATTTCC	23	20	34.8	55.0	57.7	58.3	0.0	0.0	3.672705	17587	18339	832-833	1.000000	51.0
petL-psbE_p3	petL-psbE	3	AGCTAAAAACAAAGAGGCAAACA	GGCGTTCTCCTGTATTTCCA	23	20	34.8	50.0	57.7	57.6	0.0	0.0	3.734168	17587	18339	831-832	1.000000	50.5
petL-psbE_p4	petL-psbE	4	AGCTAAAAACAAAGAGGCAAACA	TATCAGCAAAAGGGCGTTCT	23	20	34.8	45.0	57.7	57.5	0.0	0.0	3.827517	17587	18339	843-844	1.000000	49.8
petL-psbE_p5	petL-psbE	5	GCTAAAAACAAAGAGGCAAACA	CAAAAGGGCGTTCTCCTGTA	22	20	36.4	50.0	56.3	57.8	0.0	0.0	3.906743	17587	18339	836-837	1.000000	49.1
psbJ-petA_p1	psbJ-petA	1	AGGTACGGTAGCTGGTACTC	TCAATTACGTGTTCAAGGTCT	20	21	55.0	38.1	58.0	55.2	18.3	0.0	3.862493	19111	20134	1227-1241	1.000000	49.5
psbJ-petA_p2	psbJ-petA	2	GGTACGGTAGCTGGTACTCT	TCAATTACGTGTTCAAGGTCT	20	21	55.0	38.1	58.0	55.2	15.7	0.0	3.862493	19111	20134	1226-1240	1.000000	49.5
psbJ-petA_p3	psbJ-petA	3	ACGGTAGCTGGTACTCTTGT	TCAATTACGTGTTCAAGGTCT	20	21	50.0	38.1	58.1	55.2	15.7	0.0	3.891994	19111	20134	1223-1237	1.000000	49.3
psbJ-petA_p4	psbJ-petA	4	AGGTACGGTAGCTGGTACTC	TGCATCTGTTATCTTAGCACA	20	21	55.0	38.1	58.0	55.0	18.3	0.0	4.016886	19111	20134	1194-1208	1.000000	48.3
psbJ-petA_p5	psbJ-petA	5	GGTACGGTAGCTGGTACTCT	TGCATCTGTTATCTTAGCACA	20	21	55.0	38.1	58.0	55.0	15.7	0.0	4.016886	19111	20134	1193-1207	1.000000	48.3
trnV-UAC_p1	trnV-UAC	1	CCATGCGCCACTTTTAACAG	GGATCCAAGCATCACCCATT	20	20	50.0	50.0	58.0	57.9	4.2	7.0	0.096434	29611	30235	1546-1548	1.000000	79.5
trnV-UAC_p2	trnV-UAC	2	CCATGCGCCACTTTTAACAG	CGTGAGCCAATTAATGACGC	20	20	50.0	50.0	58.0	57.9	4.2	0.0	0.109898	29611	30235	1959-1961	1.000000	79.3
trnV-UAC_p3	trnV-UAC	3	CCATGCGCCACTTTTAACAG	AACTGGATCCAAGCATCACC	20	20	50.0	50.0	58.0	57.9	4.2	17.0	0.153535	29611	30235	1550-1552	1.000000	79.0
trnV-UAC_p4	trnV-UAC	4	GAAAAGAGCAAAAGCACGCT	GGATCCAAGCATCACCCATT	20	20	45.0	50.0	57.9	57.9	0.0	7.0	0.203149	29611	30235	1297-1299	1.000000	78.7
trnV-UAC_p5	trnV-UAC	5	GCGCCACTTTTAACAGCTTT	GGATCCAAGCATCACCCATT	20	20	45.0	50.0	57.9	57.9	0.0	7.0	0.211889	29611	30235	1542-1544	1.000000	78.6
trnT-UGU-rps4_p1	trnT-UGU-rps4	1	GATGGTCATCGGTTCGACTC	GAGGTCCTCGATAACGGGA	20	19	55.0	57.9	58.2	57.9	0.0	2.3	1.265289	33095	33392	352-353	1.000000	71.2
trnT-UGU-rps4_p2	trnT-UGU-rps4	2	TGCTTAGCTCAGAGGTCAGA	GAGGTCCTCGATAACGGGA	20	19	50.0	57.9	57.8	57.9	14.0	2.3	1.328965	33095	33392	390-391	1.000000	70.6
trnT-UGU-rps4_p3	trnT-UGU-rps4	3	GCACTTGTAATGCGATGGTC	GAGGTCCTCGATAACGGGA	20	19	50.0	57.9	57.5	57.9	0.0	2.3	1.565330	33095	33392	365-366	1.000000	68.8
trnT-UGU-rps4_p4	trnT-UGU-rps4	4	AGAGCACCGCACTTGTAATG	GAGGTCCTCGATAACGGGA	20	19	50.0	57.9	58.6	57.9	0.0	2.3	1.663969	33095	33392	373-374	1.000000	68.0
trnT-UGU-rps4_p5	trnT-UGU-rps4	5	CAGAGCACCGCACTTGTAAT	GAGGTCCTCGATAACGGGA	20	19	50.0	57.9	58.6	57.9	0.0	2.3	1.663969	33095	33392	374-375	1.000000	68.0
trnfM-CAU-trnG-GCC_p1	trnfM-CAU-trnG-GCC	1	GTAGCTCGCAAGGCTCATAA	AATATGCGGGTTCGATTCCC	20	20	50.0	50.0	57.8	58.1	0.0	14.1	0.332859	41648	41760	196-196	1.000000	79.0
trnfM-CAU-trnG-GCC_p2	trnfM-CAU-trnG-GCC	2	TAGCTCGCAAGGCTCATAAC	AATATGCGGGTTCGATTCCC	20	20	50.0	50.0	57.8	58.1	0.0	14.1	0.332859	41648	41760	195-195	1.000000	79.0
trnfM-CAU-trnG-GCC_p3	trnfM-CAU-trnG-GCC	3	GTAGCTCGCAAGGCTCATAA	AAGGAGAATATGCGGGTTCG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.465562	41648	41760	202-202	1.000000	77.9
trnfM-CAU-trnG-GCC_p4	trnfM-CAU-trnG-GCC	4	TAGCTCGCAAGGCTCATAAC	AAGGAGAATATGCGGGTTCG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.465562	41648	41760	201-201	1.000000	77.9
trnfM-CAU-trnG-GCC_p5	trnfM-CAU-trnG-GCC	5	TAGCTCGCAAGGCTCATAAC	TTGCCAAGGAGAATATGCGG	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.545929	41648	41760	206-206	1.000000	77.2
trnG-GCC-psbZ_p1	trnG-GCC-psbZ	1	GGGAATCGAACCCGCATATT	CTTGCCTCTCCTGATGGTTG	20	20	50.0	55.0	58.1	58.3	13.9	0.0	0.360081	41832	42080	421-421	1.000000	77.5
trnG-GCC-psbZ_p2	trnG-GCC-psbZ	2	CGAACCCGCATATTCTCCTT	CTTGCCTCTCCTGATGGTTG	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.492784	41832	42080	415-415	1.000000	76.4
trnG-GCC-psbZ_p3	trnG-GCC-psbZ	3	CCGCATATTCTCCTTGGCAA	CTTGCCTCTCCTGATGGTTG	20	20	50.0	55.0	58.3	58.3	0.0	0.0	0.573152	41832	42080	410-410	1.000000	75.8
trnG-GCC-psbZ_p4	trnG-GCC-psbZ	4	AACCCGCATATTCTCCTTGG	CTTGCCTCTCCTGATGGTTG	20	20	50.0	55.0	57.7	58.3	0.0	0.0	0.600701	41832	42080	413-413	1.000000	75.6
trnG-GCC-psbZ_p5	trnG-GCC-psbZ	5	ATCGAACCCGCATATTCTCC	CTTGCCTCTCCTGATGGTTG	20	20	50.0	55.0	57.5	58.3	0.0	0.0	0.709657	41832	42080	417-417	1.000000	74.7
psbZ-trnS-UGA_p1	psbZ-trnS-UGA	1	CAACCATCAGGAGAGGCAAG	TTTTCAAGACCGGAGCCATC	20	20	55.0	50.0	58.3	58.2	0.0	9.5	0.431074	42270	42483	352-352	1.000000	76.8
psbZ-trnS-UGA_p2	psbZ-trnS-UGA	2	GCACGACAGGTACACCAATT	TTTTCAAGACCGGAGCCATC	20	20	50.0	50.0	58.5	58.2	0.4	9.5	0.659375	42270	42483	333-333	1.000000	75.0
psbZ-trnS-UGA_p3	psbZ-trnS-UGA	3	CAACCATCAGGAGAGGCAAG	GTTTTCAAGACCGGAGCCAT	20	20	55.0	50.0	58.3	58.5	0.0	9.5	0.717562	42270	42483	353-353	1.000000	74.5
psbZ-trnS-UGA_p4	psbZ-trnS-UGA	4	TGACCAACCATCAGGAGAGG	TTTTCAAGACCGGAGCCATC	20	20	55.0	50.0	58.7	58.2	0.0	9.5	0.899287	42270	42483	356-356	1.000000	73.0
psbZ-trnS-UGA_p5	psbZ-trnS-UGA	5	GCACGACAGGTACACCAATT	GTTTTCAAGACCGGAGCCAT	20	20	50.0	50.0	58.5	58.5	0.4	9.5	0.945863	42270	42483	334-334	1.000000	72.7
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	GGGTTCGAATCCCTCTCTCT	GATCGTGATTTCGAGCCTGT	20	20	55.0	50.0	58.2	58.1	17.2	0.0	0.289034	42572	42665	162-162	1.000000	80.7
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	GATGGCTCCGGTCTTGAAAA	GATCGTGATTTCGAGCCTGT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.250889	42572	42665	207-207	1.000000	80.3
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	GATGGCTCCGGTCTTGAAAA	TGATCGTGATTTCGAGCCTG	20	20	50.0	50.0	58.2	58.1	0.0	2.2	0.252222	42572	42665	208-208	1.000000	80.3
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	GATGGCTCCGGTCTTGAAAA	CGAGCCTGTTCTTTCCATGA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.277925	42572	42665	196-196	1.000000	80.2
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	GATGGCTCCGGTCTTGAAAA	TCGAGCCTGTTCTTTCCATG	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.277925	42572	42665	197-197	1.000000	80.2
ycf2_p1	ycf2	1	ACGAAAGCCCACTTTACTGA	CTCACCAATCTGGCAAAATGA	20	21	45.0	42.9	57.1	56.8	0.0	0.0	3.146020	49913	50032	783-783	1.000000	55.8
ycf2_p2	ycf2	2	ACGAAAGCCCACTTTACTGA	TCTCACCAATCTGGCAAAATG	20	21	45.0	42.9	57.1	56.8	0.0	0.0	3.146020	49913	50032	784-784	1.000000	55.8
ycf2_p3	ycf2	3	ACGAAAGCCCACTTTACTGA	TCTCACCAATCTGGCAAAATGA	20	22	45.0	40.9	57.1	58.2	0.0	0.0	3.157359	49913	50032	784-784	1.000000	55.7
ycf2_p4	ycf2	4	ACGAAAGCCCACTTTACTGA	TCACCAATCTGGCAAAATGA	20	20	45.0	40.0	57.1	55.5	0.0	0.0	3.402667	49913	50032	782-782	1.000000	53.7
ycf2_p5	ycf2	5	AACGAAAGCCCACTTTACTGA	CTCACCAATCTGGCAAAATGA	21	21	42.9	42.9	57.8	56.8	0.0	0.0	3.426109	49913	50032	784-784	1.000000	53.5
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	TAAAGGCGGTTTTCAGTGCT	CACGGTTTGGGAAGAGATGT	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.209344	55165	55409	431-433	1.000000	79.8
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	GTAAAGGCGGTTTTCAGTGC	CACGGTTTGGGAAGAGATGT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.231287	55165	55409	432-434	1.000000	79.6
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	AAGCAGCTTCCCAAATTGGA	CACGGTTTGGGAAGAGATGT	20	20	45.0	50.0	58.0	57.8	14.6	0.0	0.199014	55165	55409	646-648	1.000000	79.4
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	CATTCACGACCCATGTAGCA	CACGGTTTGGGAAGAGATGT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.207769	55165	55409	737-739	1.000000	79.2
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	CCGCAACAGGAGCTGAATAT	CACGGTTTGGGAAGAGATGT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.225962	55165	55409	802-804	1.000000	79.0
ycf12-trnG-UCC_p1	ycf12-trnG-UCC	1	ATTAGCTGCACGTAAAGGCA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	57.9	55.5	1.0	0.0	3.643792	61963	62096	196-196	1.000000	55.3
ycf12-trnG-UCC_p2	ycf12-trnG-UCC	2	TTAGCTGCACGTAAAGGCAA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.1	55.5	1.0	0.0	3.650282	61963	62096	195-195	1.000000	55.3
ycf12-trnG-UCC_p3	ycf12-trnG-UCC	3	ATTAGCTGCACGTAAAGGCA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	57.9	57.3	1.0	0.0	3.776508	61963	62096	199-199	1.000000	54.2
ycf12-trnG-UCC_p4	ycf12-trnG-UCC	4	ATTAGCTGCACGTAAAGGCA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	57.9	57.3	1.0	0.0	3.776508	61963	62096	198-198	1.000000	54.2
ycf12-trnG-UCC_p5	ycf12-trnG-UCC	5	TTAGCTGCACGTAAAGGCAA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.1	57.3	1.0	0.0	3.782998	61963	62096	197-197	1.000000	54.2
trnG-UCC_p1	trnG-UCC	1	ATTAGCTGCACGTAAAGGCA	AATTGGCAAGAGGTCAACGA	20	20	45.0	45.0	57.9	57.7	1.0	6.0	0.378302	62097	62747	1454-1457	1.000000	78.3
trnG-UCC_p2	trnG-UCC	2	TTAGCTGCACGTAAAGGCAA	AATTGGCAAGAGGTCAACGA	20	20	45.0	45.0	58.1	57.7	1.0	6.0	0.384792	62097	62747	1453-1456	1.000000	78.3
trnG-UCC_p3	trnG-UCC	3	ATTAGCTGCACGTAAAGGCA	GGCAAGAGGTCAACGACTAC	20	20	45.0	55.0	57.9	58.3	1.0	0.0	0.396744	62097	62747	1450-1453	1.000000	78.2
trnG-UCC_p4	trnG-UCC	4	TTAGCTGCACGTAAAGGCAA	GGCAAGAGGTCAACGACTAC	20	20	45.0	55.0	58.1	58.3	1.0	0.0	0.403234	62097	62747	1449-1452	1.000000	78.2
trnG-UCC_p5	trnG-UCC	5	ATTAGCTGCACGTAAAGGCA	CAATTGGCAAGAGGTCAACG	20	20	45.0	50.0	57.9	57.7	1.0	6.0	0.444504	62097	62747	1455-1458	1.000000	77.8
rpoC1-rpoB_p1	rpoC1-rpoB	1	GCCCAACTGCGTATTTGTTC	ACTGCTCCAGAATCTTTTCGA	20	21	50.0	42.9	58.0	57.3	3.1	0.0	1.707951	75666	75689	209-209	1.000000	67.1
rpoC1-rpoB_p2	rpoC1-rpoB	2	TTCAGCCCAACTGCGTATTT	ACTGCTCCAGAATCTTTTCGA	20	21	45.0	42.9	58.1	57.3	0.0	0.0	1.780852	75666	75689	213-213	1.000000	66.5
rpoC1-rpoB_p3	rpoC1-rpoB	3	TTTCAGCCCAACTGCGTATT	ACTGCTCCAGAATCTTTTCGA	20	21	45.0	42.9	58.1	57.3	0.0	0.0	1.780852	75666	75689	214-214	1.000000	66.5
rpoC1-rpoB_p4	rpoC1-rpoB	4	AGCCCAACTGCGTATTTGTT	ACTGCTCCAGAATCTTTTCGA	20	21	45.0	42.9	58.4	57.3	3.1	0.0	2.068981	75666	75689	210-210	1.000000	64.2
rpoC1-rpoB_p5	rpoC1-rpoB	5	CTTTCAGCCCAACTGCGTAT	ACTGCTCCAGAATCTTTTCGA	20	21	50.0	42.9	58.5	57.3	0.0	0.0	2.231984	75666	75689	215-215	1.000000	62.9
rpoB_p1	rpoB	1	GTCCGGCATTCATACCTTCA	AGACCTCTGCCTTACCACTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.357078	78288	78887	1593-1593	0.500000	57.1
rpoB_p2	rpoB	2	GTCCGGCATTCATACCTTCA	TTACCACTTGGCCATATCGC	20	20	50.0	50.0	57.7	58.0	0.0	6.1	0.363789	78288	78887	1582-1582	0.500000	57.1
rpoB_p3	rpoB	3	GTCCGGCATTCATACCTTCA	GACCTCTGCCTTACCACTTG	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.426188	78288	78887	1592-1592	0.500000	56.6
rpoB_p4	rpoB	4	TGAGTCCGGCATTCATACCT	AGACCTCTGCCTTACCACTT	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.535435	78288	78887	1596-1596	0.500000	55.7
rpoB_p5	rpoB	5	TGAGTCCGGCATTCATACCT	TTACCACTTGGCCATATCGC	20	20	50.0	50.0	58.5	58.0	0.0	6.1	0.542147	78288	78887	1585-1585	0.500000	55.7