primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK(TTT)-trnR(TCT)_p1	trnK(TTT)-trnR(TCT)	1	TAGTTCTGGGTTCGAGTCCC	TTCGATTCCTACTGGGCCTA	20	20	55.0	50.0	58.4	57.5	17.5	4.5	0.911089	12651	12719	105-119	1.000000	84.7
trnK(TTT)-trnR(TCT)_p2	trnK(TTT)-trnR(TCT)	2	ACCGAATAGTTCTGGGTTCG	TTCGATTCCTACTGGGCCTA	20	20	50.0	50.0	57.3	57.5	0.0	4.5	1.132269	12651	12719	111-125	1.000000	82.9
trnK(TTT)-trnR(TCT)_p3	trnK(TTT)-trnR(TCT)	3	TAGTTCTGGGTTCGAGTCCC	GGGCCTATCGTCTAACGGAT	20	20	55.0	55.0	58.4	58.7	17.5	0.8	1.191340	12651	12719	158-172	1.000000	82.5
trnK(TTT)-trnR(TCT)_p4	trnK(TTT)-trnR(TCT)	4	ACCGAATAGTTCTGGGTTCG	GGGCCTATCGTCTAACGGAT	20	20	50.0	55.0	57.3	58.7	0.0	0.8	1.412520	12651	12719	164-178	1.000000	80.7
trnK(TTT)-trnR(TCT)_p5	trnK(TTT)-trnR(TCT)	5	CCGAATAGTTCTGGGTTCGA	TTCGATTCCTACTGGGCCTA	20	20	50.0	50.0	57.0	57.5	7.8	4.5	1.418262	12651	12719	110-124	1.000000	80.7
trnV(TAC)-trnY(GTA)_p1	trnV(TAC)-trnY(GTA)	1	TACCGCTGAGTTAATTGCCC	GATTTACAATCCGCCCCCTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.100046	12871	13050	234-241	1.000000	89.9
trnV(TAC)-trnY(GTA)_p2	trnV(TAC)-trnY(GTA)	2	AGACACTCTACCGCTGAGTT	GATTTACAATCCGCCCCCTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.154949	12871	13050	242-249	1.000000	89.4
trnV(TAC)-trnY(GTA)_p3	trnV(TAC)-trnY(GTA)	3	CTGTGGCCTTCTGCTTGTAA	GATTTACAATCCGCCCCCTT	20	20	50.0	50.0	58.1	57.9	3.3	0.0	0.171146	12871	13050	265-272	1.000000	89.0
trnV(TAC)-trnY(GTA)_p4	trnV(TAC)-trnY(GTA)	4	TGTGGCCTTCTGCTTGTAAG	GATTTACAATCCGCCCCCTT	20	20	50.0	50.0	58.1	57.9	3.3	0.0	0.171146	12871	13050	264-271	1.000000	89.0
trnV(TAC)-trnY(GTA)_p5	trnV(TAC)-trnY(GTA)	5	TACCGCTGAGTTAATTGCCC	CGTAACTTGACGTAGCGGAT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.217065	12871	13050	251-258	1.000000	88.8
trnM(CAT)-trnS(GCT)_p1	trnM(CAT)-trnS(GCT)	1	AGTGGTTAGAGCAGGGGATT	CTTTCGACCACTCAGCCATT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.213899	17102	17191	161-175	1.000000	90.3
trnM(CAT)-trnS(GCT)_p2	trnM(CAT)-trnS(GCT)	2	AGTGGTTAGAGCAGGGGATT	TTCGACCACTCAGCCATTTC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.219002	17102	17191	159-173	1.000000	90.2
trnM(CAT)-trnS(GCT)_p3	trnM(CAT)-trnS(GCT)	3	TGGTTAGAGCAGGGGATTCA	CTTTCGACCACTCAGCCATT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.221072	17102	17191	159-173	1.000000	90.2
trnM(CAT)-trnS(GCT)_p4	trnM(CAT)-trnS(GCT)	4	TGGTTAGAGCAGGGGATTCA	TTCGACCACTCAGCCATTTC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.226175	17102	17191	157-171	1.000000	90.2
trnM(CAT)-trnS(GCT)_p5	trnM(CAT)-trnS(GCT)	5	AGTGGTTAGAGCAGGGGATT	GACCACTCAGCCATTTCTCC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.283416	17102	17191	156-170	1.000000	89.7
petA_p1	petA	1	AGTACACCAAGAAGACGAACT	AAGCATCCATCTTTTAAAAAGT	21	22	42.9	27.3	56.6	52.5	0.0	1.7	9.867178	30440	31384	1052-1052	1.000000	44.0
petA_p2	petA	2	AGTACACCAAGAAGACGAACT	AAAGCATCCATCTTTTAAAAAGT	21	23	42.9	26.1	56.6	53.4	0.0	1.7	10.001558	30440	31384	1053-1053	1.000000	44.0
petA_p3	petA	3	AGTACACCAAGAAGACGAACT	AGCATCCATCTTTTAAAAAGTATTTCC	21	27	42.9	29.6	56.6	57.1	0.0	1.7	10.252111	30440	31384	1051-1051	1.000000	44.0
petA_p4	petA	4	AGTACACCAAGAAGACGAACT	GCATCCATCTTTTAAAAAGTATTTCCT	21	27	42.9	29.6	56.6	57.1	0.0	1.7	10.252111	30440	31384	1050-1050	1.000000	44.0
petA_p5	petA	5	AGTACACCAAGAAGACGAACT	ACTAAAGCATCCATCTTTTAAAAAGT	21	26	42.9	26.9	56.6	56.1	0.0	1.7	10.256036	30440	31384	1056-1056	1.000000	43.9
rpoB_p1	rpoB	1	ACCATGTACGCTAGCAGTTG	ACCAAATCGAATCCAAGATCCA	20	22	50.0	40.9	58.0	57.8	16.4	0.0	2.245224	36875	36994	1153-1153	1.000000	69.3
rpoB_p2	rpoB	2	CACCATGTACGCTAGCAGTT	ACCAAATCGAATCCAAGATCCA	20	22	50.0	40.9	58.0	57.8	16.4	0.0	2.245224	36875	36994	1154-1154	1.000000	69.2
rpoB_p3	rpoB	3	TGCAGGACAAGCTTCCATTT	ACCAAATCGAATCCAAGATCCA	20	22	45.0	40.9	58.0	57.8	7.2	0.0	2.235430	36875	36994	850-850	0.500000	42.1
rpoB_p4	rpoB	4	TTACCTGCAGGACAAGCTTC	ACCAAATCGAATCCAAGATCCA	20	22	50.0	40.9	57.8	57.8	39.2	0.0	2.420083	36875	36994	855-855	0.500000	40.6
rpoB_p5	rpoB	5	AAACCACCATGTACGCTAGC	ACCAAATCGAATCCAAGATCCA	20	22	50.0	40.9	58.3	57.8	4.1	0.0	2.500916	36875	36994	1158-1158	0.500000	40.0
rpoC2_p1	rpoC2	1	TGCAACTAAAACAGGAGGGAC	TGCGTTTCTTCTTCCAACCA	21	20	47.6	45.0	58.1	58.0	0.0	0.0	1.186817	44174	44293	258-258	0.500000	50.5
rpoC2_p2	rpoC2	2	CCAGATTCTTTTTCCCAGCCT	TGCGTTTCTTCTTCCAACCA	21	20	47.6	45.0	58.2	58.0	0.0	0.0	1.232903	44174	44293	848-848	0.500000	50.1
rpoC2_p3	rpoC2	3	TCCAGATTCTTTTTCCCAGCC	TGCGTTTCTTCTTCCAACCA	21	20	47.6	45.0	58.2	58.0	0.0	0.0	1.237885	44174	44293	849-849	0.500000	50.1
rpoC2_p4	rpoC2	4	CCAGATTCTTTTTCCCAGCC	TGCGTTTCTTCTTCCAACCA	20	20	50.0	45.0	56.7	58.0	0.0	0.0	1.380545	44174	44293	848-848	0.500000	49.0
rpoC2_p5	rpoC2	5	TGCAACTAAAACAGGAGGGA	TGCGTTTCTTCTTCCAACCA	20	20	45.0	45.0	56.7	58.0	0.0	0.0	1.382291	44174	44293	258-258	0.500000	48.9
ftsH_p1	ftsH	1	TCCTGAAGGTGGTCGTTACT	CCATTTTGATAGCGAGTGGC	20	20	50.0	50.0	58.0	57.2	0.0	0.0	0.768619	56821	58692	2968-2968	0.500000	53.9
ftsH_p2	ftsH	2	TCCTGAAGGTGGTCGTTACT	CGCAAGTACTTGTGCAGACA	20	20	50.0	50.0	58.0	58.8	0.0	30.3	0.788369	56821	58692	2906-2906	0.500000	53.7
ftsH_p3	ftsH	3	AACGCGTTTGATGCTAGCTA	CGAACAACAGCAGGATAACG	20	20	45.0	50.0	58.0	57.2	20.8	0.0	0.792305	56821	58692	2650-2650	0.500000	53.7
ftsH_p4	ftsH	4	TAACGCGTTTGATGCTAGCT	CGAACAACAGCAGGATAACG	20	20	45.0	50.0	58.0	57.2	31.5	0.0	0.792305	56821	58692	2651-2651	0.500000	53.7
ftsH_p5	ftsH	5	GGGCAATGAACGTTGAATGG	CGAACAACAGCAGGATAACG	20	20	50.0	50.0	58.0	57.2	5.0	0.0	0.792807	56821	58692	2805-2805	0.500000	53.7
ycf45_p1	ycf45	1	ACGTCGACATGCGTATAAGG	GATTTGAACCTACGACCCCC	20	20	50.0	55.0	57.9	58.0	17.2	0.0	0.149452	60402	61748	2210-2210	0.500000	58.8
ycf45_p2	ycf45	2	ACGTCGACATGCGTATAAGG	GGATTTGAACCTACGACCCC	20	20	50.0	55.0	57.9	58.0	17.2	0.0	0.149452	60402	61748	2211-2211	0.500000	58.8
ycf45_p3	ycf45	3	AAAAACAAGGAAAGCCGCAG	GATTTGAACCTACGACCCCC	20	20	45.0	55.0	57.8	58.0	0.0	0.0	0.257641	60402	61748	2267-2267	0.500000	57.9
ycf45_p4	ycf45	4	AAAAACAAGGAAAGCCGCAG	GGATTTGAACCTACGACCCC	20	20	45.0	55.0	57.8	58.0	0.0	0.0	0.257641	60402	61748	2268-2268	0.500000	57.9
ycf45_p5	ycf45	5	ACGTCGACATGCGTATAAGG	GGTTTTGGGGAATCACCGAT	20	20	50.0	50.0	57.9	58.2	17.2	0.0	0.268020	60402	61748	2573-2573	0.500000	57.9
ycf45-trnP(TGG)_p1	ycf45-trnP(TGG)	1	TGTAACGCTGAGTGATGACG	GATTTGAACCTACGACCCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.056916	61749	61978	878-878	0.500000	59.5
ycf45-trnP(TGG)_p2	ycf45-trnP(TGG)	2	TGTAACGCTGAGTGATGACG	GGATTTGAACCTACGACCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.056916	61749	61978	879-879	0.500000	59.5
ycf45-trnP(TGG)_p3	ycf45-trnP(TGG)	3	TGTAACGCTGAGTGATGACG	AAACGACTTCTCGGCAAACT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.073346	61749	61978	1414-1414	0.500000	59.4
ycf45-trnP(TGG)_p4	ycf45-trnP(TGG)	4	TGTAACGCTGAGTGATGACG	AACGACTTCTCGGCAAACTT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.073346	61749	61978	1413-1413	0.500000	59.4
ycf45-trnP(TGG)_p5	ycf45-trnP(TGG)	5	TGTAACGCTGAGTGATGACG	GACTTCTCGGCAAACTTCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.143859	61749	61978	1410-1410	0.500000	58.8
rns-trnI(GAT)_p1	rns-trnI(GAT)	1	GACTGGGGTGAAGTCGTAAC	TACCAGCTGAGCTATACCCC	20	20	55.0	55.0	57.9	58.0	0.0	17.4	0.087101	64881	64936	208-209	1.000000	80.8
rns-trnI(GAT)_p2	rns-trnI(GAT)	2	GACTGGGGTGAAGTCGTAAC	ATTTGAACCAGAGACCTCGC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.176584	64881	64936	172-173	1.000000	80.4
rns-trnI(GAT)_p3	rns-trnI(GAT)	3	GACTGGGGTGAAGTCGTAAC	CCTTGGGCCATTCTGGATTT	20	20	55.0	50.0	57.9	58.1	0.0	7.3	0.205915	64881	64936	188-189	1.000000	80.1
rns-trnI(GAT)_p4	rns-trnI(GAT)	4	GGGGTGAAGTCGTAACAAGG	TACCAGCTGAGCTATACCCC	20	20	55.0	55.0	58.2	58.0	0.0	17.4	0.206521	64881	64936	204-205	1.000000	79.9
rns-trnI(GAT)_p5	rns-trnI(GAT)	5	TGCCCGAAGTCGTTACTCTA	TACCAGCTGAGCTATACCCC	20	20	50.0	55.0	58.2	58.0	0.0	17.4	0.186032	64881	64936	263-264	1.000000	79.7
rpl14-rps17_p1	rpl14-rps17	1	GCACCAGTATTATCTGCTACTGT	AGTTCAAGAATGTCGTCCTT	23	20	43.5	40.0	58.0	54.6	7.5	0.0	6.434869	85433	85442	139-139	1.000000	40.6
rpl14-rps17_p2	rpl14-rps17	2	AGCACCAGTATTATCTGCTACTG	AGTTCAAGAATGTCGTCCTT	23	20	43.5	40.0	57.8	54.6	2.4	0.0	6.592030	85433	85442	140-140	1.000000	40.6
rpl14-rps17_p3	rpl14-rps17	3	GCACCAGTATTATCTGCTACTGT	AGTTCAAGAATGTCGTCCTTT	23	21	43.5	38.1	58.0	55.5	7.5	0.0	6.597489	85433	85442	139-139	1.000000	40.6
rpl14-rps17_p4	rpl14-rps17	4	AGCACCAGTATTATCTGCTACTG	AGTTCAAGAATGTCGTCCTTT	23	21	43.5	38.1	57.8	55.5	2.4	0.0	6.754649	85433	85442	140-140	1.000000	40.6
rpl14-rps17_p5	rpl14-rps17	5	ACCACCTAGAACCCTAATACACA	AGTTCAAGAATGTCGTCCTT	23	20	43.5	40.0	58.3	54.6	0.0	0.0	6.693681	85433	85442	173-173	1.000000	40.5
syfB_p1	syfB	1	CCTGCCACCCCAGTTAAAAT	TCACAACCCACTGCCTTAAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087149	96104	98224	2959-2973	1.000000	87.7
syfB_p2	syfB	2	CTCGTAGGTTCGAATCCTGC	TCACAACCCACTGCCTTAAC	20	20	55.0	50.0	58.2	58.0	22.0	0.0	0.182891	96104	98224	2974-2988	1.000000	87.0
syfB_p3	syfB	3	CCACTCGTAGGTTCGAATCC	TCACAACCCACTGCCTTAAC	20	20	55.0	50.0	57.8	58.0	4.4	0.0	0.229647	96104	98224	2977-2991	1.000000	86.6
syfB_p4	syfB	4	ACAGGTACAGCAACATTCCG	TCACAACCCACTGCCTTAAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.215930	96104	98224	2769-2778	1.000000	86.5
syfB_p5	syfB	5	CCTGCCACCCCAGTTAAAAT	GCCTTAACCACTTGGCTACA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.257010	96104	98224	2947-2961	1.000000	86.4
ycf35_p1	ycf35	1	AGAGATGGTCGCCCTATTGA	TGGGCATTTGGTTAACAGCT	20	20	50.0	45.0	57.9	58.0	0.0	8.1	0.096347	99790	100158	1240-1249	1.000000	88.1
ycf35_p2	ycf35	2	AGAGATGGTCGCCCTATTGA	ATGGGCTGACATCATCAACC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.151075	99790	100158	1134-1143	1.000000	87.8
ycf35_p3	ycf35	3	AGAGATGGTCGCCCTATTGA	TTCTCAGCTATGCACGGTTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.098249	99790	100158	1451-1460	1.000000	87.5
ycf35_p4	ycf35	4	AGAGATGGTCGCCCTATTGA	CTCAGCTATGCACGGTTCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.103840	99790	100158	1449-1458	1.000000	87.5
ycf35_p5	ycf35	5	AGAGATGGTCGCCCTATTGA	AAGTCCCTCCGTTCCCAATA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.126971	99790	100158	794-794	0.500000	59.0
ccs1_p1	ccs1	1	ATTCCCATTCACGACCCATG	AGGCCGTTTTGATGAAGTCT	20	20	50.0	45.0	57.9	57.4	0.0	0.0	0.625760	101622	102887	2116-2116	1.000000	81.3
ccs1_p2	ccs1	2	ATGTTGTGCTCTGCTTGGAA	AGGCCGTTTTGATGAAGTCT	20	20	45.0	45.0	58.0	57.4	0.0	0.0	0.583150	101622	102887	2294-2294	1.000000	81.2
ccs1_p3	ccs1	3	TGTTGTGCTCTGCTTGGAAT	AGGCCGTTTTGATGAAGTCT	20	20	45.0	45.0	58.0	57.4	0.0	0.0	0.583150	101622	102887	2293-2293	1.000000	81.2
ccs1_p4	ccs1	4	GAACCGTGCATAGCTGAGAA	AGGCCGTTTTGATGAAGTCT	20	20	50.0	45.0	58.0	57.4	0.0	0.0	0.578768	101622	102887	2369-2369	1.000000	81.0
ccs1_p5	ccs1	5	AAGAACCGTGCATAGCTGAG	AGGCCGTTTTGATGAAGTCT	20	20	50.0	45.0	58.0	57.4	0.0	0.0	0.584359	101622	102887	2371-2371	1.000000	81.0
trnI(GAT)-rns_p1	trnI(GAT)-rns	1	TACCAGCTGAGCTATACCCC	GACTGGGGTGAAGTCGTAAC	20	20	55.0	55.0	58.0	57.9	17.4	0.0	0.087101	113039	113094	208-209	1.000000	80.8
trnI(GAT)-rns_p2	trnI(GAT)-rns	2	ATTTGAACCAGAGACCTCGC	GACTGGGGTGAAGTCGTAAC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.176584	113039	113094	172-173	1.000000	80.4
trnI(GAT)-rns_p3	trnI(GAT)-rns	3	CCTTGGGCCATTCTGGATTT	GACTGGGGTGAAGTCGTAAC	20	20	50.0	55.0	58.1	57.9	7.3	0.0	0.205915	113039	113094	188-189	1.000000	80.1
trnI(GAT)-rns_p4	trnI(GAT)-rns	4	TACCAGCTGAGCTATACCCC	GGGGTGAAGTCGTAACAAGG	20	20	55.0	55.0	58.0	58.2	17.4	0.0	0.206521	113039	113094	204-205	1.000000	79.9
trnI(GAT)-rns_p5	trnI(GAT)-rns	5	TACCAGCTGAGCTATACCCC	TGCCCGAAGTCGTTACTCTA	20	20	55.0	50.0	58.0	58.2	17.4	0.0	0.186032	113039	113094	263-264	1.000000	79.7