primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	AATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.364744	75	315	314-390	1.000000	89.1
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	AATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.7	57.9	0.0	13.4	0.397962	75	315	264-340	1.000000	88.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.406792	75	315	267-343	1.000000	88.7
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	TTGCCTTAATCCACTTGGCT	GCGCTAACCTTGGTATGGAA	20	20	45.0	50.0	57.4	58.0	0.0	0.0	0.635755	75	315	321-397	1.000000	86.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	TTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	20	20	45.0	55.0	57.4	57.9	0.0	13.4	0.668973	75	315	271-347	1.000000	86.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6532	7749	1078-1291	1.000000	90.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	6532	7749	1108-1321	1.000000	89.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6532	7749	1092-1305	1.000000	88.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GGATCGTGTCCTTCAAGTCG	TAAGGCATCGGGTTTTGGTC	20	20	55.0	50.0	58.4	58.2	2.9	0.0	0.540873	6532	7749	1122-1335	1.000000	87.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	GTAAGGCATCGGGTTTTGGT	20	20	45.0	50.0	58.1	58.5	8.1	0.0	0.597945	6532	7749	1109-1322	1.000000	87.2
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	ATGATCCGGGTCGTAATCCT	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.0	58.0	8.0	0.0	0.015705	8890	9033	245-279	1.000000	89.5
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	ATGATCCGGGTCGTAATCCT	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.0	58.0	8.0	0.0	0.017093	8890	9033	246-280	1.000000	89.4
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	ATGATCCGGGTCGTAATCCT	GACTAAAGCGTCGGATTGCT	20	20	50.0	50.0	58.0	58.4	8.0	2.3	0.369545	8890	9033	243-277	1.000000	86.7
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	ATGATCCGGGTCGTAATCCT	CTGAGTGGACTAAAGCGTCG	20	20	50.0	55.0	58.0	58.4	8.0	0.0	0.392262	8890	9033	250-284	1.000000	86.3
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	ATGATCCGGGTCGTAATCCT	ATGGCTGAGTGGACTAAAGC	20	20	50.0	50.0	58.0	57.6	8.0	5.7	0.421071	8890	9033	254-288	1.000000	85.9
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	TAATCAAACCGAGGGATCCC	20	20	50.0	50.0	58.0	56.7	0.0	24.6	1.320552	9122	10061	905-1220	1.000000	81.4
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	TAATCAAACCGAGGGATCCC	20	20	50.0	50.0	58.0	56.7	0.0	24.6	1.321940	9122	10061	904-1219	1.000000	81.4
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ACGGAAAGAGAGGGATTCGA	TAATCAAACCGAGGGATCCC	20	20	50.0	50.0	58.2	56.7	0.0	24.6	1.467317	9122	10061	959-1274	1.000000	80.3
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	CAATCCGACGCTTTAGTCCA	TTAATCAAACCGAGGGATCCC	20	21	50.0	47.6	58.0	57.4	0.0	24.6	1.575494	9122	10061	906-1221	1.000000	79.4
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	AATCCGACGCTTTAGTCCAC	TTAATCAAACCGAGGGATCCC	20	21	50.0	47.6	58.0	57.4	0.0	24.6	1.576882	9122	10061	905-1220	1.000000	79.4
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	ATGGAAATGAAAGGCGTCCA	TCAGGAACAGATGGAACGTT	20	20	45.0	45.0	57.8	56.8	0.0	0.0	1.435965	11240	11582	433-483	1.000000	77.9
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	ATGGAAATGAAAGGCGTCCA	TTCAGGAACAGATGGAACGT	20	20	45.0	45.0	57.8	56.8	0.0	0.0	1.435965	11240	11582	434-484	1.000000	77.9
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TGGAAATGAAAGGCGTCCAT	TCAGGAACAGATGGAACGTT	20	20	45.0	45.0	57.8	56.8	0.0	0.0	1.435965	11240	11582	432-482	1.000000	77.9
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	TGGAAATGAAAGGCGTCCAT	TTCAGGAACAGATGGAACGT	20	20	45.0	45.0	57.8	56.8	0.0	0.0	1.435965	11240	11582	433-483	1.000000	77.9
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TGGAAATGAAAGGCGTCCAT	TCAGGAACAGATGGAACGTTT	20	21	45.0	42.9	57.8	57.5	0.0	0.5	1.699839	11240	11582	432-482	1.000000	75.8
rps2-rpoC2_p1	rps2-rpoC2	1	GGTGCCATTCTAGGATTCCA	ACATCAGAACAATCATTTCTGGG	20	23	50.0	39.1	57.0	57.1	5.3	11.2	4.939258	17582	17796	349-370	0.956522	47.9
rps2-rpoC2_p2	rps2-rpoC2	2	ACTCCTGCTTCCATCATCTCT	ACATCAGAACAATCATTTCTGGG	21	23	47.6	39.1	58.2	57.1	0.0	11.2	5.147812	17582	17796	304-325	0.956522	47.9
rps2-rpoC2_p3	rps2-rpoC2	3	TGAACTCCTGCTTCCATCATC	ACATCAGAACAATCATTTCTGGG	21	23	47.6	39.1	57.7	57.1	0.0	11.2	5.182195	17582	17796	307-328	0.956522	47.8
rps2-rpoC2_p4	rps2-rpoC2	4	TGACCAAAATGAACTCCTGCT	ACATCAGAACAATCATTTCTGGG	21	23	42.9	39.1	57.8	57.1	0.0	11.2	5.137809	17582	17796	316-337	0.956522	47.7
rps2-rpoC2_p5	rps2-rpoC2	5	GGTGCCATTCTAGGATTCCA	CATCAGAACAATCATTTCTGGGA	20	23	50.0	39.1	57.0	56.8	5.3	11.2	5.192858	17582	17796	348-369	0.956522	47.4
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	33768	34974	1269-1429	1.000000	89.4
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	33768	34974	1272-1432	1.000000	89.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33768	34974	1259-1419	1.000000	88.4
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33768	34974	1262-1422	1.000000	88.2
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.395920	33768	34974	1246-1406	1.000000	87.8
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	20	20	55.0	50.0	58.0	56.8	10.3	3.3	1.224095	51519	52241	846-907	1.000000	76.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	20	20	55.0	50.0	58.0	56.8	10.3	3.3	1.226313	51519	52241	845-906	1.000000	76.6
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	51519	52241	810-871	1.000000	76.3
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	51519	52241	809-870	1.000000	76.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGATGGCC	20	21	55.0	47.6	58.0	57.7	10.3	0.0	1.299640	51519	52241	853-914	1.000000	76.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCCAGAAAATATCATATTCGT	GTTCGAGTCCGTATAGCCCT	23	20	34.8	55.0	56.1	58.7	0.0	0.0	5.564602	53928	54647	760-848	1.000000	49.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCCAGAAAATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	23	20	34.8	50.0	56.1	57.8	0.0	0.0	5.078536	53928	54647	772-860	1.000000	49.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGCCCAGAAAATATCATATTCGT	GAAGGTCTACGGTTCGAGTC	23	20	34.8	55.0	56.1	57.5	0.0	0.0	5.422308	53928	54647	771-859	1.000000	49.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGCCAAAATAGGAATAACACTTGA	AGAAGGTCTACGGTTCGAGT	24	20	33.3	50.0	56.8	57.8	0.0	0.0	5.352136	53928	54647	808-896	1.000000	49.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCAAAATAGGAATAACACTTGAG	AGAAGGTCTACGGTTCGAGT	25	20	36.0	50.0	57.8	57.8	0.0	0.0	5.354159	53928	54647	808-896	1.000000	49.5
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	24.9	12.5	1.868337	67774	69163	1409-1466	1.000000	74.3
psbE-petL_p2	psbE-petL	2	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	20	20	55.0	45.0	58.2	56.3	0.9	12.5	1.909925	67774	69163	1404-1461	1.000000	74.0
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	20	20	45.0	45.0	58.2	56.3	0.0	12.5	1.883014	67774	69163	1495-1552	1.000000	73.9
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	58.2	56.3	0.0	12.5	1.895919	67774	69163	1500-1557	1.000000	73.8
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	0.0	12.5	1.899345	67774	69163	1494-1551	1.000000	73.8
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144618	69736	69904	226-254	1.000000	88.3
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	69736	69904	224-252	1.000000	88.3
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	69736	69904	227-255	1.000000	88.3
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	69736	69904	237-265	1.000000	88.1
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137926	69736	69904	238-266	1.000000	88.1
ndhF-rpl32_p1	ndhF-rpl32	1	CCCCACGCGATTAGTTACAA	TGCTGCCCAATATCCTTTCC	20	20	50.0	50.0	58.0	57.9	15.9	0.0	0.084714	116289	117414	1615-1795	1.000000	88.7
ndhF-rpl32_p2	ndhF-rpl32	2	TTTTTGACAAGCATTCGCCG	TGCTGCCCAATATCCTTTCC	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.306927	116289	117414	1591-1771	1.000000	87.0
ndhF-rpl32_p3	ndhF-rpl32	3	GATTTAGCAACTGCACCAGC	TGCTGCCCAATATCCTTTCC	20	20	50.0	50.0	57.7	57.9	4.2	0.0	0.340508	116289	117414	1782-1962	1.000000	86.5
ndhF-rpl32_p4	ndhF-rpl32	4	CCCCACGCGATTAGTTACAA	GAAATCGTGTGCTTGCGTTT	20	20	50.0	45.0	58.0	58.2	15.9	0.0	0.258981	116289	117414	2036-2228	0.782609	79.2
ndhF-rpl32_p5	ndhF-rpl32	5	CCCCACGCGATTAGTTACAA	AAAAGCTTTCAATGCTGCCC	20	20	50.0	45.0	58.0	57.8	15.9	13.1	0.187300	116289	117414	1627-1777	0.347826	60.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGGAAATCTCTTTCTACGAAGA	AGCGTGTCTACCGATTTCAC	23	20	39.1	50.0	56.7	58.0	0.0	0.0	4.268717	117574	118672	1169-1421	1.000000	57.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGAAATCTCTTTCTACGAAGA	CAGCGTGTCTACCGATTTCA	23	20	39.1	50.0	56.7	58.0	0.0	0.0	4.270087	117574	118672	1170-1422	1.000000	57.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGAAATCTCTTTCTACGAAGA	TCGAACCGAGATGCTTTAGC	23	20	39.1	50.0	56.7	58.1	0.0	0.0	4.338316	117574	118672	1205-1457	1.000000	57.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGAAATCTCTTTCTACGAAGA	TCCTAAGAGCAGCGTGTCTA	23	20	39.1	50.0	56.7	57.9	0.0	0.0	4.385164	117574	118672	1179-1431	1.000000	56.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCATTGAAAGCTTTTTCATTAGGG	AGCGTGTCTACCGATTTCAC	24	20	37.5	50.0	57.6	58.0	14.0	0.0	4.420848	117574	118672	1189-1441	0.913043	51.3
ndhA_p1	ndhA	1	TGACCATAATTCATGAACCCAGT	GCCCGCCAGGTTTTATCAAT	23	20	39.1	50.0	57.8	58.9	11.1	0.0	4.080450	124200	126754	2769-2932	1.000000	55.4
ndhA_p2	ndhA	2	TGACCATAATTCATGAACCCAGT	GCCCGCCAGGTTTTATCAA	23	19	39.1	52.6	57.8	58.4	11.1	0.0	4.617807	124200	126754	2769-2932	1.000000	51.1
ndhA_p3	ndhA	3	TGACCATAATTCATGAACCCAGT	TGGGAGAAGTTGATCGTTGA	23	20	39.1	45.0	57.8	56.5	11.1	0.0	4.704332	124200	126754	2670-2833	1.000000	50.5
ndhA_p4	ndhA	4	TGACCATAATTCATGAACCCAG	GCCCGCCAGGTTTTATCAAT	22	20	40.9	50.0	56.1	58.9	11.1	0.0	4.768194	124200	126754	2769-2932	1.000000	49.9
ndhA_p5	ndhA	5	GACCATAATTCATGAACCCAGT	GCCCGCCAGGTTTTATCAAT	22	20	40.9	50.0	56.1	58.9	11.1	0.0	4.770616	124200	126754	2768-2931	1.000000	49.9
rpl2_p1	rpl2	1	CGCTTCAACCGGGTTATTCT	TTTTAGTGAACGTGGCACAA	20	20	50.0	40.0	58.3	56.1	13.0	19.1	2.146558	157543	159052	1676-1698	1.000000	63.3
rpl2_p2	rpl2	2	CGCTTCAACCGGGTTATTCT	TTTTTAGTGAACGTGGCACA	20	20	50.0	40.0	58.3	56.1	13.0	19.1	2.146558	157543	159052	1677-1699	1.000000	63.3
rpl2_p3	rpl2	3	ATCATTACGCTTCAACCGGG	TTTTAGTGAACGTGGCACAA	20	20	50.0	40.0	58.3	56.1	6.3	19.1	2.217026	157543	159052	1683-1705	1.000000	62.7
rpl2_p4	rpl2	4	ATCATTACGCTTCAACCGGG	TTTTTAGTGAACGTGGCACA	20	20	50.0	40.0	58.3	56.1	6.3	19.1	2.217026	157543	159052	1684-1706	1.000000	62.7
rpl2_p5	rpl2	5	CGCTTCAACCGGGTTATTCT	AGTGAACGTGGCACAATAAA	20	20	50.0	40.0	58.3	55.9	13.0	19.1	2.376746	157543	159052	1672-1694	1.000000	61.4