primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TCCGCCTAAATGGAAATGCA	ACGTAGTTGTACAGTGCAAT	20	20	45.0	40.0	57.9	55.0	0.0	7.4	3.098575	1388	4048	2822-2822	1.000000	55.2
trnK-UUU_p2	trnK-UUU	2	TCCGCCTAAATGGAAATGCA	CGTAGTTGTACAGTGCAATTCA	20	22	45.0	40.9	57.9	57.0	0.0	7.4	3.119514	1388	4048	2821-2821	1.000000	55.1
trnK-UUU_p3	trnK-UUU	3	TGAAGCGAGCTAAAACGGAA	ACGTAGTTGTACAGTGCAAT	20	20	45.0	40.0	57.8	55.0	0.0	7.4	3.120111	1388	4048	2785-2785	1.000000	55.1
trnK-UUU_p4	trnK-UUU	4	TTGAAGCGAGCTAAAACGGA	ACGTAGTTGTACAGTGCAAT	20	20	45.0	40.0	57.8	55.0	0.0	7.4	3.120111	1388	4048	2786-2786	1.000000	55.1
trnK-UUU_p5	trnK-UUU	5	TCCGCCTAAATGGAAATGCA	ACGTAGTTGTACAGTGCAATTC	20	22	45.0	40.9	57.9	57.0	0.0	7.4	3.121343	1388	4048	2822-2822	1.000000	55.1
matK-trnK-UUU_p1	matK-trnK-UUU	1	TAGGAAGTGTTGTTGCCGAG	TCTGGGTTGCTAACTCAACG	20	20	50.0	50.0	57.8	57.8	0.0	3.7	0.327636	3228	4011	881-881	1.000000	77.5
matK-trnK-UUU_p2	matK-trnK-UUU	2	TAGGAAGTGTTGTTGCCGAG	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.8	58.2	0.0	17.1	0.332238	3228	4011	865-865	1.000000	77.4
matK-trnK-UUU_p3	matK-trnK-UUU	3	TAGGAAGTGTTGTTGCCGAG	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.8	58.2	0.0	17.1	0.332238	3228	4011	864-864	1.000000	77.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	TAGGAAGTGTTGTTGCCGAG	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	57.8	57.5	0.0	0.0	0.624575	3228	4011	878-878	1.000000	75.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	GGAAGTGTTGTTGCCGAGAT	TCTGGGTTGCTAACTCAACG	20	20	50.0	50.0	58.5	57.8	0.0	3.7	0.640060	3228	4011	879-879	1.000000	75.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5827	6812	1059-1059	1.000000	78.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5827	6812	1070-1070	1.000000	77.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5827	6812	1063-1063	1.000000	73.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	5827	6812	1089-1089	1.000000	73.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.2	58.1	0.0	16.9	0.844354	5827	6812	1071-1071	1.000000	73.3
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	CTGATGGAGACGATCTGGGA	TCGGGCAATTTAGCGAAGAA	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.404786	6885	7229	514-514	1.000000	77.1
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	TCTGATGGAGACGATCTGGG	TCGGGCAATTTAGCGAAGAA	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.404786	6885	7229	515-515	1.000000	77.1
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	CTGATGGAGACGATCTGGGA	CTCGGGCAATTTAGCGAAGA	20	20	55.0	50.0	58.3	58.3	0.0	0.0	0.650729	6885	7229	515-515	1.000000	75.1
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	TCTGATGGAGACGATCTGGG	CTCGGGCAATTTAGCGAAGA	20	20	55.0	50.0	58.3	58.3	0.0	0.0	0.650729	6885	7229	516-516	1.000000	75.1
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	TGATGGAGACGATCTGGGAC	TCGGGCAATTTAGCGAAGAA	20	20	55.0	45.0	58.6	57.9	0.0	0.0	0.698256	6885	7229	513-513	1.000000	74.7
psbK-psbI_p1	psbK-psbI	1	CTTTTGTTTGGCAAGCTGCT	TTACGTCCCGGATCGTTAGA	20	20	45.0	50.0	58.1	58.0	15.2	23.7	0.092926	7416	7863	574-575	1.000000	79.4
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	TTACGTCCCGGATCGTTAGA	20	20	50.0	50.0	57.9	58.0	1.4	23.7	0.093052	7416	7863	569-570	1.000000	79.4
psbK-psbI_p3	psbK-psbI	3	CTTTTGTTTGGCAAGCTGCT	ACGTCCCGGATCGTTAGATA	20	20	45.0	50.0	58.1	57.7	15.2	15.8	0.316812	7416	7863	572-573	1.000000	77.6
psbK-psbI_p4	psbK-psbI	4	CTTTTGTTTGGCAAGCTGCT	TACGTCCCGGATCGTTAGAT	20	20	45.0	50.0	58.1	57.7	15.2	23.7	0.316812	7416	7863	573-574	1.000000	77.6
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCTGCTGTAAG	ACGTCCCGGATCGTTAGATA	20	20	50.0	50.0	57.9	57.7	1.4	15.8	0.316938	7416	7863	567-568	1.000000	77.6
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	GATCAGAGGGAACGGAGAGA	ACGAATCGCACTTTTACCACT	20	21	55.0	42.9	57.9	58.2	0.0	1.2	1.297305	8183	9542	1494-1511	1.000000	69.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AAGTGATCAGAGGGAACGGA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.1	58.2	0.0	1.2	1.298670	8183	9542	1498-1515	1.000000	69.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATCAGAGGGAACGGAGAGAG	ACGAATCGCACTTTTACCACT	20	21	55.0	42.9	57.9	58.2	0.0	1.2	1.303153	8183	9542	1493-1510	1.000000	69.7
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	GATCAGAGGGAACGGAGAGA	ACGAATCGCACTTTTACCAC	20	20	55.0	45.0	57.9	56.8	0.0	1.2	1.304330	8183	9542	1494-1511	1.000000	69.7
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GATCAGAGGGAACGGAGAGA	CGAACGAATCGCACTTTTACC	20	21	55.0	47.6	57.9	58.2	0.0	1.2	1.305572	8183	9542	1497-1514	1.000000	69.7
rpoC1_p1	rpoC1	1	TTGGATAGACCCGTCTTCCC	TTGCTCGTTCGAGAACTACG	20	20	55.0	50.0	58.5	58.0	16.6	23.5	0.547489	21048	23833	2960-2960	1.000000	75.6
rpoC1_p2	rpoC1	2	TTGGATAGACCCGTCTTCCC	GCTCGTTCGAGAACTACGAT	20	20	55.0	50.0	58.5	57.5	16.6	23.5	0.962531	21048	23833	2958-2958	1.000000	72.3
rpoC1_p3	rpoC1	3	GGGCACCGATATCGTCTAAT	TTGCTCGTTCGAGAACTACG	20	20	50.0	50.0	57.0	58.0	22.8	23.5	1.072467	21048	23833	2938-2938	1.000000	71.4
rpoC1_p4	rpoC1	4	GGGCACCGATATCGTCTAATC	TTGCTCGTTCGAGAACTACG	21	20	52.4	50.0	58.1	58.0	22.8	23.5	1.162918	21048	23833	2938-2938	1.000000	70.7
rpoC1_p5	rpoC1	5	GGATAGACCCGTCTTCCCA	TTGCTCGTTCGAGAACTACG	19	20	57.9	50.0	57.8	58.0	2.1	23.5	1.259227	21048	23833	2958-2958	1.000000	70.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	31726	32220	599-599	1.000000	79.0
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	31726	32220	578-578	1.000000	78.5
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	31726	32220	575-575	1.000000	78.4
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	31726	32220	596-596	1.000000	77.8
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.452638	31726	32220	601-601	1.000000	76.8
psbC_p1	psbC	1	GCAGCGGAAGATCCTGAATT	ATAAAGGAGAATGGCTCGGC	20	20	50.0	50.0	58.3	57.7	0.6	0.0	0.589255	34054	35475	1592-1592	1.000000	75.3
psbC_p2	psbC	2	AGGTATTCGTGCTTGGATGG	ATAAAGGAGAATGGCTCGGC	20	20	50.0	50.0	57.7	57.7	0.0	0.0	0.594486	34054	35475	1536-1536	1.000000	75.3
psbC_p3	psbC	3	GCAGCGGAAGATCCTGAATT	AAAGGAGAATGGCTCGGCTA	20	20	50.0	50.0	58.3	58.8	0.6	0.0	1.122686	34054	35475	1590-1590	1.000000	71.1
psbC_p4	psbC	4	GCAGCGGAAGATCCTGAATT	TAAAGGAGAATGGCTCGGCT	20	20	50.0	50.0	58.3	58.8	0.6	0.0	1.122686	34054	35475	1591-1591	1.000000	71.1
psbC_p5	psbC	5	AGGTATTCGTGCTTGGATGG	AAAGGAGAATGGCTCGGCTA	20	20	50.0	50.0	57.7	58.8	0.0	0.0	1.127917	34054	35475	1534-1534	1.000000	71.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AGAGAAAAAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	33.1	0.0	0.063299	46521	47655	1236-1236	1.000000	79.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATAGAGAAAAAGCCGGCT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	27.1	0.0	0.063299	46521	47655	1240-1240	1.000000	79.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TCGATAGAGAAAAAGCCGGC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	21.9	0.0	0.068691	46521	47655	1241-1241	1.000000	79.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AGAGAAAAAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	33.1	0.0	0.128108	46521	47655	1244-1244	1.000000	79.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CGATAGAGAAAAAGCCGGCT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	27.1	0.0	0.128108	46521	47655	1248-1248	1.000000	79.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	ATGCCAGAAAGTTGGATGGG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.195267	48716	49435	835-835	1.000000	78.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CTAGTATGATGCGCGGGAAA	ATGCCAGAAAGTTGGATGGG	20	20	50.0	50.0	57.9	58.1	8.8	0.0	0.292287	48716	49435	896-896	1.000000	77.8
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGAAATGGTCGGGATAGCTC	ATGCCAGAAAGTTGGATGGG	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.314712	48716	49435	881-881	1.000000	77.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AATGGTCGGGATAGCTCAGT	ATGCCAGAAAGTTGGATGGG	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.348657	48716	49435	878-878	1.000000	77.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	ATGCCAGAAAGTTGGATGGG	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.348657	48716	49435	877-877	1.000000	77.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGTCCTTCACTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.423885	51112	52629	1654-1664	1.000000	77.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGTCCTTCACTAATCGGAGC	AGCCCAATGCAACAAAATGC	20	20	55.0	45.0	57.8	58.5	0.0	0.0	0.700661	51112	52629	1736-1746	1.000000	74.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGTCCTTCACTAATCGGAGC	GAAGGTCTACGGTTCGAGTC	20	20	55.0	55.0	57.8	57.5	0.0	0.0	0.767657	51112	52629	1653-1663	1.000000	74.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGTCCTTCACTAATCGGAGC	GTTCGAGTCCGTATAGCCCT	20	20	55.0	55.0	57.8	58.7	0.0	0.0	0.909951	51112	52629	1642-1652	1.000000	73.2
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGTCCTTCACTAATCGGAGC	GAAAGAGCCCAATGCAACAA	20	20	55.0	45.0	57.8	57.2	0.0	0.0	1.044732	51112	52629	1741-1751	1.000000	72.1
petA-psbJ_p1	petA-psbJ	1	GGCATCCGTTATTTTGGCAC	CCGGATTGGGTTCATCTCTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.119715	64140	65245	1214-1214	1.000000	79.1
petA-psbJ_p2	petA-psbJ	2	GGCATCCGTTATTTTGGCAC	TCCGGATTGGGTTCATCTCT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199182	64140	65245	1215-1215	1.000000	78.5
petA-psbJ_p3	petA-psbJ	3	CCCAGGGCCTTTTGTTTTTC	CCGGATTGGGTTCATCTCTG	20	20	50.0	55.0	57.7	58.0	1.3	0.0	0.295504	64140	65245	1236-1236	1.000000	77.7
petA-psbJ_p4	petA-psbJ	4	CCCAGGGCCTTTTGTTTTTC	TCCGGATTGGGTTCATCTCT	20	20	50.0	50.0	57.7	58.1	1.3	0.0	0.374971	64140	65245	1237-1237	1.000000	77.1
petA-psbJ_p5	petA-psbJ	5	TGGCATCCGTTATTTTGGCA	CCGGATTGGGTTCATCTCTG	20	20	45.0	55.0	58.4	58.0	0.0	0.0	0.490632	64140	65245	1215-1215	1.000000	76.1
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGGGCTGGGGTTATAGTTGA	20	20	50.0	50.0	57.9	57.7	6.4	0.0	0.331642	66015	67300	1452-1452	1.000000	77.4
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	AGGGCTGGGGTTATAGTTGA	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.369258	66015	67300	1465-1465	1.000000	77.1
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGGGCTGGGGTTATAGTTGA	20	20	45.0	50.0	58.2	57.7	0.0	0.0	0.451329	66015	67300	1461-1461	1.000000	76.4
psbE-petL_p4	psbE-petL	4	CGAATAACCAACCCGCAATG	AGGGCTGGGGTTATAGTTGA	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.467659	66015	67300	1460-1460	1.000000	76.3
psbE-petL_p5	psbE-petL	5	CAACCCGCAATGAATAGGGA	CAATCAATAGGGCTGGGGTT	20	20	50.0	50.0	57.9	57.5	6.4	0.0	0.510890	66015	67300	1460-1460	1.000000	76.0
psaJ-rpl33_p1	psaJ-rpl33	1	ATATCTTTCCACAGCGCCTG	AGAAATGCCTGGCGATTCTT	20	20	50.0	45.0	58.0	57.9	2.3	24.7	0.175747	68616	69151	741-741	1.000000	78.7
psaJ-rpl33_p2	psaJ-rpl33	2	CATATCTTTCCACAGCGCCT	AGAAATGCCTGGCGATTCTT	20	20	50.0	45.0	58.0	57.9	2.3	24.7	0.175747	68616	69151	742-742	1.000000	78.7
psaJ-rpl33_p3	psaJ-rpl33	3	TGGTTTGGATCTTTAGCGGG	AGAAATGCCTGGCGATTCTT	20	20	50.0	45.0	57.9	57.9	0.0	24.7	0.257572	68616	69151	707-707	1.000000	78.1
psaJ-rpl33_p4	psaJ-rpl33	4	GGTTTGGATCTTTAGCGGGT	AGAAATGCCTGGCGATTCTT	20	20	50.0	45.0	57.9	57.9	0.0	24.7	0.259066	68616	69151	706-706	1.000000	78.0
psaJ-rpl33_p5	psaJ-rpl33	5	ACATATCTTTCCACAGCGCC	AGAAATGCCTGGCGATTCTT	20	20	50.0	45.0	58.3	57.9	2.3	24.7	0.466370	68616	69151	743-743	1.000000	76.4
clpP_p1	clpP	1	TCGAATTCTGATTTGAAACGGA	AACTCATTCTTACGTTTCCA	22	20	36.4	35.0	56.4	52.5	3.5	0.0	9.162895	71471	73534	2157-2157	1.000000	40.0
clpP_p2	clpP	2	TCGAATTCTGATTTGAAACGGA	AAACTCATTCTTACGTTTCCA	22	21	36.4	33.3	56.4	53.4	3.5	0.0	9.223958	71471	73534	2158-2158	1.000000	40.0
clpP_p3	clpP	3	TCGAATTCTGATTTGAAACGGA	ACTCATTCTTACGTTTCCAT	22	20	36.4	35.0	56.4	52.2	3.5	0.0	9.410642	71471	73534	2156-2156	1.000000	40.0
clpP_p4	clpP	4	TCGAATTCTGATTTGAAACGGA	AACTCATTCTTACGTTTCCAT	22	21	36.4	33.3	56.4	53.2	3.5	0.0	9.456468	71471	73534	2157-2157	1.000000	40.0
clpP_p5	clpP	5	TCGAATTCTGATTTGAAACGGAA	AACTCATTCTTACGTTTCCA	23	20	34.8	35.0	57.1	52.5	3.5	0.0	9.480837	71471	73534	2157-2157	1.000000	40.0
rps8-rpl14_p1	rps8-rpl14	1	GTGGATGCTATTCGAACCGT	CGATTGCTCGGGAATTGAGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.124093	82070	82238	323-323	1.000000	79.3
rps8-rpl14_p2	rps8-rpl14	2	GTGGATGCTATTCGAACCGT	TTGCTCGGGAATTGAGACAG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.163209	82070	82238	320-320	1.000000	78.9
rps8-rpl14_p3	rps8-rpl14	3	GTGGATGCTATTCGAACCGT	GCTCGGGAATTGAGACAGTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.164672	82070	82238	318-318	1.000000	78.9
rps8-rpl14_p4	rps8-rpl14	4	AGTGGATGCTATTCGAACCG	CGATTGCTCGGGAATTGAGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.285094	82070	82238	324-324	1.000000	78.0
rps8-rpl14_p5	rps8-rpl14	5	AGTGGATGCTATTCGAACCG	TTGCTCGGGAATTGAGACAG	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.324209	82070	82238	321-321	1.000000	77.7