primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	CTATCGAACGATCCAGGACG	GGGTTCGAATCCCTCTCTCT	20	20	55.0	55.0	57.7	58.2	12.9	17.2	0.534608	7865	7939	131-140	1.000000	82.6
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	ATCGAACGATCCAGGACGTA	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	58.3	58.2	14.9	17.2	0.544950	7865	7939	129-138	1.000000	82.6
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	CTATCGAACGATCCAGGACG	TTTTGGAGAGATGGCTGAGC	20	20	55.0	50.0	57.7	57.9	12.9	0.0	0.437480	7865	7939	200-209	1.000000	81.9
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	ATCGAACGATCCAGGACGTA	TTTTGGAGAGATGGCTGAGC	20	20	50.0	50.0	58.3	57.9	14.9	0.0	0.447822	7865	7939	198-207	1.000000	81.9
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	TATCGAACGATCCAGGACGT	TTTTGGAGAGATGGCTGAGC	20	20	50.0	50.0	58.3	57.9	12.9	0.0	0.447822	7865	7939	199-208	1.000000	81.9
petN-psbM_p1	petN-psbM	1	CGGTATAACTTGGGCTGCTT	ACGCCAAAAAGAATGCTTGA	20	20	50.0	40.0	58.0	56.8	0.0	0.0	1.214043	26752	27495	794-843	1.000000	75.4
petN-psbM_p2	petN-psbM	2	GGGGGAGAAGTGGGCTATAA	ACGCCAAAAAGAATGCTTGA	20	20	55.0	40.0	58.2	56.8	0.0	0.0	1.359899	26752	27495	735-784	1.000000	74.3
petN-psbM_p3	petN-psbM	3	CGGTATAACTTGGGCTGCTT	CCCTTACGCCAAAAAGAATGC	20	21	50.0	47.6	58.0	58.4	0.0	0.0	1.432279	26752	27495	799-848	1.000000	73.6
petN-psbM_p4	petN-psbM	4	CGGTATAACTTGGGCTGCTT	TCGTTGGATTTCCTACTGCT	20	20	50.0	45.0	58.0	56.6	0.0	9.1	1.481758	26752	27495	830-879	1.000000	73.2
petN-psbM_p5	petN-psbM	5	ACTTGGGCTGCTTTAATGGT	ACGCCAAAAAGAATGCTTGA	20	20	45.0	40.0	57.7	56.8	0.0	0.0	1.484793	26752	27495	787-836	1.000000	73.2
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TCAAGCATTCTTTTTGGCGT	TTAGAGTACCGCCCTGTCAA	20	20	40.0	50.0	56.8	58.1	0.0	0.0	1.248169	27592	28529	1008-1094	1.000000	75.1
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ACTGCTTGCAGTGCTTCAA	TTAGAGTACCGCCCTGTCAA	19	20	47.4	50.0	58.2	58.1	25.0	0.0	1.274676	27592	28529	1023-1109	1.000000	74.8
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TCAAGCATTCTTTTTGGCGT	ATTGGTTAGAGTACCGCCCT	20	20	40.0	50.0	56.8	58.1	0.0	0.0	1.300168	27592	28529	1013-1099	1.000000	74.6
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	ACTGCTTGCAGTGCTTCAA	ATTGGTTAGAGTACCGCCCT	19	20	47.4	50.0	58.2	58.1	25.0	0.0	1.326675	27592	28529	1028-1114	1.000000	74.4
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	ACTGCTTGCAGTGCTTCAAG	TTAGAGTACCGCCCTGTCAA	20	20	50.0	50.0	59.3	58.1	25.0	0.0	1.405600	27592	28529	1023-1109	1.000000	73.8
trnT-GGU-trnH-GUG_p1	trnT-GGU-trnH-GUG	1	GCCCTTTTAACTCAGCGGTA	CCAAGGCAGTGGATTGTGAA	20	20	50.0	50.0	57.9	58.4	0.0	1.1	0.483883	29887	30009	249-251	1.000000	78.4
trnT-GGU-trnH-GUG_p2	trnT-GGU-trnH-GUG	2	GGGAGGCCCTTTTAACTCAG	CCAAGGCAGTGGATTGTGAA	20	20	55.0	50.0	57.9	58.4	0.0	1.1	0.510618	29887	30009	254-256	1.000000	78.1
trnT-GGU-trnH-GUG_p3	trnT-GGU-trnH-GUG	3	GCCCTTTTAACTCAGCGGTA	AGTTGTTCGGTTGGTTTCCA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.235313	29887	30009	290-291	0.800000	71.5
trnT-GGU-trnH-GUG_p4	trnT-GGU-trnH-GUG	4	GGGAGGCCCTTTTAACTCAG	AGTTGTTCGGTTGGTTTCCA	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.262048	29887	30009	295-296	0.800000	71.3
trnT-GGU-trnH-GUG_p5	trnT-GGU-trnH-GUG	5	GGCGTAAGTCATCGGTTCAA	AGTTGTTCGGTTGGTTTCCA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.414582	29887	30009	253-254	0.800000	70.3
trnH-GUG-trnV-UAC_p1	trnH-GUG-trnV-UAC	1	TTCACAATCCACTGCCTTGG	CATGTTGGGTTCCTGGAACA	20	20	50.0	50.0	58.4	58.0	0.0	33.7	0.386390	30085	31756	1442-1850	1.000000	82.4
trnH-GUG-trnV-UAC_p2	trnH-GUG-trnV-UAC	2	TTCACAATCCACTGCCTTGG	TGCGAGAAAATGCATGTTGG	20	20	50.0	45.0	58.4	57.6	0.0	6.9	0.731245	30085	31756	1454-1862	1.000000	79.6
trnH-GUG-trnV-UAC_p3	trnH-GUG-trnV-UAC	3	TTCACAATCCACTGCCTTGG	GCGAGAAAATGCATGTTGGG	20	20	50.0	50.0	58.4	58.4	0.0	6.9	0.747924	30085	31756	1453-1861	1.000000	79.5
trnH-GUG-trnV-UAC_p4	trnH-GUG-trnV-UAC	4	CGTGTGGTGGATTCACAATC	CATGTTGGGTTCCTGGAACA	20	20	50.0	50.0	57.1	58.0	6.4	33.7	0.906166	30085	31756	1453-1861	1.000000	78.2
trnH-GUG-trnV-UAC_p5	trnH-GUG-trnV-UAC	5	ATTTTATGGGCGAACGACGG	CATGTTGGGTTCCTGGAACA	20	20	50.0	50.0	59.0	58.0	0.0	33.7	0.994711	30085	31756	1485-1893	1.000000	77.5
rbcL-accD_p1	rbcL-accD	1	GAGTCCTGAACTAGCTGCTG	CTACGCTTGTCTTCGAACCA	20	20	55.0	50.0	57.7	57.9	13.4	0.0	0.352429	37037	37662	737-777	1.000000	82.4
rbcL-accD_p2	rbcL-accD	2	GAGTCCTGAACTAGCTGCTG	TACGCTTGTCTTCGAACCAC	20	20	55.0	50.0	57.7	58.2	13.4	0.0	0.517464	37037	37662	736-776	1.000000	81.1
rbcL-accD_p3	rbcL-accD	3	GCTGCTGCTTGTGAAGTATG	CTACGCTTGTCTTCGAACCA	20	20	50.0	50.0	57.5	57.9	8.5	0.0	0.599672	37037	37662	724-764	1.000000	80.5
rbcL-accD_p4	rbcL-accD	4	GAGTCCTGAACTAGCTGCTG	TCTACGCTTGTCTTCGAACC	20	20	55.0	50.0	57.7	57.7	13.4	0.0	0.635013	37037	37662	738-778	1.000000	80.1
rbcL-accD_p5	rbcL-accD	5	GCTGCTGCTTGTGAAGTATG	TACGCTTGTCTTCGAACCAC	20	20	50.0	50.0	57.5	58.2	8.5	0.0	0.764707	37037	37662	723-763	1.000000	79.2
accD_p1	accD	1	AAGCCAACAAAATTTTCCCT	CCCGAGTTCAACAATGAGGA	20	20	35.0	50.0	54.1	57.5	0.0	0.0	4.378282	37663	38919	1352-1418	1.000000	48.2
accD_p2	accD	2	AAGCCAACAAAATTTTCCCT	TCCCGAGTTCAACAATGAGG	20	20	35.0	50.0	54.1	57.5	0.0	0.0	4.378282	37663	38919	1353-1419	1.000000	48.2
accD_p3	accD	3	AGCCAACAAAATTTTCCCTT	CCCGAGTTCAACAATGAGGA	20	20	35.0	50.0	54.1	57.5	0.0	0.0	4.378282	37663	38919	1351-1417	1.000000	48.2
accD_p4	accD	4	AGCCAACAAAATTTTCCCTT	TCCCGAGTTCAACAATGAGG	20	20	35.0	50.0	54.1	57.5	0.0	0.0	4.378282	37663	38919	1352-1418	1.000000	48.2
accD_p5	accD	5	AAGCCAACAAAATTTTCCCTT	CCCGAGTTCAACAATGAGGA	21	20	33.3	50.0	55.0	57.5	0.0	0.0	4.504400	37663	38919	1352-1418	1.000000	47.2
petA-psbJ_p1	petA-psbJ	1	AGTTCAATTGGCCGAGATGA	TCGTGATTGGTCTACTGGGT	20	20	45.0	50.0	57.2	58.1	5.4	0.0	0.854451	43212	43930	793-813	1.000000	77.5
petA-psbJ_p2	petA-psbJ	2	AGTTCAATTGGCCGAGATGA	TGGAAGGATCCCTCTTTGGT	20	20	45.0	50.0	57.2	57.9	5.4	8.6	0.847229	43212	43930	837-857	1.000000	77.4
petA-psbJ_p3	petA-psbJ	3	AGTTCAATTGGCCGAGATGA	TACCACTGGAAGGATCCCTC	20	20	45.0	55.0	57.2	57.8	5.4	8.6	0.963720	43212	43930	843-863	1.000000	76.4
petA-psbJ_p4	petA-psbJ	4	AAGTTCAATTGGCCGAGATGA	TGGAAGGATCCCTCTTTGGT	21	20	42.9	50.0	57.9	57.9	5.4	8.6	1.131630	43212	43930	838-858	1.000000	75.1
petA-psbJ_p5	petA-psbJ	5	AGTTCAATTGGCCGAGATGAA	TGGAAGGATCCCTCTTTGGT	21	20	42.9	50.0	57.9	57.9	16.4	8.6	1.131630	43212	43930	837-857	1.000000	75.1
trnF-GAA-trnL-UAA_p1	trnF-GAA-trnL-UAA	1	TGGCACGAGGATTTTCAGTC	CCGTTGGCTTTATAGACCGT	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.496341	66361	66746	377-480	1.000000	82.3
trnF-GAA-trnL-UAA_p2	trnF-GAA-trnL-UAA	2	TGGCACGAGGATTTTCAGTC	TAGGAGGAAAATCCGTTGGC	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.604899	66361	66746	389-492	1.000000	81.4
trnF-GAA-trnL-UAA_p3	trnF-GAA-trnL-UAA	3	TGGCACGAGGATTTTCAGTC	GGAGGAAAATCCGTTGGCTT	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.645748	66361	66746	387-490	1.000000	81.1
trnF-GAA-trnL-UAA_p4	trnF-GAA-trnL-UAA	4	CCAGATTTGAACTGGTGGCA	CCGTTGGCTTTATAGACCGT	20	20	50.0	50.0	58.4	57.7	0.0	0.0	0.685097	66361	66746	392-495	1.000000	80.8
trnF-GAA-trnL-UAA_p5	trnF-GAA-trnL-UAA	5	GTGGCACGAGGATTTTCAGT	CCGTTGGCTTTATAGACCGT	20	20	50.0	50.0	58.5	57.7	0.0	0.0	0.780994	66361	66746	378-481	1.000000	80.1
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	ATTCGATCAAGTCCGTAGCG	CAAGTGCGATGCTCTAACCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.136852	67315	67738	433-502	1.000000	83.4
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	CCGTAGCGTCTACCAATTCC	CAAGTGCGATGCTCTAACCT	20	20	55.0	50.0	58.2	58.0	10.2	0.0	0.166639	67315	67738	421-490	1.000000	83.2
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	ATTCGATCAAGTCCGTAGCG	GAACGGAAAAGAGCCAGCTA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.215878	67315	67738	482-551	1.000000	82.7
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	ATTCGATCAAGTCCGTAGCG	GCATTACAAGTGCGATGCTC	20	20	50.0	50.0	57.9	57.9	0.0	10.5	0.217915	67315	67738	439-508	1.000000	82.7
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	ATTCGATCAAGTCCGTAGCG	ATGGGAATGAGAGGAACGGA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.241541	67315	67738	495-564	1.000000	82.5
trnS-GGA-ycf3_p1	trnS-GGA-ycf3	1	GAACCCTCGGTAAGCAGAAG	CGGAGCCATCGGGATTATTC	20	20	55.0	55.0	58.0	58.3	0.0	0.0	0.284687	69028	69769	890-902	1.000000	80.3
trnS-GGA-ycf3_p2	trnS-GGA-ycf3	2	GAACCCTCGGTAAGCAGAAG	GGAGCCATCGGGATTATTCG	20	20	55.0	55.0	58.0	58.3	0.0	0.0	0.284687	69028	69769	889-901	1.000000	80.3
trnS-GGA-ycf3_p3	trnS-GGA-ycf3	3	ACGGAAAGAGAGGGATTCGA	CGGAGCCATCGGGATTATTC	20	20	50.0	55.0	58.2	58.3	0.0	0.0	0.424495	69028	69769	908-920	1.000000	79.1
trnS-GGA-ycf3_p4	trnS-GGA-ycf3	4	ACGGAAAGAGAGGGATTCGA	GGAGCCATCGGGATTATTCG	20	20	50.0	55.0	58.2	58.3	0.0	0.0	0.424495	69028	69769	907-919	1.000000	79.1
trnS-GGA-ycf3_p5	trnS-GGA-ycf3	5	TTCCAATGCTACGCCTTGAA	GGAGCCATCGGGATTATTCG	20	20	45.0	55.0	57.8	58.3	5.6	0.0	0.465810	69028	69769	857-869	1.000000	79.0
ycf3-psaA_p1	ycf3-psaA	1	CTCCGGGCAATTACATCGAA	CCAGGTTTGGCCCATTTTTC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.235374	71712	72566	943-1018	1.000000	84.0
ycf3-psaA_p2	ycf3-psaA	2	TCCGGGCAATTACATCGAAG	CCAGGTTTGGCCCATTTTTC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.235374	71712	72566	942-1017	1.000000	84.0
ycf3-psaA_p3	ycf3-psaA	3	GCTGCTGAGTATTGGGAACA	CCAGGTTTGGCCCATTTTTC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.297338	71712	72566	977-1052	1.000000	83.4
ycf3-psaA_p4	ycf3-psaA	4	CTCCGGGCAATTACATCGAA	CGGCCTTTACGTCTAGTTGT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.410835	71712	72566	891-966	1.000000	82.7
ycf3-psaA_p5	ycf3-psaA	5	TCCGGGCAATTACATCGAAG	CGGCCTTTACGTCTAGTTGT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.410835	71712	72566	890-965	1.000000	82.7
ycf1_p1	ycf1	1	AGGAGGAAGAACGGGAAGAA	TGGTTCGGAGACCACTACTT	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.031006	99600	99719	563-611	1.000000	86.8
ycf1_p2	ycf1	2	CCGGAAAAAGATGGAGGAGG	TGGTTCGGAGACCACTACTT	20	20	55.0	50.0	58.0	58.0	0.0	7.6	0.049493	99600	99719	580-628	1.000000	86.6
ycf1_p3	ycf1	3	GGGATTATGGAGTCTCCGGA	TGGTTCGGAGACCACTACTT	20	20	55.0	50.0	58.0	58.0	14.3	7.6	0.016493	99600	99719	765-825	1.000000	86.4
ycf1_p4	ycf1	4	GGGATTATGGAGTCTCCGGA	TTCTCTGCGACGTTTTGGAA	20	20	55.0	45.0	58.0	58.1	14.3	0.0	0.068445	99600	99719	972-1032	1.000000	85.6
ycf1_p5	ycf1	5	TCGTGAATAGTCCTCGCTCT	TGGTTCGGAGACCACTACTT	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.045188	99600	99719	908-968	0.800000	77.7
rpl32-trnV-GAC_p1	rpl32-trnV-GAC	1	GTACGCTTTTTCGGAACTGC	TTGACGTGGTGGAAGTCATC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.217297	107251	108301	1016-1116	1.000000	83.4
rpl32-trnV-GAC_p2	rpl32-trnV-GAC	2	GTACGCTTTTTCGGAACTGC	CGTGGTGGAAGTCATCAGTT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.223311	107251	108301	1012-1112	1.000000	83.4
rpl32-trnV-GAC_p3	rpl32-trnV-GAC	3	GTACGCTTTTTCGGAACTGC	CTTGACGTGGTGGAAGTCAT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.223311	107251	108301	1017-1117	1.000000	83.4
rpl32-trnV-GAC_p4	rpl32-trnV-GAC	4	AAAAGCTTTTATTGCGGCCC	TTGACGTGGTGGAAGTCATC	20	20	45.0	50.0	57.9	57.8	22.7	0.0	0.257124	107251	108301	1089-1189	1.000000	83.0
rpl32-trnV-GAC_p5	rpl32-trnV-GAC	5	AAAAGCTTTTATTGCGGCCC	CGTGGTGGAAGTCATCAGTT	20	20	45.0	50.0	57.9	57.8	22.7	0.0	0.263139	107251	108301	1085-1185	1.000000	83.0
rps7-trnL-CAA_p1	rps7-trnL-CAA	1	GCAGAGGCCAATAGAGCTTT	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.0	58.0	5.0	5.8	0.057719	110959	112725	1764-1878	1.000000	85.1
rps7-trnL-CAA_p2	rps7-trnL-CAA	2	GCAGAGGCCAATAGAGCTTT	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	58.0	58.0	5.0	5.8	0.059089	110959	112725	1762-1876	1.000000	85.1
rps7-trnL-CAA_p3	rps7-trnL-CAA	3	GCAGAGGCCAATAGAGCTTT	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	58.0	58.2	5.0	0.0	0.246554	110959	112725	1759-1873	1.000000	83.6
rps7-trnL-CAA_p4	rps7-trnL-CAA	4	GCAGAGGCCAATAGAGCTTT	TCTCGCGTGTCTACCATTTC	20	20	50.0	50.0	58.0	57.7	5.0	5.8	0.323258	110959	112725	1763-1877	1.000000	83.0
rps7-trnL-CAA_p5	rps7-trnL-CAA	5	GCAGAGGCCAATAGAGCTTT	TCGCGTGTCTACCATTTCAC	20	20	50.0	50.0	58.0	58.3	5.0	5.8	0.344632	110959	112725	1761-1875	1.000000	82.8
ycf2-trnH-GUG_p1	ycf2-trnH-GUG	1	TTTATAAGGCGCTCCCGATG	GTAGTTCGGGACCCACATTC	20	20	50.0	55.0	57.8	58.0	7.6	12.2	0.183766	119846	120281	683-863	1.000000	84.5
ycf2-trnH-GUG_p2	ycf2-trnH-GUG	2	TTTATAAGGCGCTCCCGATG	TAGTTCGGGACCCACATTCT	20	20	50.0	50.0	57.8	58.1	7.6	12.2	0.216833	119846	120281	682-862	1.000000	84.3
ycf2-trnH-GUG_p3	ycf2-trnH-GUG	3	TTTATAAGGCGCTCCCGATG	ACATTTGACAGAGGTCCACG	20	20	50.0	50.0	57.8	57.8	7.6	0.0	0.333650	119846	120281	628-808	1.000000	83.2
ycf2-trnH-GUG_p4	ycf2-trnH-GUG	4	TTTATAAGGCGCTCCCGATG	CTTGCTTTGATCCCCTGTCA	20	20	50.0	50.0	57.8	57.8	7.6	0.0	0.368840	119846	120281	564-744	1.000000	82.9
ycf2-trnH-GUG_p5	ycf2-trnH-GUG	5	TTTATAAGGCGCTCCCGATG	TTGCTTTGATCCCCTGTCAG	20	20	50.0	50.0	57.8	57.8	7.6	0.0	0.368840	119846	120281	563-743	1.000000	82.9