primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CCCCACATCAGGCACTAATC TCGAGCCGTATGAGGAGAAA 20 20 55.0 50.0 58.0 58.2 0.0 0.0 0.268192 3917 4498 1209-1210 1.000000 79.2 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 CCCCACATCAGGCACTAATC TTCGAGCCGTATGAGGAGAA 20 20 55.0 50.0 58.0 58.2 0.0 0.0 0.268192 3917 4498 1210-1211 1.000000 79.2 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 CCCCACATCAGGCACTAATC TTTCGAGCCGTATGAGGAGA 20 20 55.0 50.0 58.0 58.2 0.0 0.0 0.268192 3917 4498 1211-1212 1.000000 79.2 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 TGAGGATCAGTCGTGGTCTT TTCGAGCCGTATGAGGAGAA 20 20 50.0 50.0 58.1 58.2 0.0 0.0 0.316333 3917 4498 1002-1004 1.000000 78.9 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 TGAGGATCAGTCGTGGTCTT TCGAGCCGTATGAGGAGAAA 20 20 50.0 50.0 58.1 58.2 0.0 0.0 0.316333 3917 4498 1001-1003 1.000000 78.8 trnS-GCU-psbD_p1 trnS-GCU-psbD 1 ATTAGCAATCCGCCGCTTTA GCCGGACCATCCTACAAAAA 20 20 45.0 50.0 58.0 58.2 0.0 5.0 0.209227 8096 9087 1133-1137 1.000000 80.2 trnS-GCU-psbD_p2 trnS-GCU-psbD 2 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 20 20 45.0 45.0 58.0 58.2 0.0 0.0 0.284276 8096 9087 1120-1124 1.000000 79.6 trnS-GCU-psbD_p3 trnS-GCU-psbD 3 GCTTTAGTCCACTCAGCCAT GCCGGACCATCCTACAAAAA 20 20 50.0 50.0 57.6 58.2 0.9 5.0 0.580969 8096 9087 1119-1123 1.000000 77.2 trnS-GCU-psbD_p4 trnS-GCU-psbD 4 ATTAGCAATCCGCCGCTTTA GAAACGGTCCCTTCGTAACC 20 20 45.0 55.0 58.0 58.6 0.0 0.0 0.605692 8096 9087 1112-1116 1.000000 77.0 trnS-GCU-psbD_p5 trnS-GCU-psbD 5 GCTTTAGTCCACTCAGCCAT ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 57.6 58.2 0.9 0.0 0.656018 8096 9087 1106-1110 1.000000 76.6 psbC-trnS-UGA_p1 psbC-trnS-UGA 1 CATTTGTGGCATGCAGGAAG GAGAGATGGCTGAGTGGTTG 20 20 50.0 55.0 58.0 58.0 21.4 0.0 0.025938 11519 11666 319-337 1.000000 84.5 psbC-trnS-UGA_p2 psbC-trnS-UGA 2 CATTTGTGGCATGCAGGAAG GATTGGGCTGTGGAGAGATG 20 20 50.0 55.0 58.0 58.0 21.4 0.0 0.040030 11519 11666 331-349 1.000000 84.4 psbC-trnS-UGA_p3 psbC-trnS-UGA 3 CATTTGTGGCATGCAGGAAG GCTGAGTGGTTGATAGCTCC 20 20 50.0 55.0 58.0 58.1 21.4 0.0 0.057838 11519 11666 311-329 1.000000 84.3 psbC-trnS-UGA_p4 psbC-trnS-UGA 4 CATTTGTGGCATGCAGGAAG GGCTGAGTGGTTGATAGCTC 20 20 50.0 55.0 58.0 58.1 21.4 0.0 0.057838 11519 11666 312-330 1.000000 84.3 psbC-trnS-UGA_p5 psbC-trnS-UGA 5 CATTTGTGGCATGCAGGAAG GGTTGATAGCTCCGGTCTTG 20 20 50.0 55.0 58.0 58.1 21.4 0.0 0.063258 11519 11666 304-322 1.000000 84.3 trnG-UCC-trnM-CAU_p1 trnG-UCC-trnM-CAU 1 ACGAATCACACTTTTACCACT ATTCGAACCGACGACTTCTG 21 20 38.1 50.0 55.5 58.0 0.0 0.0 3.557301 13771 14620 993-1004 1.000000 54.1 trnG-UCC-trnM-CAU_p2 trnG-UCC-trnM-CAU 2 AGAACGAATCACACTTTTACCAC ATTCGAACCGACGACTTCTG 23 20 39.1 50.0 57.3 58.0 0.0 0.0 3.690016 13771 14620 996-1007 1.000000 53.0 trnG-UCC-trnM-CAU_p3 trnG-UCC-trnM-CAU 3 GAACGAATCACACTTTTACCACT ATTCGAACCGACGACTTCTG 23 20 39.1 50.0 57.3 58.0 0.0 0.0 3.690016 13771 14620 995-1006 1.000000 53.0 trnG-UCC-trnM-CAU_p4 trnG-UCC-trnM-CAU 4 ACGAATCACACTTTTACCACT CCGACGACTTCTGTCTTACC 21 20 38.1 55.0 55.5 57.7 0.0 0.0 3.795347 13771 14620 986-997 1.000000 52.2 trnG-UCC-trnM-CAU_p5 trnG-UCC-trnM-CAU 5 AACGAATCACACTTTTACCACT ATTCGAACCGACGACTTCTG 22 20 36.4 50.0 56.2 58.0 0.0 0.0 3.797771 13771 14620 994-1005 1.000000 52.2 trnY-GUA-trnD-GUC_p1 trnY-GUA-trnD-GUC 1 AATTCTTCCTGGGTCGATGC CGGTGCTCTGACCAATTGAA 20 20 50.0 50.0 58.0 58.5 0.0 7.1 0.521454 15442 15789 470-472 1.000000 77.7 trnY-GUA-trnD-GUC_p2 trnY-GUA-trnD-GUC 2 GGGGACGGACTGTAAATTCG CGGTGCTCTGACCAATTGAA 20 20 55.0 50.0 58.4 58.5 0.0 7.1 0.828828 15442 15789 437-439 1.000000 75.4 trnY-GUA-trnD-GUC_p3 trnY-GUA-trnD-GUC 3 GGGACGGACTGTAAATTCGT CGGTGCTCTGACCAATTGAA 20 20 50.0 50.0 57.6 58.5 0.0 7.1 0.852505 15442 15789 436-438 1.000000 75.2 trnY-GUA-trnD-GUC_p4 trnY-GUA-trnD-GUC 4 AATTCTTCCTGGGTCGATGC GGTGCTCTGACCAATTGAAC 20 20 50.0 50.0 58.0 57.0 0.0 7.1 1.041608 15442 15789 469-471 1.000000 73.5 trnY-GUA-trnD-GUC_p5 trnY-GUA-trnD-GUC 5 TGGGGACGGACTGTAAATTC CGGTGCTCTGACCAATTGAA 20 20 50.0 50.0 57.2 58.5 0.0 7.1 1.251324 15442 15789 438-440 1.000000 72.0 trnD-GUC-psbM_p1 trnD-GUC-psbM 1 CTTAGAACACCGCAGACGAA TCTCCCCTTTCGTGGATTCT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.107704 15864 16791 1496-1620 1.000000 84.4 trnD-GUC-psbM_p2 trnD-GUC-psbM 2 TTCTTAGAACACCGCAGACG TCTCCCCTTTCGTGGATTCT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.107704 15864 16791 1498-1622 1.000000 84.4 trnD-GUC-psbM_p3 trnD-GUC-psbM 3 AATTCTTCCTGGGTCGATGC TCTCCCCTTTCGTGGATTCT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.091501 15864 16791 1862-1985 1.000000 84.0 trnD-GUC-psbM_p4 trnD-GUC-psbM 4 CGCTATCGTCTAGTGATGCC TCTCCCCTTTCGTGGATTCT 20 20 55.0 50.0 58.0 58.0 9.5 0.0 0.076932 15864 16791 2003-2126 1.000000 83.8 trnD-GUC-psbM_p5 trnD-GUC-psbM 5 GGCGCTATCGTCTAGTGATG TCTCCCCTTTCGTGGATTCT 20 20 55.0 50.0 58.0 58.0 7.6 0.0 0.076932 15864 16791 2005-2128 1.000000 83.8 psbM-petN_p1 psbM-petN 1 AAAACAGCCAGCCAAAATGA TAGTAAGTCTCGCTTGGGCT 20 20 40.0 50.0 56.7 58.2 0.0 0.0 1.449068 16897 17684 779-895 1.000000 73.6 psbM-petN_p2 psbM-petN 2 AAAACAGCCAGCCAAAATGA GTAGTATGGGGGAGGAGTGG 20 20 40.0 60.0 56.7 58.3 0.0 0.0 1.579440 16897 17684 723-839 1.000000 72.7 psbM-petN_p3 psbM-petN 3 AAAACAGCCAGCCAAAATGA TAGTATGGGGGAGGAGTGGA 20 20 40.0 55.0 56.7 58.4 0.0 0.0 1.669873 16897 17684 722-838 1.000000 72.0 psbM-petN_p4 psbM-petN 4 AAACAGCCAGCCAAAATGAT TAGTAAGTCTCGCTTGGGCT 20 20 40.0 50.0 56.5 58.2 0.0 0.0 1.680884 16897 17684 778-894 1.000000 71.8 psbM-petN_p5 psbM-petN 5 AACAGCCAGCCAAAATGATT TAGTAAGTCTCGCTTGGGCT 20 20 40.0 50.0 56.5 58.2 0.0 0.0 1.680884 16897 17684 777-893 1.000000 71.8 psaA-ycf3_p1 psaA-ycf3 1 CCGCCTACATCACCATTCAA TAGAGCCGATCCACTAAGGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.040159 41975 42625 1614-1620 1.000000 80.8 psaA-ycf3_p2 psaA-ycf3 2 GCATCAGCATGTAGGTTCCA TAGAGCCGATCCACTAAGGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.045888 41975 42625 1383-1389 1.000000 80.8 psaA-ycf3_p3 psaA-ycf3 3 GTTATTCCAGATGCTCGCCA TAGAGCCGATCCACTAAGGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.049643 41975 42625 1677-1683 1.000000 80.7 psaA-ycf3_p4 psaA-ycf3 4 CCAAGTCCTAATAACCCCGC TAGAGCCGATCCACTAAGGG 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.040030 41975 42625 1842-1848 1.000000 80.6 psaA-ycf3_p5 psaA-ycf3 5 CTACATCTTGGAACCAGGCC TAGAGCCGATCCACTAAGGG 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.047324 41975 42625 1799-1805 1.000000 80.6 ycf3-trnS-GGA_p1 ycf3-trnS-GGA 1 TTGGCTACAATCGAGGAGGT ACGGAAAGAGAGGGATTCGA 20 20 50.0 50.0 58.4 58.2 0.0 0.0 0.582844 44614 45200 725-734 1.000000 77.5 ycf3-trnS-GGA_p2 ycf3-trnS-GGA 2 TTGGCTACAATCGAGGAGGT TTCCAATGCTACGCCTTGAA 20 20 50.0 45.0 58.4 57.8 0.0 5.6 0.624160 44614 45200 675-684 1.000000 77.4 ycf3-trnS-GGA_p3 ycf3-trnS-GGA 3 GGCTACAATCGAGGAGGTCT ACGGAAAGAGAGGGATTCGA 20 20 55.0 50.0 58.6 58.2 0.0 0.0 0.746506 44614 45200 723-732 1.000000 76.2 ycf3-trnS-GGA_p4 ycf3-trnS-GGA 4 GGCTACAATCGAGGAGGTCT TTCCAATGCTACGCCTTGAA 20 20 55.0 45.0 58.6 57.8 0.0 5.6 0.787821 44614 45200 673-682 1.000000 76.1 ycf3-trnS-GGA_p5 ycf3-trnS-GGA 5 TTGGCTACAATCGAGGAGGT AGTTCCAATGCTACGCCTTG 20 20 50.0 50.0 58.4 58.5 0.0 0.0 0.978341 44614 45200 677-686 1.000000 74.5 rpl16-rps3_p1 rpl16-rps3 1 AACGAATCACACACTAAGCA TACCCCTCCAAACAATTCGC 20 20 40.0 50.0 55.3 58.2 0.0 0.0 2.842468 79652 79794 271-273 1.000000 58.1 rpl16-rps3_p2 rpl16-rps3 2 ACGAATCACACACTAAGCAT TACCCCTCCAAACAATTCGC 20 20 40.0 50.0 55.1 58.2 0.0 0.0 3.076061 79652 79794 270-272 1.000000 56.3 rpl16-rps3_p3 rpl16-rps3 3 AACGAATCACACACTAAGCAT TACCCCTCCAAACAATTCGC 21 20 38.1 50.0 55.9 58.2 0.0 0.0 3.268350 79652 79794 271-273 1.000000 54.7 rpl16-rps3_p4 rpl16-rps3 4 ACTAACGAATCACACACTAAGCA TACCCCTCCAAACAATTCGC 23 20 39.1 50.0 58.1 58.2 0.0 0.0 3.292581 79652 79794 274-276 1.000000 54.5 rpl16-rps3_p5 rpl16-rps3 5 AACGAATCACACACTAAGCA CTCCAAACAATTCGCGCTAA 20 20 40.0 45.0 55.3 57.1 0.0 5.6 3.563821 79652 79794 266-268 1.000000 52.4 trnH-GUG-rpl2_p1 trnH-GUG-rpl2 1 CTTTAGCTGGATAAGGGGCG GTTATCCTGCGCTTGGAAGA 20 20 55.0 50.0 58.1 58.0 0.0 22.4 0.139940 81545 81599 223-223 1.000000 79.2 trnH-GUG-rpl2_p2 trnH-GUG-rpl2 2 CGGATGTAGCCAAGTGGATC GTTATCCTGCGCTTGGAAGA 20 20 55.0 50.0 58.1 58.0 0.2 22.4 0.153095 81545 81599 205-205 1.000000 79.2 trnH-GUG-rpl2_p3 trnH-GUG-rpl2 3 TGGATCAAGGCAGTGGATTG GTTATCCTGCGCTTGGAAGA 20 20 50.0 50.0 57.9 58.0 0.0 22.4 0.157457 81545 81599 191-191 1.000000 79.2 trnH-GUG-rpl2_p4 trnH-GUG-rpl2 4 GGATCAAGGCAGTGGATTGT GTTATCCTGCGCTTGGAAGA 20 20 50.0 50.0 57.9 58.0 0.0 22.4 0.158965 81545 81599 190-190 1.000000 79.1 trnH-GUG-rpl2_p5 trnH-GUG-rpl2 5 GTGGATCAAGGCAGTGGATT GTTATCCTGCGCTTGGAAGA 20 20 50.0 50.0 57.9 58.0 0.0 22.4 0.158965 81545 81599 192-192 1.000000 79.1 trnN-GUU-rps15_p1 trnN-GUU-rps15 1 ACGACCAGTCAGTTAACAGC AATGTGAAGCAAGTCTCCGT 20 20 50.0 45.0 57.8 57.4 8.6 0.0 0.701227 101803 103383 1721-1721 1.000000 74.8 trnN-GUU-rps15_p2 trnN-GUU-rps15 2 AGGATTCGAACCTACGACCA AATGTGAAGCAAGTCTCCGT 20 20 50.0 45.0 58.2 57.4 15.0 0.0 0.707462 101803 103383 1734-1734 1.000000 74.8 trnN-GUU-rps15_p3 trnN-GUU-rps15 3 GGATTCGAACCTACGACCAG AATGTGAAGCAAGTCTCCGT 20 20 55.0 45.0 57.8 57.4 0.0 0.0 0.756137 101803 103383 1733-1733 1.000000 74.4 trnN-GUU-rps15_p4 trnN-GUU-rps15 4 CCCCAAGTAGGATTCGAACC AATGTGAAGCAAGTCTCCGT 20 20 55.0 45.0 57.7 57.4 0.0 0.0 0.878030 101803 103383 1742-1742 1.000000 73.4 trnN-GUU-rps15_p5 trnN-GUU-rps15 5 TTCGAACCTACGACCAGTCA AATGTGAAGCAAGTCTCCGT 20 20 50.0 45.0 58.4 57.4 0.0 0.0 0.942776 101803 103383 1730-1730 1.000000 72.9 ndhF-rpl32_p1 ndhF-rpl32 1 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGCT 20 24 55.0 33.3 58.1 57.3 0.0 0.0 4.844538 105941 106784 954-955 1.000000 44.0 ndhF-rpl32_p2 ndhF-rpl32 2 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGC 20 23 55.0 34.8 58.1 55.9 0.0 0.0 5.174812 105941 106784 954-955 1.000000 42.8 ndhF-rpl32_p3 ndhF-rpl32 3 GGAAGTGGGAGAAGAGGGAT CCAAAGATTTTTACGAATACGCT 20 23 55.0 34.8 58.1 55.9 0.0 0.0 5.184229 105941 106784 953-954 1.000000 42.8 ndhF-rpl32_p4 ndhF-rpl32 4 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGCTT 20 25 55.0 32.0 58.1 57.9 0.0 0.0 5.249867 105941 106784 954-955 1.000000 42.8 ndhF-rpl32_p5 ndhF-rpl32 5 GGAAGTGGGAGAAGAGGGAT TTCCAAAGATTTTTACGAATACGCT 20 25 55.0 32.0 58.1 57.9 0.0 0.0 5.249867 105941 106784 955-956 1.000000 42.8 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 CAACGAGCATCCAAAACCAA TCCTAAGAGCAGCGTGTCTA 20 20 45.0 50.0 57.2 57.9 0.0 0.0 0.915660 106962 107486 611-613 1.000000 78.6 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 CAACGAGCATCCAAAACCAA AGCAGCGTGTCTACCAATTT 20 20 45.0 45.0 57.2 57.8 0.0 0.0 0.976387 106962 107486 604-606 1.000000 78.1 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 CAACGAGCATCCAAAACCAA CCTAAGAGCAGCGTGTCTAC 20 20 45.0 55.0 57.2 57.8 0.0 0.0 0.987678 106962 107486 610-612 1.000000 78.0 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 CAACGAGCATCCAAAACCAA CTAAGAGCAGCGTGTCTACC 20 20 45.0 55.0 57.2 57.8 0.0 0.0 0.987678 106962 107486 609-611 1.000000 78.0 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 CAACGAGCATCCAAAACCAA GCTTGAGCACTGCTTCCTAA 20 20 45.0 50.0 57.2 58.2 0.0 4.7 0.977870 106962 107486 625-627 1.000000 77.9 ccsA-ndhD_p1 ccsA-ndhD 1 TGGTTCATTTACATTACCATCT TTGACAGGGTAGAGGCTCTC 22 20 31.8 55.0 53.1 58.1 0.0 22.5 7.019519 108619 108806 260-260 1.000000 42.2 ccsA-ndhD_p2 ccsA-ndhD 2 TGGTTCATTTACATTACCATCT GACAGGGTAGAGGCTCTCTT 22 20 31.8 55.0 53.1 57.8 0.0 22.5 7.026515 108619 108806 258-258 1.000000 42.2 ccsA-ndhD_p3 ccsA-ndhD 3 TGGTTCATTTACATTACCATCT CTCTCGCTATCCGTTGACAG 22 20 31.8 55.0 53.1 57.9 0.0 0.0 6.985956 108619 108806 273-273 1.000000 42.1 ccsA-ndhD_p4 ccsA-ndhD 4 TGGTTCATTTACATTACCATCT TCTCTCGCTATCCGTTGACA 22 20 31.8 50.0 53.1 58.3 0.0 0.0 7.127012 108619 108806 274-274 1.000000 42.0 ccsA-ndhD_p5 ccsA-ndhD 5 TGGTTCATTTACATTACCATCT CCGGATTTTGTTCTCTCGCT 22 20 31.8 50.0 53.1 58.3 0.0 0.0 7.146628 108619 108806 285-285 1.000000 42.0 rps15-trnN-GUU_p1 rps15-trnN-GUU 1 AATGTGAAGCAAGTCTCCGT ACGACCAGTCAGTTAACAGC 20 20 45.0 50.0 57.4 57.8 0.0 8.6 0.701227 116742 118322 1721-1721 1.000000 74.8 rps15-trnN-GUU_p2 rps15-trnN-GUU 2 AATGTGAAGCAAGTCTCCGT AGGATTCGAACCTACGACCA 20 20 45.0 50.0 57.4 58.2 0.0 15.0 0.707462 116742 118322 1734-1734 1.000000 74.8 rps15-trnN-GUU_p3 rps15-trnN-GUU 3 AATGTGAAGCAAGTCTCCGT GGATTCGAACCTACGACCAG 20 20 45.0 55.0 57.4 57.8 0.0 0.0 0.756137 116742 118322 1733-1733 1.000000 74.4 rps15-trnN-GUU_p4 rps15-trnN-GUU 4 AATGTGAAGCAAGTCTCCGT CCCCAAGTAGGATTCGAACC 20 20 45.0 55.0 57.4 57.7 0.0 0.0 0.878030 116742 118322 1742-1742 1.000000 73.4 rps15-trnN-GUU_p5 rps15-trnN-GUU 5 AATGTGAAGCAAGTCTCCGT TTCGAACCTACGACCAGTCA 20 20 45.0 50.0 57.4 58.4 0.0 0.0 0.942776 116742 118322 1730-1730 1.000000 72.9 rpl2-trnH-GUG_p1 rpl2-trnH-GUG 1 GTTATCCTGCGCTTGGAAGA CTTTAGCTGGATAAGGGGCG 20 20 50.0 55.0 58.0 58.1 22.4 0.0 0.139940 138526 138580 223-223 1.000000 79.2 rpl2-trnH-GUG_p2 rpl2-trnH-GUG 2 GTTATCCTGCGCTTGGAAGA CGGATGTAGCCAAGTGGATC 20 20 50.0 55.0 58.0 58.1 22.4 0.2 0.153095 138526 138580 205-205 1.000000 79.2 rpl2-trnH-GUG_p3 rpl2-trnH-GUG 3 GTTATCCTGCGCTTGGAAGA TGGATCAAGGCAGTGGATTG 20 20 50.0 50.0 58.0 57.9 22.4 0.0 0.157457 138526 138580 191-191 1.000000 79.2 rpl2-trnH-GUG_p4 rpl2-trnH-GUG 4 GTTATCCTGCGCTTGGAAGA GGATCAAGGCAGTGGATTGT 20 20 50.0 50.0 58.0 57.9 22.4 0.0 0.158965 138526 138580 190-190 1.000000 79.1 rpl2-trnH-GUG_p5 rpl2-trnH-GUG 5 GTTATCCTGCGCTTGGAAGA GTGGATCAAGGCAGTGGATT 20 20 50.0 50.0 58.0 57.9 22.4 0.0 0.158965 138526 138580 192-192 1.000000 79.1