primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TGTCAACCAAATCAGCCCAA	TTTCTGCCTACACTTCGCTC	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.159456	1387	3941	2965-2973	1.000000	80.3
trnK-UUU_p2	trnK-UUU	2	TGTCAACCAAATCAGCCCAA	AGAAGCACCCGGTGTAATTC	20	20	45.0	50.0	57.9	57.9	0.0	18.9	0.188594	1387	3941	2732-2737	0.666667	65.5
trnK-UUU_p3	trnK-UUU	3	GACTCCCAAGCACACGTATT	AGAAGCACCCGGTGTAATTC	20	20	50.0	50.0	57.9	57.9	0.0	18.9	0.204187	1387	3941	2817-2822	0.666667	65.4
trnK-UUU_p4	trnK-UUU	4	TGTCAACCAAATCAGCCCAA	CAGAAGCACCCGGTGTAATT	20	20	45.0	50.0	57.9	58.2	0.0	18.9	0.257953	1387	3941	2733-2738	0.666667	65.0
trnK-UUU_p5	trnK-UUU	5	TGTCAACCAAATCAGCCCAA	GGGTGTCTCTCTTGTTGCAT	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.270297	1387	3941	2944-2944	0.333333	51.2
trnS-GCU-psbD_p1	trnS-GCU-psbD	1	ATTAGCAATCCGCCGCTTTA	GAATCGGTCCCTTCGTAACC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.106300	7649	8620	1096-1100	1.000000	80.2
trnS-GCU-psbD_p2	trnS-GCU-psbD	2	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	20	20	45.0	50.0	58.0	58.2	0.0	5.0	0.209227	7649	8620	1117-1121	1.000000	79.4
trnS-GCU-psbD_p3	trnS-GCU-psbD	3	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAATCGGTCCCT	20	20	45.0	45.0	58.0	57.7	0.0	7.8	0.303795	7649	8620	1104-1108	1.000000	78.7
trnS-GCU-psbD_p4	trnS-GCU-psbD	4	GAGCGGAAAGAGAGGGATTC	GAATCGGTCCCTTCGTAACC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.300850	7649	8620	1147-1151	1.000000	78.6
trnS-GCU-psbD_p5	trnS-GCU-psbD	5	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	20	20	45.0	55.0	58.0	57.7	0.0	0.0	0.349331	7649	8620	1088-1092	1.000000	78.3
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	58.0	58.5	0.0	7.1	0.521454	15564	15916	469-483	1.000000	79.7
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	15564	15916	436-450	1.000000	77.5
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	15564	15916	435-449	1.000000	77.3
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	20	20	50.0	50.0	58.0	57.0	0.0	7.1	1.041608	15564	15916	468-482	1.000000	75.5
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.2	58.5	0.0	7.1	1.251324	15564	15916	437-451	1.000000	74.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GCCCTGAAATCCAAAATGCC	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.104399	49504	50441	1018-1048	1.000000	84.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCTGGTCCTTCACTAGTCGG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.2	57.9	0.0	0.0	0.240485	49504	50441	1046-1076	1.000000	83.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GCCCTGAAATCCAAAATGCC	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	58.0	58.3	0.0	22.8	0.333780	49504	50441	1019-1049	1.000000	82.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CACTAGTCGGGGCTAAAAGC	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.3	57.9	0.0	0.0	0.422828	49504	50441	1036-1066	1.000000	81.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCTGGTCCTTCACTAGTCGG	CGGTTCGAATCCGTATAGCC	20	20	55.0	55.0	58.2	58.3	0.0	22.8	0.469866	49504	50441	1047-1077	1.000000	81.1
rbcL-rpl23_p1	rbcL-rpl23	1	GATCATATCCACGCCGGTAC	CTGTTAGTAAGACCCCGTGC	20	20	55.0	55.0	58.0	58.0	1.9	0.0	0.013801	55623	55877	1409-1437	1.000000	82.2
rbcL-rpl23_p2	rbcL-rpl23	2	ATATCCACGCCGGTACAGTA	CTGTTAGTAAGACCCCGTGC	20	20	50.0	55.0	58.0	58.0	1.9	0.0	0.020497	55623	55877	1405-1433	1.000000	82.2
rbcL-rpl23_p3	rbcL-rpl23	3	TCTTCACATTCACCGTGCAA	CTGTTAGTAAGACCCCGTGC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.045554	55623	55877	1509-1537	1.000000	81.8
rbcL-rpl23_p4	rbcL-rpl23	4	TTCTTCACATTCACCGTGCA	CTGTTAGTAAGACCCCGTGC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.045554	55623	55877	1510-1538	1.000000	81.8
rbcL-rpl23_p5	rbcL-rpl23	5	GCATGACTACATCACTGGGG	CTGTTAGTAAGACCCCGTGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.046929	55623	55877	1581-1609	1.000000	81.7
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	TCCTCTTTGGCTGATAGGTACT	20	22	50.0	45.5	57.9	58.0	0.0	0.0	2.056243	60443	61248	1003-1010	1.000000	65.9
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	CCTCTTTGGCTGATAGGTACTG	20	22	50.0	50.0	57.9	57.9	0.0	0.0	2.117200	60443	61248	1002-1009	1.000000	65.4
petA-psbJ_p3	petA-psbJ	3	TGTTCTTCTTCGCATCCGTT	TCCTCTTTGGCTGATAGGTACT	20	22	45.0	45.5	57.8	58.0	0.0	0.0	2.168182	60443	61248	996-1003	1.000000	65.0
petA-psbJ_p4	petA-psbJ	4	TTGTTCTTCTTCGCATCCGT	TCCTCTTTGGCTGATAGGTACT	20	22	45.0	45.5	57.8	58.0	0.0	0.0	2.168182	60443	61248	997-1004	1.000000	65.0
petA-psbJ_p5	petA-psbJ	5	TGTTCTTCTTCGCATCCGTT	CCTCTTTGGCTGATAGGTACTG	20	22	45.0	50.0	57.8	57.9	0.0	0.0	2.229139	60443	61248	995-1002	1.000000	64.5
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	GCCAATAAATAGAGCTGGGGT	20	21	45.0	47.6	58.0	57.8	0.0	0.0	1.247037	62018	63270	1439-1463	1.000000	71.2
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCCAATAAATAGAGCTGGGGT	20	21	50.0	47.6	57.9	57.8	6.4	0.0	1.281031	62018	63270	1428-1452	1.000000	71.0
psbE-petL_p3	psbE-petL	3	ACCAACCCGCAATGAATAGG	GCCAATAAATAGAGCTGGGGT	20	21	50.0	47.6	58.2	57.8	0.0	0.0	1.466989	62018	63270	1430-1454	1.000000	69.5
psbE-petL_p4	psbE-petL	4	TGACAAATAACCAACCCGCA	AGCCAATAAATAGAGCTGGGG	20	21	45.0	47.6	58.0	57.5	0.0	0.0	1.532941	62018	63270	1440-1464	1.000000	69.0
psbE-petL_p5	psbE-petL	5	CAACCCGCAATGAATAGGGA	AGCCAATAAATAGAGCTGGGG	20	21	50.0	47.6	57.9	57.5	6.4	0.0	1.566936	62018	63270	1429-1453	1.000000	68.7
clpP1-psbB_p1	clpP1-psbB	1	ACCCAAGTCGCTTCTTCATC	TATAAAGCCATCGAGCCAGC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.270437	68078	68611	715-715	1.000000	78.5
clpP1-psbB_p2	clpP1-psbB	2	TCAACCCAAGTCGCTTCTTC	TATAAAGCCATCGAGCCAGC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.290879	68078	68611	718-718	1.000000	78.3
clpP1-psbB_p3	clpP1-psbB	3	ACCCAAGTCGCTTCTTCATC	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.337897	68078	68611	616-616	1.000000	78.1
clpP1-psbB_p4	clpP1-psbB	4	CTTTTGGAACACCAATGGGC	TATAAAGCCATCGAGCCAGC	20	20	50.0	50.0	57.8	57.8	3.6	0.0	0.338158	68078	68611	675-675	1.000000	78.0
clpP1-psbB_p5	clpP1-psbB	5	TCAACCCAAGTCGCTTCTTC	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.358339	68078	68611	619-619	1.000000	77.9
psbT-psbN_p1	psbT-psbN	1	AAGGTTCCACCAACTCCAAC	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	57.9	58.0	0.0	3.3	0.091541	70419	70466	110-110	1.000000	82.9
psbT-psbN_p2	psbT-psbN	2	TTCCGAGAACCACCTAAGGT	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.043983	70419	70466	125-125	1.000000	82.8
psbT-psbN_p3	psbT-psbN	3	TTCCGAGAACCACCTAAGGT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035264	70419	70466	212-212	1.000000	81.6
psbT-psbN_p4	psbT-psbN	4	AAGGTTCCACCAACTCCAAC	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.082822	70419	70466	197-197	1.000000	81.4
psbT-psbN_p5	psbT-psbN	5	CCACCTAAGGTTCCACCAAC	TCGCCATCTCCATATCTGGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.109819	70419	70466	203-203	1.000000	81.1
rps11-rpl36_p1	rps11-rpl36	1	CGTGCACTTTTCCGTAGACT	GCGTGGACGAATTAGAGTCA	20	20	50.0	50.0	58.2	57.7	16.2	2.7	0.493800	75607	75785	329-336	1.000000	77.0
rps11-rpl36_p2	rps11-rpl36	2	TTCTACGTGCACTTTTCCGT	GCGTGGACGAATTAGAGTCA	20	20	45.0	50.0	57.8	57.7	16.2	2.7	0.501507	75607	75785	334-341	1.000000	76.9
rps11-rpl36_p3	rps11-rpl36	3	CGTGCACTTTTCCGTAGACT	GGCGTGGACGAATTAGAGTC	20	20	50.0	55.0	58.2	58.4	16.2	2.7	0.662779	75607	75785	330-337	1.000000	75.7
rps11-rpl36_p4	rps11-rpl36	4	TTCTACGTGCACTTTTCCGT	GGCGTGGACGAATTAGAGTC	20	20	45.0	55.0	57.8	58.4	16.2	2.7	0.670486	75607	75785	335-342	1.000000	75.6
rps11-rpl36_p5	rps11-rpl36	5	CGTGCACTTTTCCGTAGACT	CGTAGGCGTGGACGAATTAG	20	20	50.0	55.0	58.2	58.5	16.2	2.7	0.746414	75607	75785	334-341	1.000000	75.0
rpl16_p1	rpl16	1	CAGAAAGAAAAACAGGGCGT	TCCTGAAATCCCTGGAAAAA	20	20	45.0	40.0	56.8	54.4	0.0	0.0	4.755163	77463	78914	1596-1600	0.666667	29.1
rpl16_p2	rpl16	2	CAGAAAGAAAAACAGGGCGT	TTCCTGAAATCCCTGGAAAA	20	20	45.0	40.0	56.8	54.4	0.0	0.0	4.755163	77463	78914	1597-1601	0.666667	29.1
rpl16_p3	rpl16	3	CAGAAAGAAAAACAGGGCGT	TTTCCTGAAATCCCTGGAAA	20	20	45.0	40.0	56.8	54.4	0.0	9.8	4.755163	77463	78914	1598-1602	0.666667	29.1
rpl16_p4	rpl16	4	CAGAAAGAAAAACAGGGCGT	TTTTCCTGAAATCCCTGGAA	20	20	45.0	40.0	56.8	54.4	0.0	0.0	4.755163	77463	78914	1599-1603	0.666667	29.1
rpl16_p5	rpl16	5	CAGAAAGAAAAACAGGGCGT	TTTTTCCTGAAATCCCTGGA	20	20	45.0	40.0	56.8	54.4	0.0	0.0	4.755163	77463	78914	1600-1604	0.666667	29.1
rpl22-rps19_p1	rpl22-rps19	1	ACTTTACTAGTTTGAAACTTGTCA	CAAATTGGGGGAATTCGTGC	24	20	29.2	50.0	54.6	58.0	24.1	0.0	7.384506	80291	80365	164-182	1.000000	45.4
rpl22-rps19_p2	rpl22-rps19	2	ACTTTACTAGTTTGAAACTTGTCA	GCAAATTGGGGGAATTCGTG	24	20	29.2	50.0	54.6	58.0	24.1	0.0	7.384506	80291	80365	165-183	1.000000	45.4
rpl22-rps19_p3	rpl22-rps19	3	ACTTTACTAGTTTGAAACTTGTCAT	CAAATTGGGGGAATTCGTGC	25	20	28.0	50.0	55.1	58.0	24.1	0.0	7.867333	80291	80365	164-182	1.000000	45.4
rpl22-rps19_p4	rpl22-rps19	4	ACTTTACTAGTTTGAAACTTGTCA	ATGGTAGGTCGCAAATTGGG	24	20	29.2	50.0	54.6	58.2	24.1	3.4	7.613047	80291	80365	175-193	1.000000	45.3
rpl22-rps19_p5	rpl22-rps19	5	ACTTTACTAGTTTGAAACTTGTCA	GTCGCAAATTGGGGGAATTC	24	20	29.2	50.0	54.6	57.7	24.1	10.2	7.669431	80291	80365	168-186	1.000000	45.3
ndhF_p1	ndhF	1	AAAATCGCGGAAAACCGAAC	GGTATGGAAGCCTATCCCCT	20	20	45.0	55.0	58.0	58.0	6.1	0.0	0.086994	102036	104255	2752-2919	1.000000	85.0
ndhF_p2	ndhF	2	AATCGCGGAAAACCGAACTA	GGTATGGAAGCCTATCCCCT	20	20	45.0	55.0	57.9	58.0	6.1	0.0	0.129447	102036	104255	2750-2917	1.000000	84.7
ndhF_p3	ndhF	3	TCGCGGAAAACCGAACTATT	GGTATGGAAGCCTATCCCCT	20	20	45.0	55.0	57.9	58.0	6.1	0.0	0.129447	102036	104255	2748-2915	1.000000	84.7
ndhF_p4	ndhF	4	GGGGATAGGAAAGGGGAAGA	GGTATGGAAGCCTATCCCCT	20	20	55.0	55.0	57.8	58.0	0.0	0.0	0.254206	102036	104255	2690-2864	1.000000	83.7
ndhF_p5	ndhF	5	GCATGGTGTTCTTCGACTGA	GGTATGGAAGCCTATCCCCT	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.250512	102036	104255	2843-2999	0.666667	69.2
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCT	20	24	55.0	29.2	58.1	55.3	0.0	0.0	6.833076	104256	105084	777-979	1.000000	46.4
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCTT	20	25	55.0	28.0	58.1	56.0	0.0	0.0	7.158227	104256	105084	777-979	1.000000	46.4
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAGTGGGAGAAGAGGGAT	TTCCAAATATTTTTACGAATACGCT	20	25	55.0	28.0	58.1	56.0	0.0	0.0	7.158227	104256	105084	778-980	1.000000	46.4
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TCTTCCAAATATTTTTACGAATACGC	20	26	55.0	30.8	58.1	56.9	0.0	0.0	7.164867	104256	105084	780-982	1.000000	46.4
ndhF-rpl32_p5	ndhF-rpl32	5	AAGGCCAAATCCCATTGACA	TCCAAATATTTTTACGAATACGCT	20	24	45.0	29.2	57.7	55.3	5.0	0.0	7.043497	104256	105084	805-1007	1.000000	46.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAACAACAACAAACAAA	TTCGAACCGAGATGCTTGAG	20	20	40.0	50.0	56.1	58.0	0.0	0.0	1.936305	105274	105904	727-737	1.000000	67.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAACAACAACAAACAAA	GGATTCGAACCGAGATGCTT	20	20	40.0	50.0	56.1	58.1	0.0	0.0	1.991272	105274	105904	730-740	1.000000	66.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGCAACAACAACAAACAAA	TCCTAAGAGCAGCGTGTCTA	20	20	40.0	50.0	56.1	57.9	0.0	0.0	2.056874	105274	105904	700-710	1.000000	66.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGCAACAACAACAAACAAA	AGCAGCGTGTCTACCAATTT	20	20	40.0	45.0	56.1	57.8	0.0	0.0	2.117601	105274	105904	693-703	1.000000	65.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAACAACAACAAACAAA	GCTTGAGCACTGCTTCCTAA	20	20	40.0	50.0	56.1	58.2	0.0	4.7	2.119083	105274	105904	714-724	1.000000	65.8
ndhG-ndhI_p1	ndhG-ndhI	1	AAGTTGTGCCACAGCTACAA	GAGTACTGTCCGACAAGCTG	20	20	45.0	55.0	57.9	58.0	14.8	0.0	0.067362	110645	110898	644-653	1.000000	82.1
ndhG-ndhI_p2	ndhG-ndhI	2	AGTTGTGCCACAGCTACAAA	GAGTACTGTCCGACAAGCTG	20	20	45.0	55.0	57.9	58.0	12.8	0.0	0.067362	110645	110898	643-652	1.000000	82.1
ndhG-ndhI_p3	ndhG-ndhI	3	TTGTGCCACAGCTACAAAGT	GAGTACTGTCCGACAAGCTG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.067362	110645	110898	641-650	1.000000	82.1
ndhG-ndhI_p4	ndhG-ndhI	4	AGAAGTTGTGCCACAGCTAC	GAGTACTGTCCGACAAGCTG	20	20	50.0	55.0	58.1	58.0	14.8	0.0	0.131792	110645	110898	646-655	1.000000	81.5
ndhG-ndhI_p5	ndhG-ndhI	5	TTGTGCCACAGCTACAAAGT	GTGTTCGCGTATGTCCGATA	20	20	45.0	50.0	57.9	57.9	0.0	5.8	0.160943	110645	110898	766-775	1.000000	81.0