primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AGAGCACCCTCATTCCGATA	TCGGAGGTTCGAATCCTTTC	20	20	50.0	50.0	57.9	57.3	0.0	16.9	0.755578	6216	7984	1817-1913	1.000000	78.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AGAGCACCCTCATTCCGATA	TAAGGCAACGGGTTTTGGTC	20	20	50.0	50.0	57.9	58.7	0.0	0.0	0.767598	6216	7984	1844-1940	1.000000	78.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TCGGAGGTTCGAATCCTTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6216	7984	1749-1845	1.000000	77.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6216	7984	1776-1872	1.000000	77.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AGAGCACCCTCATTCCGATA	GGGTTTTGGTCCCGCTATTC	20	20	50.0	55.0	57.9	58.9	0.0	0.0	0.987595	6216	7984	1835-1931	1.000000	76.3
atpH-atpI_p1	atpH-atpI	1	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	57.9	58.5	0.0	0.0	0.666168	14760	15931	1271-1276	1.000000	76.4
atpH-atpI_p2	atpH-atpI	2	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.8	58.5	0.0	0.0	0.687386	14760	15931	1263-1268	1.000000	76.2
atpH-atpI_p3	atpH-atpI	3	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.8	58.5	0.0	0.0	0.732109	14760	15931	1270-1275	1.000000	75.8
atpH-atpI_p4	atpH-atpI	4	AGCCAATCCAGCAGCAATAA	CCGCGGCTTATATAGGTGAA	20	20	45.0	50.0	57.9	57.3	0.0	14.7	0.884589	14760	15931	1256-1261	1.000000	74.6
atpH-atpI_p5	atpH-atpI	5	CAGCAGCAATAACAGAAGCG	CCGCGGCTTATATAGGTGAA	20	20	50.0	50.0	57.8	57.3	0.0	14.7	0.905807	14760	15931	1248-1253	1.000000	74.5
rps2-rpoC2_p1	rps2-rpoC2	1	AACTCCTGCCTCCATCATCT	ATTAGTGCACCCTTCAAGGC	20	20	50.0	50.0	58.1	58.2	0.0	14.6	0.270024	17596	17897	462-462	1.000000	79.1
rps2-rpoC2_p2	rps2-rpoC2	2	TGCCTCCATCATCTCTTCCA	ATTAGTGCACCCTTCAAGGC	20	20	50.0	50.0	58.1	58.2	0.0	14.6	0.276899	17596	17897	456-456	1.000000	79.0
rps2-rpoC2_p3	rps2-rpoC2	3	AACTCCTGCCTCCATCATCT	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.357147	17596	17897	401-401	1.000000	78.5
rps2-rpoC2_p4	rps2-rpoC2	4	TGCCTCCATCATCTCTTCCA	TTCGAGGGGGAAATGAGAGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.364021	17596	17897	395-395	1.000000	78.5
rps2-rpoC2_p5	rps2-rpoC2	5	TCCTGCCTCCATCATCTCTT	ATTAGTGCACCCTTCAAGGC	20	20	50.0	50.0	57.8	58.2	0.0	14.6	0.347815	17596	17897	459-459	1.000000	78.4
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	CTAGTCCCTACCGCCTTTCT	20	20	50.0	55.0	58.2	58.2	0.0	0.0	0.373408	30571	31775	1302-1348	1.000000	80.2
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TCTAGTCCCTACCGCCTTTC	20	20	50.0	55.0	58.2	58.2	0.0	0.0	0.378512	30571	31775	1303-1349	1.000000	80.2
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TCCCTACCGCCTTTCTACTT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.394230	30571	31775	1298-1344	1.000000	80.1
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TTCTAGTCCCTACCGCCTTT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.394230	30571	31775	1304-1350	1.000000	80.0
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	CATTCTAGTCCCTACCGCCT	20	20	50.0	55.0	58.2	58.3	0.0	0.0	0.445475	30571	31775	1306-1352	1.000000	79.6
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	33441	34267	904-914	1.000000	82.6
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	33441	34267	902-912	1.000000	82.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	33441	34267	883-893	1.000000	82.2
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	33441	34267	881-891	1.000000	82.2
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AACTTCCTTTCGCAGTACCC	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.186403	33441	34267	965-975	1.000000	82.1
rbcL-accD_p1	rbcL-accD	1	GCGTTGGAGAGATCGTTTCT	TGCTTGTATGAGGGAGTGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051122	59286	60186	2416-2998	1.000000	84.1
rbcL-accD_p2	rbcL-accD	2	GCACGACTACTTAACAGGGG	TGCTTGTATGAGGGAGTGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.054761	59286	60186	2254-2836	1.000000	84.1
rbcL-accD_p3	rbcL-accD	3	AATGAGGGACGTGATCTTGC	TGCTTGTATGAGGGAGTGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.078629	59286	60186	1758-2340	1.000000	84.0
rbcL-accD_p4	rbcL-accD	4	ATGAAATTATCCGTGCGGCT	TGCTTGTATGAGGGAGTGGA	20	20	45.0	50.0	58.0	58.0	5.6	0.0	0.081472	59286	60186	1727-2309	1.000000	84.0
rbcL-accD_p5	rbcL-accD	5	GGTAGCTGCCGAATCTTCAA	TCTCATCGCAACTCCGAATG	20	20	50.0	50.0	58.0	58.1	4.6	0.0	0.097614	59286	60186	2529-2610	1.000000	83.5
accD-psaI_p1	accD-psaI	1	TCAGGTCCATGGTTTCTTTCC	CCATTGCAATTGCCGGAAAT	21	20	47.6	45.0	57.8	58.0	12.6	26.9	1.181831	62304	62875	693-833	1.000000	75.2
accD-psaI_p2	accD-psaI	2	CAGGTCCATGGTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	21	20	47.6	45.0	57.8	58.0	12.6	26.9	1.187682	62304	62875	692-832	1.000000	75.1
accD-psaI_p3	accD-psaI	3	TCAGGTCCATGGTTTCTTTCC	AAGAAGCCATTGCAATTGCC	21	20	47.6	45.0	57.8	57.9	12.6	26.8	1.258364	62304	62875	699-839	1.000000	74.6
accD-psaI_p4	accD-psaI	4	CAGGTCCATGGTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	21	20	47.6	45.0	57.8	57.9	12.6	26.8	1.264216	62304	62875	698-838	1.000000	74.5
accD-psaI_p5	accD-psaI	5	TCAGGTCCATGGTTTCTTTCC	AGGCACAAAAACAGAGGGAA	21	20	47.6	45.0	57.8	57.5	12.6	0.0	1.607428	62304	62875	659-799	1.000000	71.8
psaJ-rpl33_p1	psaJ-rpl33	1	TGGCACCGGTACTAAGTACT	GAAATGCCCGTTGATACCCT	20	20	50.0	50.0	57.8	57.9	6.9	0.0	0.273435	71467	71963	670-696	1.000000	81.2
psaJ-rpl33_p2	psaJ-rpl33	2	GTTCGGGTCTTTAGCAGGTC	GAAATGCCCGTTGATACCCT	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.329563	71467	71963	645-671	1.000000	80.9
psaJ-rpl33_p3	psaJ-rpl33	3	GTGGCACCGGTACTAAGTAC	GAAATGCCCGTTGATACCCT	20	20	55.0	50.0	57.7	57.9	6.9	0.0	0.337045	71467	71963	671-697	1.000000	80.7
psaJ-rpl33_p4	psaJ-rpl33	4	TGGCACCGGTACTAAGTACT	CGTTTCGGACACAACTGGTA	20	20	50.0	50.0	57.8	58.1	6.9	0.0	0.365139	71467	71963	642-668	1.000000	80.6
psaJ-rpl33_p5	psaJ-rpl33	5	TTCGGGTCTTTAGCAGGTCT	GAAATGCCCGTTGATACCCT	20	20	50.0	50.0	58.4	57.9	0.0	0.0	0.411866	71467	71963	644-670	1.000000	80.2
rpl22_p1	rpl22	1	GTTGTGCAAACCAAAGGGAA	TCGCGTCGTTAAGAAATGTT	20	20	45.0	40.0	57.3	56.1	0.0	5.8	2.557779	87144	87746	632-764	1.000000	64.3
rpl22_p2	rpl22	2	GTTGTGCAAACCAAAGGGAA	GATCGCGTCGTTAAGAAATGT	20	21	45.0	42.9	57.3	57.1	0.0	5.8	2.583823	87144	87746	634-766	1.000000	64.1
rpl22_p3	rpl22	3	TGTGCAAACCAAAGGGAATG	TCGCGTCGTTAAGAAATGTT	20	20	45.0	40.0	57.1	56.1	0.0	5.8	2.782315	87144	87746	630-762	1.000000	62.5
rpl22_p4	rpl22	4	GTTGTGCAAACCAAAGGGAA	ATCGCGTCGTTAAGAAATGT	20	20	45.0	40.0	57.3	55.9	0.0	5.8	2.783684	87144	87746	633-765	1.000000	62.5
rpl22_p5	rpl22	5	TGTGCAAACCAAAGGGAATG	GATCGCGTCGTTAAGAAATGT	20	21	45.0	42.9	57.1	57.1	0.0	5.8	2.808359	87144	87746	632-764	1.000000	62.3
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	TGGCTTTTCCCGGATGAAAA	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	58.0	58.0	15.2	15.9	0.010336	97700	98323	719-765	1.000000	91.9
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	TGGCTTTTCCCGGATGAAAA	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	58.0	58.0	15.2	15.9	0.011706	97700	98323	721-767	1.000000	91.9
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	TGGCTTTTCCCGGATGAAAA	CCAACACTCCACCTTTGTCA	20	20	45.0	50.0	58.0	57.9	15.2	0.0	0.053840	97700	98323	813-859	1.000000	91.6
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	CAAGAGGGTGGTGGAGAGTA	AAATGGTAGACACGCGAGAC	20	20	55.0	50.0	58.1	58.0	0.0	15.9	0.069246	97700	98323	1202-1383	1.000000	90.1
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	CAAGAGGGTGGTGGAGAGTA	TGAAATGGTAGACACGCGAG	20	20	55.0	50.0	58.1	58.0	0.0	15.9	0.070616	97700	98323	1204-1385	1.000000	90.0
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CCCCGTAACTCTTCTTCAGC	TAGGAACTGGGAGTGGAAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.058919	113321	114690	1843-2092	1.000000	91.5
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	CCCGTAACTCTTCTTCAGCC	TAGGAACTGGGAGTGGAAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.058919	113321	114690	1842-2091	1.000000	91.5
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	CCCCGTAACTCTTCTTCAGC	GCGCCGGCAAATAATAGAAC	20	20	55.0	50.0	58.0	58.0	0.0	29.0	0.043954	113321	114690	2495-2495	0.500000	59.6
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	CCCGTAACTCTTCTTCAGCC	GCGCCGGCAAATAATAGAAC	20	20	55.0	50.0	58.0	58.0	0.0	29.0	0.043954	113321	114690	2494-2494	0.500000	59.6
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GCCCCGTAACTCTTCTTCAG	GCGCCGGCAAATAATAGAAC	20	20	55.0	50.0	58.0	58.0	0.0	29.0	0.043954	113321	114690	2496-2496	0.500000	59.6
ycf1_p1	ycf1	1	GGAGAAGCAGGTTCGCAATA	TGCCGAAGTGATGGAAAACA	20	20	50.0	45.0	58.0	58.0	16.6	0.0	0.049911	131414	131663	925-1132	1.000000	90.0
ycf1_p2	ycf1	2	CAGGTTTTCCCAGGCTTCTT	TGCCGAAGTGATGGAAAACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.026811	131414	131663	1146-1376	1.000000	89.0
ycf1_p3	ycf1	3	CAGGTTTTCCCAGGCTTCTT	CATATCCGCCCAGTTTGTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038033	131414	131663	1112-1342	1.000000	89.0
ycf1_p4	ycf1	4	CAGGTTTTCCCAGGCTTCTT	ACATATCCGCCCAGTTTGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039463	131414	131663	1113-1343	1.000000	89.0
ycf1_p5	ycf1	5	CAGGTTTTCCCAGGCTTCTT	AGTCAAGAATGGAATCGCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045199	131414	131663	1239-1469	1.000000	88.5
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAA	20	20	50.0	45.0	58.0	58.0	15.9	15.2	0.010336	149477	150099	719-765	1.000000	91.9
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAA	20	20	50.0	45.0	58.0	58.0	15.9	15.2	0.011706	149477	150099	721-767	1.000000	91.9
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	CCAACACTCCACCTTTGTCA	TGGCTTTTCCCGGATGAAAA	20	20	50.0	45.0	57.9	58.0	0.0	15.2	0.053840	149477	150099	813-859	1.000000	91.6
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	AAATGGTAGACACGCGAGAC	CAAGAGGGTGGTGGAGAGTA	20	20	50.0	55.0	58.0	58.1	15.9	0.0	0.069246	149477	150099	1202-1383	1.000000	90.1
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	TGAAATGGTAGACACGCGAG	CAAGAGGGTGGTGGAGAGTA	20	20	50.0	55.0	58.0	58.1	15.9	0.0	0.070616	149477	150099	1204-1385	1.000000	90.0
ycf2_p1	ycf2	1	AAATGGTAGACACGCGAGAC	GAACCCTGGAGATCCACTCT	20	20	50.0	55.0	58.0	58.1	15.9	0.0	0.135058	150100	150219	1053-1202	1.000000	90.5
ycf2_p2	ycf2	2	TGAAATGGTAGACACGCGAG	GAACCCTGGAGATCCACTCT	20	20	50.0	55.0	58.0	58.1	15.9	0.0	0.136428	150100	150219	1055-1204	1.000000	90.5
ycf2_p3	ycf2	3	AAATGGTAGACACGCGAGAC	CAAGAGGGTGGTGGAGAGTA	20	20	50.0	55.0	58.0	58.1	15.9	0.0	0.069246	150100	150219	1202-1383	1.000000	90.1
ycf2_p4	ycf2	4	TGAAATGGTAGACACGCGAG	CAAGAGGGTGGTGGAGAGTA	20	20	50.0	55.0	58.0	58.1	15.9	0.0	0.070616	150100	150219	1204-1385	1.000000	90.0
ycf2_p5	ycf2	5	GCCTTGGTGGTGAAATGGTA	CAAGAGGGTGGTGGAGAGTA	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.143543	150100	150219	1214-1395	1.000000	89.4