primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	rps16-trnQ	1	AAGTCGCACGTTGCTTTCTA	TAAAACCTGAAATGGGGCGT	20	20	45.0	45.0	58.1	57.7	8.1	0.0	0.435397	5531	6634	1237-1250	1.000000	77.3
rps16-trnQ_p2	rps16-trnQ	2	TCATGTCCTTCAAGTCGCAC	TAAAACCTGAAATGGGGCGT	20	20	50.0	45.0	58.2	57.7	0.0	0.0	0.500305	5531	6634	1248-1261	1.000000	76.8
rps16-trnQ_p3	rps16-trnQ	3	AAGTCGCACGTTGCTTTCTA	CGTTACTCGGAGGTTCGAAC	20	20	45.0	55.0	58.1	58.4	8.1	20.1	0.528281	5531	6634	1186-1199	1.000000	76.6
rps16-trnQ_p4	rps16-trnQ	4	TCATGTCCTTCAAGTCGCAC	CGTTACTCGGAGGTTCGAAC	20	20	50.0	55.0	58.2	58.4	0.0	20.1	0.593190	5531	6634	1197-1210	1.000000	76.1
rps16-trnQ_p5	rps16-trnQ	5	AAGTCGCACGTTGCTTTCTA	TAAGGCAGCGGGTTTTGATC	20	20	45.0	50.0	58.1	58.5	8.1	0.0	0.679874	5531	6634	1207-1220	1.000000	75.4
trnQ-psbI_p1	trnQ-psbI	1	GGTTCGAACCTCCGAGTAAC	CCTGGGTCATTGGATAAGAA	20	20	55.0	45.0	58.0	54.4	35.0	0.0	3.621745	6707	7636	1076-1089	1.000000	51.9
trnQ-psbI_p2	trnQ-psbI	2	GGTTCGAACCTCCGAGTAAC	CCTGGGTCATTGGATAAGAATC	20	22	55.0	45.5	58.0	56.3	35.0	0.0	3.741913	6707	7636	1076-1089	1.000000	51.0
trnQ-psbI_p3	trnQ-psbI	3	GGTTCGAACCTCCGAGTAAC	CCTGGGTCATTGGATAAGAAT	20	21	55.0	42.9	58.0	55.0	35.0	0.0	3.991139	6707	7636	1076-1089	1.000000	49.0
trnQ-psbI_p4	trnQ-psbI	4	GTTCGAACCTCCGAGTAACG	CCTGGGTCATTGGATAAGAA	20	20	55.0	45.0	58.4	54.4	17.7	0.0	3.998766	6707	7636	1075-1088	1.000000	48.9
trnQ-psbI_p5	trnQ-psbI	5	GTTCGAACCTCCGAGTAACG	CCTGGGTCATTGGATAAGAATC	20	22	55.0	45.5	58.4	56.3	17.7	0.0	4.118934	6707	7636	1075-1088	1.000000	47.9
trnS-psbD_p1	trnS-psbD	1	ATTAGCAATCCGCCGCTTTA	GACAAAAACGAAACGGTCCC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.147815	7957	8943	1120-1136	1.000000	79.9
trnS-psbD_p2	trnS-psbD	2	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	20	20	45.0	55.0	58.0	58.1	0.0	5.0	0.148662	7957	8943	1141-1157	1.000000	79.9
trnS-psbD_p3	trnS-psbD	3	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.284276	7957	8943	1119-1135	1.000000	78.8
trnS-psbD_p4	trnS-psbD	4	ATTAGCAATCCGCCGCTTTA	CCGACAAAAACGAAACGGTC	20	20	45.0	50.0	58.0	58.3	0.0	0.0	0.304501	7957	8943	1122-1138	1.000000	78.6
trnS-psbD_p5	trnS-psbD	5	ATTAGCAATCCGCCGCTTTA	CGACAAAAACGAAACGGTCC	20	20	45.0	50.0	58.0	58.3	0.0	0.0	0.304501	7957	8943	1121-1137	1.000000	78.6
trnG-trnT_p1	trnG-trnT	1	ACGAATCACACTTTTACCACT	ACCATGGCATTACTCTAGCAC	21	21	38.1	47.6	55.5	57.8	0.0	13.0	4.735723	13728	15024	1431-1434	1.000000	43.0
trnG-trnT_p2	trnG-trnT	2	ACGAATCACACTTTTACCACT	GACTTACGTCTTACCATGGCA	21	21	38.1	47.6	55.5	57.8	0.0	13.0	4.776909	13728	15024	1443-1446	1.000000	42.6
trnG-trnT_p3	trnG-trnT	3	ACGAATCACACTTTTACCACT	TGACTTACGTCTTACCATGGC	21	21	38.1	47.6	55.5	57.8	0.0	13.0	4.776909	13728	15024	1444-1447	1.000000	42.6
trnG-trnT_p4	trnG-trnT	4	AGAACGAATCACACTTTTACCAC	ACCATGGCATTACTCTAGCAC	23	21	39.1	47.6	57.3	57.8	0.0	13.0	4.868438	13728	15024	1434-1437	1.000000	41.9
trnG-trnT_p5	trnG-trnT	5	GAACGAATCACACTTTTACCACT	ACCATGGCATTACTCTAGCAC	23	21	39.1	47.6	57.3	57.8	0.0	13.0	4.868438	13728	15024	1433-1436	1.000000	41.9
atpI-atpH_p1	atpI-atpH	1	TTTTGCAACATTAGCCGCAG	GCAATAACAGAAGCAGCAGC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.157706	31408	32014	646-697	1.000000	83.0
atpI-atpH_p2	atpI-atpH	2	TTTTGCAACATTAGCCGCAG	GCAGCAATAACAGAAGCAGC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.157706	31408	32014	649-700	1.000000	83.0
atpI-atpH_p3	atpI-atpH	3	TTTTGCAACATTAGCCGCAG	AGCCAATCCAGCAGCAATAA	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.211601	31408	32014	659-710	1.000000	82.5
atpI-atpH_p4	atpI-atpH	4	TTTTGCAACATTAGCCGCAG	GCAGTACCTTGACCAACTCC	20	20	45.0	55.0	57.9	58.2	0.0	0.0	0.253472	31408	32014	703-754	1.000000	82.2
atpI-atpH_p5	atpI-atpH	5	TTTTGCAACATTAGCCGCAG	AAGCAGCAGCAATTAGTGGA	20	20	45.0	45.0	57.9	57.8	0.0	3.2	0.268178	31408	32014	636-687	1.000000	82.1
trnR-rps14_p1	trnR-rps14	1	AGAAGACCTCTGTCCTATCCA	TATCCGGACACGTACTTCGA	21	20	47.6	50.0	56.9	58.0	0.0	6.1	2.074369	35710	36096	471-494	1.000000	67.9
trnR-rps14_p2	trnR-rps14	2	AGGTTTAGAAGACCTCTGTCCT	TATCCGGACACGTACTTCGA	22	20	45.5	50.0	57.9	58.0	0.0	6.1	2.157697	35710	36096	477-500	1.000000	67.2
trnR-rps14_p3	trnR-rps14	3	AGAAGACCTCTGTCCTATCCA	GCTATCCGGACACGTACTTC	21	20	47.6	55.0	56.9	57.9	0.0	6.1	2.168392	35710	36096	473-496	1.000000	67.1
trnR-rps14_p4	trnR-rps14	4	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	21	20	47.6	50.0	56.9	58.2	0.0	10.7	2.235530	35710	36096	430-453	1.000000	66.8
trnR-rps14_p5	trnR-rps14	5	AGGTTTAGAAGACCTCTGTCCT	GCTATCCGGACACGTACTTC	22	20	45.5	55.0	57.9	57.9	0.0	6.1	2.251720	35710	36096	479-502	1.000000	66.4
psaA-ycf3_p1	psaA-ycf3	1	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.1	58.0	0.0	18.7	0.091171	41042	41638	758-764	1.000000	79.7
psaA-ycf3_p2	psaA-ycf3	2	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.132613	41042	41638	754-760	1.000000	79.4
psaA-ycf3_p3	psaA-ycf3	3	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	57.8	58.0	0.0	18.7	0.235180	41042	41638	756-762	1.000000	78.5
psaA-ycf3_p4	psaA-ycf3	4	TCTGGCCCATTCCTCAAAAG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	57.8	58.0	0.0	18.7	0.235180	41042	41638	757-763	1.000000	78.5
psaA-ycf3_p5	psaA-ycf3	5	CTGGCCCATTCCTCAAAAGA	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.276621	41042	41638	752-758	1.000000	78.2
ycf3-trnS_p1	ycf3-trnS	1	TTTTTCCCCTGAGGTTGTCG	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.175689	43622	44220	786-792	1.000000	79.2
ycf3-trnS_p2	ycf3-trnS	2	TTTTTCCCCTGAGGTTGTCG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.0	57.8	0.0	5.6	0.217004	43622	44220	736-742	1.000000	78.9
ycf3-trnS_p3	ycf3-trnS	3	CATTTCCACGGGATCTAGGC	ACGGAAAGAGAGGGATTCGA	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.267113	43622	44220	709-715	1.000000	78.5
ycf3-trnS_p4	ycf3-trnS	4	CATTTCCACGGGATCTAGGC	TTCCAATGCTACGCCTTGAA	20	20	55.0	45.0	58.1	57.8	0.0	5.6	0.308428	43622	44220	659-665	1.000000	78.3
ycf3-trnS_p5	ycf3-trnS	5	TTTTTCCCCTGAGGTTGTCG	AGTTCCAATGCTACGCCTTG	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.571186	43622	44220	738-744	1.000000	76.1
trnL_p1	trnL	1	ACAGGGAATCCAGGTCTCAA	GTTCTCTTTGCACTCTTTTGTGA	20	23	50.0	39.1	58.0	57.8	0.1	0.0	3.280098	46060	46687	777-777	1.000000	54.2
trnL_p2	trnL	2	ACAGGGAATCCAGGTCTCAA	AGTTCTCTTTGCACTCTTTTGTG	20	23	50.0	39.1	58.0	57.8	0.1	0.0	3.285505	46060	46687	778-778	1.000000	54.1
trnL_p3	trnL	3	ACAGGGAATCCAGGTCTCAA	TCTCTTTGCACTCTTTTGTGA	20	21	50.0	38.1	58.0	55.7	0.1	0.0	3.377399	46060	46687	775-775	1.000000	53.4
trnL_p4	trnL	4	ACAGGGAATCCAGGTCTCAA	ACAGGTTAGGGAATAAAAGAGGG	20	23	50.0	43.5	58.0	57.5	0.1	0.0	3.497134	46060	46687	686-686	1.000000	52.5
trnL_p5	trnL	5	ACAGGGAATCCAGGTCTCAA	CAGGTTAGGGAATAAAAGAGGGT	20	23	50.0	43.5	58.0	57.5	0.1	0.0	3.497134	46060	46687	685-685	1.000000	52.5
ndhC-trnV_p1	ndhC-trnV	1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.0	57.9	6.3	0.0	0.110083	49214	50096	972-1009	1.000000	82.7
ndhC-trnV_p2	ndhC-trnV	2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	20	20	55.0	55.0	58.0	58.3	6.3	22.8	0.339465	49214	50096	973-1010	1.000000	80.8
ndhC-trnV_p3	ndhC-trnV	3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.3	57.9	6.3	0.0	0.415737	49214	50096	971-1008	1.000000	80.2
ndhC-trnV_p4	ndhC-trnV	4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.5	57.9	6.3	0.0	0.560721	49214	50096	974-1011	1.000000	79.1
ndhC-trnV_p5	ndhC-trnV	5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	58.3	58.3	6.3	22.8	0.645119	49214	50096	972-1009	1.000000	78.4
rbcL-psaI_p1	rbcL-psaI	1	TCGAGTTCGAGCCAGTAGAT	AGAAGGTAAGTTGAAATCCGTCA	20	23	50.0	39.1	58.0	57.8	0.0	0.0	3.263267	55285	56249	1024-1030	1.000000	54.8
rbcL-psaI_p2	rbcL-psaI	2	CGCAGCTTGTGAAGTATGGA	AGAAGGTAAGTTGAAATCCGTCA	20	23	50.0	39.1	58.3	57.8	0.0	0.0	3.497808	55285	56249	1056-1062	1.000000	52.9
rbcL-psaI_p3	rbcL-psaI	3	GGCGATCAAATTCGAGTTCG	AGAAGGTAAGTTGAAATCCGTCA	20	23	50.0	39.1	57.6	57.8	0.0	0.0	3.576707	55285	56249	1035-1041	1.000000	52.3
rbcL-psaI_p4	rbcL-psaI	4	TGGAAGGCGATCAAATTCGA	AGAAGGTAAGTTGAAATCCGTCA	20	23	45.0	39.1	57.6	57.8	0.0	0.0	3.618379	55285	56249	1040-1046	1.000000	52.0
rbcL-psaI_p5	rbcL-psaI	5	TCGAGTTCGAGCCAGTAGAT	GAAGGTAAGTTGAAATCCGTCA	20	22	50.0	40.9	58.0	56.4	0.0	0.0	3.657289	55285	56249	1023-1029	1.000000	51.7
psbL-psbF_p1	psbL-psbF	1	CATTCGGGTTTGATTGTGTCA	CTGGCTATTCACGGACTAGC	21	20	42.9	55.0	57.1	57.9	0.0	12.0	2.055083	60523	60544	122-122	1.000000	64.2
psbL-psbF_p2	psbL-psbF	2	CATTCGGGTTTGATTGTGTCA	GCTGGCTATTCACGGACTAG	21	20	42.9	55.0	57.1	57.9	0.0	0.0	2.055083	60523	60544	123-123	1.000000	64.2
psbL-psbF_p3	psbL-psbF	3	CATTCGGGTTTGATTGTGTCA	GGCTATTCACGGACTAGCTG	21	20	42.9	55.0	57.1	57.9	0.0	16.8	2.055083	60523	60544	120-120	1.000000	64.2
psbL-psbF_p4	psbL-psbF	4	CATTCGGGTTTGATTGTGTCA	TTCACGGACTAGCTGTACCT	21	20	42.9	50.0	57.1	57.8	0.0	0.0	2.124798	60523	60544	115-115	1.000000	63.7
psbL-psbF_p5	psbL-psbF	5	CATTCGGGTTTGATTGTGTCA	TGGCTATTCACGGACTAGCT	21	20	42.9	50.0	57.1	58.2	0.0	16.8	2.136886	60523	60544	121-121	1.000000	63.6
rpl16_p1	rpl16	1	GAACGAAAAACCTGGCGTTT	TTCCCGAAATCCCTGAAAAA	20	20	45.0	40.0	57.8	55.1	9.8	0.0	3.065965	76423	77857	1557-1572	1.000000	56.6
rpl16_p2	rpl16	2	GAACGAAAAACCTGGCGTTT	TTTCCCGAAATCCCTGAAAA	20	20	45.0	40.0	57.8	55.1	9.8	0.0	3.065965	76423	77857	1558-1573	1.000000	56.6
rpl16_p3	rpl16	3	GAACGAAAAACCTGGCGTTT	TTTTCCCGAAATCCCTGAAA	20	20	45.0	40.0	57.8	55.1	9.8	0.0	3.065965	76423	77857	1559-1574	1.000000	56.6
rpl16_p4	rpl16	4	GAACGAAAAACCTGGCGTTT	TTTTTCCCGAAATCCCTGAA	20	20	45.0	40.0	57.8	55.1	9.8	0.0	3.065965	76423	77857	1560-1575	1.000000	56.6
rpl16_p5	rpl16	5	GAACGAAAAACCTGGCGTTT	TTTCCCGAAATCCCTGAAAAA	20	21	45.0	38.1	57.8	56.0	9.8	0.0	3.243476	76423	77857	1558-1573	1.000000	55.2
trnH-rpl2_p1	trnH-rpl2	1	CTTTAGCTGGATAAGGGGCG	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.0	22.4	0.139940	79788	79842	223-223	1.000000	79.2
trnH-rpl2_p2	trnH-rpl2	2	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.2	22.4	0.153095	79788	79842	205-205	1.000000	79.2
trnH-rpl2_p3	trnH-rpl2	3	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.157457	79788	79842	191-191	1.000000	79.2
trnH-rpl2_p4	trnH-rpl2	4	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	79788	79842	190-190	1.000000	79.1
trnH-rpl2_p5	trnH-rpl2	5	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	79788	79842	192-192	1.000000	79.1
ndhF-trnL_p1	ndhF-trnL	1	GGAAGTGGGAGAAGAGGGAT	TTCGAACCGAGATGCTTGAG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.117286	102224	103692	1581-1585	1.000000	80.9
ndhF-trnL_p2	ndhF-trnL	2	GGAAGTGGGAGAAGAGGGAT	GGATTCGAACCGAGATGCTT	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.172253	102224	103692	1584-1588	1.000000	80.4
ndhF-trnL_p3	ndhF-trnL	3	GGAAGTGGGAGAAGAGGGAT	TCCTAAGAGCAGCGTGTCTA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.237855	102224	103692	1554-1558	1.000000	79.9
ndhF-trnL_p4	ndhF-trnL	4	GGAAGTGGGAGAAGAGGGAT	AGCAGCGTGTCTACCAATTT	20	20	55.0	45.0	58.1	57.8	0.0	0.0	0.298582	102224	103692	1547-1551	1.000000	79.5
ndhF-trnL_p5	ndhF-trnL	5	GGAAGTGGGAGAAGAGGGAT	GCTTGAGCACTGCTTCCTAA	20	20	55.0	50.0	58.1	58.2	0.0	4.7	0.300064	102224	103692	1568-1572	1.000000	79.4
psaC-ndhE_p1	psaC-ndhE	1	GCTTTACATCCATCCCAGGG	AGCCGCTATTGGACTATCCA	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.296086	106855	107350	691-700	1.000000	78.8
psaC-ndhE_p2	psaC-ndhE	2	GGCTTTACATCCATCCCAGG	AGCCGCTATTGGACTATCCA	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.296086	106855	107350	692-701	1.000000	78.8
psaC-ndhE_p3	psaC-ndhE	3	GTTGGACAAGCTCGTACACA	AGCCGCTATTGGACTATCCA	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.422292	106855	107350	652-661	1.000000	77.8
psaC-ndhE_p4	psaC-ndhE	4	TGTTGGACAAGCTCGTACAC	AGCCGCTATTGGACTATCCA	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.422292	106855	107350	653-662	1.000000	77.8
psaC-ndhE_p5	psaC-ndhE	5	CTGTTGGACAAGCTCGTACA	AGCCGCTATTGGACTATCCA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.431884	106855	107350	654-663	1.000000	77.8