primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTAGCTGGTGTTGAAGCTCC	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.313924	65	318	311-323	1.000000	79.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	CTGGTGTTGAAGCTCCTTCT	20	20	55.0	50.0	58.1	57.4	0.0	6.5	0.686839	65	318	307-319	1.000000	76.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CGCATGGTGGATTCACAATC	TTAGCTGGTGTTGAAGCTCC	20	20	50.0	50.0	57.5	57.8	6.4	0.0	0.665228	65	318	340-352	1.000000	76.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATTCACAATCCACCGCCTT	TTAGCTGGTGTTGAAGCTCC	20	20	50.0	50.0	58.5	57.8	0.0	0.0	0.721330	65	318	331-343	1.000000	76.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TAGCTGGTGTTGAAGCTCCT	20	20	55.0	50.0	58.1	58.6	0.0	0.0	0.776837	65	318	310-322	1.000000	75.8
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	TGGTCCTGTTATTCGGAGGT	20	20	45.0	50.0	58.1	58.1	8.1	0.0	0.192340	6025	7092	1156-1157	1.000000	83.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6025	7092	1141-1142	1.000000	83.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6025	7092	1145-1146	1.000000	78.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACCGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	19.6	0.818817	6025	7092	1171-1172	1.000000	78.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	TGGTCCTGTTATTCGGAGGT	20	20	50.0	50.0	57.2	58.1	0.0	0.0	0.840738	6025	7092	1148-1149	1.000000	77.9
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	GTAATCCTGGGCGTGATGAA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.330166	8482	8593	203-208	1.000000	81.6
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	GTAATCCTGGGCGTGATGAA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.331554	8482	8593	204-209	1.000000	81.6
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	GTAATCCTGGGCGTGATGAA	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	57.7	58.2	0.0	17.2	0.542512	8482	8593	153-158	1.000000	81.3
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	GTAATCCTGGGCGTGATGAA	TTCGAATCCCTCTCTCTCCG	20	20	50.0	55.0	57.7	58.3	0.0	0.0	0.644045	8482	8593	150-155	1.000000	80.6
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	GTAATCCTGGGCGTGATGAA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.435031	8482	8593	222-227	1.000000	80.4
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.0	58.5	1.4	0.0	0.556109	14638	15454	864-905	1.000000	80.9
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.9	58.5	0.0	0.0	0.630002	14638	15454	920-961	1.000000	80.2
atpH-atpI_p3	atpH-atpI	3	AACGGAAGCGGCAGAAATAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.2	58.5	1.4	0.0	0.701648	14638	15454	862-903	1.000000	79.7
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.2	58.5	1.4	0.0	0.701648	14638	15454	865-906	1.000000	79.7
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	TTTTTGCAACTTTAGCCGCG	20	20	55.0	45.0	58.2	58.5	0.0	0.0	0.708039	14638	15454	924-965	1.000000	79.6
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	32354	32884	618-619	1.000000	80.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	32354	32884	616-617	1.000000	80.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	32354	32884	597-598	1.000000	80.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	32354	32884	595-596	1.000000	80.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.217323	32354	32884	615-616	1.000000	80.3
pafI-trnS-UCC_p1	pafI-trnS-UCC	1	TCTCCTGAGGTTGTGGGAAT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.139155	45493	45960	628-631	1.000000	80.7
pafI-trnS-UCC_p2	pafI-trnS-UCC	2	TCTCCTGAGGTTGTGGGAAT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.196141	45493	45960	649-652	1.000000	80.2
pafI-trnS-UCC_p3	pafI-trnS-UCC	3	TCTCCTGAGGTTGTGGGAAT	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.0	57.8	0.0	5.6	0.237456	45493	45960	599-602	1.000000	80.0
pafI-trnS-UCC_p4	pafI-trnS-UCC	4	TTCTCCTGAGGTTGTGGGAA	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.279934	45493	45960	629-632	1.000000	79.5
pafI-trnS-UCC_p5	pafI-trnS-UCC	5	TTTCTCCTGAGGTTGTGGGA	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.279934	45493	45960	630-633	1.000000	79.5
rps4-trnT-ACA_p1	rps4-trnT-ACA	1	AGTTAGTCCCGGTAAAGCCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	59.1	57.9	0.0	0.0	1.225254	46962	47353	522-544	1.000000	71.4
rps4-trnT-ACA_p2	rps4-trnT-ACA	2	AGTTAGTCCCGGTAAAGCCC	GATGGTCATCGGTTCGACTC	20	20	55.0	55.0	59.1	58.2	0.0	0.0	1.251064	46962	47353	491-513	1.000000	71.3
rps4-trnT-ACA_p3	rps4-trnT-ACA	3	TCACTTCCGGATCTAGGTCT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	56.9	57.9	4.1	0.0	1.258465	46962	47353	548-570	1.000000	71.1
rps4-trnT-ACA_p4	rps4-trnT-ACA	4	CCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	19	20	52.6	55.0	58.1	57.9	14.7	0.0	1.270002	46962	47353	504-526	1.000000	71.1
rps4-trnT-ACA_p5	rps4-trnT-ACA	5	TCACTTCCGGATCTAGGTCT	GATGGTCATCGGTTCGACTC	20	20	50.0	55.0	56.9	58.2	4.1	0.0	1.284276	46962	47353	517-539	1.000000	70.9
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	CACAAAGCAGTCGGTCTCTT	GGTCGAGGCTATCAATGCAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.175361	53409	53554	292-294	1.000000	80.2
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TTCACAAAGCAGTCGGTCTC	GGTCGAGGCTATCAATGCAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.180576	53409	53554	294-296	1.000000	80.2
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TATTGCTTTCATACGGCGGG	GGTCGAGGCTATCAATGCAA	20	20	50.0	50.0	58.4	58.0	13.2	0.0	0.457767	53409	53554	226-228	1.000000	78.5
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	ATTCACAAAGCAGTCGGTCT	GGTCGAGGCTATCAATGCAA	20	20	45.0	50.0	57.4	58.0	0.0	0.0	0.597271	53409	53554	295-297	1.000000	76.8
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	ACAAAGCAGTCGGTCTCTTT	GGTCGAGGCTATCAATGCAA	20	20	45.0	50.0	57.4	58.0	0.0	0.0	0.668505	53409	53554	291-293	1.000000	76.3
rbcL-psbE_p1	rbcL-psbE	1	CGTGAAGCTTGCAAATGGAG	CAACCCGCAATGAATAGGGA	20	20	50.0	50.0	58.0	57.9	2.1	6.4	0.078196	57644	58810	1354-1364	1.000000	81.3
rbcL-psbE_p2	rbcL-psbE	2	CGTGAAGCTTGCAAATGGAG	ACTGACGAATAACCAACCCG	20	20	50.0	50.0	58.0	57.9	2.1	0.0	0.115811	57644	58810	1367-1377	1.000000	81.0
rbcL-psbE_p3	rbcL-psbE	3	GTTCAAACCGGTAGACACGA	CAACCCGCAATGAATAGGGA	20	20	50.0	50.0	57.9	57.9	8.0	6.4	0.189930	57644	58810	1286-1296	1.000000	80.5
rbcL-psbE_p4	rbcL-psbE	4	AGTTCAAACCGGTAGACACG	CAACCCGCAATGAATAGGGA	20	20	50.0	50.0	57.9	57.9	8.0	6.4	0.195816	57644	58810	1287-1297	1.000000	80.5
rbcL-psbE_p5	rbcL-psbE	5	CGTGAAGCTTGCAAATGGAG	ACGAATAACCAACCCGCAAT	20	20	50.0	45.0	58.0	58.2	2.1	0.0	0.197883	57644	58810	1363-1373	1.000000	80.3
psbT-pbf1_p1	psbT-pbf1	1	ACCACGATCGAACCTATGGA	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.2	58.0	24.3	0.0	0.243892	75100	75174	305-305	1.000000	79.1
psbT-pbf1_p2	psbT-pbf1	2	ACCACGATCGAACCTATGGA	CCCTAGTCGCCATCTCCATA	20	20	50.0	55.0	58.2	58.1	24.3	0.0	0.303704	75100	75174	311-311	1.000000	78.6
psbT-pbf1_p3	psbT-pbf1	3	CGATCGAACCTATGGAAGCA	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	57.5	58.0	10.3	0.0	0.524509	75100	75174	301-301	1.000000	76.9
psbT-pbf1_p4	psbT-pbf1	4	ACGATCGAACCTATGGAAGC	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	57.5	58.0	24.3	0.0	0.526224	75100	75174	302-302	1.000000	76.8
psbT-pbf1_p5	psbT-pbf1	5	CGATCGAACCTATGGAAGCA	CCCTAGTCGCCATCTCCATA	20	20	50.0	55.0	57.5	58.1	10.3	0.0	0.584321	75100	75174	307-307	1.000000	76.4
rpl16_p1	rpl16	1	ACCCAATATCTATATATCCATATCC	ACCATTTTATTTGGTGAAATTGCT	25	24	32.0	29.2	52.9	56.3	0.0	0.0	15.835915	82114	83587	1523-1531	1.000000	41.4
rpl16_p2	rpl16	2	ACCCAATATCTATATATCCATATCC	ACCATTTTATTTGGTGAAATTGC	25	23	32.0	30.4	52.9	54.9	0.0	0.0	16.219438	82114	83587	1523-1531	1.000000	41.4
rpl16_p3	rpl16	3	ACCCAATATCTATATATCCATATCC	CCATTTTATTTGGTGAAATTGCT	25	23	32.0	30.4	52.9	54.6	0.0	0.0	16.484827	82114	83587	1522-1530	1.000000	41.4
rpl16_p4	rpl16	4	ACCCAATATCTATATATCCATATCC	TCGATTGACCATTTTATTTGG	25	21	32.0	33.3	52.9	52.5	0.0	0.0	16.650152	82114	83587	1530-1538	1.000000	41.4
rpl16_p5	rpl16	5	ACCCAATATCTATATATCCATATCC	TTCGATTGACCATTTTATTTGG	25	22	32.0	31.8	52.9	53.4	0.0	0.0	16.761079	82114	83587	1531-1539	1.000000	41.4
rpl22-rps19_p1	rpl22-rps19	1	GCGATCTCTTCCAGGTCTTC	TGGTAGACATGCGAGAAACG	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.226630	84845	85049	273-292	1.000000	83.6
rpl22-rps19_p2	rpl22-rps19	2	TAGGCGATCTCTTCCAGGTC	TGGTAGACATGCGAGAAACG	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.315574	84845	85049	276-295	1.000000	82.8
rpl22-rps19_p3	rpl22-rps19	3	ATAGGCGATCTCTTCCAGGT	TGGTAGACATGCGAGAAACG	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.399426	84845	85049	277-296	1.000000	82.1
rpl22-rps19_p4	rpl22-rps19	4	GCGATCTCTTCCAGGTCTTC	GGAGAATTCGCACCTACTCG	20	20	55.0	55.0	57.8	58.2	0.0	11.5	0.375980	84845	85049	299-318	1.000000	81.9
rpl22-rps19_p5	rpl22-rps19	5	GCGATCTCTTCCAGGTCTTC	GGGAGAATTCGCACCTACTC	20	20	55.0	55.0	57.8	57.8	0.0	11.5	0.444031	84845	85049	300-319	1.000000	81.4
rps19_p1	rps19	1	TCGGGCTTTGAAAACAGACA	CGTTTTATTCTTCGTCGCCG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.099313	85050	85328	721-870	1.000000	83.7
rps19_p2	rps19	2	TCGGGCTTTGAAAACAGACA	GGGTAGAGCCGGATCTAAGT	20	20	45.0	55.0	58.0	58.0	0.0	29.9	0.047339	85050	85328	908-1057	1.000000	83.3
rps19_p3	rps19	3	TCGGGCTTTGAAAACAGACA	TGGGTAGAGCCGGATCTAAG	20	20	45.0	55.0	58.0	58.0	0.0	29.9	0.048763	85050	85328	909-1058	1.000000	83.3
rps19_p4	rps19	4	TCGGGCTTTGAAAACAGACA	GAGCCGGATCTAAGTGTTGG	20	20	45.0	55.0	58.0	58.1	0.0	13.7	0.104236	85050	85328	903-1052	1.000000	82.8
rps19_p5	rps19	5	TCGGGCTTTGAAAACAGACA	GAGGGTAAATCGGCCACATT	20	20	45.0	50.0	58.0	57.9	0.0	2.2	0.098344	85050	85328	1021-1170	1.000000	82.5
rps19-rpl2_p1	rps19-rpl2	1	TGGTTAGCCACAAAAGGATT	CGTTTTATTCTTCGTCGCCG	20	20	40.0	50.0	55.1	57.9	0.0	0.0	2.935262	85329	85454	194-324	1.000000	61.8
rps19-rpl2_p2	rps19-rpl2	2	TGGTTAGCCACAAAAGGATT	CCCCTTGGGGTTATCCTACA	20	20	40.0	55.0	55.1	58.1	0.0	16.5	2.979862	85329	85454	253-383	1.000000	61.2
rps19-rpl2_p3	rps19-rpl2	3	TGGTTAGCCACAAAAGGATTT	CGTTTTATTCTTCGTCGCCG	21	20	38.1	50.0	55.9	57.9	0.0	0.0	3.110066	85329	85454	194-324	1.000000	60.4
rps19-rpl2_p4	rps19-rpl2	4	TGGTTAGCCACAAAAGGATTT	CCCCTTGGGGTTATCCTACA	21	20	38.1	55.0	55.9	58.1	0.0	16.5	3.154667	85329	85454	253-383	1.000000	59.8
rps19-rpl2_p5	rps19-rpl2	5	TGGTTAGCCACAAAAGGATT	AAAAACCCATGACCCCTTGG	20	20	40.0	50.0	55.1	58.3	0.0	0.0	3.153186	85329	85454	265-395	1.000000	59.7
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	109466	110068	695-744	1.000000	82.6
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	109466	110068	646-695	1.000000	81.4
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	CGGTCGGCTGTTAACTGATT	20	20	55.0	50.0	57.8	58.3	18.9	15.9	0.493896	109466	110068	665-714	1.000000	80.4
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.526686	109466	110068	689-738	1.000000	80.1
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	GGTTCGAATCCTACTTGGGG	20	20	55.0	55.0	57.8	57.7	18.9	0.0	0.543107	109466	110068	638-687	1.000000	80.0
ycf1_p1	ycf1	1	AGGCTGTACGAAATGAAGTGT	ACATCTAGAACAAAGGATTCTCT	21	23	42.9	34.8	57.6	54.8	0.0	0.0	7.609935	111523	111642	280-280	1.000000	42.3
ycf1_p2	ycf1	2	AGGCTGTACGAAATGAAGTGT	ACATCTAGAACAAAGGATTCTCTT	21	24	42.9	33.3	57.6	55.5	0.0	0.0	7.870031	111523	111642	280-280	1.000000	42.3
ycf1_p3	ycf1	3	GGCTGTACGAAATGAAGTGTC	ACATCTAGAACAAAGGATTCTCT	21	23	47.6	34.8	57.3	54.8	0.0	0.0	7.931602	111523	111642	279-279	1.000000	42.3
ycf1_p4	ycf1	4	AGGCTGTACGAAATGAAGTGT	AGTACATCTAGAACAAAGGATTCTCT	21	26	42.9	34.6	57.6	57.4	0.0	0.0	8.005099	111523	111642	283-283	1.000000	42.3
ycf1_p5	ycf1	5	AGGCTGTACGAAATGAAGTGT	ACATCTAGAACAAAGGATTCTC	21	22	42.9	36.4	57.6	53.3	0.0	0.0	8.110835	111523	111642	280-280	1.000000	42.3
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	CCGCGGTTAAAGCTTTTTCC	20	20	50.0	50.0	58.0	58.3	0.0	19.5	0.314363	126147	126840	806-806	0.500000	57.5
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	CCGCGGTTAAAGCTTTTTCC	20	20	50.0	50.0	58.0	58.3	0.0	19.5	0.315734	126147	126840	807-807	0.500000	57.5
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	TCCTAAGAGCAGCGTGTCTA	CCGCGGTTAAAGCTTTTTCC	20	20	50.0	50.0	57.9	58.3	0.0	19.5	0.430810	126147	126840	816-816	0.500000	56.6
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CCTAAGAGCAGCGTGTCTAC	CCGCGGTTAAAGCTTTTTCC	20	20	55.0	50.0	57.8	58.3	0.0	19.5	0.502828	126147	126840	815-815	0.500000	56.0
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	CTAAGAGCAGCGTGTCTACC	CCGCGGTTAAAGCTTTTTCC	20	20	55.0	50.0	57.8	58.3	0.0	19.5	0.502828	126147	126840	814-814	0.500000	56.0