primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	86	288	257-270	1.000000	85.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	86	288	307-320	1.000000	84.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	86	288	321-334	1.000000	84.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	86	288	320-333	1.000000	84.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	86	288	322-335	1.000000	84.2
psbA_p1	psbA	1	TCTTGTATATACTAAAACCCGGTC	AAATTCGTGTGCTTGGGAGT	24	20	37.5	45.0	55.8	58.0	13.9	0.0	6.236094	289	1350	1137-1137	1.000000	40.0
psbA_p2	psbA	2	TCTTGTATATACTAAAACCCGGTC	TGCTTGGGAGTCCCTGATAA	24	20	37.5	50.0	55.8	58.0	13.9	28.7	6.251937	289	1350	1128-1128	1.000000	40.0
psbA_p3	psbA	3	TCTTGTATATACTAAAACCCGGT	AAATTCGTGTGCTTGGGAGT	23	20	34.8	45.0	54.6	58.0	13.9	0.0	6.405641	289	1350	1137-1137	1.000000	40.0
psbA_p4	psbA	4	TCTTGTATATACTAAAACCCGGT	TGCTTGGGAGTCCCTGATAA	23	20	34.8	50.0	54.6	58.0	13.9	28.7	6.421483	289	1350	1128-1128	1.000000	40.0
psbA_p5	psbA	5	CTTGTATATACTAAAACCCGGTC	AAATTCGTGTGCTTGGGAGT	23	20	39.1	45.0	54.4	58.0	13.9	0.0	6.635963	289	1350	1136-1136	1.000000	40.0
atpH-atpI_p1	atpH-atpI	1	AATCGAAGCAAGCCCTACAG	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	58.0	57.9	0.0	21.3	0.095636	13304	14433	1226-1259	1.000000	81.3
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.0	57.9	1.4	21.3	0.104185	13304	14433	1192-1225	1.000000	81.3
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	57.9	57.9	0.0	21.3	0.178077	13304	14433	1248-1281	1.000000	80.6
atpH-atpI_p4	atpH-atpI	4	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	57.9	57.9	0.0	21.3	0.214244	13304	14433	1208-1241	1.000000	80.4
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.2	57.9	1.4	21.3	0.249724	13304	14433	1193-1226	1.000000	80.1
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AGCCCTGATCCATGAACCTA	CTTACCACTCGGCCATATCG	20	20	50.0	55.0	57.8	57.9	0.0	2.2	0.262735	26811	28048	1337-1351	1.000000	79.0
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	AGCCCTGATCCATGAACCTA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.278870	26811	28048	1377-1391	1.000000	78.9
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GCCCTGATCCATGAACCTAC	CTTACCACTCGGCCATATCG	20	20	55.0	55.0	57.7	57.9	0.0	2.2	0.328416	26811	28048	1336-1350	1.000000	78.5
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	GCCCTGATCCATGAACCTAC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	57.7	58.1	0.0	0.0	0.344551	26811	28048	1376-1390	1.000000	78.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AAAGTTCTTCCGTCAAGCCC	CTTACCACTCGGCCATATCG	20	20	50.0	55.0	58.4	57.9	0.0	2.2	0.460240	26811	28048	1352-1366	1.000000	77.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.217341	28120	28818	785-795	1.000000	79.6
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	28120	28818	745-755	1.000000	79.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	CGATATGGCCGAGTGGTAAG	AGAGTCCACTTCTTCCCCAT	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.416855	28120	28818	846-856	1.000000	77.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	28120	28818	806-816	1.000000	77.8
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	28120	28818	763-773	1.000000	77.3
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	28909	30045	1309-1311	1.000000	67.9
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	28909	30045	1248-1250	1.000000	66.3
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCT	20	21	50.0	42.9	58.2	57.2	0.0	0.0	1.930302	28909	30045	1288-1290	1.000000	64.9
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.2	56.1	0.0	0.0	2.081320	28909	30045	1309-1311	1.000000	63.7
petN-psbM_p5	petN-psbM	5	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	20	21	50.0	42.9	57.6	57.2	0.0	0.0	2.129816	28909	30045	1227-1229	1.000000	63.4
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	31275	32018	843-848	1.000000	79.6
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.2	58.0	0.0	0.0	0.220324	31275	32018	878-883	1.000000	79.2
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273369	31275	32018	808-813	1.000000	78.9
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	20	20	60.0	50.0	58.2	57.9	0.0	0.0	0.317890	31275	32018	843-848	1.000000	78.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	31275	32018	840-845	1.000000	78.5
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	20	20	50.0	45.0	57.9	58.1	4.1	11.5	0.219123	42039	42760	833-842	1.000000	79.8
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	42039	42760	820-829	1.000000	79.3
psaA-ycf3_p3	psaA-ycf3	3	TTCTTGAGAAATGACCCGGC	AGCGCAGAATTGGTTGAAGA	20	20	50.0	45.0	58.2	58.1	0.0	11.5	0.282564	42039	42760	856-865	1.000000	79.2
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	42039	42760	824-833	1.000000	79.1
psaA-ycf3_p5	psaA-ycf3	5	TTCTTGAGAAATGACCCGGC	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.342602	42039	42760	843-852	1.000000	78.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.230316	50872	51998	1269-1279	1.000000	79.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.4	58.1	0.0	0.0	0.715163	50872	51998	1256-1266	1.000000	75.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.806132	50872	51998	1254-1264	1.000000	74.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTTGTTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.3	58.1	0.0	0.0	0.828958	50872	51998	1264-1274	1.000000	74.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAACTCCGGAAAC	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.8	58.1	5.2	0.0	0.936224	50872	51998	1251-1261	1.000000	73.5
ycf4_p1	ycf4	1	AGTTGATGAGAGTTACTTCGGA	GCATTCACTCTCTCAGCTCA	22	20	40.9	50.0	56.6	57.3	0.0	0.0	4.075403	60196	60750	662-662	1.000000	47.8
ycf4_p2	ycf4	2	TGATGAGAGTTACTTCGGAAACA	GCATTCACTCTCTCAGCTCA	23	20	39.1	50.0	57.5	57.3	0.0	0.0	4.131831	60196	60750	659-659	1.000000	47.3
ycf4_p3	ycf4	3	AGTTGATGAGAGTTACTTCGGA	AGCATTCACTCTCTCAGCTC	22	20	40.9	50.0	56.6	57.0	0.0	0.0	4.371674	60196	60750	663-663	1.000000	45.4
ycf4_p4	ycf4	4	AGTTGATGAGAGTTACTTCGGAA	GCATTCACTCTCTCAGCTCA	23	20	39.1	50.0	57.3	57.3	0.0	0.0	4.392972	60196	60750	662-662	1.000000	45.2
ycf4_p5	ycf4	5	TGATGAGAGTTACTTCGGAAACA	AGCATTCACTCTCTCAGCTC	23	20	39.1	50.0	57.5	57.0	0.0	0.0	4.428102	60196	60750	660-660	1.000000	44.9
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTGGC	GCAGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	63351	64271	1096-1101	1.000000	72.0
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	AGCAGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	63351	64271	1097-1102	1.000000	71.2
petA-psbJ_p3	petA-psbJ	3	TTGCGTGTACAAGGCCTTTT	GCAGGTATTCTTGTGATCGGT	20	21	45.0	47.6	58.6	58.1	17.6	0.0	1.699028	63351	64271	1108-1113	1.000000	68.4
petA-psbJ_p4	petA-psbJ	4	TTGCGTGTACAAGGCCTTTT	AGCAGGTATTCTTGTGATCGG	20	21	45.0	47.6	58.6	57.8	17.6	0.0	1.790628	63351	64271	1109-1114	1.000000	67.6
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTGGC	GCAGGTATTCTTGTGATCGG	20	20	50.0	50.0	57.8	56.3	0.0	0.0	1.845120	63351	64271	1096-1101	1.000000	67.2
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	GGTGCCACGGAAAGATATGT	20	20	45.0	50.0	57.9	58.0	5.7	0.0	0.152297	66625	67015	455-461	1.000000	80.7
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	AAAACAAACGCGCTACCAAG	TGTCAACGCATCTGGGAAAA	20	20	45.0	45.0	57.9	58.0	5.7	0.8	0.139645	66625	67015	534-540	1.000000	80.5
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	CAAAACAAACGCGCTACCAA	GGTGCCACGGAAAGATATGT	20	20	45.0	50.0	58.2	58.0	5.7	0.0	0.209555	66625	67015	456-462	1.000000	80.2
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAAAACAAACGCGCTACCAA	TGTCAACGCATCTGGGAAAA	20	20	45.0	45.0	58.2	58.0	5.7	0.8	0.196903	66625	67015	535-541	1.000000	80.1
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TTGTACCCAAAACAAACGCG	TGTCAACGCATCTGGGAAAA	20	20	45.0	45.0	57.8	58.0	3.5	0.8	0.216060	66625	67015	542-548	1.000000	79.9
petB_p1	petB	1	TGTTGAGAAGGTTGCCCAAA	AATGCAGCGGAAATGATTGC	20	20	45.0	45.0	57.8	58.1	0.0	0.0	0.220935	75009	76398	2436-2438	1.000000	78.9
petB_p2	petB	2	TGTTGAGAAGGTTGCCCAAA	GCAGCGGAAATGATTGCATT	20	20	45.0	45.0	57.8	58.1	0.0	0.0	0.220935	75009	76398	2433-2435	1.000000	78.9
petB_p3	petB	3	TTGTTGAGAAGGTTGCCCAA	AATGCAGCGGAAATGATTGC	20	20	45.0	45.0	57.8	58.1	0.0	0.0	0.220935	75009	76398	2437-2439	1.000000	78.9
petB_p4	petB	4	TTGTTGAGAAGGTTGCCCAA	GCAGCGGAAATGATTGCATT	20	20	45.0	45.0	57.8	58.1	0.0	0.0	0.220935	75009	76398	2434-2436	1.000000	78.9
petB_p5	petB	5	TGTTGAGAAGGTTGCCCAAA	CGGCAGATGGATAAAGGCAT	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.249911	75009	76398	2197-2199	1.000000	78.7
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGACGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	20	20	50.0	50.0	58.0	58.2	20.5	16.6	0.199227	112665	113672	1174-1193	1.000000	80.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGACGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	20	20	45.0	50.0	58.0	58.2	20.5	16.6	0.210516	112665	113672	1172-1191	1.000000	80.6
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGACGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	20	20	45.0	50.0	58.0	58.2	20.5	16.6	0.210516	112665	113672	1173-1192	1.000000	80.6
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGACGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	20	20	50.0	50.0	58.0	58.3	20.5	0.0	0.345950	112665	113672	1185-1204	1.000000	79.5
ndhF-rpl32_p5	ndhF-rpl32	5	AAAAGAAGAAGTCCCGCTCC	TAAGGCTGCCCAATATCCCT	20	20	50.0	50.0	57.8	58.2	0.0	16.6	0.353345	112665	113672	1162-1181	1.000000	79.5
ycf1_p1	ycf1	1	AGACAAACGAATGGGATCGA	CCACAAAAATGGCGAACTACA	20	21	45.0	42.9	56.9	57.4	3.5	0.0	2.685477	125240	125359	1197-1203	1.000000	60.0
ycf1_p2	ycf1	2	AGACAAACGAATGGGATCGA	ACCACAAAAATGGCGAACTAC	20	21	45.0	42.9	56.9	57.4	3.5	0.0	2.687191	125240	125359	1198-1204	1.000000	60.0
ycf1_p3	ycf1	3	CCGCGGGTTCTTTTTCTTCT	CCACAAAAATGGCGAACTACA	20	21	50.0	42.9	58.8	57.4	20.1	0.0	2.398844	125240	125359	856-856	0.666667	48.0
ycf1_p4	ycf1	4	CCGCGGGTTCTTTTTCTTCT	ACCACAAAAATGGCGAACTAC	20	21	50.0	42.9	58.8	57.4	20.1	0.0	2.400558	125240	125359	857-857	0.666667	48.0
ycf1_p5	ycf1	5	CGCGGGTTCTTTTTCTTCTT	CCACAAAAATGGCGAACTACA	20	21	45.0	42.9	56.9	57.4	0.0	0.0	2.693717	125240	125359	855-855	0.666667	45.7