primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCACTTAAAAGCCGAG	GCTCAACCTACGGAAACTGT	20	20	50.0	50.0	58.6	57.8	6.4	0.0	0.735856	3935	4708	897-910	1.000000	76.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AGTCGCACTTAAAAGCCGAG	GGAGCTCAACCTACGGAAAC	20	20	50.0	55.0	58.6	58.3	6.4	17.2	0.847318	3935	4708	900-913	1.000000	75.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TAGTCGCACTTAAAAGCCGA	GCTCAACCTACGGAAACTGT	20	20	45.0	50.0	57.3	57.8	3.0	0.0	0.894008	3935	4708	898-911	1.000000	75.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCGCACTTAAAAGCCGAGTA	GCTCAACCTACGGAAACTGT	20	20	45.0	50.0	57.3	57.8	3.0	0.0	0.894008	3935	4708	895-908	1.000000	75.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGTCGCACTTAAAAGCCGAG	AGGAGCTCAACCTACGGAAA	20	20	50.0	50.0	58.6	58.4	6.4	17.2	0.929621	3935	4708	901-914	1.000000	74.9
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	TTAGAGTACCGCCCTGTCAA	CAATTGCTTTGACTCACCGT	20	20	50.0	45.0	58.1	56.9	0.0	0.0	1.137837	24027	25314	1427-1455	1.000000	72.9
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	ATTGGTTAGAGTACCGCCCT	CAATTGCTTTGACTCACCGT	20	20	50.0	45.0	58.1	56.9	0.0	0.0	1.189836	24027	25314	1432-1460	1.000000	72.5
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	TTAGAGTACCGCCCTGTCAA	TCAATTGCTTTGACTCACCG	20	20	50.0	45.0	58.1	56.7	0.0	0.0	1.421455	24027	25314	1428-1456	1.000000	70.6
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	TTAGAGTACCGCCCTGTCAA	CAATTGCTTTGACTCACCGTT	20	21	50.0	42.9	58.1	57.6	0.0	0.0	1.426602	24027	25314	1427-1455	1.000000	70.6
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	ATTGGTTAGAGTACCGCCCT	TCAATTGCTTTGACTCACCG	20	20	50.0	45.0	58.1	56.7	0.0	0.0	1.473454	24027	25314	1433-1461	1.000000	70.2
atpI-atpH_p1	atpI-atpH	1	TAGGTGAATCCATGGAGGGT	TCCAATAGAAGCGAGGCCTA	20	20	50.0	50.0	57.4	57.9	21.3	4.4	0.669200	39066	39737	766-771	1.000000	76.2
atpI-atpH_p2	atpI-atpH	2	TAGGTGAATCCATGGAGGGT	CCTACGGATAATCCAGCAGC	20	20	50.0	55.0	57.4	57.9	21.3	0.0	0.673114	39066	39737	750-755	1.000000	76.2
atpI-atpH_p3	atpI-atpH	3	TAGGTGAATCCATGGAGGGT	GCCTACGGATAATCCAGCAG	20	20	50.0	55.0	57.4	57.9	21.3	0.0	0.673114	39066	39737	751-756	1.000000	76.2
atpI-atpH_p4	atpI-atpH	4	TAGGTGAATCCATGGAGGGT	AACGGAAGCAGCAGAAATCA	20	20	50.0	45.0	57.4	58.1	21.3	0.0	0.690840	39066	39737	727-732	1.000000	76.1
atpI-atpH_p5	atpI-atpH	5	TAGGTGAATCCATGGAGGGT	ACGGAAGCAGCAGAAATCAA	20	20	50.0	45.0	57.4	58.1	21.3	0.0	0.690840	39066	39737	726-731	1.000000	76.1
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	AGGACTGAAAATCCTCGTGC	AAAGTTGGATTGGTTGGCCT	20	20	50.0	45.0	57.9	57.9	0.0	3.3	0.202244	48079	48497	538-540	1.000000	80.3
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	AGGACTGAAAATCCTCGTGC	AAGTTGGATTGGTTGGCCTT	20	20	50.0	45.0	57.9	57.9	0.0	3.3	0.202244	48079	48497	537-539	1.000000	80.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	AGGACTGAAAATCCTCGTGC	AGTTGGATTGGTTGGCCTTT	20	20	50.0	45.0	57.9	57.9	0.0	3.3	0.202244	48079	48497	536-538	1.000000	80.3
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GACTGAAAATCCTCGTGCCA	AAGTTGGATTGGTTGGCCTT	20	20	50.0	45.0	58.2	57.9	0.0	3.3	0.293229	48079	48497	535-537	1.000000	79.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GACTGAAAATCCTCGTGCCA	AGTTGGATTGGTTGGCCTTT	20	20	50.0	45.0	58.2	57.9	0.0	3.3	0.293229	48079	48497	534-536	1.000000	79.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCCAAAGCTATGGAAAT	TGGGTTCTTGGAACAGTTCG	20	20	50.0	50.0	58.2	58.0	4.4	7.2	0.244793	50208	51551	1527-1535	1.000000	79.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCCAAAGCTATGGAAAT	TGCATGTTGGGTTCTTGGAA	20	20	50.0	45.0	58.2	57.9	4.4	0.0	0.285756	50208	51551	1534-1542	1.000000	79.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTATCGGGGCCAAAGCTATG	TGGGTTCTTGGAACAGTTCG	20	20	50.0	50.0	57.7	58.0	4.4	7.2	0.308559	50208	51551	1532-1540	1.000000	79.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TTATCGGGGCCAAAGCTATG	TGCATGTTGGGTTCTTGGAA	20	20	50.0	45.0	57.7	57.9	4.4	0.0	0.349522	50208	51551	1539-1547	1.000000	78.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCCAAAGCTATGGAAAT	GGGTTCTTGGAACAGTTCGA	20	20	50.0	50.0	58.2	57.8	4.4	7.2	0.456430	50208	51551	1526-1534	1.000000	77.8
accD-rps12_p1	accD-rps12	1	TATCGTATGTGCTTCTGGCG	CCGTTTCAGCTTACGATCCA	20	20	50.0	50.0	57.9	58.0	0.0	12.8	0.138784	59900	61783	2370-2597	1.000000	81.6
accD-rps12_p2	accD-rps12	2	TTGGCTAACGTCACGATTGT	CCGTTTCAGCTTACGATCCA	20	20	45.0	50.0	57.8	58.0	2.9	12.8	0.162417	59900	61783	2201-2428	1.000000	81.6
accD-rps12_p3	accD-rps12	3	TGTTGGCTAACGTCACGATT	CCAATGGCTTTTGCTACTGC	20	20	45.0	50.0	57.8	58.0	2.9	0.0	0.158263	59900	61783	2789-2958	0.800000	72.9
accD-rps12_p4	accD-rps12	4	TTGGCTAACGTCACGATTGT	CCAATGGCTTTTGCTACTGC	20	20	45.0	50.0	57.8	58.0	2.9	0.0	0.158263	59900	61783	2787-2956	0.800000	72.9
accD-rps12_p5	accD-rps12	5	TATCGTATGTGCTTCTGGCG	CCAATGGCTTTTGCTACTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.134631	59900	61783	2956-2988	0.600000	64.5
rps18_p1	rps18	1	ATCCGATTCGATCCAGGAGT	AACAAAATCTGTCACTGTCA	20	20	50.0	35.0	58.0	53.0	7.8	0.0	5.046239	63138	63629	559-589	1.000000	44.7
rps18_p2	rps18	2	ATCCGATTCGATCCAGGAGT	ACAAAATCTGTCACTGTCAA	20	20	50.0	35.0	58.0	53.0	7.8	0.0	5.046239	63138	63629	558-588	1.000000	44.7
rps18_p3	rps18	3	ATCCGATTCGATCCAGGAGT	AACAAAATCTGTCACTGTCAA	20	21	50.0	33.3	58.0	53.9	7.8	0.0	5.130897	63138	63629	559-589	1.000000	44.7
rps18_p4	rps18	4	AGATCCGATTCGATCCAGGA	AACAAAATCTGTCACTGTCA	20	20	50.0	35.0	57.7	53.0	7.8	0.0	5.338183	63138	63629	561-591	1.000000	44.7
rps18_p5	rps18	5	AGATCCGATTCGATCCAGGA	ACAAAATCTGTCACTGTCAA	20	20	50.0	35.0	57.7	53.0	7.8	0.0	5.338183	63138	63629	560-590	1.000000	44.7
psbJ-petA_p1	psbJ-petA	1	TCCGGATTAGGATCATCCCT	TAGCATCTGTCGTTTTGGCA	20	20	50.0	45.0	57.0	57.8	0.0	0.0	1.200037	67446	68426	1078-1111	1.000000	73.4
psbJ-petA_p2	psbJ-petA	2	TCCGGATTAGGATCATCCCT	TCTGTCGTTTTGGCACAGAT	20	20	50.0	45.0	57.0	57.7	0.0	0.0	1.277189	67446	68426	1073-1106	1.000000	72.8
psbJ-petA_p3	psbJ-petA	3	CGGAAGGATCCCTCTTTGGT	TAGCATCTGTCGTTTTGGCA	20	20	55.0	45.0	59.1	57.8	8.6	0.0	1.264907	67446	68426	1163-1196	1.000000	72.7
psbJ-petA_p4	psbJ-petA	4	TCCGGATTAGGATCATCCCT	CATCTGTCGTTTTGGCACAG	20	20	50.0	50.0	57.0	57.7	0.0	0.0	1.343449	67446	68426	1075-1108	1.000000	72.3
psbJ-petA_p5	psbJ-petA	5	CGGAAGGATCCCTCTTTGGT	TCTGTCGTTTTGGCACAGAT	20	20	55.0	45.0	59.1	57.7	8.6	0.0	1.342059	67446	68426	1158-1191	1.000000	72.1
rpl36-infA_p1	rpl36-infA	1	ACGAACAGAAGCACGGATTT	ATGATGATGATGCTGATGTT	20	20	45.0	35.0	58.1	52.7	0.0	0.0	5.444903	79880	80471	640-645	1.000000	43.7
rpl36-infA_p2	rpl36-infA	2	ACGAACAGAAGCACGGATTT	TGATGATGATGCTGATGTTATCT	20	23	45.0	34.8	58.1	55.6	0.0	0.0	5.518861	79880	80471	639-644	1.000000	43.7
rpl36-infA_p3	rpl36-infA	3	ACGAACAGAAGCACGGATTT	TGATGATGATGCTGATGTTATCTT	20	24	45.0	33.3	58.1	56.3	0.0	0.0	5.818884	79880	80471	639-644	1.000000	43.7
rpl36-infA_p4	rpl36-infA	4	TTTACGAACAGAAGCACGGA	ATGATGATGATGCTGATGTT	20	20	45.0	35.0	57.5	52.7	0.0	0.0	5.836341	79880	80471	643-648	1.000000	43.7
rpl36-infA_p5	rpl36-infA	5	TTTACGAACAGAAGCACGGA	TGATGATGATGCTGATGTTATCT	20	23	45.0	34.8	57.5	55.6	0.0	0.0	5.910299	79880	80471	642-647	1.000000	43.7
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	TACCATTCAGCCATGGATGC	ACCAAAATGTTTTTCGGTGT	20	20	50.0	35.0	58.0	54.5	25.4	0.9	3.519302	86963	87705	788-796	1.000000	55.1
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	AGGAATTGAACCCGCAACTT	ACCAAAATGTTTTTCGGTGT	20	20	45.0	35.0	58.0	54.5	0.0	0.9	3.509399	86963	87705	831-839	1.000000	55.0
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	TACCATTCAGCCATGGATGC	ACCAAAATGTTTTTCGGTGTT	20	21	50.0	33.3	58.0	55.3	25.4	0.9	3.679730	86963	87705	788-796	1.000000	53.8
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	AGGAATTGAACCCGCAACTT	AACCAAAATGTTTTTCGGTGT	20	21	45.0	33.3	58.0	55.3	0.0	2.2	3.669827	86963	87705	832-840	1.000000	53.7
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	AGGAATTGAACCCGCAACTT	ACCAAAATGTTTTTCGGTGTT	20	21	45.0	33.3	58.0	55.3	0.0	0.9	3.669827	86963	87705	831-839	1.000000	53.7
ycf2_p1	ycf2	1	TCAGAGAACCCAGGACCAAT	AGCATCTTGTCGCCTTTTCA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.148573	88478	88597	804-939	1.000000	84.2
ycf2_p2	ycf2	2	TCAGAGAACCCAGGACCAAT	TGAAAAGCATCTTGTCGCCT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.148573	88478	88597	809-944	1.000000	84.1
ycf2_p3	ycf2	3	TCAGAGAACCCAGGACCAAT	TCCATCCGGGATCTGATGAA	20	20	50.0	50.0	58.0	57.9	0.0	6.5	0.146432	88478	88597	916-1051	1.000000	83.6
ycf2_p4	ycf2	4	ATCTCAGAGAACCCAGGACC	AGCATCTTGTCGCCTTTTCA	20	20	55.0	45.0	58.1	58.1	3.6	0.0	0.231008	88478	88597	807-942	1.000000	83.5
ycf2_p5	ycf2	5	ATCTCAGAGAACCCAGGACC	TCCATCCGGGATCTGATGAA	20	20	55.0	50.0	58.1	57.9	3.6	6.5	0.228867	88478	88597	919-1054	1.000000	82.9
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	CGGAAGGATTGAATAAGAATAACA	AAATGGTAGACACGCGAGAC	24	20	33.3	50.0	55.0	58.0	0.0	15.9	6.991599	94495	95164	769-779	1.000000	42.0
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	CGGAAGGATTGAATAAGAATAACA	TGAAATGGTAGACACGCGAG	24	20	33.3	50.0	55.0	58.0	0.0	15.9	6.992970	94495	95164	771-781	1.000000	42.0
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	CGGAAGGATTGAATAAGAATAACA	AATCTCGTGCTAAACAGCGT	24	20	33.3	45.0	55.0	57.9	0.0	0.0	7.064055	94495	95164	745-755	1.000000	42.0
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	CGGAAGGATTGAATAAGAATAACA	TGGTGAAATGGTAGACACGC	24	20	33.3	50.0	55.0	58.2	0.0	0.0	7.180435	94495	95164	774-784	1.000000	42.0
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	CGGAAGGATTGAATAAGAATAACA	GGGATGCCTTGATGGTGAAA	24	20	33.3	50.0	55.0	58.1	0.0	0.0	7.132465	94495	95164	786-796	1.000000	41.9
trnV-GAC-rrn16_p1	trnV-GAC-rrn16	1	TCGGTTCAAATCTGATTATCCCT	CGACTTGCATGTGTTAAGCA	23	20	39.1	45.0	57.4	57.0	2.8	10.4	4.597874	100776	101613	545-926	1.000000	55.2
trnV-GAC-rrn16_p2	trnV-GAC-rrn16	2	TCGGTTCAAATCTGATTATCCCT	CATGTGTTAAGCATGCCGC	23	19	39.1	52.6	57.4	58.1	2.8	15.7	4.682322	100776	101613	538-919	1.000000	54.5
trnV-GAC-rrn16_p3	trnV-GAC-rrn16	3	TCGGTTCAAATCTGATTATCCCT	GAGCCAGGATCAAACTCTCA	23	20	39.1	50.0	57.4	56.9	2.8	0.0	4.698125	100776	101613	506-887	1.000000	54.4
trnV-GAC-rrn16_p4	trnV-GAC-rrn16	4	TCGGTTCAAATCTGATTATCCCT	TGAGCCAGGATCAAACTCTC	23	20	39.1	50.0	57.4	56.9	2.8	4.4	4.698125	100776	101613	507-888	1.000000	54.4
trnV-GAC-rrn16_p5	trnV-GAC-rrn16	5	TCGGTTCAAATCTGATTATCCCT	CTGAGCCAGGATCAAACTCT	23	20	39.1	50.0	57.4	56.9	2.8	9.2	4.706591	100776	101613	508-889	1.000000	54.3
rrn16-trnI-GAU_p1	rrn16-trnI-GAU	1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	20	20	55.0	50.0	57.7	58.2	7.8	0.0	0.485056	103125	104216	1164-1192	1.000000	81.8
rrn16-trnI-GAU_p2	rrn16-trnI-GAU	2	AGTGACTGGAGTGAAGTCGT	GGCACAACGACGCAATTATC	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.496177	103125	104216	1186-1214	1.000000	81.6
rrn16-trnI-GAU_p3	rrn16-trnI-GAU	3	AAGTCGTAACAAGGTAGCCG	GGCACAACGACGCAATTATC	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.548742	103125	104216	1173-1201	1.000000	81.3
rrn16-trnI-GAU_p4	rrn16-trnI-GAU	4	AACAAGGTAGCCGTACTGGA	GGCACAACGACGCAATTATC	20	20	50.0	50.0	58.4	58.2	7.8	0.0	0.553655	103125	104216	1166-1194	1.000000	81.3
rrn16-trnI-GAU_p5	rrn16-trnI-GAU	5	ACAAGGTAGCCGTACTGGAA	GGCACAACGACGCAATTATC	20	20	50.0	50.0	58.4	58.2	7.8	0.0	0.553655	103125	104216	1165-1193	1.000000	81.3
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	GTCGTAGGTTCAAATCCTATTTGA	20	24	55.0	37.5	57.8	56.8	18.9	10.3	5.437352	109973	110513	631-688	1.000000	42.1
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	GTCGTAGGTTCAAATCCTATTTGAG	20	25	55.0	40.0	57.8	57.8	18.9	13.2	5.451337	109973	110513	631-688	1.000000	42.1
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	GAACAGAACAGATCCCGTGG	GTCGTAGGTTCAAATCCTATTTGA	20	24	55.0	37.5	58.3	56.8	0.0	10.3	5.494592	109973	110513	668-725	1.000000	42.0
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	GGAACAGAACAGATCCCGTG	GTCGTAGGTTCAAATCCTATTTGA	20	24	55.0	37.5	58.3	56.8	0.0	10.3	5.494592	109973	110513	669-726	1.000000	42.0
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	GAACAGAACAGATCCCGTGG	GTCGTAGGTTCAAATCCTATTTGAG	20	25	55.0	40.0	58.3	57.8	0.0	13.2	5.508577	109973	110513	668-725	1.000000	42.0
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	CATCCGGCGACAGTACTTAG	GAAGACTTGGATCGGACCAC	20	20	55.0	55.0	57.9	58.0	9.3	0.0	0.132656	132434	133356	1822-1961	1.000000	87.2
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	CATCCGGCGACAGTACTTAG	AATTGGTTACCCGACCCCTA	20	20	55.0	50.0	57.9	58.0	9.3	0.0	0.151404	132434	133356	1860-1999	1.000000	87.0
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	CATCCGGCGACAGTACTTAG	ACTTGGATCGGACCACCTTA	20	20	55.0	50.0	57.9	58.1	9.3	1.3	0.174132	132434	133356	1818-1957	1.000000	86.9
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	CATCCGGCGACAGTACTTAG	TTGGATCGGACCACCTTAGT	20	20	55.0	50.0	57.9	58.1	9.3	1.3	0.174132	132434	133356	1816-1955	1.000000	86.9
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	GGGGTTCTGCCATAACACAT	GAAGACTTGGATCGGACCAC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.155416	132434	133356	1984-2123	1.000000	86.8
chlL-trnH-GUG_p1	chlL-trnH-GUG	1	ACCAATTCCGCCTTTTCCAT	GACGTAGCCAAGTGGTTCAA	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.189111	136053	136479	535-548	1.000000	80.4
chlL-trnH-GUG_p2	chlL-trnH-GUG	2	GTCTGGCTAGGGCAATTGAA	GACGTAGCCAAGTGGTTCAA	20	20	50.0	50.0	57.9	58.1	10.3	0.0	0.255073	136053	136479	578-591	1.000000	79.8
chlL-trnH-GUG_p3	chlL-trnH-GUG	3	TTTTCACCACGTCTGGCTAG	GACGTAGCCAAGTGGTTCAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.290120	136053	136479	588-601	1.000000	79.4
chlL-trnH-GUG_p4	chlL-trnH-GUG	4	ACCAATTCCGCCTTTTCCAT	ATTATTTGGGGCGGACGTAG	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.312652	136053	136479	548-561	1.000000	79.4
chlL-trnH-GUG_p5	chlL-trnH-GUG	5	ACCAATTCCGCCTTTTCCAT	CTATTATTTGGGGCGGACGT	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.312652	136053	136479	550-563	1.000000	79.4