primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	300	577	394-395	1.000000	78.9
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	300	577	408-409	1.000000	78.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.107227	300	577	456-456	0.500000	59.1
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.140446	300	577	406-406	0.500000	58.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.149276	300	577	409-409	0.500000	58.8
psbA_p1	psbA	1	AGGAGCAATAACAAACCTCTTGA	AGAATTCGTGTGCTTGGGAG	23	20	39.1	50.0	58.0	58.2	0.0	0.0	3.200949	578	1639	1209-1209	1.000000	54.4
psbA_p2	psbA	2	AGGAGCAATAACAAACCTCTTGA	AGAGAATTCGTGTGCTTGGG	23	20	39.1	50.0	58.0	58.2	0.0	0.0	3.200949	578	1639	1211-1211	1.000000	54.4
psbA_p3	psbA	3	AGGAGCAATAACAAACCTCTTGA	GAGAATTCGTGTGCTTGGGA	23	20	39.1	50.0	58.0	58.2	0.0	0.0	3.206051	578	1639	1210-1210	1.000000	54.4
psbA_p4	psbA	4	AGGAGCAATAACAAACCTCTTGA	AATTCGTGTGCTTGGGAGTC	23	20	39.1	50.0	58.0	58.5	0.0	0.0	3.490704	578	1639	1207-1207	1.000000	52.1
psbA_p5	psbA	5	AGGAGCAATAACAAACCTCTTGA	GAATTCGTGTGCTTGGGAGT	23	20	39.1	50.0	58.0	58.5	0.0	0.0	3.490704	578	1639	1208-1208	1.000000	52.1
matK_p1	matK	1	CCATTTCTACGATCGCATAACC	TGAGTCTTACTTGTTCCGAGG	22	21	45.5	47.6	57.4	57.1	16.6	0.0	4.583663	2182	3696	1657-1657	1.000000	43.3
matK_p2	matK	2	CCATTTCTACGATCGCATAACC	GAGTCTTACTTGTTCCGAGGT	22	21	45.5	47.6	57.4	57.1	16.6	0.0	4.585580	2182	3696	1656-1656	1.000000	43.3
matK_p3	matK	3	TTTCCATTTCTACGATCGCA	TGAGTCTTACTTGTTCCGAGG	20	21	40.0	47.6	55.2	57.1	16.6	0.0	4.745140	2182	3696	1660-1660	1.000000	42.0
matK_p4	matK	4	TTTCCATTTCTACGATCGCA	GAGTCTTACTTGTTCCGAGGT	20	21	40.0	47.6	55.2	57.1	16.6	0.0	4.747057	2182	3696	1659-1659	1.000000	42.0
matK_p5	matK	5	CCATTTCTACGATCGCATAACC	AGTCTTACTTGTTCCGAGGT	22	20	45.5	45.0	57.4	55.8	16.6	0.0	4.831269	2182	3696	1655-1655	1.000000	41.3
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	3697	4400	835-835	1.000000	72.4
matK-trnK-UUU_p2	matK-trnK-UUU	2	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	3697	4400	834-834	1.000000	72.4
matK-trnK-UUU_p3	matK-trnK-UUU	3	CCATGATCATGAGCAAGTGC	TTGGTTTGGATTGCGTTGTG	20	20	50.0	45.0	57.2	57.8	32.7	0.0	0.992219	3697	4400	929-929	1.000000	72.1
matK-trnK-UUU_p4	matK-trnK-UUU	4	CCATGATCATGAGCAAGTGC	TTGGGTTGCTAACTCAACGG	20	20	50.0	50.0	57.2	58.4	32.7	9.4	1.176288	3697	4400	850-850	1.000000	70.6
matK-trnK-UUU_p5	matK-trnK-UUU	5	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	57.2	57.5	32.7	0.0	1.237069	3697	4400	848-848	1.000000	70.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.186962	4438	5392	1027-1027	1.000000	78.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.186962	4438	5392	1026-1026	1.000000	78.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.3	58.0	5.3	0.0	0.301348	4438	5392	1036-1036	1.000000	77.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4438	5392	1082-1082	1.000000	77.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4438	5392	1081-1081	1.000000	77.1
rps16_p1	rps16	1	AGAAACAAAAAGGTTATACAAGGCT	TATGTACCGACCGAACCGAT	25	20	32.0	50.0	57.4	58.3	0.0	0.0	5.918149	5393	6528	1273-1273	1.000000	40.0
rps16_p2	rps16	2	AGAAACAAAAAGGTTATACAAGGCT	CCGACCGAACCGATGATTAT	25	20	32.0	50.0	57.4	57.6	0.0	0.0	6.031156	5393	6528	1267-1267	1.000000	40.0
rps16_p3	rps16	3	ACAAAAAGGTTATACAAGGCT	TATGTACCGACCGAACCGAT	21	20	33.3	50.0	53.3	58.3	0.0	0.0	6.032038	5393	6528	1269-1269	1.000000	40.0
rps16_p4	rps16	4	AGAAACAAAAAGGTTATACAAGGCT	ATTATGTACCGACCGAACCG	25	20	32.0	50.0	57.4	57.5	0.0	0.0	6.094395	5393	6528	1275-1275	1.000000	40.0
rps16_p5	rps16	5	GGAAATAGCAAAGAAACAAAAAGGT	TATGTACCGACCGAACCGAT	25	20	32.0	50.0	57.2	58.3	0.0	0.0	6.107039	5393	6528	1284-1284	1.000000	40.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6529	7327	883-883	1.000000	77.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	23.3	16.9	0.491095	6529	7327	893-893	1.000000	76.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GCTTTCTGCCACATCGTTTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.6	58.1	0.0	16.9	0.498377	6529	7327	860-860	1.000000	76.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.4	58.1	23.3	16.9	0.499524	6529	7327	894-894	1.000000	76.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	GTGTCGACTAGAAATGGGGC	20	20	50.0	55.0	58.2	58.3	0.0	17.9	0.551081	6529	7327	945-945	1.000000	75.6
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	AATCAAACCGAAAGACCCCT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.976483	8569	9219	762-762	1.000000	72.2
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	CAATCCGACGCTTTAGTCCA	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.976483	8569	9219	761-761	1.000000	72.2
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	AATCCGACGCTTTAGTCCAC	AATCAAACCGAAAGACCCCT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.977871	8569	9219	761-761	1.000000	72.2
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	AATCCGACGCTTTAGTCCAC	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.977871	8569	9219	760-760	1.000000	72.2
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	ACGGAAAGAGAGGGATTCGA	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	58.2	57.0	0.0	0.0	1.123249	8569	9219	815-815	1.000000	71.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	33578	34095	605-605	1.000000	78.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	33578	34095	603-603	1.000000	78.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	33578	34095	659-659	1.000000	78.8
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	33578	34095	584-584	1.000000	78.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	33578	34095	582-582	1.000000	78.5
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.7	57.8	0.0	1.1	0.446564	38902	39351	599-608	1.000000	77.6
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.3	57.8	0.0	1.1	0.479849	38902	39351	585-594	1.000000	77.4
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	20	20	45.0	50.0	57.0	57.8	0.0	1.1	1.188961	38902	39351	555-564	1.000000	71.8
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	AGGGTTGGTCGGGTAACAAA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	59.2	57.8	0.0	1.1	1.324496	38902	39351	586-595	1.000000	70.6
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	ATTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	21	20	47.6	50.0	58.2	57.8	0.0	1.1	1.369583	38902	39351	600-609	1.000000	70.2
rpoA_p1	rpoA	1	TCGGAATATATGGATTGTGCT	CCACATAATGGCTGTAGGC	21	19	38.1	52.6	54.6	55.7	0.0	0.0	7.691301	81873	82868	1209-1210	1.000000	40.1
rpoA_p2	rpoA	2	TTCGGAATATATGGATTGTGCT	CCACATAATGGCTGTAGGC	22	19	36.4	52.6	55.4	55.7	0.0	0.0	7.882020	81873	82868	1210-1211	1.000000	40.1
rpoA_p3	rpoA	3	TGGATTGTGCTAAAGATTCAAGA	CCACATAATGGCTGTAGGC	23	19	34.8	52.6	56.2	55.7	0.0	0.0	8.080501	81873	82868	1199-1200	1.000000	40.1
rpoA_p4	rpoA	4	TGGACCTATTCGGAATATATGGA	CCACATAATGGCTGTAGGC	23	19	39.1	52.6	56.1	55.7	16.9	0.0	8.143211	81873	82868	1218-1219	1.000000	40.1
rpoA_p5	rpoA	5	TCGGAATATATGGATTGTGC	CCACATAATGGCTGTAGGC	20	19	40.0	52.6	52.9	55.7	0.0	0.0	8.329224	81873	82868	1209-1210	1.000000	40.1