primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	trnH-GTG-psbA	1	CTGCCTTAATCCACTTGGCT	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	133	304	300-658	1.000000	79.4
trnH-GTG-psbA_p2	trnH-GTG-psbA	2	CTGCCTTAATCCACTTGGCT	AAGCCCCATCTACAAATGGC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.354286	133	304	223-581	1.000000	79.4
trnH-GTG-psbA_p3	trnH-GTG-psbA	3	CTGCCTTAATCCACTTGGCT	ATCAACCGTGCTAACCTTGG	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.313573	133	304	302-660	1.000000	79.3
trnH-GTG-psbA_p4	trnH-GTG-psbA	4	ACTGCCTTAATCCACTTGGC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.333428	133	304	301-659	1.000000	79.2
trnH-GTG-psbA_p5	trnH-GTG-psbA	5	ACTGCCTTAATCCACTTGGC	ATCAACCGTGCTAACCTTGG	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.339360	133	304	303-661	1.000000	79.1
psbI-trnS-GCT_p1	psbI-trnS-GCT	1	CCCCCATGCTTACTCTCAAA	TAAAGCGGCGGATTGCTAAT	20	20	50.0	45.0	57.2	58.0	0.0	9.8	0.845151	7572	7711	324-324	0.500000	53.2
psbI-trnS-GCT_p2	psbI-trnS-GCT	2	CCCCCATGCTTACTCTCAAA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	57.2	58.1	0.0	0.0	0.916392	7572	7711	348-348	0.500000	52.7
psbI-trnS-GCT_p3	psbI-trnS-GCT	3	CCCCCATGCTTACTCTCAAA	ATGGCTGAGTGGACTAAAGC	20	20	50.0	50.0	57.2	57.6	0.0	5.7	1.216893	7572	7711	338-338	0.500000	50.3
psbI-trnS-GCT_p4	psbI-trnS-GCT	4	CCCCCATGCTTACTCTCAAA	GAGAGATGGCTGAGTGGACT	20	20	50.0	55.0	57.2	58.5	0.0	0.0	1.322907	7572	7711	343-343	0.500000	49.4
psbI-trnS-GCT_p5	psbI-trnS-GCT	5	CCCCATGCTTACTCTCAAACT	TAAAGCGGCGGATTGCTAAT	21	20	47.6	45.0	57.6	58.0	0.0	9.8	1.394735	7572	7711	323-323	0.500000	48.8
rpoC2_p1	rpoC2	1	ACGGTCGAAGCCATTACTTC	CCCATTTCGAGGACTCACAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.021706	15998	16117	803-803	0.500000	59.8
rpoC2_p2	rpoC2	2	ACGGTCGAAGCCATTACTTC	ATTGGTTGTGCTCGAAAGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.024714	15998	16117	952-952	0.500000	59.8
rpoC2_p3	rpoC2	3	ACGGTCGAAGCCATTACTTC	CCCGGTTCCCCAATAGATTG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.024714	15998	16117	1093-1093	0.500000	59.8
rpoC2_p4	rpoC2	4	GGGTCGATCTGGTCTTTCAC	CCCATTTCGAGGACTCACAG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.027276	15998	16117	689-689	0.500000	59.8
rpoC2_p5	rpoC2	5	GGGTCGATCTGGTCTTTCAC	ATTGGTTGTGCTCGAAAGGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.030284	15998	16117	838-838	0.500000	59.8
psbM_p1	psbM	1	CGAGAGCAACTAGAGTCGTC	CGTGCTGCTCTCACTAAAGT	20	20	55.0	50.0	57.6	57.9	0.0	0.0	0.517142	28663	28779	1245-2469	1.000000	79.7
psbM_p2	psbM	2	TGATGATTCTAGCCCGCTCA	CGTGCTGCTCTCACTAAAGT	20	20	50.0	50.0	58.6	57.9	0.0	0.0	0.658571	28663	28779	867-1642	1.000000	79.1
psbM_p3	psbM	3	CCCTCTCTACTGTTCAGGGA	CGTGCTGCTCTCACTAAAGT	20	20	55.0	50.0	57.5	57.9	4.8	0.0	0.605730	28663	28779	1309-2533	1.000000	78.9
psbM_p4	psbM	4	TCCCTCTCTACTGTTCAGGG	CGTGCTGCTCTCACTAAAGT	20	20	55.0	50.0	57.5	57.9	4.8	0.0	0.605730	28663	28779	1310-2534	1.000000	78.9
psbM_p5	psbM	5	CGAGAGCAACTAGAGTCGTC	ATAGACAATAGCCGTGCTGC	20	20	55.0	50.0	57.6	58.1	0.0	8.4	0.574373	28663	28779	1257-1257	0.500000	55.4
psbC-trnS-TGA_p1	psbC-trnS-TGA	1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.434339	34517	34711	330-345	1.000000	82.9
psbC-trnS-TGA_p2	psbC-trnS-TGA	2	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	57.6	58.1	33.4	0.0	0.502562	34517	34711	329-344	1.000000	82.4
psbC-trnS-TGA_p3	psbC-trnS-TGA	3	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.504584	34517	34711	341-356	1.000000	82.3
psbC-trnS-TGA_p4	psbC-trnS-TGA	4	AGCTGCAGCAGGATTTGAAA	GGGTTCGAATCCCTCTCTCT	20	20	45.0	55.0	58.4	58.2	38.2	17.2	0.592592	34517	34711	276-291	1.000000	82.1
psbC-trnS-TGA_p5	psbC-trnS-TGA	5	GCTGCAGCAGGATTTGAAAA	GATGGCCGAGTGGTTGATAG	20	20	45.0	55.0	57.6	58.1	33.4	0.0	0.572807	34517	34711	340-355	1.000000	81.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	GAAAGCTGGATAGGATGGCC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.148958	47671	48406	845-883	1.000000	80.8
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GCCCAAGCCCTCTAGTAGTA	GAAAGCTGGATAGGATGGCC	20	20	55.0	55.0	57.9	58.1	0.0	0.0	0.180690	47671	48406	926-964	1.000000	80.4
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.243485	47671	48406	877-915	1.000000	80.0
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GCCCAAGCCCTCTAGTAGTA	ATCATCCACGCTTGAAACGT	20	20	55.0	45.0	57.9	58.2	0.0	0.0	0.275218	47671	48406	958-996	1.000000	79.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TTTGATGCCTGAAAGCTGGA	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.343105	47671	48406	855-893	1.000000	79.3
atpB-rbcL_p1	atpB-rbcL	1	CATCCAGTACCGGACCAATG	GGTGACATAAGTCCCTCCCT	20	20	55.0	55.0	58.0	58.1	9.5	0.0	0.165500	53501	54309	912-935	1.000000	81.8
atpB-rbcL_p2	atpB-rbcL	2	TCTTCAAGTGCGGAAACCTC	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.240106	53501	54309	865-888	1.000000	81.4
atpB-rbcL_p3	atpB-rbcL	3	ATCCAGTACCGGACCAATGA	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	58.1	58.1	9.5	0.0	0.244930	53501	54309	911-934	1.000000	81.2
atpB-rbcL_p4	atpB-rbcL	4	TCCAGTACCGGACCAATGAT	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	58.1	58.1	9.5	0.0	0.244930	53501	54309	910-933	1.000000	81.2
atpB-rbcL_p5	atpB-rbcL	5	ACTACATCCAGTACCGGACC	GGTGACATAAGTCCCTCCCT	20	20	55.0	55.0	58.2	58.1	9.5	0.0	0.346223	53501	54309	916-939	1.000000	80.3
psbB_p1	psbB	1	TCCCTCAATCCTTCTTGGGT	ACCGCTTGTGGAACTTCAAT	20	20	50.0	45.0	57.9	58.0	0.0	7.3	0.078500	60483	62009	1297-2139	1.000000	91.4
psbB_p2	psbB	2	TTGGGCAAAAGCAGACGTAT	ACCGCTTGTGGAACTTCAAT	20	20	45.0	45.0	58.1	58.0	0.0	7.3	0.114457	60483	62009	1756-2593	1.000000	91.1
psbB_p3	psbB	3	TCCCTCAATCCTTCTTGGGT	AAATGGCAATTGGCGCATAC	20	20	50.0	45.0	57.9	58.1	0.0	25.0	0.121131	60483	62009	1258-2100	1.000000	91.0
psbB_p4	psbB	4	TCCCTCAATCCTTCTTGGGT	CTGACCAGGAACAGGACCTA	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.121172	60483	62009	1544-2386	1.000000	91.0
psbB_p5	psbB	5	TTGGGCAAAAGCAGACGTAT	AAATGGCAATTGGCGCATAC	20	20	45.0	45.0	58.1	58.1	0.0	25.0	0.157088	60483	62009	1717-2554	1.000000	90.7
trnP-TGG-psaJ_p1	trnP-TGG-psaJ	1	GCCCATAGGAGTAGGTAGGG	ACTTAACACAGGTGCCACAG	20	20	60.0	50.0	58.1	58.0	0.0	0.0	0.092701	66299	66611	447-915	1.000000	80.8
trnP-TGG-psaJ_p2	trnP-TGG-psaJ	2	ACCCGTGACATTTTGTACCC	ACTTAACACAGGTGCCACAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.132436	66299	66611	413-881	1.000000	80.5
trnP-TGG-psaJ_p3	trnP-TGG-psaJ	3	CCCGTGACATTTTGTACCCA	ACTTAACACAGGTGCCACAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.133780	66299	66611	412-880	1.000000	80.5
trnP-TGG-psaJ_p4	trnP-TGG-psaJ	4	ACAGGATTTGAACCCGTGAC	ACTTAACACAGGTGCCACAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.140472	66299	66611	424-892	1.000000	80.5
trnP-TGG-psaJ_p5	trnP-TGG-psaJ	5	CAGGATTTGAACCCGTGACA	ACTTAACACAGGTGCCACAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.141805	66299	66611	423-891	1.000000	80.4
petD-rpoA_p1	petD-rpoA	1	GGTTGGGTATTGGAGCAACA	GCATTTCGTAATGGATTTACCGA	20	23	50.0	39.1	58.1	57.6	0.0	15.4	3.494654	77347	77534	311-335	1.000000	54.0
petD-rpoA_p2	petD-rpoA	2	TGGTTGGGTATTGGAGCAAC	GCATTTCGTAATGGATTTACCGA	20	23	50.0	39.1	58.1	57.6	0.0	15.4	3.494654	77347	77534	312-336	1.000000	54.0
petD-rpoA_p3	petD-rpoA	3	GGTTGGGTATTGGAGCAACA	AGCATTTCGTAATGGATTTACCG	20	23	50.0	39.1	58.1	57.6	0.0	15.4	3.500438	77347	77534	312-336	1.000000	54.0
petD-rpoA_p4	petD-rpoA	4	TGGTTGGGTATTGGAGCAAC	AGCATTTCGTAATGGATTTACCG	20	23	50.0	39.1	58.1	57.6	0.0	15.4	3.500438	77347	77534	313-337	1.000000	54.0
petD-rpoA_p5	petD-rpoA	5	GGTTGGGTATTGGAGCAACA	GCATTTCGTAATGGATTTACCG	20	22	50.0	40.9	58.1	56.2	0.0	15.4	3.868546	77347	77534	311-335	1.000000	51.0
ndhF-rpl32_p1	ndhF-rpl32	1	AACAGGAAGTGGAGCGAAAG	AGTACGTTTCTTCGGAACCG	20	20	50.0	50.0	58.1	57.9	0.0	6.9	0.171859	110253	110955	781-1136	1.000000	81.0
ndhF-rpl32_p2	ndhF-rpl32	2	GAACAGGAAGTGGAGCGAAA	AGTACGTTTCTTCGGAACCG	20	20	50.0	50.0	58.1	57.9	0.0	6.9	0.177141	110253	110955	782-1137	1.000000	80.9
ndhF-rpl32_p3	ndhF-rpl32	3	CCGTCGGAATAAGGAGAAGC	AGTACGTTTCTTCGGAACCG	20	20	55.0	50.0	58.1	57.9	4.9	6.9	0.201868	110253	110955	821-1176	1.000000	80.6
ndhF-rpl32_p4	ndhF-rpl32	4	CGTCGGAATAAGGAGAAGCC	AGTACGTTTCTTCGGAACCG	20	20	55.0	50.0	58.1	57.9	0.0	6.9	0.201868	110253	110955	820-1175	1.000000	80.6
ndhF-rpl32_p5	ndhF-rpl32	5	GCCGTCGGAATAAGGAGAAG	AGTACGTTTCTTCGGAACCG	20	20	55.0	50.0	58.1	57.9	17.5	6.9	0.201868	110253	110955	822-1177	1.000000	80.6
rps15-ycf1_p1	rps15-ycf1	1	AATGTGCAGTAAGTCTCCGT	GGCTACATATGTATCCGCGA	20	20	45.0	50.0	56.9	57.1	0.0	17.6	2.064030	120729	121179	604-608	1.000000	65.6
rps15-ycf1_p2	rps15-ycf1	2	AATGTGCAGTAAGTCTCCGT	AGGCTACATATGTATCCGCG	20	20	45.0	50.0	56.9	57.1	0.0	23.5	2.071988	120729	121179	605-609	1.000000	65.5
rps15-ycf1_p3	rps15-ycf1	3	AAATGTGCAGTAAGTCTCCGT	GGCTACATATGTATCCGCGA	21	20	42.9	50.0	57.6	57.1	0.0	17.6	2.336123	120729	121179	605-609	1.000000	63.4
rps15-ycf1_p4	rps15-ycf1	4	AAATGTGCAGTAAGTCTCCGT	AGGCTACATATGTATCCGCG	21	20	42.9	50.0	57.6	57.1	0.0	23.5	2.344080	120729	121179	606-610	1.000000	63.4
rps15-ycf1_p5	rps15-ycf1	5	AATGTGCAGTAAGTCTCCGT	AAGGCTACATATGTATCCGCG	20	21	45.0	47.6	56.9	57.8	0.0	23.5	2.362747	120729	121179	606-610	1.000000	63.2