primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	ACCAATTTCGCCATATCCCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137364	13943	14785	893-1135	1.000000	80.4
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	13943	14785	901-1143	1.000000	79.8
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	ACCAATTTCGCCATATCCCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.0	57.8	0.0	15.5	0.245117	13943	14785	925-1167	1.000000	79.5
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.1	57.8	0.0	15.5	0.309926	13943	14785	933-1175	1.000000	78.9
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	CCCATTGTGAATTCCAGGGT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	57.8	57.9	2.3	0.0	0.369229	13943	14785	986-1228	1.000000	78.4
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	GAGAGATGGCTGAGTGGTTG	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.0	58.4	0.0	3.5	0.443887	25508	25705	396-397	1.000000	78.3
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	GCTGAGTGGTTGATAGCTCC	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.1	58.4	0.0	3.5	0.475787	25508	25705	388-389	1.000000	78.0
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	GGCTGAGTGGTTGATAGCTC	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.1	58.4	0.0	3.5	0.475787	25508	25705	389-390	1.000000	78.0
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	GGTTGATAGCTCCGGTCTTG	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.1	58.4	0.0	3.5	0.481207	25508	25705	381-382	1.000000	78.0
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	CTGAGTGGTTGATAGCTCCG	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	57.8	58.4	0.0	3.5	0.638439	25508	25705	387-388	1.000000	76.7
atpH-atpF_p1	atpH-atpF	1	AGCAGAGGACAAAATACGGG	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	57.6	57.9	0.0	13.0	0.517112	47033	47604	734-736	1.000000	77.6
atpH-atpF_p2	atpH-atpF	2	GCAGAGGACAAAATACGGGG	GATGGCCAGTAACTCAAGCA	20	20	55.0	50.0	58.6	57.9	0.0	13.0	0.734334	47033	47604	733-735	1.000000	75.9
atpH-atpF_p3	atpH-atpF	3	AGCAGAGGACAAAATACGGG	CGGATGGCCAGTAACTCAAG	20	20	50.0	55.0	57.6	58.3	0.0	13.0	0.746599	47033	47604	736-738	1.000000	75.8
atpH-atpF_p4	atpH-atpF	4	AGCAGAGGACAAAATACGGG	TGGCCAGTAACTCAAGCAAA	20	20	50.0	45.0	57.6	57.6	0.0	0.0	0.759916	47033	47604	732-734	1.000000	75.7
atpH-atpF_p5	atpH-atpF	5	AGCAGAGGACAAAATACGGG	ATCAAACCCGAAACTTCCGG	20	20	50.0	50.0	57.6	58.5	0.0	0.0	0.872928	47033	47604	756-758	1.000000	74.7
trnG-UCC-trnS-GCU_p1	trnG-UCC-trnS-GCU	1	AGGGTCCCTAGAACGAATCA	CAATCCGACGCTTTAGTCCA	20	20	50.0	50.0	57.5	58.0	9.4	0.0	0.549426	51558	52319	831-843	1.000000	82.3
trnG-UCC-trnS-GCU_p2	trnG-UCC-trnS-GCU	2	AGGGTCCCTAGAACGAATCA	AATCCGACGCTTTAGTCCAC	20	20	50.0	50.0	57.5	58.0	9.4	0.0	0.550814	51558	52319	830-842	1.000000	82.3
trnG-UCC-trnS-GCU_p3	trnG-UCC-trnS-GCU	3	GGGTCCCTAGAACGAATCAC	CAATCCGACGCTTTAGTCCA	20	20	55.0	50.0	57.4	58.0	0.0	0.0	0.603268	51558	52319	830-842	1.000000	81.8
trnG-UCC-trnS-GCU_p4	trnG-UCC-trnS-GCU	4	GGGTCCCTAGAACGAATCAC	AATCCGACGCTTTAGTCCAC	20	20	55.0	50.0	57.4	58.0	0.0	0.0	0.604656	51558	52319	829-841	1.000000	81.8
trnG-UCC-trnS-GCU_p5	trnG-UCC-trnS-GCU	5	AGGGTCCCTAGAACGAATCA	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.5	58.2	9.4	0.0	0.696192	51558	52319	884-896	1.000000	80.8
psbK-trnQ-UUG_p1	psbK-trnQ-UUG	1	AGCTTCAGGTAATTTCGCCA	GACCAAAACCCGTTGCCTTA	20	20	45.0	50.0	57.5	58.7	0.0	0.0	1.169432	53299	53954	781-794	1.000000	71.7
psbK-trnQ-UUG_p2	psbK-trnQ-UUG	2	AGCTTCAGGTAATTTCGCCA	CGAATAACGGGACCAAAACC	20	20	45.0	50.0	57.5	57.1	0.0	0.0	1.395898	53299	53954	791-804	1.000000	69.8
psbK-trnQ-UUG_p3	psbK-trnQ-UUG	3	AGCTTCAGGTAATTTCGCCA	ACCAAAACCCGTTGCCTTAC	20	20	45.0	50.0	57.5	59.0	0.0	0.0	1.455582	53299	53954	780-793	1.000000	69.4
psbK-trnQ-UUG_p4	psbK-trnQ-UUG	4	AGCTTCAGGTAATTTCGCCA	GAATAACGGGACCAAAACCC	20	20	45.0	50.0	57.5	56.7	0.0	0.0	1.800119	53299	53954	790-803	1.000000	66.6
psbK-trnQ-UUG_p5	psbK-trnQ-UUG	5	GCTTCAGGTAATTTCGCCAAG	GACCAAAACCCGTTGCCTTA	21	20	47.6	50.0	57.9	58.7	0.0	0.0	1.816301	53299	53954	780-793	1.000000	66.5
trnQ-UUG-accD_p1	trnQ-UUG-accD	1	GAGGTTCGAATCCTTCCGTC	AGCATTGAGTTAAACCACCGT	20	21	55.0	42.9	58.1	58.1	16.9	0.0	1.217356	54027	54507	534-549	1.000000	72.0
trnQ-UUG-accD_p2	trnQ-UUG-accD	2	GAGGTTCGAATCCTTCCGTC	GCATTGAGTTAAACCACCGT	20	20	55.0	45.0	58.1	56.6	16.9	0.0	1.440816	54027	54507	533-548	1.000000	70.3
trnQ-UUG-accD_p3	trnQ-UUG-accD	3	GAGGTTCGAATCCTTCCGTC	GAGCATTGAGTTAAACCACCG	20	21	55.0	47.6	58.1	57.5	16.9	0.0	1.547596	54027	54507	535-550	1.000000	69.4
trnQ-UUG-accD_p4	trnQ-UUG-accD	4	GAGGTTCGAATCCTTCCGTC	GCATTGAGTTAAACCACCGTT	20	21	55.0	42.9	58.1	57.4	16.9	0.0	1.712383	54027	54507	533-548	1.000000	68.1
trnQ-UUG-accD_p5	trnQ-UUG-accD	5	GAGGTTCGAATCCTTCCGTC	AGCATTGAGTTAAACCACCG	20	20	55.0	45.0	58.1	56.3	16.9	0.0	1.735759	54027	54507	534-549	1.000000	67.9
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	CGGTGCCACAGAGAGATATG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.245712	63093	63608	607-640	1.000000	81.9
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	CGGTGCCACAGAGAGATATG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.247056	63093	63608	606-639	1.000000	81.9
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACCCGTGACATTTTGTACCC	CGAAGCATAGAGTACTGGCC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.241290	63093	63608	629-662	1.000000	81.8
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CCCGTGACATTTTGTACCCA	CGAAGCATAGAGTACTGGCC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.242633	63093	63608	628-661	1.000000	81.8
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	ACAGGATTTGAACCCGTGAC	CGAAGCATAGAGTACTGGCC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.249326	63093	63608	640-673	1.000000	81.7
clpP-psbB_p1	clpP-psbB	1	CTACCCGAGGAATACCGACT	TGGACAGAAAGTAAACGGCC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.134659	67394	67856	556-1114	1.000000	85.8
clpP-psbB_p2	clpP-psbB	2	CTACCCGAGGAATACCGACT	AAACGGCCGGGATCATTTAA	20	20	55.0	45.0	58.0	57.9	0.0	31.8	0.153321	67394	67856	544-1102	1.000000	85.7
clpP-psbB_p3	clpP-psbB	3	ATGCATCCTCATCTCCAGGA	TGGACAGAAAGTAAACGGCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.226921	67394	67856	586-1144	1.000000	84.8
clpP-psbB_p4	clpP-psbB	4	TGCATCCTCATCTCCAGGAA	TGGACAGAAAGTAAACGGCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.229436	67394	67856	585-1143	1.000000	84.8
clpP-psbB_p5	clpP-psbB	5	ATGCATCCTCATCTCCAGGA	AAACGGCCGGGATCATTTAA	20	20	50.0	45.0	57.9	57.9	0.0	31.8	0.245583	67394	67856	574-1132	1.000000	84.8
psbT-psbN_p1	psbT-psbN	1	AGAACCCCCTATAGTTCCAACT	CCATTCGAAGAACATGGGGA	22	20	45.5	50.0	57.9	57.6	0.0	3.3	2.508395	69687	69751	126-126	1.000000	64.4
psbT-psbN_p2	psbT-psbN	2	AGAGAACCCCCTATAGTTCCA	CCATTCGAAGAACATGGGGA	21	20	47.6	50.0	56.9	57.6	0.0	3.3	2.503274	69687	69751	128-128	1.000000	64.3
psbT-psbN_p3	psbT-psbN	3	AGAGAACCCCCTATAGTTCCA	TCCATTCGAAGAACATGGGG	21	20	47.6	50.0	56.9	57.6	0.0	13.4	2.503274	69687	69751	129-129	1.000000	64.3
psbT-psbN_p4	psbT-psbN	4	AGAACCCCCTATAGTTCCAACT	TCCATTCGAAGAACATGGGG	22	20	45.5	50.0	57.9	57.6	0.0	13.4	2.508395	69687	69751	127-127	1.000000	64.3
psbT-psbN_p5	psbT-psbN	5	AGAGAACCCCCTATAGTTCCA	GGCAACCCTCTCAACAACTA	21	20	47.6	50.0	56.9	57.4	0.0	0.0	2.646384	69687	69751	154-154	1.000000	62.5
rps8-rpl14_p1	rps8-rpl14	1	ACTCATGTCAGCATTGCGTA	AATAGCCCGCGAATTGAGAC	20	20	45.0	50.0	57.6	58.4	2.4	8.2	0.801960	75853	76570	838-842	1.000000	76.1
rps8-rpl14_p2	rps8-rpl14	2	TACTCATGTCAGCATTGCGT	AATAGCCCGCGAATTGAGAC	20	20	45.0	50.0	57.6	58.4	2.4	8.2	0.801960	75853	76570	839-843	1.000000	76.1
rps8-rpl14_p3	rps8-rpl14	3	ACTCATGTCAGCATTGCGTA	CAATAGCCCGCGAATTGAGA	20	20	45.0	50.0	57.6	58.4	2.4	8.2	0.803080	75853	76570	839-843	1.000000	76.0
rps8-rpl14_p4	rps8-rpl14	4	TACTCATGTCAGCATTGCGT	CAATAGCCCGCGAATTGAGA	20	20	45.0	50.0	57.6	58.4	2.4	8.2	0.803080	75853	76570	840-844	1.000000	76.0
rps8-rpl14_p5	rps8-rpl14	5	TCAGCATTGCGTATCAAGGT	AATAGCCCGCGAATTGAGAC	20	20	45.0	50.0	57.6	58.4	2.4	8.2	0.818529	75853	76570	831-835	1.000000	75.9
rpl14-rpl16_p1	rpl14-rpl16	1	ACCGTCGATTACTAGCTCCT	AGCAGCATCCAAAATGCCTA	20	20	50.0	45.0	57.7	57.9	0.0	6.4	0.493570	76940	77058	261-262	1.000000	77.6
rpl14-rpl16_p2	rpl14-rpl16	2	ACCGTCGATTACTAGCTCCT	TAGCAGCATCCAAAATGCCT	20	20	50.0	45.0	57.7	57.9	0.0	6.4	0.493570	76940	77058	262-263	1.000000	77.6
rpl14-rpl16_p3	rpl14-rpl16	3	ACCGTCGATTACTAGCTCCT	GGGGTCCCGGAAAATATAGC	20	20	50.0	55.0	57.7	57.8	0.0	0.7	0.540866	76940	77058	299-300	1.000000	77.0
rpl14-rpl16_p4	rpl14-rpl16	4	ACCGTCGATTACTAGCTCCT	ACTTTATGAAATGGGCGGGG	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.569739	76940	77058	315-316	1.000000	76.7
rpl14-rpl16_p5	rpl14-rpl16	5	ACCGTCGATTACTAGCTCCT	CTTTATGAAATGGGCGGGGT	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.569739	76940	77058	314-315	1.000000	76.7
rps19-rpl2_p1	rps19-rpl2	1	CCGGAAGTTTCGAGTAGGTG	AGATGAAGGAACGCGAAGAC	20	20	55.0	50.0	58.0	58.0	4.7	15.9	0.010161	79917	81160	2154-2168	1.000000	85.8
rps19-rpl2_p2	rps19-rpl2	2	CCGGAAGTTTCGAGTAGGTG	CAGATGAAGGAACGCGAAGA	20	20	55.0	50.0	58.0	58.0	4.7	15.9	0.011539	79917	81160	2155-2169	1.000000	85.8
rps19-rpl2_p3	rps19-rpl2	3	CCGGAAGTTTCGAGTAGGTG	TAAGCCAGATGAAGGAACGC	20	20	55.0	50.0	58.0	58.0	4.7	0.0	0.032006	79917	81160	2160-2174	1.000000	85.7
rps19-rpl2_p4	rps19-rpl2	4	CCGGAAGTTTCGAGTAGGTG	ACGCGAAGACCTAGGATGTA	20	20	55.0	50.0	58.0	58.0	4.7	18.0	0.056274	79917	81160	2144-2158	1.000000	85.5
rps19-rpl2_p5	rps19-rpl2	5	TGGCCTACCATACGATCTGT	AGATGAAGGAACGCGAAGAC	20	20	50.0	50.0	57.9	58.0	3.3	15.9	0.084471	79917	81160	2117-2131	1.000000	85.2
rpl23-trnI-CAU_p1	rpl23-trnI-CAU	1	CCTAGTTGATCCTGATTCGACA	ATTGGCGAAGTCGTAGGTTC	22	20	45.5	50.0	57.3	58.0	0.0	2.3	2.661733	83002	83933	879-1065	1.000000	65.7
rpl23-trnI-CAU_p2	rpl23-trnI-CAU	2	TGTCCTAGTTGATCCTGATTCG	ATTGGCGAAGTCGTAGGTTC	22	20	45.5	50.0	57.3	58.0	0.0	2.3	2.661733	83002	83933	882-1068	1.000000	65.6
rpl23-trnI-CAU_p3	rpl23-trnI-CAU	3	CCTAGTTGATCCTGATTCGACA	CATCCATGGCTGAACGGTTA	22	20	45.5	50.0	57.3	58.0	0.0	10.2	2.692720	83002	83933	915-1101	1.000000	65.3
rpl23-trnI-CAU_p4	rpl23-trnI-CAU	4	TGTCCTAGTTGATCCTGATTCG	CATCCATGGCTGAACGGTTA	22	20	45.5	50.0	57.3	58.0	0.0	10.2	2.692720	83002	83933	918-1104	1.000000	65.3
rpl23-trnI-CAU_p5	rpl23-trnI-CAU	5	CCTAGTTGATCCTGATTCGACA	GTGTAAGCATCCATGGCTGA	22	20	45.5	50.0	57.3	58.0	0.0	19.8	2.698448	83002	83933	922-1108	1.000000	65.2
ycf1_p1	ycf1	1	GAGCTACTGAGGAACAACGG	CGGCTACTTTCTTCTCGGTC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.091643	104766	104885	675-761	1.000000	82.9
ycf1_p2	ycf1	2	GAGCTACTGAGGAACAACGG	CCAGTAGTTGCGGCTACTTT	20	20	55.0	50.0	58.0	57.9	0.0	21.3	0.097093	104766	104885	685-771	1.000000	82.8
ycf1_p3	ycf1	3	GAGCTACTGAGGAACAACGG	TTGGCAAAAGAAGGGGGTTT	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.115909	104766	104885	954-1040	1.000000	82.0
ycf1_p4	ycf1	4	ATCCCGAACCTACACCTAGG	CGGCTACTTTCTTCTCGGTC	20	20	55.0	55.0	57.9	58.1	7.4	0.0	0.172941	104766	104885	882-968	1.000000	81.5
ycf1_p5	ycf1	5	ATCCCGAACCTACACCTAGG	CCAGTAGTTGCGGCTACTTT	20	20	55.0	50.0	57.9	57.9	7.4	21.3	0.178390	104766	104885	892-978	1.000000	81.4
ycf1-rps15_p1	ycf1-rps15	1	ACCCATAATGGAAGCCGTTT	TGAAATTCAACGGATCCGGG	20	20	45.0	50.0	57.5	58.3	0.0	35.9	0.779873	109860	110240	533-562	1.000000	77.0
ycf1-rps15_p2	ycf1-rps15	2	ACCCATAATGGAAGCCGTTT	CCGGGGTTTTCTTCTTCCTT	20	20	45.0	50.0	57.5	57.7	0.0	0.0	0.813082	109860	110240	518-547	1.000000	76.8
ycf1-rps15_p3	ycf1-rps15	3	ACCCATAATGGAAGCCGTTT	ATCCGGGGTTTTCTTCTTCC	20	20	45.0	50.0	57.5	57.5	0.0	0.0	1.034271	109860	110240	520-549	1.000000	75.0
ycf1-rps15_p4	ycf1-rps15	4	ACCCATAATGGAAGCCGTTT	GGATCCGGGGTTTTCTTCTT	20	20	45.0	50.0	57.5	57.5	0.0	0.0	1.034271	109860	110240	522-551	1.000000	75.0
ycf1-rps15_p5	ycf1-rps15	5	ACCCATAATGGAAGCCGTTT	TCCGGGGTTTTCTTCTTCCT	20	20	45.0	50.0	57.5	58.6	0.0	0.0	1.083410	109860	110240	519-548	1.000000	74.7
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	TCCTAAGAGCAGCGTGTCTA	TGGACAGCATTGAAAGCCTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124629	119738	120395	750-775	1.000000	82.2
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	TCCTAAGAGCAGCGTGTCTA	TTGGACAGCATTGAAAGCCT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124629	119738	120395	751-776	1.000000	82.2
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCAGCGTGTCTACCAATTT	TGGACAGCATTGAAAGCCTT	20	20	45.0	45.0	57.8	58.0	0.0	0.0	0.185356	119738	120395	743-768	1.000000	81.8
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	AGCAGCGTGTCTACCAATTT	TTGGACAGCATTGAAAGCCT	20	20	45.0	45.0	57.8	58.0	0.0	0.0	0.185356	119738	120395	744-769	1.000000	81.8
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	CTAAGAGCAGCGTGTCTACC	TGGACAGCATTGAAAGCCTT	20	20	55.0	45.0	57.8	58.0	0.0	0.0	0.196647	119738	120395	748-773	1.000000	81.7