primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psaE_p1	psaE	1	CAAGCAGAAGGTCACAGGTT	ACAAGCTTGTCCTGGGTTTT	20	20	50.0	45.0	58.0	57.8	0.0	26.4	0.186307	19831	20028	1109-1158	1.000000	90.1
psaE_p2	psaE	2	GCAGAAGGTCACAGGTTCAA	ACAAGCTTGTCCTGGGTTTT	20	20	50.0	45.0	58.0	57.8	0.0	26.4	0.191861	19831	20028	1106-1155	1.000000	90.1
psaE_p3	psaE	3	TCGTCTAACGGATCAGGACA	ACAAGCTTGTCCTGGGTTTT	20	20	50.0	45.0	57.9	57.8	0.0	26.4	0.277492	19831	20028	1051-1100	1.000000	89.6
psaE_p4	psaE	4	AACTCAGCGGTAGAGTGTCT	ACAAGCTTGTCCTGGGTTTT	20	20	50.0	45.0	58.1	57.8	16.6	26.4	0.246762	19831	20028	1135-1184	1.000000	89.5
psaE_p5	psaE	5	GAGTGTCTGCCTTACAAGCA	ACAAGCTTGTCCTGGGTTTT	20	20	50.0	45.0	57.8	57.8	0.0	26.4	0.329012	19831	20028	1123-1172	1.000000	88.9
ycf45-ffs_p1	ycf45-ffs	1	TACTCAGCCTTTCAGCTTGC	TCCACTGCTCTACCAACTGA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.196168	22856	23576	1477-1477	0.500000	58.4
ycf45-ffs_p2	ycf45-ffs	2	AGAAGCTAAACGGAGAGGGA	TCCACTGCTCTACCAACTGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.242987	22856	23576	1526-1526	0.500000	58.1
ycf45-ffs_p3	ycf45-ffs	3	TACTCAGCCTTTCAGCTTGC	CATCGTAATGGTCCGCTAGG	20	20	50.0	55.0	58.2	57.9	0.0	0.0	0.252479	22856	23576	1601-1601	0.500000	58.0
ycf45-ffs_p4	ycf45-ffs	4	AGAAGCTAAACGGAGAGGGA	CATCGTAATGGTCCGCTAGG	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.299298	22856	23576	1650-1650	0.500000	57.6
ycf45-ffs_p5	ycf45-ffs	5	GAAGCTAAACGGAGAGGGAC	TCCACTGCTCTACCAACTGA	20	20	55.0	50.0	57.7	58.0	0.0	0.0	0.306512	22856	23576	1525-1525	0.500000	57.5
dnaB_p1	dnaB	1	TCAGTTGGTAGAGCAGTGGA	GGACTTGAACCCTCGACATC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.021222	23848	25209	1571-1577	1.000000	87.6
dnaB_p2	dnaB	2	TCAGTTGGTAGAGCAGTGGA	GAAGGACTTGAACCCTCGAC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.091407	23848	25209	1574-1580	1.000000	87.0
dnaB_p3	dnaB	3	TCAGTTGGTAGAGCAGTGGA	CTGAAGGACTTGAACCCTCG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.095736	23848	25209	1576-1582	1.000000	87.0
dnaB_p4	dnaB	4	ACGAAACCCAATCAACGACA	GGACTTGAACCCTCGACATC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.055243	23848	25209	2150-2150	0.500000	59.6
dnaB_p5	dnaB	5	TCAGTTGGTAGAGCAGTGGA	AGGTGCAGGTGTAGCTTTTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.112122	23848	25209	1843-1843	0.500000	59.1
petA-chlI_p1	petA-chlI	1	AGCAGAGCAACCGTTAAACA	GCAAAAGCACATGCAGCATA	20	20	45.0	45.0	58.0	58.0	0.0	7.8	0.039158	44542	45575	1398-1398	0.500000	59.7
petA-chlI_p2	petA-chlI	2	AGCAGAGCAACCGTTAAACA	GGTATGCATGCCGAAATTCG	20	20	45.0	50.0	58.0	58.0	0.0	18.6	0.074763	44542	45575	1662-1662	0.500000	59.4
petA-chlI_p3	petA-chlI	3	AGCAGAGCAACCGTTAAACA	CAGCATACAATGGTCGGGAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.075164	44542	45575	1385-1385	0.500000	59.4
petA-chlI_p4	petA-chlI	4	AGCAGAGCAACCGTTAAACA	GATCGCTTTGGTATGCATGC	20	20	45.0	50.0	58.0	58.0	0.0	11.8	0.080310	44542	45575	1671-1671	0.500000	59.4
petA-chlI_p5	petA-chlI	5	ACTCAAGCAGGGCAAGTTAC	GCAAAAGCACATGCAGCATA	20	20	50.0	45.0	58.1	58.0	0.0	7.8	0.103392	44542	45575	1541-1541	0.500000	59.2
psaM-rps14_p1	psaM-rps14	1	GCGACATAAACTTGAAAATCAGT	AGGGGTTACTAAATCAAGTTGGT	23	23	34.8	39.1	56.1	57.4	0.0	2.3	8.484493	46772	46908	183-191	1.000000	50.3
psaM-rps14_p2	psaM-rps14	2	GCGACATAAACTTGAAAATCAGT	CAGGGGTTACTAAATCAAGTTGG	23	23	34.8	43.5	56.1	57.1	0.0	2.3	8.787671	46772	46908	184-192	1.000000	50.2
psaM-rps14_p3	psaM-rps14	3	GCGACATAAACTTGAAAATCAGT	CCAGGGGTTACTAAATCAAGTTG	23	23	34.8	43.5	56.1	57.1	0.0	0.0	8.787671	46772	46908	185-193	1.000000	50.2
psaM-rps14_p4	psaM-rps14	4	GCGACATAAACTTGAAAATCAGT	ACCAGGGGTTACTAAATCAAGT	23	22	34.8	40.9	56.1	56.7	0.0	0.0	8.176688	46772	46908	186-194	1.000000	50.1
psaM-rps14_p5	psaM-rps14	5	GCGACATAAACTTGAAAATCAGT	ACCAGGGGTTACTAAATCAAGTT	23	23	34.8	39.1	56.1	57.4	0.0	0.0	8.484493	46772	46908	186-194	1.000000	50.1
trnY-GUA-petG_p1	trnY-GUA-petG	1	GTAAATCCGCTGCGTTATGC	TCGTCGTGGAAATCAATTTGA	20	21	50.0	38.1	58.0	56.2	21.6	0.8	2.819373	51174	51382	278-286	1.000000	67.7
trnY-GUA-petG_p2	trnY-GUA-petG	2	AAATCCGCTGCGTTATGCTA	TCGTCGTGGAAATCAATTTGA	20	21	45.0	38.1	58.1	56.2	21.6	0.8	2.852551	51174	51382	276-284	1.000000	67.5
trnY-GUA-petG_p3	trnY-GUA-petG	3	TAAATCCGCTGCGTTATGCT	TCGTCGTGGAAATCAATTTGA	20	21	45.0	38.1	58.1	56.2	21.6	0.8	2.852551	51174	51382	277-285	1.000000	67.5
trnY-GUA-petG_p4	trnY-GUA-petG	4	AAGGGGGCGGATTGTAAATC	TCGTCGTGGAAATCAATTTGA	20	21	50.0	38.1	57.9	56.2	0.0	0.8	2.868021	51174	51382	291-299	1.000000	67.2
trnY-GUA-petG_p5	trnY-GUA-petG	5	TGCGTTATGCTACGTTGGTT	TCGTCGTGGAAATCAATTTGA	20	21	45.0	38.1	58.2	56.2	0.0	0.8	3.002372	51174	51382	268-276	1.000000	66.3
ycf90_p1	ycf90	1	TACACAAATGGGTGAACCGG	TTAGTAGGTGGTTTCGCAGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.190048	58449	59870	2107-2108	1.000000	86.7
ycf90_p2	ycf90	2	TACACAAATGGGTGAACCGG	GGTGGTTTCGCAGCAATAAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.123910	58449	59870	2101-2101	0.500000	59.0
ycf90_p3	ycf90	3	ATGGCACCTACACAAATGGG	GGTGGTTTCGCAGCAATAAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.171508	58449	59870	2109-2109	0.500000	58.6
ycf90_p4	ycf90	4	TACACAAATGGGTGAACCGG	TCGTTGGTTAGCAGTCCATG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.194404	58449	59870	2407-2407	0.500000	58.4
ycf90_p5	ycf90	5	TACACAAATGGGTGAACCGG	AGGTGGTTTCGCAGCAATAA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.196811	58449	59870	2102-2102	0.500000	58.4
ycf89-rns_p1	ycf89-rns	1	TGGTATTCAGAGCGTCCTGA	CATTGCGGAAAATTCCCCAC	20	20	50.0	50.0	58.2	58.0	5.1	6.6	0.161423	67209	67771	1087-1087	0.500000	58.7
ycf89-rns_p2	ycf89-rns	2	TGGTATTCAGAGCGTCCTGA	TCTCGTACGACTTGCATGTG	20	20	50.0	50.0	58.2	58.0	5.1	15.3	0.175483	67209	67771	805-805	0.500000	58.6
ycf89-rns_p3	ycf89-rns	3	TGGTATTCAGAGCGTCCTGA	TATTGCTCCGTCAGGCTTTC	20	20	50.0	50.0	58.2	58.0	5.1	0.0	0.176847	67209	67771	1110-1110	0.500000	58.6
ycf89-rns_p4	ycf89-rns	4	TGGTATTCAGAGCGTCCTGA	CAACCCATAGGCAGTCTTCC	20	20	50.0	55.0	58.2	58.0	5.1	0.0	0.196794	67209	67771	1139-1139	0.500000	58.4
ycf89-rns_p5	ycf89-rns	5	TGGTATTCAGAGCGTCCTGA	CTTGCATCCTCCGTCTTACC	20	20	50.0	55.0	58.2	58.1	5.1	0.0	0.211347	67209	67771	1238-1238	0.500000	58.3
rns-trnI-GAU_p1	rns-trnI-GAU	1	GACTGGGGTGAAGTCGTAAC	TTATCAGGGGTGCGCTCTAA	20	20	55.0	50.0	57.9	58.5	0.0	9.7	0.584311	69264	69322	158-159	1.000000	78.9
rns-trnI-GAU_p2	rns-trnI-GAU	2	GGAGGGTGCCTAAGGTAGAA	TTATCAGGGGTGCGCTCTAA	20	20	55.0	50.0	58.1	58.5	0.0	9.7	0.632844	69264	69322	183-184	1.000000	78.0
rns-trnI-GAU_p3	rns-trnI-GAU	3	GGGGTGAAGTCGTAACAAGG	TTATCAGGGGTGCGCTCTAA	20	20	55.0	50.0	58.2	58.5	0.0	9.7	0.703731	69264	69322	154-155	1.000000	78.0
rns-trnI-GAU_p4	rns-trnI-GAU	4	GACTGGGGTGAAGTCGTAAC	TGGGCCATTCTGGATTTGAA	20	20	55.0	45.0	57.9	57.4	0.0	0.0	0.695676	69264	69322	193-194	1.000000	77.3
rns-trnI-GAU_p5	rns-trnI-GAU	5	TCTAACCATTCGGAGGAGGG	TTATCAGGGGTGCGCTCTAA	20	20	55.0	50.0	58.2	58.5	0.0	9.7	0.718809	69264	69322	197-198	1.000000	77.1
rps4_p1	rps4	1	CTACCATCTCGTCGGGTAGT	GTTAAGGCAGTGGGTTGTGA	20	20	55.0	50.0	58.0	58.0	14.1	0.0	0.059788	84557	85174	903-903	0.500000	59.5
rps4_p2	rps4	2	CTACCATCTCGTCGGGTAGT	GTGGGTTGTGATTCCACCAT	20	20	55.0	50.0	58.0	58.1	14.1	0.0	0.112092	84557	85174	894-894	0.500000	59.1
rps4_p3	rps4	3	CGTCGGGTAGTGTTTTCCAT	GTTAAGGCAGTGGGTTGTGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.113290	84557	85174	894-894	0.500000	59.1
rps4_p4	rps4	4	CGTCGGGTAGTGTTTTCCAT	GTGGGTTGTGATTCCACCAT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.165594	84557	85174	885-885	0.500000	58.7
rps4_p5	rps4	5	CTACCATCTCGTCGGGTAGT	CACTCGCCCTACTTCAAACA	20	20	55.0	50.0	58.0	57.8	14.1	0.0	0.199265	84557	85174	855-855	0.500000	58.4
thiS-psb28_p1	thiS-psb28	1	TCACAACCCACTGCCTTAAC	CTTTGACTCCGCCATTCGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028923	85617	86013	1206-1922	1.000000	87.9
thiS-psb28_p2	thiS-psb28	2	TCACAACCCACTGCCTTAAC	TTTGACTCCGCCATTCGTAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028923	85617	86013	1205-1921	1.000000	87.9
thiS-psb28_p3	thiS-psb28	3	TCACAACCCACTGCCTTAAC	AGGGATGGAAGTACTGGGAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.143400	85617	86013	1007-1007	0.500000	58.9
thiS-psb28_p4	thiS-psb28	4	ACCAGTTACACCGTTTCGTC	CTTTGACTCCGCCATTCGTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.147781	85617	86013	1566-1566	0.500000	58.8
thiS-psb28_p5	thiS-psb28	5	ACCAGTTACACCGTTTCGTC	TTTGACTCCGCCATTCGTAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.147781	85617	86013	1565-1565	0.500000	58.8
trnQ-UUG-trnR-ACG_p1	trnQ-UUG-trnR-ACG	1	AAAGTCCACAGCCTTACCAC	GGGGTTCGAATCCCTTCTTG	20	20	50.0	55.0	57.7	58.2	0.0	35.1	0.520507	86517	86584	128-128	1.000000	81.5
trnQ-UUG-trnR-ACG_p2	trnQ-UUG-trnR-ACG	2	CAAAGTCCACAGCCTTACCA	GGGGTTCGAATCCCTTCTTG	20	20	50.0	55.0	57.7	58.2	0.0	35.1	0.518915	86517	86584	129-129	1.000000	81.4
trnQ-UUG-trnR-ACG_p3	trnQ-UUG-trnR-ACG	3	GAGTCAAAGTCCACAGCCTT	GGGGTTCGAATCCCTTCTTG	20	20	50.0	55.0	57.7	58.2	0.0	35.1	0.510986	86517	86584	133-133	1.000000	81.3
trnQ-UUG-trnR-ACG_p4	trnQ-UUG-trnR-ACG	4	TTAAAAACTGGGGTGGCAGG	GGGGTTCGAATCCCTTCTTG	20	20	50.0	55.0	58.3	58.2	0.0	35.1	0.537747	86517	86584	173-173	1.000000	79.9
trnQ-UUG-trnR-ACG_p5	trnQ-UUG-trnR-ACG	5	GTGGCAGGATTCGAACCTAC	GGGGTTCGAATCCCTTCTTG	20	20	55.0	55.0	58.3	58.2	15.9	35.1	0.595411	86517	86584	161-161	1.000000	79.7
groEL-dnaK_p1	groEL-dnaK	1	CTGTCATTCGTGTAGGTGCA	GCAGGTATTTTAGCGGGTGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.111529	88417	88900	1629-1629	0.500000	59.1
groEL-dnaK_p2	groEL-dnaK	2	TGCTGTCATTCGTGTAGGTG	GCAGGTATTTTAGCGGGTGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.111529	88417	88900	1631-1631	0.500000	59.1
groEL-dnaK_p3	groEL-dnaK	3	TGTCATTCGTGTAGGTGCAG	GCAGGTATTTTAGCGGGTGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.111529	88417	88900	1628-1628	0.500000	59.1
groEL-dnaK_p4	groEL-dnaK	4	GTCATTCGTGTAGGTGCAGT	GCAGGTATTTTAGCGGGTGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.112966	88417	88900	1627-1627	0.500000	59.1
groEL-dnaK_p5	groEL-dnaK	5	TGTCATTCGTGTAGGTGCAG	AGCAGGTATTTTAGCGGGTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.117235	88417	88900	1629-1629	0.500000	59.1
rpl2_p1	rpl2	1	GGATCAAGCCGTTCTCCATT	GTCGAACTACGTGACCAAGT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.456543	92654	93481	1719-1719	0.500000	56.3
rpl2_p2	rpl2	2	TAGAGCTCGAGCAGGATCAA	GTCGAACTACGTGACCAAGT	20	20	50.0	50.0	57.9	57.6	36.9	0.0	0.471014	92654	93481	1732-1732	0.500000	56.2
rpl2_p3	rpl2	3	AACACCGCTCATTTACCGAA	GTCGAACTACGTGACCAAGT	20	20	45.0	50.0	57.8	57.6	0.0	0.0	0.585272	92654	93481	1136-1136	0.500000	55.3
rpl2_p4	rpl2	4	ACACCGCTCATTTACCGAAA	GTCGAACTACGTGACCAAGT	20	20	45.0	50.0	57.8	57.6	0.0	0.0	0.585272	92654	93481	1135-1135	0.500000	55.3
rpl2_p5	rpl2	5	ATATCTGGGCTGGAAATCGC	GTCGAACTACGTGACCAAGT	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.603548	92654	93481	1095-1095	0.500000	55.2
thiG_p1	thiG	1	GCACGCTTCTTCCTTACAGA	TGGTCGTAGGTTCAAGTCCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.085656	110167	110976	1322-1343	1.000000	88.9
thiG_p2	thiG	2	CGTCCACTACGTCGTTCAAT	TGGTCGTAGGTTCAAGTCCT	20	20	50.0	50.0	58.0	58.0	1.8	0.0	0.032232	110167	110976	1295-1295	0.500000	59.7
thiG_p3	thiG	3	CGTCCACTACGTCGTTCAAT	ACGAACTAAAAACGTGGGCT	20	20	50.0	45.0	58.0	58.0	1.8	0.0	0.069193	110167	110976	1656-1656	0.500000	59.4
thiG_p4	thiG	4	CGTCCACTACGTCGTTCAAT	TTCAAGTCCTACCTGGGGAG	20	20	50.0	55.0	58.0	58.0	1.8	0.0	0.074240	110167	110976	1285-1285	0.500000	59.4
thiG_p5	thiG	5	AACCAAACGCCTACGGAAAT	TGGTCGTAGGTTCAAGTCCT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.105125	110167	110976	1186-1186	0.500000	59.2
trnI-GAU-rns_p1	trnI-GAU-rns	1	TTATCAGGGGTGCGCTCTAA	GACTGGGGTGAAGTCGTAAC	20	20	50.0	55.0	58.5	57.9	9.7	0.0	0.584311	126437	126495	158-159	1.000000	78.9
trnI-GAU-rns_p2	trnI-GAU-rns	2	TTATCAGGGGTGCGCTCTAA	GGAGGGTGCCTAAGGTAGAA	20	20	50.0	55.0	58.5	58.1	9.7	0.0	0.632844	126437	126495	183-184	1.000000	78.0
trnI-GAU-rns_p3	trnI-GAU-rns	3	TTATCAGGGGTGCGCTCTAA	GGGGTGAAGTCGTAACAAGG	20	20	50.0	55.0	58.5	58.2	9.7	0.0	0.703731	126437	126495	154-155	1.000000	78.0
trnI-GAU-rns_p4	trnI-GAU-rns	4	TGGGCCATTCTGGATTTGAA	GACTGGGGTGAAGTCGTAAC	20	20	45.0	55.0	57.4	57.9	0.0	0.0	0.695676	126437	126495	193-194	1.000000	77.3
trnI-GAU-rns_p5	trnI-GAU-rns	5	TTATCAGGGGTGCGCTCTAA	TCTAACCATTCGGAGGAGGG	20	20	50.0	55.0	58.5	58.2	9.7	0.0	0.718809	126437	126495	197-198	1.000000	77.1
rns-ycf89_p1	rns-ycf89	1	CATTGCGGAAAATTCCCCAC	TGGTATTCAGAGCGTCCTGA	20	20	50.0	50.0	58.0	58.2	6.6	5.1	0.161423	127988	128550	1087-1087	0.500000	58.7
rns-ycf89_p2	rns-ycf89	2	TCTCGTACGACTTGCATGTG	TGGTATTCAGAGCGTCCTGA	20	20	50.0	50.0	58.0	58.2	15.3	5.1	0.175483	127988	128550	805-805	0.500000	58.6
rns-ycf89_p3	rns-ycf89	3	TATTGCTCCGTCAGGCTTTC	TGGTATTCAGAGCGTCCTGA	20	20	50.0	50.0	58.0	58.2	0.0	5.1	0.176847	127988	128550	1110-1110	0.500000	58.6
rns-ycf89_p4	rns-ycf89	4	CAACCCATAGGCAGTCTTCC	TGGTATTCAGAGCGTCCTGA	20	20	55.0	50.0	58.0	58.2	0.0	5.1	0.196794	127988	128550	1139-1139	0.500000	58.4
rns-ycf89_p5	rns-ycf89	5	CTTGCATCCTCCGTCTTACC	TGGTATTCAGAGCGTCCTGA	20	20	55.0	50.0	58.1	58.2	0.0	5.1	0.211347	127988	128550	1238-1238	0.500000	58.3