primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ycf4-cemA	1	GTTTATGCTCGTCGTGTCCT	TTCGGATTGCCCAGTATTCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.059726	2695	3363	913-954	1.000000	87.2
ycf4-cemA_p2	ycf4-cemA	2	AATCGTCGCATATTCCTCCG	TTCGGATTGCCCAGTATTCG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.117888	2695	3363	982-1023	1.000000	86.3
ycf4-cemA_p3	ycf4-cemA	3	CGTCGCATATTCCTCCGATT	TTCGGATTGCCCAGTATTCG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.117888	2695	3363	979-1020	1.000000	86.3
ycf4-cemA_p4	ycf4-cemA	4	AACTTATAGCGGGGTCTCGA	TTCGGATTGCCCAGTATTCG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.114866	2695	3363	1287-1328	1.000000	85.7
ycf4-cemA_p5	ycf4-cemA	5	ACTTATAGCGGGGTCTCGAA	TTCGGATTGCCCAGTATTCG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.114866	2695	3363	1286-1327	1.000000	85.7
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCTTGT	20	21	50.0	47.6	58.2	57.4	5.4	0.0	1.739420	5221	6065	901-929	1.000000	71.9
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCTTGT	20	21	50.0	47.6	58.2	57.4	5.4	0.0	1.739420	5221	6065	903-931	1.000000	71.9
petA-psbJ_p3	petA-psbJ	3	AGAAGGTTCAATTGGCCGAA	CCGGATTAGGTTCATCCTTGT	20	21	45.0	47.6	57.7	57.4	5.4	0.0	1.847001	5221	6065	900-928	1.000000	71.1
petA-psbJ_p4	petA-psbJ	4	CGTGTCCAAGGTCTTTTGGT	CCGGATTAGGTTCATCCTTGT	20	21	50.0	47.6	58.3	57.4	4.8	0.0	1.885084	5221	6065	979-1007	1.000000	70.6
petA-psbJ_p5	petA-psbJ	5	GAGAAGGTTCAATTGGCCGA	GCCGATACTACTGGAAGGAT	20	20	50.0	50.0	58.2	56.2	5.4	0.0	1.972289	5221	6065	998-1026	1.000000	69.9
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	AATAGACCTGCTAAAGCCGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.144828	8589	8871	415-466	1.000000	89.6
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	AATAGACCTGCTAAAGCCGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.146171	8589	8871	414-465	1.000000	89.6
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	AATAGACCTGCTAAAGCCGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.152864	8589	8871	426-477	1.000000	89.5
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	AATAGACCTGCTAAAGCCGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.154197	8589	8871	425-476	1.000000	89.5
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	AATAGACCTGCTAAAGCCGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.154197	8589	8871	428-479	1.000000	89.5
rps12-clpP_p1	rps12-clpP	1	TGACCTTGGTAGTCCGTTCT	TACAAAGGACACGCAAACCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.045188	11748	11905	681-717	1.000000	86.3
rps12-clpP_p2	rps12-clpP	2	TGACCTTGGTAGTCCGTTCT	TAGAAAGCAACGGAGCCATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.029546	11748	11905	1050-1086	1.000000	85.7
rps12-clpP_p3	rps12-clpP	3	TGCACCAACGGAAACCATAA	TAGAAAGCAACGGAGCCATC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.041515	11748	11905	907-941	1.000000	85.7
rps12-clpP_p4	rps12-clpP	4	TTGCACCAACGGAAACCATA	TAGAAAGCAACGGAGCCATC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.041515	11748	11905	908-942	1.000000	85.7
rps12-clpP_p5	rps12-clpP	5	TGACCTTGGTAGTCCGTTCT	AAGCAACGGAGCCATCATAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046329	11748	11905	1046-1082	1.000000	85.6
petB_p1	petB	1	TTGGTAGTTCGATCGTGGAA	TCCTCCCCTTAGTGATAAATGA	20	22	45.0	40.9	56.6	55.7	15.9	0.0	5.722695	16748	18086	1433-1455	1.000000	44.7
petB_p2	petB	2	TTTGGTAGTTCGATCGTGGA	TCCTCCCCTTAGTGATAAATGA	20	22	45.0	40.9	56.6	55.7	15.9	0.0	5.722695	16748	18086	1434-1456	1.000000	44.7
petB_p3	petB	3	TGGTAGTTCGATCGTGGAAT	TCCTCCCCTTAGTGATAAATGA	20	22	45.0	40.9	56.4	55.7	15.9	0.0	5.952263	16748	18086	1432-1454	1.000000	44.7
petB_p4	petB	4	TTGGTAGTTCGATCGTGGAA	TTCCTCCCCTTAGTGATAAATGA	20	23	45.0	39.1	56.6	56.4	15.9	0.0	5.981225	16748	18086	1434-1456	1.000000	44.7
petB_p5	petB	5	TTTGGTAGTTCGATCGTGGA	TTCCTCCCCTTAGTGATAAATGA	20	23	45.0	39.1	56.6	56.4	15.9	0.0	5.981225	16748	18086	1435-1457	1.000000	44.7
rpl16_p1	rpl16	1	GGATTGCGCCAAAAATTCGA	CAAGTACAAATTGCAGGGCG	20	20	45.0	50.0	58.0	58.0	4.2	0.0	0.029316	22960	24227	2004-2021	0.500000	65.3
rpl16_p2	rpl16	2	TGCGCCAAAAATTCGAGTTC	CAAGTACAAATTGCAGGGCG	20	20	45.0	50.0	58.0	58.0	4.2	0.0	0.063634	22960	24227	2000-2017	0.500000	65.0
rpl16_p3	rpl16	3	TTGGGTGCCTCAAAATTGGA	CAAGTACAAATTGCAGGGCG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.112847	22960	24227	2227-2242	0.500000	64.2
rpl16_p4	rpl16	4	GGATTGCGCCAAAAATTCGA	GAAATTGCACGTGTCGAGTG	20	20	45.0	50.0	58.0	58.1	4.2	18.4	0.073210	22960	24227	1987-1988	0.333333	53.2
rpl16_p5	rpl16	5	TGCGCCAAAAATTCGAGTTC	GAAATTGCACGTGTCGAGTG	20	20	45.0	50.0	58.0	58.1	4.2	18.4	0.107528	22960	24227	1983-1984	0.333333	52.9
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GAACCTACGACTTCGCCAAT	GTTGAACCCTGTGAATTGCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.056448	27892	28070	354-439	1.000000	91.5
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	CCTGTGAATTGCGCGAAAAT	20	20	50.0	45.0	58.0	58.0	7.6	12.1	0.027947	27892	28070	327-407	0.833333	80.0
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	ATTGAACCTACGACTTCGCC	CCTGTGAATTGCGCGAAAAT	20	20	50.0	45.0	58.0	58.0	0.0	12.1	0.024717	27892	28070	355-435	0.833333	79.8
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GAACCTACGACTTCGCCAAT	CCTGTGAATTGCGCGAAAAT	20	20	50.0	45.0	58.0	58.0	0.0	12.1	0.024717	27892	28070	352-432	0.833333	79.8
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	TTCTACCCTTTCCCGGAAGT	CCTGTGAATTGCGCGAAAAT	20	20	50.0	45.0	58.0	58.0	0.7	12.1	0.053183	27892	28070	715-795	0.833333	78.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGTGCGACAAACAAATAAACT	TCCTAAGAGCAGCGTGTCTA	21	20	33.3	50.0	54.9	57.9	0.0	0.0	4.213031	52887	53324	479-515	1.000000	55.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGTGCGACAAACAAATAAACT	AGCAGCGTGTCTACCAATTT	21	20	33.3	45.0	54.9	57.8	0.0	0.0	4.273758	52887	53324	472-508	1.000000	54.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGTGCGACAAACAAATAAACT	CCTAAGAGCAGCGTGTCTAC	21	20	33.3	55.0	54.9	57.8	0.0	0.0	4.285049	52887	53324	478-514	1.000000	54.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGTGCGACAAACAAATAAACT	CTAAGAGCAGCGTGTCTACC	21	20	33.3	55.0	54.9	57.8	0.0	0.0	4.285049	52887	53324	477-513	1.000000	54.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGTGCGACAAACAAATAAACT	GAGCAGCGTGTCTACCAATT	21	20	33.3	50.0	54.9	58.3	0.0	0.0	4.361900	52887	53324	473-509	1.000000	54.2
ndhA_p1	ndhA	1	ACTGAGTTAGCATAGGGAACA	GCCGGGTTTTATCAACTTGC	21	20	42.9	50.0	56.1	58.0	0.0	2.2	2.932744	58733	60906	2286-2392	1.000000	62.6
ndhA_p2	ndhA	2	GAACTGAGTTAGCATAGGGAACA	GCCGGGTTTTATCAACTTGC	23	20	43.5	50.0	57.9	58.0	0.0	2.2	3.095304	58733	60906	2288-2394	1.000000	61.3
ndhA_p3	ndhA	3	ACTGAGTTAGCATAGGGAACA	TGCAAATTCTTCCGCAGCTA	21	20	42.9	45.0	56.1	58.2	0.0	0.0	3.103615	58733	60906	2269-2375	1.000000	61.2
ndhA_p4	ndhA	4	ACTGAGTTAGCATAGGGAACA	CGCCGGGTTTTATCAACTTG	21	20	42.9	50.0	56.1	57.7	0.0	2.2	3.179988	58733	60906	2287-2393	1.000000	60.6
ndhA_p5	ndhA	5	AACTGAGTTAGCATAGGGAACA	GCCGGGTTTTATCAACTTGC	22	20	40.9	50.0	56.8	58.0	0.0	2.2	3.187865	58733	60906	2287-2393	1.000000	60.5
ycf1_p1	ycf1	1	GAAACTCCTCCCAGACCTCT	TCATCCTCAACGCCCAAAAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.066127	66115	66234	745-745	0.333333	53.0
ycf1_p2	ycf1	2	GAAACTCCTCCCAGACCTCT	TTCATCCTCAACGCCCAAAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.066127	66115	66234	746-746	0.333333	53.0
ycf1_p3	ycf1	3	GAAACTCCTCCCAGACCTCT	TTTCATCCTCAACGCCCAAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.066127	66115	66234	747-747	0.333333	53.0
ycf1_p4	ycf1	4	GGGAATGCGCATTTGTTTGA	TCATCCTCAACGCCCAAAAA	20	20	45.0	45.0	57.9	58.0	34.2	0.0	0.089119	66115	66234	535-535	0.333333	52.9
ycf1_p5	ycf1	5	GGGAATGCGCATTTGTTTGA	TTCATCCTCAACGCCCAAAA	20	20	45.0	45.0	57.9	58.0	34.2	0.0	0.089119	66115	66234	536-536	0.333333	52.9
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	GTTGAACCCTGTGAATTGCG	GAACCTACGACTTCGCCAAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.056448	88970	89148	354-439	1.000000	91.5
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	CCTGTGAATTGCGCGAAAAT	GTTGGGCGCTTTAACCATTC	20	20	45.0	50.0	58.0	58.0	12.1	7.6	0.027947	88970	89148	327-407	0.833333	80.0
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	CCTGTGAATTGCGCGAAAAT	ATTGAACCTACGACTTCGCC	20	20	45.0	50.0	58.0	58.0	12.1	0.0	0.024717	88970	89148	355-435	0.833333	79.8
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	CCTGTGAATTGCGCGAAAAT	GAACCTACGACTTCGCCAAT	20	20	45.0	50.0	58.0	58.0	12.1	0.0	0.024717	88970	89148	352-432	0.833333	79.8
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	CCTGTGAATTGCGCGAAAAT	TTCTACCCTTTCCCGGAAGT	20	20	45.0	50.0	58.0	58.0	12.1	0.7	0.053183	88970	89148	715-795	0.833333	78.6
atpF-atpH_p1	atpF-atpH	1	TGGCCCAAAGAAACGAAAGA	CCTGAGGCAGAGGGAAAAAT	20	20	45.0	50.0	57.9	57.5	0.0	0.0	0.581597	105522	105875	511-515	0.500000	59.5
atpF-atpH_p2	atpF-atpH	2	GTGGCCCAAAGAAACGAAAG	CCTGAGGCAGAGGGAAAAAT	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.655748	105522	105875	512-516	0.500000	58.9
atpF-atpH_p3	atpF-atpH	3	AGTGGCCCAAAGAAACGAAA	CCTGAGGCAGAGGGAAAAAT	20	20	45.0	50.0	58.2	57.5	0.0	0.0	0.751252	105522	105875	513-517	0.500000	58.1
atpF-atpH_p4	atpF-atpH	4	TGGCCCAAAGAAACGAAAGA	GCAGAGGGAAAAATACGAGGT	20	21	45.0	47.6	57.9	58.0	0.0	0.0	1.072818	105522	105875	505-509	0.500000	55.6
atpF-atpH_p5	atpF-atpH	5	TTAAATCCGAAACTCCCGGC	CCTGAGGCAGAGGGAAAAAT	20	20	50.0	50.0	58.3	57.5	0.0	0.0	0.780019	105522	105875	541-542	0.333333	51.1