primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	CGCTTTCTACTTCAGGAACA	21	20	47.6	45.0	57.3	55.4	0.0	0.0	4.266441	8643	9004	411-425	1.000000	55.6
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GTTCAAATCCTATTGGACGCG	CGCTTTCTACTTCAGGAACA	21	20	47.6	45.0	57.7	55.4	3.5	0.0	3.892888	8643	9004	410-424	0.666667	42.8
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GTTCAAATCCTATTGGACGCG	CGCTTTCTACTTCAGGAACAAG	21	22	47.6	45.5	57.7	57.3	3.5	0.0	3.973044	8643	9004	410-424	0.666667	42.1
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GTTCAAATCCTATTGGACGCG	CGCTTTCTACTTCAGGAACAA	21	21	47.6	42.9	57.7	56.2	3.5	0.0	4.107766	8643	9004	410-424	0.666667	41.1
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TTCAAATCCTATTGGACGCG	CGCTTTCTACTTCAGGAACA	20	20	45.0	45.0	56.2	55.4	3.5	0.0	4.345957	8643	9004	409-423	0.666667	39.2
petN-psbM_p1	petN-psbM	1	AAGTCTTTCTTGGGCTGCTT	TGGAAGTAAACATTCTCGCA	20	20	45.0	40.0	57.6	55.0	0.0	0.0	3.363870	27824	28511	860-871	1.000000	58.3
petN-psbM_p2	petN-psbM	2	AGTCTTTCTTGGGCTGCTTT	TGGAAGTAAACATTCTCGCA	20	20	45.0	40.0	57.6	55.0	0.0	0.0	3.363870	27824	28511	859-870	1.000000	58.3
petN-psbM_p3	petN-psbM	3	AAGTCTTTCTTGGGCTGCTT	TGTTCTAGTTCCAACTGCTTTCT	20	23	45.0	39.1	57.6	58.0	0.0	2.0	3.407775	27824	28511	820-831	1.000000	58.2
petN-psbM_p4	petN-psbM	4	AGTCTTTCTTGGGCTGCTTT	TGTTCTAGTTCCAACTGCTTTCT	20	23	45.0	39.1	57.6	58.0	0.0	2.0	3.407775	27824	28511	819-830	1.000000	58.2
petN-psbM_p5	petN-psbM	5	TCTTGGGCTGCTTTAATGGT	TGGAAGTAAACATTCTCGCA	20	20	45.0	40.0	57.4	55.0	0.0	0.0	3.595287	27824	28511	853-864	1.000000	56.4
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	GAGGCTGCATTAATCGGGAA	CTTGATTCATCCCCGCATGA	20	20	50.0	50.0	58.0	58.0	0.5	0.0	0.061200	44379	44955	1435-1460	1.000000	84.7
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	GAGGCTGCATTAATCGGGAA	TCTTGATTCATCCCCGCATG	20	20	50.0	50.0	58.0	58.0	0.5	0.0	0.061200	44379	44955	1436-1461	1.000000	84.7
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	GAGGCTGCATTAATCGGGAA	TGATTCATCCCCGCATGAAG	20	20	50.0	50.0	58.0	58.0	0.5	0.0	0.061200	44379	44955	1433-1458	1.000000	84.7
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTGTTTCCAACGCCTCTTCA	TGATTCATCCCCGCATGAAG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.074080	44379	44955	1742-1769	1.000000	84.1
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTGTTTCCAACGCCTCTTCA	CTTGATTCATCCCCGCATGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.074080	44379	44955	1744-1771	1.000000	84.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	58.2	58.2	0.0	10.2	0.406999	46550	47335	832-878	1.000000	84.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	58.2	57.6	0.0	0.0	0.569336	46550	47335	826-872	1.000000	83.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAAAGCCAGCTATCGGAGTC	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.7	58.2	0.0	10.2	0.565978	46550	47335	847-893	1.000000	83.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAAAAGCCAGCTATCGGAGT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.7	58.2	0.0	10.2	0.565978	46550	47335	848-894	1.000000	83.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAAAGCCAGCTATCGGAGTC	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.7	57.6	0.0	0.0	0.728315	46550	47335	841-887	1.000000	81.8
trnF-GAA-trnV-UAC_p1	trnF-GAA-trnV-UAC	1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.249179	48231	48618	432-455	1.000000	87.4
trnF-GAA-trnV-UAC_p2	trnF-GAA-trnV-UAC	2	AGTTCAAATCTGGTTCCCGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.397656	48231	48618	420-443	1.000000	86.4
trnF-GAA-trnV-UAC_p3	trnF-GAA-trnV-UAC	3	AATGGTCGGGATAGCTCAGT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.402569	48231	48618	475-498	1.000000	85.7
trnF-GAA-trnV-UAC_p4	trnF-GAA-trnV-UAC	4	ATGGTCGGGATAGCTCAGTT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.402569	48231	48618	474-497	1.000000	85.7
trnF-GAA-trnV-UAC_p5	trnF-GAA-trnV-UAC	5	CCTCGTGTCACCAGTTCAAA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.1	57.5	0.0	0.0	0.592951	48231	48618	431-454	1.000000	84.7
atpB-rbcL_p1	atpB-rbcL	1	GGGCGATATGTCCCATGTTT	CGGTCTCTCCAACGCATAAA	20	20	50.0	50.0	58.0	58.0	4.4	0.0	0.023326	51730	52534	1523-1528	1.000000	86.3
atpB-rbcL_p2	atpB-rbcL	2	GGGCGATATGTCCCATGTTT	TCTCTCCAACGCATAAACGG	20	20	50.0	50.0	58.0	58.0	4.4	0.0	0.023326	51730	52534	1520-1525	1.000000	86.3
atpB-rbcL_p3	atpB-rbcL	3	TTGTCAACGGGTTCTCCAAG	TCTCTCCAACGCATAAACGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.042789	51730	52534	1806-1811	1.000000	85.5
atpB-rbcL_p4	atpB-rbcL	4	TTGTCAACGGGTTCTCCAAG	CGGTCTCTCCAACGCATAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.042789	51730	52534	1809-1814	1.000000	85.4
atpB-rbcL_p5	atpB-rbcL	5	AAAGCGGGTGCAGATCTATG	TCTCTCCAACGCATAAACGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045541	51730	52534	1863-1868	1.000000	85.4
rbcL-accD_p1	rbcL-accD	1	TCATGGTATGCACTTCCGTG	TAGGATTTCCAGCACCAGGA	20	20	50.0	50.0	58.0	58.0	15.5	0.0	0.057350	53963	54564	1144-1144	0.333333	52.9
rbcL-accD_p2	rbcL-accD	2	AATGAGGGACGTGATCTTGC	TAGGATTTCCAGCACCAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.064424	53963	54564	768-768	0.333333	52.8
rbcL-accD_p3	rbcL-accD	3	GGCTGCATGTGCTAGAGAAT	TAGGATTTCCAGCACCAGGA	20	20	50.0	50.0	58.0	58.0	6.5	0.0	0.070033	53963	54564	1303-1303	0.333333	52.8
rbcL-accD_p4	rbcL-accD	4	TCTTCACATTCACCGTGCAA	TAGGATTTCCAGCACCAGGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.074169	53963	54564	1192-1192	0.333333	52.7
rbcL-accD_p5	rbcL-accD	5	TTCTTCACATTCACCGTGCA	TAGGATTTCCAGCACCAGGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.074169	53963	54564	1193-1193	0.333333	52.7
accD_p1	accD	1	AATGAGGGACGTGATCTTGC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.056842	54565	55926	2565-2580	1.000000	91.2
accD_p2	accD	2	AATGAGGGACGTGATCTTGC	TTGCCGGAAATACTAAGCCC	20	20	50.0	50.0	58.0	58.0	0.0	5.0	0.076840	54565	55926	2556-2571	1.000000	91.1
accD_p3	accD	3	GTGATCTTGCTCGTGAAGGT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	26.9	0.113058	54565	55926	2555-2570	1.000000	90.8
accD_p4	accD	4	CGGTGCTGAAGATGATGGAA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.055423	54565	55926	1877-1877	0.333333	52.9
accD_p5	accD	5	CGGTGCTGAAGATGATGGAA	TTGCCGGAAATACTAAGCCC	20	20	50.0	50.0	58.0	58.0	0.0	5.0	0.075422	54565	55926	1868-1868	0.333333	52.7
psbE-petL_p1	psbE-petL	1	AGCAAAAGAACGTTCTCCTGT	AGCTGCCAGTAGAAAACCAA	21	20	42.9	45.0	58.1	57.3	29.8	0.0	1.737720	60911	62201	1321-1366	1.000000	76.6
psbE-petL_p2	psbE-petL	2	AGCAAAAGAACGTTCTCCTGT	AAGCTGCCAGTAGAAAACCA	21	20	42.9	45.0	58.1	57.3	29.8	0.0	1.737720	60911	62201	1322-1367	1.000000	76.5
psbE-petL_p3	psbE-petL	3	ACGTTCTCCTGTACTTCCAGA	AAGCTGCCAGTAGAAAACCA	21	20	47.6	45.0	58.1	57.3	0.0	0.0	1.784215	60911	62201	1313-1358	1.000000	76.2
psbE-petL_p4	psbE-petL	4	ACGTTCTCCTGTACTTCCAGA	AGCTGCCAGTAGAAAACCAA	21	20	47.6	45.0	58.1	57.3	0.0	0.0	1.784215	60911	62201	1312-1357	1.000000	76.2
psbE-petL_p5	psbE-petL	5	TCAGCAAAAGAACGTTCTCCT	AGCTGCCAGTAGAAAACCAA	21	20	42.9	45.0	57.8	57.3	29.8	0.0	1.850952	60911	62201	1323-1368	1.000000	75.6
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	CGGTGCCACAGAGAGATATG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.245712	63091	63503	504-504	1.000000	83.6
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	CGGTGCCACAGAGAGATATG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.247056	63091	63503	503-503	1.000000	83.6
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACCCGTGACATTTTGTACCC	TAATACCGGTGCCACAGAGA	20	20	50.0	50.0	58.1	58.1	0.0	19.9	0.244459	63091	63503	510-510	1.000000	83.5
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CCCGTGACATTTTGTACCCA	TAATACCGGTGCCACAGAGA	20	20	50.0	50.0	58.1	58.1	0.0	19.9	0.245803	63091	63503	509-509	1.000000	83.5
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	ACAGGATTTGAACCCGTGAC	TAATACCGGTGCCACAGAGA	20	20	50.0	50.0	58.1	58.1	0.0	19.9	0.252495	63091	63503	521-521	1.000000	83.4
rpl33-rps18_p1	rpl33-rps18	1	AAATCGACACAATACGCCGA	AAACTCCGGGAAGGTAGAGT	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.323290	64322	64954	1285-1305	1.000000	89.3
rpl33-rps18_p2	rpl33-rps18	2	AATCGACACAATACGCCGAA	AAACTCCGGGAAGGTAGAGT	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.323290	64322	64954	1284-1304	1.000000	89.3
rpl33-rps18_p3	rpl33-rps18	3	AAATCGACACAATACGCCGA	AGAGTAAACTCCGGGAAGGT	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.323290	64322	64954	1290-1310	1.000000	89.2
rpl33-rps18_p4	rpl33-rps18	4	AATCGACACAATACGCCGAA	AGAGTAAACTCCGGGAAGGT	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.323290	64322	64954	1289-1309	1.000000	89.2
rpl33-rps18_p5	rpl33-rps18	5	TTTTCCCAGATGCGTTGACA	AAACTCCGGGAAGGTAGAGT	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.348074	64322	64954	1910-1938	1.000000	86.4
rps18_p1	rps18	1	AAATCGACACAATACGCCGA	AAACTCCGGGAAGGTAGAGT	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.323290	64955	65260	1285-1305	1.000000	89.3
rps18_p2	rps18	2	AATCGACACAATACGCCGAA	AAACTCCGGGAAGGTAGAGT	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.323290	64955	65260	1284-1304	1.000000	89.3
rps18_p3	rps18	3	AAATCGACACAATACGCCGA	AGAGTAAACTCCGGGAAGGT	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.323290	64955	65260	1290-1310	1.000000	89.2
rps18_p4	rps18	4	AATCGACACAATACGCCGAA	AGAGTAAACTCCGGGAAGGT	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.323290	64955	65260	1289-1309	1.000000	89.2
rps18_p5	rps18	5	AATCGACACAATACGCCGAA	GTAGTTTGTGGATCACCCGT	20	20	45.0	50.0	58.0	57.5	0.0	0.0	0.468518	64955	65260	1535-1555	1.000000	86.8
rps18-rpl20_p1	rps18-rpl20	1	CGATCTTTTCGTAGGCGGTT	AATGAAGAAAAGGGCTCCGG	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.523588	65261	65604	1306-1334	0.666667	72.1
rps18-rpl20_p2	rps18-rpl20	2	CGATCTTTTCGTAGGCGGTT	AAAGGGCTCCGGTGTATAGA	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.533024	65261	65604	1298-1326	0.666667	72.0
rps18-rpl20_p3	rps18-rpl20	3	CGATCTTTTCGTAGGCGGTT	GTAGTTTGTGGATCACCCGT	20	20	50.0	50.0	58.4	57.5	0.0	0.0	0.830578	65261	65604	772-796	0.666667	71.6
rps18-rpl20_p4	rps18-rpl20	4	CGATCTTTTCGTAGGCGGTT	AAGGGCTCCGGTGTATAGAG	20	20	50.0	55.0	58.4	58.3	0.0	0.0	0.657139	65261	65604	1297-1325	0.666667	71.1
rps18-rpl20_p5	rps18-rpl20	5	CGATCTTTTCGTAGGCGGTT	GGGCTCCGGTGTATAGAGAA	20	20	50.0	55.0	58.4	58.3	0.0	0.0	0.662057	65261	65604	1295-1323	0.666667	71.0
clpP_p1	clpP	1	TGACCCTGTTAGTCCGTTCT	AGCAACAAGAGCTGTATGCA	20	20	50.0	45.0	58.0	57.8	0.0	15.5	0.194964	67036	68472	2576-2587	1.000000	83.2
clpP_p2	clpP	2	TGACCCTGTTAGTCCGTTCT	ACCAAGGCAAACCCATGAAA	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.205437	67036	68472	2499-2510	1.000000	83.2
clpP_p3	clpP	3	TGACCCTGTTAGTCCGTTCT	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.242663	67036	68472	2503-2514	1.000000	82.8
clpP_p4	clpP	4	TGACCCTGTTAGTCCGTTCT	CAAACGACCGGGATCATTCA	20	20	50.0	50.0	58.0	58.3	0.0	6.3	0.272430	67036	68472	2540-2551	1.000000	82.6
clpP_p5	clpP	5	TGACCCTGTTAGTCCGTTCT	CATACCCTGCCTCCACATTG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.321427	67036	68472	2663-2674	1.000000	82.1
rpoA_p1	rpoA	1	CATTTCGTCGTCCAGTAGCA	GCAGGAAATGCGATTCGAAC	20	20	50.0	50.0	58.0	58.2	0.0	10.9	0.194069	74513	75523	1517-1533	1.000000	86.8
rpoA_p2	rpoA	2	TAATGGTTTCGGTACCTGCG	GCAGGAAATGCGATTCGAAC	20	20	50.0	50.0	58.0	58.2	10.9	10.9	0.197805	74513	75523	1586-1602	1.000000	86.7
rpoA_p3	rpoA	3	TTATGGAGAACCTGCATGGC	GCAGGAAATGCGATTCGAAC	20	20	50.0	50.0	57.9	58.2	10.6	10.9	0.242005	74513	75523	1807-1823	1.000000	85.9
rpoA_p4	rpoA	4	CATTTCGTCGTCCAGTAGCA	GGTGATTTCTTGGTCCTCCG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.270134	74513	75523	1596-1612	1.000000	85.9
rpoA_p5	rpoA	5	CTCGAGCCGGATGATGAAAA	GCAGGAAATGCGATTCGAAC	20	20	50.0	50.0	58.1	58.2	5.0	10.9	0.244893	74513	75523	1952-1968	1.000000	85.4