primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbC-atpF_p1	psbC-atpF	1	GAGCTACGTGTGCACCTAAA	CAAGAAGCAAGCCAAAGAGC	20	20	50.0	50.0	57.9	57.9	15.7	0.0	0.136287	1387	2019	1117-1320	0.800000	81.1
psbC-atpF_p2	psbC-atpF	2	GAGCTACGTGTGCACCTAAA	AGGTGTATTCCGAGCAACAC	20	20	50.0	50.0	57.9	57.9	15.7	0.0	0.169309	1387	2019	1016-1219	0.800000	81.0
psbC-atpF_p3	psbC-atpF	3	ACAATCCAACCATCACCACC	CAAGAAGCAAGCCAAAGAGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.138384	1387	2019	1625-1828	0.800000	80.4
psbC-atpF_p4	psbC-atpF	4	CAATCCAACCATCACCACCA	CAAGAAGCAAGCCAAAGAGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.139758	1387	2019	1624-1827	0.800000	80.4
psbC-atpF_p5	psbC-atpF	5	GGTAAACACCCCCAAAACCT	CAAGAAGCAAGCCAAAGAGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.152838	1387	2019	1345-1548	0.600000	72.6
rpoC2-rps3_p1	rpoC2-rps3	1	CGTTGCATACGTTCTACTCCT	ATTGCACGTTCAGAATGGGT	21	20	47.6	45.0	57.9	58.1	11.6	6.5	1.236918	22769	23401	1466-1478	0.400000	49.6
rpoC2-rps3_p2	rpoC2-rps3	2	CGTTGCATACGTTCTACTCCT	GCAGGTCGTGTTCCTTTACA	21	20	47.6	50.0	57.9	58.1	11.6	0.0	1.272920	22769	23401	1442-1454	0.400000	49.4
rpoC2-rps3_p3	rpoC2-rps3	3	ACTTTTGCCCTTCTTGACTCA	ATTGCACGTTCAGAATGGGT	21	20	42.9	45.0	57.7	58.1	0.0	6.5	1.387435	22769	23401	1004-1016	0.400000	49.1
rpoC2-rps3_p4	rpoC2-rps3	4	CGTTGCATACGTTCTACTCCT	GTGCAGGTCGTGTTCCTTTA	21	20	47.6	50.0	57.9	58.1	11.6	0.0	1.272920	22769	23401	1444-1444	0.200000	37.8
rpoC2-rps3_p5	rpoC2-rps3	5	CGTTGCATACGTTCTACTCCT	TGCAGGTCGTGTTCCTTTAC	21	20	47.6	50.0	57.9	58.1	11.6	0.0	1.272920	22769	23401	1443-1443	0.200000	37.8
rpoBb-rpoBa_p1	rpoBb-rpoBa	1	ACCATTGGACGCTGAACAAT	GGAGGTGTGTCACGTGATAC	20	20	45.0	55.0	58.1	58.0	10.4	31.4	0.098131	30482	31129	1553-1570	0.400000	58.5
rpoBb-rpoBa_p2	rpoBb-rpoBa	2	ACCATTGGACGCTGAACAAT	TTGCGTTAATGGGGGTTTCA	20	20	45.0	45.0	58.1	58.0	10.4	0.0	0.093502	30482	31129	1204-1204	0.200000	47.3
rpoBb-rpoBa_p3	rpoBb-rpoBa	3	ACCATTGGACGCTGAACAAT	AAACTTGCGTTAATGGGGGT	20	20	45.0	45.0	58.1	58.0	10.4	0.0	0.094237	30482	31129	1208-1208	0.200000	47.2
rpoBb-rpoBa_p4	rpoBb-rpoBa	4	ACCATTGGACGCTGAACAAT	AACTTGCGTTAATGGGGGTT	20	20	45.0	45.0	58.1	58.0	10.4	0.0	0.094237	30482	31129	1207-1207	0.200000	47.2
rpoBb-rpoBa_p5	rpoBb-rpoBa	5	ACCATTGGACGCTGAACAAT	ACTTGCGTTAATGGGGGTTT	20	20	45.0	45.0	58.1	58.0	10.4	0.0	0.094237	30482	31129	1206-1206	0.200000	47.2
psbL-V9305_pgt002_p1	psbL-V9305_pgt002	1	TTCACGCATTAGCAGTTCCT	GGACTTGAACCCTCACGATC	20	20	45.0	55.0	57.5	58.0	0.0	0.0	0.484236	35615	36360	1429-1831	1.000000	88.1
psbL-V9305_pgt002_p2	psbL-V9305_pgt002	2	TTCACGCATTAGCAGTTCCT	AAGTTGCTTGATCACCACGA	20	20	45.0	45.0	57.5	57.7	0.0	0.0	0.727241	35615	36360	2073-2422	1.000000	86.2
psbL-V9305_pgt002_p3	psbL-V9305_pgt002	3	TTCACGCATTAGCAGTTCCT	CAAGTTGCTTGATCACCACG	20	20	45.0	50.0	57.5	57.7	0.0	0.0	0.793501	35615	36360	2074-2423	1.000000	85.7
psbL-V9305_pgt002_p4	psbL-V9305_pgt002	4	TTCACGCATTAGCAGTTCCT	ACCAAGTTGCTTGATCACCA	20	20	45.0	45.0	57.5	57.6	0.0	10.1	0.836002	35615	36360	2076-2425	1.000000	85.3
psbL-V9305_pgt002_p5	psbL-V9305_pgt002	5	AGCCAACAGTCAAGTCAACT	GGACTTGAACCCTCACGATC	20	20	45.0	55.0	57.3	58.0	0.0	0.0	0.729179	35615	36360	1189-1240	0.400000	53.3
psbJ-psaA_p1	psbJ-psaA	1	ACCAAGAGCTGCTGTACCTA	ACCACACGAACGCTTAGTTT	20	20	50.0	45.0	58.1	58.1	7.0	0.0	0.117384	40023	40987	1756-1778	0.400000	58.1
psbJ-psaA_p2	psbJ-psaA	2	TACCAAGAGCTGCTGTACCT	ACCACACGAACGCTTAGTTT	20	20	50.0	45.0	58.1	58.1	7.0	0.0	0.117384	40023	40987	1757-1779	0.400000	58.1
psbJ-psaA_p3	psbJ-psaA	3	CCAAGAGCTGCTGTACCTAC	ACCACACGAACGCTTAGTTT	20	20	55.0	45.0	57.7	58.1	7.0	0.0	0.357546	40023	40987	1755-1777	0.400000	56.2
psbJ-psaA_p4	psbJ-psaA	4	ACCAAGAGCTGCTGTACCTA	GCGTATACGGTTCTGTGGAA	20	20	50.0	50.0	58.1	57.7	7.0	25.6	0.344116	40023	40987	1283-1283	0.200000	45.2
psbJ-psaA_p5	psbJ-psaA	5	TACCAAGAGCTGCTGTACCT	GCGTATACGGTTCTGTGGAA	20	20	50.0	50.0	58.1	57.7	7.0	25.6	0.344116	40023	40987	1284-1284	0.200000	45.2
rpl20-rbcL_p1	rpl20-rbcL	1	ATAATGAAGCTGCTCCACGG	CCTGGTTGTGGAGTCATACG	20	20	50.0	55.0	58.0	58.0	1.0	0.0	0.051103	62126	62802	882-1001	0.800000	83.6
rpl20-rbcL_p2	rpl20-rbcL	2	ATAATGAAGCTGCTCCACGG	CTGGTTGTGGAGTCATACGG	20	20	50.0	55.0	58.0	58.0	1.0	0.0	0.051103	62126	62802	881-1000	0.800000	83.6
rpl20-rbcL_p3	rpl20-rbcL	3	ATAATGAAGCTGCTCCACGG	GCACGAAGAGCTTTGAAACC	20	20	50.0	50.0	58.0	58.0	1.0	4.5	0.078899	62126	62802	1137-1256	0.800000	83.4
rpl20-rbcL_p4	rpl20-rbcL	4	ATAATGAAGCTGCTCCACGG	TCTCTCCAACGCATGAATGG	20	20	50.0	50.0	58.0	58.0	1.0	0.0	0.069378	62126	62802	1389-1472	0.600000	73.9
rpl20-rbcL_p5	rpl20-rbcL	5	ATAATGAAGCTGCTCCACGG	GGTCTCTCCAACGCATGAAT	20	20	50.0	50.0	58.0	58.0	1.0	0.0	0.070797	62126	62802	1391-1474	0.600000	73.9
psbA_p1	psbA	1	AAATCTAAGCTTGAGTTTTTCA	TCTCTAAACAGTACACCGAA	22	20	27.3	40.0	52.2	53.4	9.9	0.0	12.387638	66620	69350	2917-2917	0.200000	8.0
psbA_p2	psbA	2	AAATCTAAGCTTGAGTTTTTCA	TTCTCTAAACAGTACACCGA	22	20	27.3	40.0	52.2	53.4	9.9	0.0	12.387638	66620	69350	2918-2918	0.200000	8.0
psbA_p3	psbA	3	TGAAAAACTCAAGCTTAAATCT	TCTCTAAACAGTACACCGAA	22	20	27.3	40.0	52.2	53.4	0.0	0.0	12.387638	66620	69350	2933-2933	0.200000	8.0
psbA_p4	psbA	4	TGAAAAACTCAAGCTTAAATCT	TTCTCTAAACAGTACACCGA	22	20	27.3	40.0	52.2	53.4	0.0	0.0	12.387638	66620	69350	2934-2934	0.200000	8.0
psbA_p5	psbA	5	AAATCTAAGCTTGAGTTTTTCA	TTCTCTAAACAGTACACCGAA	22	21	27.3	38.1	52.2	54.3	9.9	0.0	12.492754	66620	69350	2918-2918	0.200000	8.0
psbA-V9305_pgt009_p1	psbA-V9305_pgt009	1	CCAAAGGCTAGATGCTTCGT	GGAGTCACAATCCATTGCCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.164627	69351	71010	1742-2624	0.600000	74.7
psbA-V9305_pgt009_p2	psbA-V9305_pgt009	2	CCAAAGGCTAGATGCTTCGT	TGGAGTCACAATCCATTGCC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.191726	69351	71010	1743-2625	0.600000	74.5
psbA-V9305_pgt009_p3	psbA-V9305_pgt009	3	CCAAAGGCTAGATGCTTCGT	TTGCCTTACCACTTGGCTAC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.224040	69351	71010	1728-2610	0.600000	74.2
psbA-V9305_pgt009_p4	psbA-V9305_pgt009	4	CCAAAGGCTAGATGCTTCGT	TCCATTGCCTTACCACTTGG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.244214	69351	71010	1732-2614	0.600000	74.0
psbA-V9305_pgt009_p5	psbA-V9305_pgt009	5	CCAAAGGCTAGATGCTTCGT	CATTGCCTTACCACTTGGCT	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.480635	69351	71010	1730-2612	0.600000	72.2
V9305_pgt009-V9305_pgr006_p1	V9305_pgt009-V9305_pgr006	1	ACATATTTTATGGCGGGCGT	TCTCGTACGACTTGCATGTG	20	20	45.0	50.0	58.0	58.0	0.7	15.3	0.047152	71083	72522	1562-1605	0.600000	75.6
V9305_pgt009-V9305_pgr006_p2	V9305_pgt009-V9305_pgr006	2	ACATATTTTATGGCGGGCGT	CCCGCCAAAAGGTAAGTTCT	20	20	45.0	50.0	58.0	58.1	0.7	0.0	0.118703	71083	72522	1636-1703	0.600000	75.1
V9305_pgt009-V9305_pgr006_p3	V9305_pgt009-V9305_pgr006	3	ACATATTTTATGGCGGGCGT	GAACCACGATCCTTCATCCC	20	20	45.0	55.0	58.0	58.0	0.7	0.0	0.069413	71083	72522	1973-1973	0.200000	47.4
V9305_pgt009-V9305_pgr006_p4	V9305_pgt009-V9305_pgr006	4	TGTTCTGATTCTCTTGGCCG	TCTCGTACGACTTGCATGTG	20	20	50.0	50.0	57.9	58.0	0.0	15.3	0.121953	71083	72522	1974-1974	0.200000	47.0
V9305_pgt009-V9305_pgr006_p5	V9305_pgt009-V9305_pgr006	5	TGTTCTGATTCTCTTGGCCG	GAACCACGATCCTTCATCCC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144214	71083	72522	2342-2342	0.200000	46.8
V9305_pgt014-V9305_pgt015_p1	V9305_pgt014-V9305_pgt015	1	TCCTCTGCTCTACCACTGAG	GAGGTGTCCTAGGCCTCTAG	20	20	55.0	60.0	57.9	58.0	0.0	21.0	0.146592	91959	92502	605-793	1.000000	90.8
V9305_pgt014-V9305_pgt015_p2	V9305_pgt014-V9305_pgt015	2	TCCTCTGCTCTACCACTGAG	TGAAAGGGAGGTGTCCTAGG	20	20	55.0	55.0	57.9	58.0	0.0	0.8	0.183677	91959	92502	612-800	1.000000	90.5
V9305_pgt014-V9305_pgt015_p3	V9305_pgt014-V9305_pgt015	3	TCCTCTGCTCTACCACTGAG	TTCGAATCCCCGTTTTCTCC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.239836	91959	92502	633-821	1.000000	90.1
V9305_pgt014-V9305_pgt015_p4	V9305_pgt014-V9305_pgt015	4	TTTGCCAGGAACCAGGATTG	GAGGTGTCCTAGGCCTCTAG	20	20	50.0	60.0	58.4	58.0	6.5	21.0	0.378889	91959	92502	649-837	1.000000	89.0
V9305_pgt014-V9305_pgt015_p5	V9305_pgt014-V9305_pgt015	5	TTTTGCCAGGAACCAGGATT	GAGGTGTCCTAGGCCTCTAG	20	20	45.0	60.0	57.6	58.0	6.5	21.0	0.407337	91959	92502	650-838	1.000000	88.7
atpB-V9305_pgt019_p1	atpB-V9305_pgt019	1	TTTTCCTACACCAGCACCAC	GGTTCGAATCCTGTCATCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.069413	104967	105741	1298-1502	1.000000	91.4
atpB-V9305_pgt019_p2	atpB-V9305_pgt019	2	CAATTTTTCCACCACGACGG	CAGTAGGTGCTGGTGTTGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077569	104967	105741	1920-2377	0.800000	83.4
atpB-V9305_pgt019_p3	atpB-V9305_pgt019	3	CCAATTTTTCCACCACGACG	CAGTAGGTGCTGGTGTTGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077569	104967	105741	1921-2378	0.800000	83.4
atpB-V9305_pgt019_p4	atpB-V9305_pgt019	4	GCAGTTAAAGCAACACGCAT	CAGTAGGTGCTGGTGTTGTT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.083158	104967	105741	2173-2630	0.800000	83.3
atpB-V9305_pgt019_p5	atpB-V9305_pgt019	5	CAATTTTTCCACCACGACGG	GGTTCGAATCCTGTCATCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.089961	104967	105741	1269-1425	0.800000	83.3
psbM-psbZ_p1	psbM-psbZ	1	TGCTGTTGCTGTTAATCCAA	TCTTTTAGTTTTTGCAGTTGGT	20	22	40.0	31.8	55.9	55.0	0.0	0.0	7.196354	108707	109506	882-1325	1.000000	52.0
psbM-psbZ_p2	psbM-psbZ	2	TGCTGTTGCTGTTAATCCAAAG	TCTTTTAGTTTTTGCAGTTGGT	22	22	40.9	31.8	57.8	55.0	0.0	0.0	7.290808	108707	109506	882-1325	1.000000	52.0
psbM-psbZ_p3	psbM-psbZ	3	TGCTGTTGCTGTTAATCCAAA	TCTTTTAGTTTTTGCAGTTGGT	21	22	38.1	31.8	56.6	55.0	0.0	0.0	7.419035	108707	109506	882-1325	1.000000	52.0
psbM-psbZ_p4	psbM-psbZ	4	ATGCTGTTGCTGTTAATCCA	TCTTTTAGTTTTTGCAGTTGGT	20	22	40.0	31.8	55.6	55.0	0.0	0.0	7.430433	108707	109506	883-1326	1.000000	52.0
psbM-psbZ_p5	psbM-psbZ	5	TGCTGTTGCTGTTAATCCAA	TTCTTTTAGTTTTTGCAGTTGGT	20	23	40.0	30.4	55.9	55.7	0.0	0.0	7.443738	108707	109506	883-1326	1.000000	52.0
psaB-V9305_pgt022_p1	psaB-V9305_pgt022	1	CGAGTTGTTGGATCTTGTGC	CCTAGGACACCTCCCTTTCA	20	20	50.0	55.0	57.4	58.0	0.0	1.1	0.648859	114392	115680	1403-1719	1.000000	86.8
psaB-V9305_pgt022_p2	psaB-V9305_pgt022	2	TCTTGTGCTAAACCTTGGCT	CCTAGGACACCTCCCTTTCA	20	20	45.0	55.0	57.3	58.0	0.0	1.1	0.704326	114392	115680	1391-1707	1.000000	86.4
psaB-V9305_pgt022_p3	psaB-V9305_pgt022	3	CGAGTTGTTGGATCTTGTGC	GGAGAAAACGGGGATTCGAA	20	20	50.0	50.0	57.4	57.9	0.0	0.0	0.705018	114392	115680	1382-1698	1.000000	86.4
psaB-V9305_pgt022_p4	psaB-V9305_pgt022	4	CGAGTTGTTGGATCTTGTGC	TCCCTTTCACGGAGAAAACG	20	20	50.0	50.0	57.4	58.1	0.0	6.7	0.727783	114392	115680	1392-1708	1.000000	86.2
psaB-V9305_pgt022_p5	psaB-V9305_pgt022	5	TCTTGTGCTAAACCTTGGCT	GGAGAAAACGGGGATTCGAA	20	20	45.0	50.0	57.3	57.9	0.0	0.0	0.760484	114392	115680	1370-1686	1.000000	85.9
V9305_pgt028-V9305_pgt029_p1	V9305_pgt028-V9305_pgt029	1	ATGCAGAACACAAGCACTCC	ATTTTGTGGGGACAAGCCAA	20	20	50.0	45.0	58.8	58.2	0.0	0.0	0.957990	141822	141941	468-468	0.200000	40.3
V9305_pgt028-V9305_pgt029_p2	V9305_pgt028-V9305_pgt029	2	ATGCAGAACACAAGCACTCC	GTGGGGACAAGCCAAAAGAT	20	20	50.0	50.0	58.8	58.4	0.0	0.0	1.125335	141822	141941	463-463	0.200000	39.0
V9305_pgt028-V9305_pgt029_p3	V9305_pgt028-V9305_pgt029	3	ATGCAGAACACAAGCACTCC	TGGGGACAAGCCAAAAGATT	20	20	50.0	45.0	58.8	57.6	0.0	0.0	1.154967	141822	141941	462-462	0.200000	38.8
V9305_pgt028-V9305_pgt029_p4	V9305_pgt028-V9305_pgt029	4	ATGCAGAACACAAGCACTCC	TTTGTGGGGACAAGCCAAAA	20	20	50.0	45.0	58.8	58.4	0.0	0.0	1.184816	141822	141941	466-466	0.200000	38.5
V9305_pgt028-V9305_pgt029_p5	V9305_pgt028-V9305_pgt029	5	ATGCAGAACACAAGCACTCC	TTTTGTGGGGACAAGCCAAA	20	20	50.0	45.0	58.8	58.4	0.0	0.0	1.184816	141822	141941	467-467	0.200000	38.5