primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score matK_p1 matK 1 TCAGAATGGAAAACGTGAAAGT CAACACCCTGTTCTGACCAT 22 20 36.4 50.0 56.5 57.7 0.0 0.0 3.787067 1680 3215 1699-1699 1.000000 49.9 matK_p2 matK 2 TCAGAATGGAAAACGTGAAAGT AATGCAACACCCTGTTCTGA 22 20 36.4 45.0 56.5 57.6 0.0 0.0 3.873641 1680 3215 1703-1703 1.000000 49.2 matK_p3 matK 3 TCAGAATGGAAAACGTGAAAGT ATGCAACACCCTGTTCTGAC 22 20 36.4 50.0 56.5 58.4 0.0 0.0 3.901127 1680 3215 1702-1702 1.000000 49.0 matK_p4 matK 4 ACATTTCAGAATGGAAAACGTGA CAACACCCTGTTCTGACCAT 23 20 34.8 50.0 57.2 57.7 0.0 0.0 4.042883 1680 3215 1704-1704 1.000000 47.9 matK_p5 matK 5 AGGGAACATTTCAGAATGGA CAACACCCTGTTCTGACCAT 20 20 40.0 50.0 54.2 57.7 4.4 0.0 4.081798 1680 3215 1709-1709 1.000000 47.6 psbK-psbI_p1 psbK-psbI 1 CGTGGATGTTATGCCTGTCA TTTTTACTCCTCACGCCCAG 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.201676 7272 7670 592-592 1.000000 79.6 psbK-psbI_p2 psbK-psbI 2 GTTTGGCAAGCTGCTGTAAG TTTTTACTCCTCACGCCCAG 20 20 50.0 50.0 57.9 57.8 1.4 0.0 0.235006 7272 7670 543-543 1.000000 79.4 psbK-psbI_p3 psbK-psbI 3 TGGCAAGCTGCTGTAAGTTT TTTTTACTCCTCACGCCCAG 20 20 45.0 50.0 58.3 57.8 1.4 0.0 0.487716 7272 7670 540-540 1.000000 77.4 psbK-psbI_p4 psbK-psbI 4 TTGGCAAGCTGCTGTAAGTT TTTTTACTCCTCACGCCCAG 20 20 45.0 50.0 58.3 57.8 1.4 0.0 0.487716 7272 7670 541-541 1.000000 77.4 psbK-psbI_p5 psbK-psbI 5 TTTGGCAAGCTGCTGTAAGT TTTTTACTCCTCACGCCCAG 20 20 45.0 50.0 58.3 57.8 1.4 0.0 0.487716 7272 7670 542-542 1.000000 77.4 trnS-GCU-psbD_p1 trnS-GCU-psbD 1 ATTAGCAATCCGCCGCTTTA GCCGGACCATCCTACAAAAA 20 20 45.0 50.0 58.0 58.2 0.0 5.0 0.209227 7991 8973 1127-1128 1.000000 78.6 trnS-GCU-psbD_p2 trnS-GCU-psbD 2 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 20 20 45.0 45.0 58.0 58.2 0.0 0.0 0.284276 7991 8973 1114-1115 1.000000 78.0 trnS-GCU-psbD_p3 trnS-GCU-psbD 3 GCTTTAGTCCACTCAGCCAT GCCGGACCATCCTACAAAAA 20 20 50.0 50.0 57.6 58.2 0.9 5.0 0.580969 7991 8973 1113-1114 1.000000 75.6 trnS-GCU-psbD_p4 trnS-GCU-psbD 4 ATTAGCAATCCGCCGCTTTA GAAACGGTCCCTTCGTAACC 20 20 45.0 55.0 58.0 58.6 0.0 0.0 0.605692 7991 8973 1106-1107 1.000000 75.4 trnS-GCU-psbD_p5 trnS-GCU-psbD 5 GCTTTAGTCCACTCAGCCAT ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 57.6 58.2 0.9 0.0 0.656018 7991 8973 1100-1101 1.000000 75.0 trnG-UCC-trnT-GGU_p1 trnG-UCC-trnT-GGU 1 TGCCCCTTTGGTCTTTTGTT CGTGAAGAGAGTTGACACACT 20 21 45.0 47.6 58.1 57.9 0.0 0.0 1.212670 15452 15601 248-248 0.666667 57.0 trnG-UCC-trnT-GGU_p2 trnG-UCC-trnT-GGU 2 TTGCCCCTTTGGTCTTTTGT CGTGAAGAGAGTTGACACACT 20 21 45.0 47.6 58.1 57.9 0.0 0.0 1.212670 15452 15601 249-249 0.666667 57.0 trnG-UCC-trnT-GGU_p3 trnG-UCC-trnT-GGU 3 GCTCTTATTGCCCCTTTGGT CGTGAAGAGAGTTGACACACT 20 21 50.0 47.6 58.1 57.9 0.0 0.0 1.232811 15452 15601 256-256 0.666667 56.8 trnG-UCC-trnT-GGU_p4 trnG-UCC-trnT-GGU 4 AACAGAAAACCCATAGCCGG CGTGAAGAGAGTTGACACACT 20 21 50.0 47.6 58.2 57.9 0.0 0.0 1.251255 15452 15601 831-852 0.666667 56.8 trnG-UCC-trnT-GGU_p5 trnG-UCC-trnT-GGU 5 TTTTTGTTGGGCAGGCAGTA CGTGAAGAGAGTTGACACACT 20 21 45.0 47.6 58.2 57.9 0.0 0.0 1.303657 15452 15601 778-799 0.666667 56.3 rpoC2_p1 rpoC2 1 CTGGTCGAATTAGGGGAAGC TCGATTCAACAAGGTGCCAA 20 20 55.0 45.0 58.0 58.0 0.0 0.0 0.070808 28566 28685 2124-2268 1.000000 84.3 rpoC2_p2 rpoC2 2 CTGGTCGAATTAGGGGAAGC TTCGATTCAACAAGGTGCCA 20 20 55.0 45.0 58.0 58.0 0.0 0.0 0.070808 28566 28685 2125-2269 1.000000 84.3 rpoC2_p3 rpoC2 3 CAACCATTTCGAGCACAACC TCGATTCAACAAGGTGCCAA 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.076212 28566 28685 2229-2373 1.000000 84.3 rpoC2_p4 rpoC2 4 CAACCATTTCGAGCACAACC TTCGATTCAACAAGGTGCCA 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.076212 28566 28685 2230-2374 1.000000 84.3 rpoC2_p5 rpoC2 5 TACGCGAGGGACTTTCTTTG TCGATTCAACAAGGTGCCAA 20 20 50.0 45.0 57.9 58.0 15.9 0.0 0.100282 28566 28685 2564-2708 1.000000 84.0 rbcL_p1 rbcL 1 AGTAGACCCTGTCGTTGTGA AGCCGAGTTTAATTGCAATCA 20 21 50.0 38.1 58.0 56.5 0.0 13.4 2.500953 56579 58012 1608-1608 1.000000 60.1 rbcL_p2 rbcL 2 ATCGAGTAGACCCTGTCGTT AGCCGAGTTTAATTGCAATCA 20 21 50.0 38.1 57.9 56.5 0.0 13.4 2.617847 56579 58012 1612-1612 1.000000 59.2 rbcL_p3 rbcL 3 TAGACCCTGTCGTTGTGAGA AGCCGAGTTTAATTGCAATCA 20 21 50.0 38.1 57.7 56.5 0.0 13.4 2.773613 56579 58012 1606-1606 1.000000 58.0 rbcL_p4 rbcL 4 AGTAGACCCTGTCGTTGTGA AGCCGAGTTTAATTGCAATCAA 20 22 50.0 36.4 58.0 57.2 0.0 13.4 2.790898 56579 58012 1608-1608 1.000000 57.8 rbcL_p5 rbcL 5 GTAGACCCTGTCGTTGTGAG AGCCGAGTTTAATTGCAATCA 20 21 55.0 38.1 57.7 56.5 0.0 13.4 2.841371 56579 58012 1607-1607 1.000000 57.4 rbcL-psaI_p1 rbcL-psaI 1 CGCAGCTTGTGAAGTATGGA CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.3 58.0 0.0 26.9 0.301363 58013 59265 1383-1390 1.000000 78.8 rbcL-psaI_p2 rbcL-psaI 2 CGCAGCTTGTGAAGTATGGA AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 58.3 57.9 0.0 26.8 0.377897 58013 59265 1389-1396 1.000000 78.2 rbcL-psaI_p3 rbcL-psaI 3 GGCGATCAAATTCGAGTTCG CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 57.6 58.0 0.0 26.9 0.380261 58013 59265 1362-1369 1.000000 78.2 rbcL-psaI_p4 rbcL-psaI 4 TGGAAGGCGATCAAATTCGA CCATTGCAATTGCCGGAAAT 20 20 45.0 45.0 57.6 58.0 0.0 26.9 0.421933 58013 59265 1367-1374 1.000000 77.8 rbcL-psaI_p5 rbcL-psaI 5 GGCGATCAAATTCGAGTTCG AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 57.6 57.9 0.0 26.8 0.456795 58013 59265 1368-1375 1.000000 77.6 petA_p1 petA 1 ATTCTGGGATCTGCGATTGG GCCAATAAATCGCGGCTTTT 20 20 50.0 45.0 57.7 58.1 0.0 6.1 0.345931 61651 62613 1035-1035 1.000000 77.5 petA_p2 petA 2 ATTCTGGGATCTGCGATTGG CAATCGCCAATAAATCGCGG 20 20 50.0 50.0 57.7 58.3 0.0 6.1 0.593414 61651 62613 1040-1040 1.000000 75.5 petA_p3 petA 3 ATTCTGGGATCTGCGATTGG GATGGTAAGAAATCCCGGGC 20 20 50.0 55.0 57.7 58.4 0.0 18.5 0.661422 61651 62613 1006-1006 1.000000 74.9 petA_p4 petA 4 ATTCTGGGATCTGCGATTGG AGCAATCGCCAATAAATCGC 20 20 50.0 45.0 57.7 57.6 0.0 0.0 0.673066 61651 62613 1042-1042 1.000000 74.8 petA_p5 petA 5 TTCTGGGATCTGCGATTGGA GCCAATAAATCGCGGCTTTT 20 20 50.0 45.0 58.8 58.1 0.0 6.1 0.875519 61651 62613 1034-1034 1.000000 73.2 petA-psbJ_p1 petA-psbJ 1 GCGTCCAAGGCCTTTTATTC TCCTCTTTGGCTGATAGGTACT 20 22 50.0 45.5 57.4 58.0 17.6 0.0 2.566098 62614 63597 1194-1195 1.000000 60.1 petA-psbJ_p2 petA-psbJ 2 GCGTCCAAGGCCTTTTATTC CCTCTTTGGCTGATAGGTACTG 20 22 50.0 50.0 57.4 57.9 17.6 0.0 2.627055 62614 63597 1193-1194 1.000000 59.6 petA-psbJ_p3 petA-psbJ 3 GCGTCCAAGGCCTTTTATTC TTCCTCTTTGGCTGATAGGT 20 20 50.0 45.0 57.4 55.8 17.6 0.0 2.739592 62614 63597 1195-1196 1.000000 58.7 petA-psbJ_p4 petA-psbJ 4 GCGTCCAAGGCCTTTTATTC ACGGTTCATATTCTGGATTGGG 20 22 50.0 45.5 57.4 58.2 17.6 0.0 2.741176 62614 63597 1129-1130 1.000000 58.7 petA-psbJ_p5 petA-psbJ 5 GCGTCCAAGGCCTTTTATTC CGGTTCATATTCTGGATTGGGT 20 22 50.0 45.5 57.4 58.2 17.6 0.0 2.741176 62614 63597 1128-1129 1.000000 58.7 petG-trnW-CCA_p1 petG-trnW-CCA 1 GGGATTATTCGTGACTGCGT AGCGCTCTTAGTTCAGTTCG 20 20 50.0 50.0 58.1 58.0 0.0 2.6 0.080095 65982 66103 255-255 1.000000 82.2 petG-trnW-CCA_p2 petG-trnW-CCA 2 GGGATTATTCGTGACTGCGT CAAAACCCGATGTCGTAGGT 20 20 50.0 50.0 58.1 57.9 0.0 1.4 0.153017 65982 66103 219-219 1.000000 82.1 petG-trnW-CCA_p3 petG-trnW-CCA 3 AGCGGGATTATTCGTGACTG AGCGCTCTTAGTTCAGTTCG 20 20 50.0 50.0 57.8 58.0 0.0 2.6 0.241096 65982 66103 258-258 1.000000 80.9 petG-trnW-CCA_p4 petG-trnW-CCA 4 GCGTATTTGCAATACAGGCG AGCGCTCTTAGTTCAGTTCG 20 20 50.0 50.0 58.3 58.0 0.0 2.6 0.274035 65982 66103 239-239 1.000000 80.8 petG-trnW-CCA_p5 petG-trnW-CCA 5 AGCGGGATTATTCGTGACTG CAAAACCCGATGTCGTAGGT 20 20 50.0 50.0 57.8 57.9 0.0 1.4 0.314018 65982 66103 222-222 1.000000 80.7 trnP-GGG-psaJ_p1 trnP-GGG-psaJ 1 AAAACAAACGCGCTACCAAG AAACCTGCTAAAGCCCCAAA 20 20 45.0 45.0 57.9 57.9 5.7 0.0 0.209515 66383 66772 498-498 1.000000 79.0 trnP-GGG-psaJ_p2 trnP-GGG-psaJ 2 CAAAACAAACGCGCTACCAA AAACCTGCTAAAGCCCCAAA 20 20 45.0 45.0 58.2 57.9 5.7 0.0 0.266772 66383 66772 499-499 1.000000 78.5 trnP-GGG-psaJ_p3 trnP-GGG-psaJ 3 TTGTACCCAAAACAAACGCG AAACCTGCTAAAGCCCCAAA 20 20 45.0 45.0 57.8 57.9 3.5 0.0 0.285929 66383 66772 506-506 1.000000 78.3 trnP-GGG-psaJ_p4 trnP-GGG-psaJ 4 TACCCAAAACAAACGCGCTA AAACCTGCTAAAGCCCCAAA 20 20 45.0 45.0 58.4 57.9 5.7 0.0 0.504672 66383 66772 503-503 1.000000 76.6 trnP-GGG-psaJ_p5 trnP-GGG-psaJ 5 AAAACAAACGCGCTACCAAG CTGCTAAAGCCCCAAACCAT 20 20 45.0 50.0 57.9 58.4 5.7 0.0 0.553406 66383 66772 494-494 1.000000 76.2 petB_p1 petB 1 GGTAGTTCGACCGTGGAATT ACCACAACCTACCCGATATGA 20 21 50.0 47.6 57.6 58.2 0.0 0.0 1.620707 73447 74920 1581-1594 1.000000 67.3 petB_p2 petB 2 GGTAGTTCGACCGTGGAATT ACCACAACCTACCCGATATG 20 20 50.0 50.0 57.6 56.7 0.0 0.0 1.668360 73447 74920 1581-1594 1.000000 67.0 petB_p3 petB 3 GGTAGTTCGACCGTGGAATT TGAAATACCACAACCTACCCG 20 21 50.0 47.6 57.6 57.7 0.0 0.0 1.712867 73447 74920 1587-1600 1.000000 66.6 petB_p4 petB 4 ATTCTGGTAGTTCGACCGTG ACCACAACCTACCCGATATGA 20 21 50.0 47.6 57.4 58.2 0.0 0.0 1.894865 73447 74920 1586-1599 1.000000 65.1 petB_p5 petB 5 CATTCTGGTAGTTCGACCGT ACCACAACCTACCCGATATGA 20 21 50.0 47.6 57.4 58.2 0.0 0.0 1.894865 73447 74920 1587-1600 1.000000 65.1 infA_p1 infA 1 CGCAAACCAATACGCACTTT TTAAAGCGTATCAGCCGACC 20 20 45.0 50.0 58.0 58.1 0.0 0.0 0.114321 78461 78802 1074-1075 1.000000 79.8 infA_p2 infA 2 TCCACTACCAGCACCCTTTA TTAAAGCGTATCAGCCGACC 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.102397 78461 78802 1336-1337 1.000000 79.7 infA_p3 infA 3 TTGCAAACCCACTGTACGAA TTAAAGCGTATCAGCCGACC 20 20 45.0 50.0 58.0 58.1 0.0 0.0 0.109251 78461 78802 1300-1301 1.000000 79.7 infA_p4 infA 4 GTAATGCGGCATCTCTTCCA TTAAAGCGTATCAGCCGACC 20 20 50.0 50.0 58.0 58.1 17.0 0.0 0.115874 78461 78802 1352-1353 1.000000 79.6 infA_p5 infA 5 TAATGCGGCATCTCTTCCAC TTAAAGCGTATCAGCCGACC 20 20 50.0 50.0 58.0 58.1 17.0 0.0 0.115874 78461 78802 1351-1352 1.000000 79.6 infA-rps8_p1 infA-rps8 1 TGACTTTTGCTTCTCTCGGG TTTTGGGCGGAATGGGAATT 20 20 50.0 45.0 57.8 58.3 0.0 0.0 0.519304 78803 78914 276-277 1.000000 78.2 infA-rps8_p2 infA-rps8 2 GACTTTTGCTTCTCTCGGGT TTTTGGGCGGAATGGGAATT 20 20 50.0 45.0 57.8 58.3 0.0 0.0 0.520813 78803 78914 275-276 1.000000 78.2 infA-rps8_p3 infA-rps8 3 TGACTTTTGCTTCTCTCGGG GGCGGAATGGGAATTGCTAT 20 20 50.0 50.0 57.8 58.4 0.0 0.0 0.549010 78803 78914 271-272 1.000000 78.0 infA-rps8_p4 infA-rps8 4 GACTTTTGCTTCTCTCGGGT GGCGGAATGGGAATTGCTAT 20 20 50.0 50.0 57.8 58.4 0.0 0.0 0.550518 78803 78914 270-271 1.000000 78.0 infA-rps8_p5 infA-rps8 5 GGGTAGGGCTTCCGTAACTA TTTTGGGCGGAATGGGAATT 20 20 55.0 45.0 58.2 58.3 1.4 0.0 0.568969 78803 78914 308-309 1.000000 77.5 rpl14-rpl16_p1 rpl14-rpl16 1 AGCAGCTCCTATGACTCGAA TAGCTGCCAGTAAAATGCCC 20 20 50.0 50.0 58.2 58.2 0.0 0.0 0.468267 79840 79948 252-252 1.000000 77.8 rpl14-rpl16_p2 rpl14-rpl16 2 CGAGCTCCACTGTTATCTGC TAGCTGCCAGTAAAATGCCC 20 20 55.0 50.0 58.4 58.2 8.5 0.0 0.671988 79840 79948 218-218 1.000000 76.5 rpl14-rpl16_p3 rpl14-rpl16 3 TAGCAGCTCCTATGACTCGA TAGCTGCCAGTAAAATGCCC 20 20 50.0 50.0 57.4 58.2 0.0 0.0 0.877608 79840 79948 253-253 1.000000 74.6 rpl14-rpl16_p4 rpl14-rpl16 4 AGCAGCTCCTATGACTCGAA GCCAGTAAAATGCCCATACG 20 20 50.0 50.0 58.2 57.2 0.0 0.0 1.005413 79840 79948 247-247 1.000000 73.6 rpl14-rpl16_p5 rpl14-rpl16 5 GCAGCTCCTATGACTCGAAT TAGCTGCCAGTAAAATGCCC 20 20 50.0 50.0 57.2 58.2 0.0 0.0 1.047732 79840 79948 251-251 1.000000 73.2 ndhF-rpl32_p1 ndhF-rpl32 1 AGACAAAATACTGGAATTCTTCA TGACATCGAAGTACGTTTTT 23 20 30.4 35.0 53.9 53.2 5.2 0.0 11.837280 107114 107882 824-827 1.000000 41.1 ndhF-rpl32_p2 ndhF-rpl32 2 AGACAAAATACTGGAATTCTTCA TTGACATCGAAGTACGTTTT 23 20 30.4 35.0 53.9 53.2 5.2 0.0 11.837280 107114 107882 825-828 1.000000 41.1 ndhF-rpl32_p3 ndhF-rpl32 3 AGACAAAATACTGGAATTCTTCA TTTGACATCGAAGTACGTTT 23 20 30.4 35.0 53.9 53.2 5.2 0.0 11.837280 107114 107882 826-829 1.000000 41.1 ndhF-rpl32_p4 ndhF-rpl32 4 AGACAAAATACTGGAATTCTTCA TTTTGACATCGAAGTACGTT 23 20 30.4 35.0 53.9 53.2 5.2 0.0 11.837280 107114 107882 827-830 1.000000 41.1 ndhF-rpl32_p5 ndhF-rpl32 5 AGACAAAATACTGGAATTCTTCA TCCAAAGATTTTTACGAATACGCT 23 24 30.4 33.3 53.9 57.3 5.2 0.0 11.801485 107114 107882 852-855 1.000000 41.0 ndhH_p1 ndhH 1 TCCCAGTAGGAGGGTCAAAA TTGATGAGCCTCGTTTTGAG 20 20 50.0 45.0 57.6 56.1 0.0 0.0 2.356404 116557 117738 1370-1370 1.000000 61.4 ndhH_p2 ndhH 2 TCCCAGTAGGAGGGTCAAAA TTTGATGAGCCTCGTTTTGAG 20 21 50.0 42.9 57.6 56.8 0.0 0.0 2.602069 116557 117738 1371-1371 1.000000 59.4 ndhH_p3 ndhH 3 TCCCAGTAGGAGGGTCAAAA TTTGATGAGCCTCGTTTTGA 20 20 50.0 40.0 57.6 55.6 0.0 0.0 2.837133 116557 117738 1371-1371 1.000000 57.5 ndhH_p4 ndhH 4 TCCCAGTAGGAGGGTCAAAA TTTTGATGAGCCTCGTTTTGAG 20 22 50.0 40.9 57.6 57.5 0.0 0.0 2.914557 116557 117738 1372-1372 1.000000 56.9 ndhH_p5 ndhH 5 TCCCAGTAGGAGGGTCAAAAT TTGATGAGCCTCGTTTTGAG 21 20 47.6 45.0 58.1 56.1 0.0 0.0 3.000672 116557 117738 1370-1370 1.000000 56.2