primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	ATAGACCAGACCAACCCACA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.0	58.1	0.0	7.6	0.102930	19716	20853	1280-1470	1.000000	84.5
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	ATAGACCAGACCAACCCACA	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.125085	19716	20853	1283-1473	1.000000	84.3
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	TAGACCAGACCAACCCACAA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.2	58.1	0.0	7.6	0.254934	19716	20853	1279-1469	1.000000	83.2
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	TAGACCAGACCAACCCACAA	TCAGTGGTAGAGTAACGCCA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.277089	19716	20853	1282-1472	1.000000	83.1
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	ACAAAACGGTCTCTGCGTAG	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.2	58.1	0.0	7.6	0.284498	19716	20853	1257-1447	1.000000	83.0
atpF-atpA_p1	atpF-atpA	1	GCCTGCTTAGAGCCATGAAA	AGCAATGCCATCTCCTACTTG	20	21	50.0	47.6	58.2	58.1	0.0	0.0	1.316487	38841	38933	222-268	1.000000	77.3
atpF-atpA_p2	atpF-atpA	2	GCCTGCTTAGAGCCATGAAA	GCAATGCCATCTCCTACTTGA	20	21	50.0	47.6	58.2	58.1	0.0	0.0	1.321700	38841	38933	221-267	1.000000	77.3
atpF-atpA_p3	atpF-atpA	3	AATATCGGCCTGCTTAGAGC	AGCAATGCCATCTCCTACTTG	20	21	50.0	47.6	57.5	58.1	0.0	0.0	1.535823	38841	38933	229-275	1.000000	75.5
atpF-atpA_p4	atpF-atpA	4	AATATCGGCCTGCTTAGAGC	GCAATGCCATCTCCTACTTGA	20	21	50.0	47.6	57.5	58.1	0.0	0.0	1.541037	38841	38933	228-274	1.000000	75.5
atpF-atpA_p5	atpF-atpA	5	GCCTGCTTAGAGCCATGAAA	AGCAATGCCATCTCCTACTT	20	20	50.0	45.0	58.2	56.6	0.0	0.0	1.658745	38841	38933	222-268	1.000000	74.6
psbI-psbK_p1	psbI-psbK	1	TCTTTACGCCCAGGATTTCG	CAAGCTGCCGTAAGTTTTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.068070	41708	42351	751-790	1.000000	84.7
psbI-psbK_p2	psbI-psbK	2	TCGTCCTGGATCGTTCGATA	CAAGCTGCCGTAAGTTTTCG	20	20	50.0	50.0	58.0	58.0	7.7	0.0	0.091860	41708	42351	734-773	1.000000	84.6
psbI-psbK_p3	psbI-psbK	3	TCTTTACGCCCAGGATTTCG	AGCTGCCGTAAGTTTTCGAT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.113304	41708	42351	749-788	1.000000	84.4
psbI-psbK_p4	psbI-psbK	4	ATTTCGTCCTGGATCGTTCG	CAAGCTGCCGTAAGTTTTCG	20	20	50.0	50.0	58.1	58.0	15.0	0.0	0.126958	41708	42351	737-776	1.000000	84.3
psbI-psbK_p5	psbI-psbK	5	TCTTTACGCCCAGGATTTCG	GCCCGAGGCTTATGCTATTT	20	20	50.0	50.0	58.0	58.1	0.0	7.6	0.120982	41708	42351	836-875	1.000000	83.8
trnV-UAC_p1	trnV-UAC	1	AGGCGGAATATAAGAGTTTCC	TGCATGTTGGGTTCTTGGAA	21	20	42.9	45.0	55.2	57.9	0.0	0.0	3.907268	47161	47197	119-132	1.000000	56.5
trnV-UAC_p2	trnV-UAC	2	AGGCGGAATATAAGAGTTTCC	AATGAAAGAGCCCAATGCCA	21	20	42.9	45.0	55.2	58.1	0.0	0.0	3.886721	47161	47197	145-158	1.000000	56.0
trnV-UAC_p3	trnV-UAC	3	AGAGACAATAAGAAATAGGCGGA	TGCATGTTGGGTTCTTGGAA	23	20	39.1	45.0	57.1	57.9	0.0	0.0	3.950566	47161	47197	135-148	1.000000	55.8
trnV-UAC_p4	trnV-UAC	4	AGAGACAATAAGAAATAGGCGGA	AATGAAAGAGCCCAATGCCA	23	20	39.1	45.0	57.1	58.1	0.0	0.0	3.930019	47161	47197	161-174	1.000000	55.2
trnV-UAC_p5	trnV-UAC	5	AGGCGGAATATAAGAGTTTCC	ATGAAAGAGCCCAATGCCAT	21	20	42.9	45.0	55.2	57.8	0.0	0.0	3.998111	47161	47197	144-157	1.000000	55.2
atpE-rbcL_p1	atpE-rbcL	1	AGAACACGACTAGAGGCTGT	GCTTTGAATCCGACACTTGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.118480	50094	50601	528-603	1.000000	86.2
atpE-rbcL_p2	atpE-rbcL	2	ATCTTGGACGAGTTGAAGGC	GCTTTGAATCCGACACTTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.131501	50094	50601	590-665	1.000000	85.9
atpE-rbcL_p3	atpE-rbcL	3	GGACGAGTTGAAGGCAAGAA	GCTTTGAATCCGACACTTGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.151944	50094	50601	585-660	1.000000	85.8
atpE-rbcL_p4	atpE-rbcL	4	AGAACACGACTAGAGGCTGT	CCAGCTTTGAATCCGACACT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.274070	50094	50601	531-606	1.000000	84.9
atpE-rbcL_p5	atpE-rbcL	5	ATCTTGGACGAGTTGAAGGC	CCAGCTTTGAATCCGACACT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.287091	50094	50601	593-668	1.000000	84.6
accD_p1	accD	1	ACGATGAAATAAAGGAGACAAGT	TCTGATATTAGTTGTGCGCCT	23	21	34.8	42.9	55.9	57.2	0.0	4.2	6.874306	52500	54710	2298-2412	1.000000	48.2
accD_p2	accD	2	ACGATGAAATAAAGGAGACAAGT	GTCTGATATTAGTTGTGCGCC	23	21	34.8	47.6	55.9	57.1	0.0	4.2	6.916312	52500	54710	2299-2413	1.000000	48.2
accD_p3	accD	3	AACGATGAAATAAAGGAGACAAGT	TCTGATATTAGTTGTGCGCCT	24	21	33.3	42.9	56.6	57.2	0.0	4.2	7.192536	52500	54710	2299-2413	1.000000	48.2
accD_p4	accD	4	AACGATGAAATAAAGGAGACAAGT	GTCTGATATTAGTTGTGCGCC	24	21	33.3	47.6	56.6	57.1	0.0	4.2	7.234541	52500	54710	2300-2414	1.000000	48.2
accD_p5	accD	5	ACGATGAAATAAAGGAGACAAGT	TCTGATATTAGTTGTGCGCC	23	20	34.8	45.0	55.9	55.6	0.0	4.2	7.409789	52500	54710	2298-2412	1.000000	48.2
accD-infA_p1	accD-infA	1	TTCTCGTATGTGCTTCTGGC	ACAAATGACCAGAAGGCGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.028889	54711	56590	2511-2710	1.000000	89.6
accD-infA_p2	accD-infA	2	GTATGGGCTCGGTAGTAGGT	AACAAATGACCAGAAGGCGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.026206	54711	56590	2584-2783	1.000000	89.5
accD-infA_p3	accD-infA	3	GTATGGGCTCGGTAGTAGGT	ACAAATGACCAGAAGGCGTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.026206	54711	56590	2583-2782	1.000000	89.5
accD-infA_p4	accD-infA	4	TATGGGCTCGGTAGTAGGTG	AACAAATGACCAGAAGGCGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.027622	54711	56590	2583-2782	1.000000	89.5
accD-infA_p5	accD-infA	5	TATGGGCTCGGTAGTAGGTG	ACAAATGACCAGAAGGCGTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.027622	54711	56590	2582-2781	1.000000	89.5
psbE-petL_p1	psbE-petL	1	TTCTCCCGTATTCCCAGACA	AGGACTAAGGTTAAAATAAGCAGA	20	24	50.0	33.3	57.8	55.5	0.0	2.6	6.713834	69281	70555	1296-1380	1.000000	47.2
psbE-petL_p2	psbE-petL	2	TTCTCCCGTATTCCCAGACA	GGACTAAGGTTAAAATAAGCAGAACT	20	26	50.0	34.6	57.8	57.4	0.0	2.6	6.804403	69281	70555	1295-1379	1.000000	47.2
psbE-petL_p3	psbE-petL	3	ATAATGTCAGCAAAAGCGCG	AGGACTAAGGTTAAAATAAGCAGA	20	24	45.0	33.3	57.8	55.5	0.0	2.6	6.651027	69281	70555	1316-1400	1.000000	47.1
psbE-petL_p4	psbE-petL	4	ATAATGTCAGCAAAAGCGCG	AGGACTAAGGTTAAAATAAGCAGAAC	20	26	45.0	34.6	57.8	57.4	0.0	2.6	6.741597	69281	70555	1316-1400	1.000000	47.1
psbE-petL_p5	psbE-petL	5	ATAATGTCAGCAAAAGCGCG	GGACTAAGGTTAAAATAAGCAGAACT	20	26	45.0	34.6	57.8	57.4	0.0	2.6	6.741597	69281	70555	1315-1399	1.000000	47.1
ycf2_p1	ycf2	1	CTCCTTCCGCATGTGCTTAT	GATTCGATATCCCTCGGTGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.043289	83455	83574	2264-2612	1.000000	88.7
ycf2_p2	ycf2	2	TCATCAGATCCCGGATCGAA	GATTCGATATCCCTCGGTGC	20	20	50.0	55.0	58.0	58.0	19.7	0.0	0.006955	83455	83574	1661-2015	0.818182	81.9
ycf2_p3	ycf2	3	TTCATCAGATCCCGGATCGA	GATTCGATATCCCTCGGTGC	20	20	50.0	55.0	58.0	58.0	19.7	0.0	0.006955	83455	83574	1662-2016	0.818182	81.9
ycf2_p4	ycf2	4	TCATCAGATCCCGGATCGAA	GCAAAATGGGTCGATGGAATC	20	21	50.0	47.6	58.0	57.9	19.7	0.0	1.088694	83455	83574	1021-1468	0.636364	65.7
ycf2_p5	ycf2	5	TTCATCAGATCCCGGATCGA	GCAAAATGGGTCGATGGAATC	20	21	50.0	47.6	58.0	57.9	19.7	0.0	1.088694	83455	83574	1022-1469	0.636364	65.7
trnV-GAC-CD863_pgr004_p1	trnV-GAC-CD863_pgr004	1	CCGAATGAGGTTAGGGAAGG	AAACACCACTTCACGTTCGA	20	20	55.0	45.0	57.4	58.0	0.0	0.0	0.635001	94691	95187	580-703	1.000000	84.2
trnV-GAC-CD863_pgr004_p2	trnV-GAC-CD863_pgr004	2	CGAATGAGGTTAGGGAAGGG	AAACACCACTTCACGTTCGA	20	20	55.0	45.0	57.4	58.0	0.0	0.0	0.635001	94691	95187	579-702	1.000000	84.2
trnV-GAC-CD863_pgr004_p3	trnV-GAC-CD863_pgr004	3	CCGAATGAGGTTAGGGAAGG	CCACTGGAAACACCACTTCA	20	20	55.0	50.0	57.4	57.9	0.0	0.0	0.673398	94691	95187	587-710	1.000000	83.8
trnV-GAC-CD863_pgr004_p4	trnV-GAC-CD863_pgr004	4	CGAATGAGGTTAGGGAAGGG	CCACTGGAAACACCACTTCA	20	20	55.0	50.0	57.4	57.9	0.0	0.0	0.673398	94691	95187	586-709	1.000000	83.8
trnV-GAC-CD863_pgr004_p5	trnV-GAC-CD863_pgr004	5	CGAATGAGGTTAGGGAAGGG	TGGAAACACCACTTCACGTT	20	20	55.0	45.0	57.4	57.9	0.0	0.0	0.738891	94691	95187	582-705	1.000000	83.3
CD863_pgr004-CD863_pgr003_p1	CD863_pgr004-CD863_pgr003	1	GACCGGAGTGAAGTCGTAAC	GTCTCTGGGTGCCTAGGTAT	20	20	55.0	55.0	58.0	57.9	9.5	11.3	0.115114	96702	98941	1748-2348	1.000000	91.1
CD863_pgr004-CD863_pgr003_p2	CD863_pgr004-CD863_pgr003	2	GACCGGAGTGAAGTCGTAAC	ATGCTCAATTTCCACTCCCC	20	20	55.0	50.0	58.0	57.9	9.5	0.0	0.175486	96702	98941	1800-2400	1.000000	90.6
CD863_pgr004-CD863_pgr003_p3	CD863_pgr004-CD863_pgr003	3	GACCGGAGTGAAGTCGTAAC	CACCGTAAGCCCTTCCATTT	20	20	55.0	50.0	58.0	58.2	9.5	0.0	0.191326	96702	98941	1727-2327	1.000000	90.5
CD863_pgr004-CD863_pgr003_p4	CD863_pgr004-CD863_pgr003	4	GACCGGAGTGAAGTCGTAAC	AAGCATTTCGTCGCTTACCA	20	20	55.0	45.0	58.0	58.2	9.5	0.0	0.222179	96702	98941	1779-2379	1.000000	90.2
CD863_pgr004-CD863_pgr003_p5	CD863_pgr004-CD863_pgr003	5	GACCGGAGTGAAGTCGTAAC	GTATCCACCGTAAGCCCTTC	20	20	55.0	55.0	58.0	57.8	9.5	0.0	0.261358	96702	98941	1732-2332	1.000000	89.9
CD863_pgr002-CD863_pgr001_p1	CD863_pgr002-CD863_pgr001	1	GCTCTCCATAAGGTCAAGGC	AGTATCGTCACCGCAGTAGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.073688	101889	102103	371-561	1.000000	86.8
CD863_pgr002-CD863_pgr001_p2	CD863_pgr002-CD863_pgr001	2	GCTCTCCATAAGGTCAAGGC	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.073688	101889	102103	369-559	1.000000	86.8
CD863_pgr002-CD863_pgr001_p3	CD863_pgr002-CD863_pgr001	3	GCTCTCCATAAGGTCAAGGC	GCATCGAGCTATTTTTCCGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.063759	101889	102103	405-595	1.000000	86.7
CD863_pgr002-CD863_pgr001_p4	CD863_pgr002-CD863_pgr001	4	CCATAAGGTCAAGGCGAGAC	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.1	58.0	4.1	0.0	0.092779	101889	102103	364-554	1.000000	86.7
CD863_pgr002-CD863_pgr001_p5	CD863_pgr002-CD863_pgr001	5	CCATAAGGTCAAGGCGAGAC	GCATCGAGCTATTTTTCCGC	20	20	55.0	50.0	58.1	58.0	4.1	0.0	0.082851	101889	102103	400-590	1.000000	86.5
ndhF-rpl32_p1	ndhF-rpl32	1	GAAAGCGGAAAGAAAGGCAC	GCCCGATATGCTTTTCCCTT	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.359468	105737	106547	866-956	1.000000	82.6
ndhF-rpl32_p2	ndhF-rpl32	2	GAAAGCGGAAAGAAAGGCAC	CTTCCAAACATTGCTGCGAA	20	20	50.0	45.0	57.9	57.6	0.0	0.0	0.472507	105737	106547	849-939	1.000000	81.7
ndhF-rpl32_p3	ndhF-rpl32	3	GAAAGCGGAAAGAAAGGCAC	TACGCTTCTTTGGAACTGCC	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.532470	105737	106547	808-898	1.000000	81.3
ndhF-rpl32_p4	ndhF-rpl32	4	AAAGAAAGGCACGATCCGAG	GCCCGATATGCTTTTCCCTT	20	20	50.0	50.0	58.3	58.3	0.0	0.0	0.580736	105737	106547	858-948	1.000000	80.8
ndhF-rpl32_p5	ndhF-rpl32	5	GAAAGAAAGGCACGATCCGA	GCCCGATATGCTTTTCCCTT	20	20	50.0	50.0	58.3	58.3	0.0	0.0	0.585578	105737	106547	859-949	1.000000	80.8
ycf1-chlN_p1	ycf1-chlN	1	GCCATTGTATGAGGCTTCCA	TGGAATGGCTCATGCTAACC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.114275	124500	125583	1389-1655	1.000000	89.6
ycf1-chlN_p2	ycf1-chlN	2	GGCGGTACTTAGCAGAAACA	TGGAATGGCTCATGCTAACC	20	20	50.0	50.0	57.9	57.9	9.8	0.0	0.146087	124500	125583	1630-1896	1.000000	88.7
ycf1-chlN_p3	ycf1-chlN	3	GCCATTGTATGAGGCTTCCA	TGTTGAAACCCAAGGTCTGG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.123036	124500	125583	1716-1982	1.000000	88.4
ycf1-chlN_p4	ycf1-chlN	4	GCCATTGTATGAGGCTTCCA	GGTGTTGAAACCCAAGGTCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.124503	124500	125583	1718-1984	1.000000	88.4
ycf1-chlN_p5	ycf1-chlN	5	GGCGGTACTTAGCAGAAACA	TGTTGAAACCCAAGGTCTGG	20	20	50.0	50.0	57.9	57.9	9.8	0.0	0.154848	124500	125583	1957-2223	1.000000	87.8
chlL-trnH-GUG_p1	chlL-trnH-GUG	1	TCAATGGAGCAGCAAAACCT	TGACCATTCAGCCATGGATG	20	20	45.0	50.0	58.0	57.9	0.0	24.3	0.061861	127852	128058	1008-1326	0.909091	80.7
chlL-trnH-GUG_p2	chlL-trnH-GUG	2	TCAATGGAGCAGCAAAACCT	TGCTTTGACCATTCAGCCAT	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.072864	127852	128058	1013-1331	0.909091	80.6
chlL-trnH-GUG_p3	chlL-trnH-GUG	3	TCAATGGAGCAGCAAAACCT	CCGCGACTTCCCAATTATGA	20	20	45.0	50.0	58.0	58.1	0.0	8.2	0.052248	127852	128058	1039-1254	0.818182	76.5
chlL-trnH-GUG_p4	chlL-trnH-GUG	4	TCAATGGAGCAGCAAAACCT	TTGGGTGCTTTGACCATTCA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.079521	127852	128058	1018-1233	0.818182	76.4
chlL-trnH-GUG_p5	chlL-trnH-GUG	5	TCAATGGAGCAGCAAAACCT	AGTTGGGTGCTTTGACCATT	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.086962	127852	128058	1020-1235	0.818182	76.3