primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbD-trnE-UUC_p1	psbD-trnE-UUC	1	AAGCAATAGACCGGACCAAC	CGTTGCCTCCTTGAAAGAGA	20	20	50.0	50.0	57.9	57.8	9.5	0.0	0.279257	20994	22505	1675-1675	1.000000	77.9
psbD-trnE-UUC_p2	psbD-trnE-UUC	2	ACAAAACGGTCTCTGCGTAG	CGTTGCCTCCTTGAAAGAGA	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.390263	20994	22505	1647-1647	1.000000	77.0
psbD-trnE-UUC_p3	psbD-trnE-UUC	3	AACAAAACGGTCTCTGCGTA	CGTTGCCTCCTTGAAAGAGA	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.397970	20994	22505	1648-1648	1.000000	77.0
psbD-trnE-UUC_p4	psbD-trnE-UUC	4	AAGCAATAGACCGGACCAAC	GAAAGAGAGATGTCCTGGGC	20	20	50.0	55.0	57.9	57.7	9.5	0.0	0.433393	20994	22505	1663-1663	1.000000	76.7
psbD-trnE-UUC_p5	psbD-trnE-UUC	5	ACAAAACGGTCTCTGCGTAG	GAAAGAGAGATGTCCTGGGC	20	20	50.0	55.0	58.2	57.7	0.0	0.0	0.544399	20994	22505	1635-1635	1.000000	75.8
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	TTAGAGTACCGCCCTGTCAA	GCTTGACTCGCCGTTTTTAC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.125992	23015	24751	1890-1891	1.000000	79.2
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	ATTGGTTAGAGTACCGCCCT	GCTTGACTCGCCGTTTTTAC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.177991	23015	24751	1895-1896	1.000000	78.7
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	TTAGAGTACCGCCCTGTCAA	CTTGACTCGCCGTTTTTACG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.279553	23015	24751	1889-1890	1.000000	77.9
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	ATTGGTTAGAGTACCGCCCT	CTTGACTCGCCGTTTTTACG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.331553	23015	24751	1894-1895	1.000000	77.5
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	TTAGAGTACCGCCCTGTCAA	CTGTACTTCGGCTTGACTCG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.453652	23015	24751	1900-1901	1.000000	76.5
petN-trnC-GCA_p1	petN-trnC-GCA	1	TAAAGCCCACTTCTACCCCA	GCGACATAGCCAAGTGGTAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.066311	25682	25992	470-470	1.000000	79.6
petN-trnC-GCA_p2	petN-trnC-GCA	2	ACTACCATTAGAGCAGCCCA	GCGACATAGCCAAGTGGTAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.138967	25682	25992	422-422	1.000000	79.1
petN-trnC-GCA_p3	petN-trnC-GCA	3	TAAAGCCCACTTCTACCCCA	AATCCTCGATCCCCAGTTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.168195	25682	25992	436-436	1.000000	78.8
petN-trnC-GCA_p4	petN-trnC-GCA	4	TAAAGCCCACTTCTACCCCA	ATCCTCGATCCCCAGTTCAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.168195	25682	25992	435-435	1.000000	78.8
petN-trnC-GCA_p5	petN-trnC-GCA	5	ACTACCATTAGAGCAGCCCA	ATCCTCGATCCCCAGTTCAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.240850	25682	25992	387-387	1.000000	78.3
atpI-atpH_p1	atpI-atpH	1	CGAATCCATGGAGGGTCATC	CCTACGGATAATCCAGCAGC	20	20	55.0	55.0	57.8	57.9	21.3	0.0	0.261932	38220	38874	730-733	1.000000	78.6
atpI-atpH_p2	atpI-atpH	2	CGAATCCATGGAGGGTCATC	GCCTACGGATAATCCAGCAG	20	20	55.0	55.0	57.8	57.9	21.3	0.0	0.261932	38220	38874	731-734	1.000000	78.6
atpI-atpH_p3	atpI-atpH	3	CGAATCCATGGAGGGTCATC	AACGGAAGCAGCAGAAATCA	20	20	55.0	45.0	57.8	58.1	21.3	0.0	0.279658	38220	38874	707-710	1.000000	78.4
atpI-atpH_p4	atpI-atpH	4	CGAATCCATGGAGGGTCATC	ACGGAAGCAGCAGAAATCAA	20	20	55.0	45.0	57.8	58.1	21.3	0.0	0.279658	38220	38874	706-709	1.000000	78.4
atpI-atpH_p5	atpI-atpH	5	CGAATCCATGGAGGGTCATC	GCCAGGTCCAATAGAAGCAA	20	20	55.0	50.0	57.8	57.9	21.3	0.0	0.304648	38220	38874	752-755	1.000000	78.2
rpl32-ndhF_p1	rpl32-ndhF	1	TTTTGCTGCGGCCCAATA	GGAAGGAAAGGCACAATCCA	18	20	50.0	50.0	57.9	58.1	16.6	0.0	2.180394	55515	56173	793-793	1.000000	62.7
rpl32-ndhF_p2	rpl32-ndhF	2	CTTTTGCTGCGGCCCAATA	GGAAGGAAAGGCACAATCCA	19	20	52.6	50.0	59.1	58.1	16.6	0.0	2.187190	55515	56173	794-794	1.000000	62.6
rpl32-ndhF_p3	rpl32-ndhF	3	TTTTGCTGCGGCCCAATA	AGGAGCCTAATCCGATTGGT	18	20	50.0	50.0	57.9	58.2	16.6	3.7	2.295935	55515	56173	828-828	1.000000	61.7
rpl32-ndhF_p4	rpl32-ndhF	4	CTTTTGCTGCGGCCCAATA	AGGAGCCTAATCCGATTGGT	19	20	52.6	50.0	59.1	58.2	16.6	3.7	2.302732	55515	56173	829-829	1.000000	61.7
rpl32-ndhF_p5	rpl32-ndhF	5	TTTTGCTGCGGCCCAATA	GGCGAACACTCTTAGTTGCT	18	20	50.0	50.0	57.9	58.2	16.6	0.0	2.306725	55515	56173	861-861	1.000000	61.6
ndhF-trnN-GUU_p1	ndhF-trnN-GUU	1	ATGAGATGCTTAGGAGGGGG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	58.3	57.7	0.0	0.0	0.571029	58391	59220	995-1003	1.000000	76.2
ndhF-trnN-GUU_p2	ndhF-trnN-GUU	2	GCTTAGGAGGGGGACGTATA	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	57.7	57.7	0.0	0.0	0.610105	58391	59220	988-996	1.000000	75.8
ndhF-trnN-GUU_p3	ndhF-trnN-GUU	3	ATGAGATGCTTAGGAGGGGG	CCGATTGGTCGTAGGTTCAA	20	20	55.0	50.0	58.3	57.6	0.0	0.0	0.633790	58391	59220	957-965	1.000000	75.7
ndhF-trnN-GUU_p4	ndhF-trnN-GUU	4	GCTTAGGAGGGGGACGTATA	CCGATTGGTCGTAGGTTCAA	20	20	55.0	50.0	57.7	57.6	0.0	0.0	0.672866	58391	59220	950-958	1.000000	75.4
ndhF-trnN-GUU_p5	ndhF-trnN-GUU	5	AGGAGAGGGAATGAGATGCT	TTCCTCAGTAGCTCAGTGGT	20	20	50.0	50.0	57.5	57.7	0.0	0.0	0.803878	58391	59220	1005-1013	1.000000	74.3
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	CCGCGACTTCCCAATTATGA	TAGACCACGGGTTCTCATGT	20	20	50.0	50.0	58.1	58.1	8.2	0.0	0.118874	73242	73951	702-790	1.000000	83.0
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	CCGCGACTTCCCAATTATGA	GACCACGGGTTCTCATGTTT	20	20	50.0	50.0	58.1	58.1	8.2	0.0	0.159686	73242	73951	700-788	1.000000	82.7
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	CCGCGACTTCCCAATTATGA	GTAGACCACGGGTTCTCATG	20	20	50.0	55.0	58.1	57.7	8.2	0.0	0.347149	73242	73951	703-791	1.000000	81.2
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	CCGCGACTTCCCAATTATGA	ACCACGGGTTCTCATGTTTT	20	20	50.0	45.0	58.1	57.6	8.2	0.0	0.410218	73242	73951	699-787	1.000000	80.7
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GGTGCTTTAACCATTCAGCC	TAGACCACGGGTTCTCATGT	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.721728	73242	73951	678-766	1.000000	78.2
ycf2_p1	ycf2	1	CCCAGCATTCATCAAGTTGG	TCCAAACCAACCCCAATGTT	20	20	50.0	45.0	57.0	57.8	2.3	0.0	1.135982	76171	76290	233-233	1.000000	71.3
ycf2_p2	ycf2	2	CCCAGCATTCATCAAGTTGG	TTCCAAACCAACCCCAATGT	20	20	50.0	45.0	57.0	57.8	2.3	0.0	1.135982	76171	76290	234-234	1.000000	71.3
ycf2_p3	ycf2	3	CCCAGCATTCATCAAGTTGG	AACCCCAATGTTGAGTAGCC	20	20	50.0	50.0	57.0	57.8	2.3	0.0	1.169408	76171	76290	225-225	1.000000	71.0
ycf2_p4	ycf2	4	CCCAGCATTCATCAAGTTGG	CCAAACCAACCCCAATGTTG	20	20	50.0	50.0	57.0	57.7	2.3	0.0	1.206442	76171	76290	232-232	1.000000	70.7
ycf2_p5	ycf2	5	CCCAGCATTCATCAAGTTGG	GGATTTCCAAACCAACCCCA	20	20	50.0	50.0	57.0	58.3	2.3	0.0	1.230178	76171	76290	238-238	1.000000	70.5
rps19-rpl22_p1	rps19-rpl22	1	TTCCAGGGACATGCGAAAAA	AACCACGAAGTTGATGAGCT	20	20	45.0	45.0	58.0	57.4	0.0	0.0	0.562537	83739	83829	259-260	1.000000	76.1
rps19-rpl22_p2	rps19-rpl22	2	TTTCCAGGGACATGCGAAAA	AACCACGAAGTTGATGAGCT	20	20	45.0	45.0	58.0	57.4	0.0	0.0	0.562537	83739	83829	260-261	1.000000	76.1
rps19-rpl22_p3	rps19-rpl22	3	TTTTCCAGGGACATGCGAAA	AACCACGAAGTTGATGAGCT	20	20	45.0	45.0	58.0	57.4	0.0	0.0	0.562537	83739	83829	261-262	1.000000	76.1
rps19-rpl22_p4	rps19-rpl22	4	TCCATTTTCCAGGGACATGC	AACCACGAAGTTGATGAGCT	20	20	50.0	45.0	58.1	57.4	0.0	0.0	0.699526	83739	83829	265-266	1.000000	75.0
rps19-rpl22_p5	rps19-rpl22	5	ATTTTCCAGGGACATGCGAA	AACCACGAAGTTGATGAGCT	20	20	45.0	45.0	57.8	57.4	0.0	0.0	0.765206	83739	83829	262-263	1.000000	74.5
rps3_p1	rps3	1	ACATTGGAGGATGTAACTGACTC	GCATAGCACTGCTCCAAGAT	23	20	43.5	50.0	57.9	58.0	0.0	6.9	3.126709	84289	84981	899-899	1.000000	55.2
rps3_p2	rps3	2	ACATTGGAGGATGTAACTGACTC	CTCAACGAGTCGCACACTAA	23	20	43.5	50.0	57.9	58.0	0.0	13.6	3.137544	84289	84981	919-919	1.000000	55.1
rps3_p3	rps3	3	ACATTGGAGGATGTAACTGACTC	TCAACGAGTCGCACACTAAG	23	20	43.5	50.0	57.9	58.0	0.0	13.6	3.137544	84289	84981	918-918	1.000000	55.1
rps3_p4	rps3	4	TGGAGGATGTAACTGACTCT	GCATAGCACTGCTCCAAGAT	20	20	45.0	50.0	54.9	58.0	0.0	6.9	3.161508	84289	84981	895-895	1.000000	54.9
rps3_p5	rps3	5	ACATTGGAGGATGTAACTGACTC	AGTCGCACACTAAGCATAGC	23	20	43.5	50.0	57.9	58.1	0.0	0.0	3.163366	84289	84981	912-912	1.000000	54.9
cemA_p1	cemA	1	TTCTCAGTCGGAACAAACCA	AGAGGGGAGGTAAAATGGGG	20	20	45.0	55.0	57.0	58.4	0.0	0.0	1.383700	97344	98129	902-902	1.000000	69.0
cemA_p2	cemA	2	TTCTCAGTCGGAACAAACCA	GGGAGGTAAAATGGGGGAGA	20	20	45.0	55.0	57.0	58.4	0.0	0.0	1.388491	97344	98129	898-898	1.000000	69.0
cemA_p3	cemA	3	TCTCAGTCGGAACAAACCATC	AGAGGGGAGGTAAAATGGGG	21	20	47.6	55.0	58.0	58.4	0.0	0.0	1.431723	97344	98129	901-901	1.000000	68.6
cemA_p4	cemA	4	TCTCAGTCGGAACAAACCATC	GGGAGGTAAAATGGGGGAGA	21	20	47.6	55.0	58.0	58.4	0.0	0.0	1.436513	97344	98129	897-897	1.000000	68.6
cemA_p5	cemA	5	CTCAGTCGGAACAAACCATCT	AGAGGGGAGGTAAAATGGGG	21	20	47.6	55.0	58.0	58.4	0.0	0.0	1.437176	97344	98129	900-900	1.000000	68.6
ycf1-rps12_p1	ycf1-rps12	1	ATACATCTGAGCTGGACCGA	TGGCACATACTCCTTTACGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.091794	108457	109232	990-990	1.000000	79.3
ycf1-rps12_p2	ycf1-rps12	2	TACATCTGAGCTGGACCGAT	TGGCACATACTCCTTTACGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.091794	108457	109232	989-989	1.000000	79.3
ycf1-rps12_p3	ycf1-rps12	3	AAACAGCACAATCGGAATGC	TGGCACATACTCCTTTACGC	20	20	45.0	50.0	57.6	58.0	4.6	0.0	0.371045	108457	109232	936-936	1.000000	77.1
ycf1-rps12_p4	ycf1-rps12	4	ATACATCTGAGCTGGACCGA	GGCACATACTCCTTTACGCT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.384376	108457	109232	989-989	1.000000	77.0
ycf1-rps12_p5	ycf1-rps12	5	TACATCTGAGCTGGACCGAT	GGCACATACTCCTTTACGCT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.384376	108457	109232	988-988	1.000000	77.0
psbJ-clpP_p1	psbJ-clpP	1	CGGAAGGATCCCTCTTTGGT	CGTGATTTTGCTCGTGATCG	20	20	55.0	50.0	59.1	57.9	8.6	0.0	1.150843	114706	115512	958-997	1.000000	74.1
psbJ-clpP_p2	psbJ-clpP	2	GGAAGGATCCCTCTTTGGTT	CGTGATTTTGCTCGTGATCG	20	20	50.0	50.0	56.8	57.9	8.6	0.0	1.268464	114706	115512	957-996	1.000000	73.1
psbJ-clpP_p3	psbJ-clpP	3	CGGAAGGATCCCTCTTTGGT	CCTACGCGATCAAGTGCTTA	20	20	55.0	50.0	59.1	57.8	8.6	15.9	1.291314	114706	115512	913-952	1.000000	73.1
psbJ-clpP_p4	psbJ-clpP	4	GGAAGGATCCCTCTTTGGTTG	CGTGATTTTGCTCGTGATCG	21	20	52.4	50.0	58.3	57.9	8.6	0.0	1.335323	114706	115512	957-996	1.000000	72.6
psbJ-clpP_p5	psbJ-clpP	5	GGAAGGATCCCTCTTTGGTT	CCTACGCGATCAAGTGCTTA	20	20	50.0	50.0	56.8	57.8	8.6	15.9	1.408934	114706	115512	912-951	1.000000	72.2
clpP-accD_p1	clpP-accD	1	CCTTGATAGGGCACTTTCGG	CATGGTTCCACGAGCTCTTT	20	20	55.0	50.0	58.3	58.2	0.0	12.8	0.518067	116266	117487	1132-1330	1.000000	80.1
clpP-accD_p2	clpP-accD	2	AGTTTGCTTCCTCCTCTTCG	CATGGTTCCACGAGCTCTTT	20	20	50.0	50.0	57.5	58.2	0.0	12.8	0.650644	116266	117487	1155-1353	1.000000	79.1
clpP-accD_p3	clpP-accD	3	ATTCGACCCAGTTTGCTTCC	CATGGTTCCACGAGCTCTTT	20	20	50.0	50.0	58.5	58.2	0.0	12.8	0.651758	116266	117487	1164-1362	1.000000	79.0
clpP-accD_p4	clpP-accD	4	GTTTGCTTCCTCCTCTTCGG	CATGGTTCCACGAGCTCTTT	20	20	55.0	50.0	58.6	58.2	0.0	12.8	0.742685	116266	117487	1154-1352	1.000000	78.3
clpP-accD_p5	clpP-accD	5	CCTTGATAGGGCACTTTCGG	GATACCATGGTTCCACGAGC	20	20	55.0	55.0	58.3	58.4	0.0	25.4	0.748527	116266	117487	1137-1335	1.000000	78.3
trnV-UAC_p1	trnV-UAC	1	TCCGTCTTGACAGAAAGTGA	ATTCTCTTCTGTGTTCCGCA	20	20	45.0	45.0	56.4	57.2	1.6	0.0	2.399277	124616	125221	783-783	1.000000	60.8
trnV-UAC_p2	trnV-UAC	2	TCCGTCTTGACAGAAAGTGA	TCTCTTCTGTGTTCCGCATT	20	20	45.0	45.0	56.4	57.2	1.6	0.0	2.399277	124616	125221	781-781	1.000000	60.8
trnV-UAC_p3	trnV-UAC	3	TCCGTCTTGACAGAAAGTGA	TTCTCTTCTGTGTTCCGCAT	20	20	45.0	45.0	56.4	57.2	1.6	0.0	2.399277	124616	125221	782-782	1.000000	60.8
trnV-UAC_p4	trnV-UAC	4	TCCGTCTTGACAGAAAGTGA	TCTCTTCTGTGTTCCGCATTT	20	21	45.0	42.9	56.4	57.9	1.6	0.0	2.685792	124616	125221	781-781	1.000000	58.5
trnV-UAC_p5	trnV-UAC	5	TCCGTCTTGACAGAAAGTGA	TTCTCTTCTGTGTTCCGCATT	20	21	45.0	42.9	56.4	57.9	1.6	0.0	2.685792	124616	125221	782-782	1.000000	58.5
trnV-UAC-ndhC_p1	trnV-UAC-ndhC	1	TGGGTTCTTGGAACAGTTCG	TTGAGAATGCCAGAATCGGG	20	20	50.0	50.0	58.0	58.0	7.2	0.0	0.080892	125222	126568	1517-1526	1.000000	79.6
trnV-UAC-ndhC_p2	trnV-UAC-ndhC	2	TGCATGTTGGGTTCTTGGAA	TTGAGAATGCCAGAATCGGG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.121856	125222	126568	1524-1533	1.000000	79.3
trnV-UAC-ndhC_p3	trnV-UAC-ndhC	3	AGAAGGTCTACGGTTCGAGT	TTGAGAATGCCAGAATCGGG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.239969	125222	126568	1459-1468	1.000000	78.4
trnV-UAC-ndhC_p4	trnV-UAC-ndhC	4	GGGTTCTTGGAACAGTTCGA	TTGAGAATGCCAGAATCGGG	20	20	50.0	50.0	57.8	58.0	7.2	0.0	0.292530	125222	126568	1516-1525	1.000000	77.9
trnV-UAC-ndhC_p5	trnV-UAC-ndhC	5	ATGCATGTTGGGTTCTTGGA	TTGAGAATGCCAGAATCGGG	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.350182	125222	126568	1525-1534	1.000000	77.5
ndhK_p1	ndhK	1	GTCTTTCTCTATCCGTGGGC	TCCTACTCGTTACTTTTTACTAACG	20	25	55.0	36.0	57.8	56.7	0.0	4.0	6.544972	126901	127707	941-941	1.000000	40.1
ndhK_p2	ndhK	2	TTTCTCTATCCGTGGGCCAT	TCCTACTCGTTACTTTTTACTAACG	20	25	50.0	36.0	58.5	56.7	10.7	4.0	6.811230	126901	127707	938-938	1.000000	40.1
ndhK_p3	ndhK	3	GTCTTTCTCTATCCGTGGGC	CCTACTCGTTACTTTTTACTAACG	20	24	55.0	37.5	57.8	55.4	0.0	4.0	6.837334	126901	127707	940-940	1.000000	40.1
ndhK_p4	ndhK	4	GTCTTTCTCTATCCGTGGGC	TTCCTACTCGTTACTTTTTACTAACG	20	26	55.0	34.6	57.8	57.3	0.0	4.0	6.954690	126901	127707	942-942	1.000000	40.1
ndhK_p5	ndhK	5	TTTCTCTATCCGTGGGCCAT	CCTACTCGTTACTTTTTACTAACG	20	24	50.0	37.5	58.5	55.4	10.7	4.0	7.103592	126901	127707	937-937	1.000000	40.1