primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	AGCAAGCATATTTCAATAAGCA	ACTGCGTATTCCACTTCGAC	22	20	31.8	50.0	54.8	58.0	8.9	0.0	5.251599	1303	3704	2507-2507	1.000000	40.1
trnK-UUU_p2	trnK-UUU	2	AGCAAGCATATTTCAATAAGCA	GCGTATTCCACTTCGACTGT	22	20	31.8	50.0	54.8	58.0	8.9	0.0	5.251599	1303	3704	2504-2504	1.000000	40.1
trnK-UUU_p3	trnK-UUU	3	AGCAAGCATATTTCAATAAGCA	TGCGTATTCCACTTCGACTG	22	20	31.8	50.0	54.8	58.0	8.9	0.0	5.252970	1303	3704	2505-2505	1.000000	40.1
trnK-UUU_p4	trnK-UUU	4	AGCAAGCATATTTCAATAAGCA	TTCCTGCCGTTCAATCACAA	22	20	31.8	45.0	54.8	58.0	8.9	0.0	5.269504	1303	3704	2567-2567	1.000000	40.1
trnK-UUU_p5	trnK-UUU	5	AGCAAGCATATTTCAATAAGCA	ATTCCTGCCGTTCAATCACA	22	20	31.8	45.0	54.8	57.8	8.9	0.0	5.442596	1303	3704	2568-2568	1.000000	40.1
matK-trnK-UUU_p1	matK-trnK-UUU	1	TGCGTAAAGATCTTCCCGAA	ACTGCGTATTCCACTTCGAC	20	20	45.0	50.0	57.0	58.0	23.3	0.0	1.050404	3058	3667	772-772	1.000000	71.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	TGCGTAAAGATCTTCCCGAA	GCGTATTCCACTTCGACTGT	20	20	45.0	50.0	57.0	58.0	23.3	0.0	1.050404	3058	3667	769-769	1.000000	71.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	TTGCGTAAAGATCTTCCCGA	ACTGCGTATTCCACTTCGAC	20	20	45.0	50.0	57.0	58.0	23.3	0.0	1.050404	3058	3667	773-773	1.000000	71.8
matK-trnK-UUU_p4	matK-trnK-UUU	4	TTGCGTAAAGATCTTCCCGA	GCGTATTCCACTTCGACTGT	20	20	45.0	50.0	57.0	58.0	23.3	0.0	1.050404	3058	3667	770-770	1.000000	71.8
matK-trnK-UUU_p5	matK-trnK-UUU	5	TGCGTAAAGATCTTCCCGAA	TGCGTATTCCACTTCGACTG	20	20	45.0	50.0	57.0	58.0	23.3	0.0	1.051774	3058	3667	770-770	1.000000	71.8
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCACTTAAAAGCCGAG	CGATGTGGTAGAAAGCAACG	20	20	50.0	50.0	58.6	57.2	6.4	0.0	1.358260	3705	5284	1655-1655	1.000000	69.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TAGTCGCACTTAAAAGCCGA	CGATGTGGTAGAAAGCAACG	20	20	45.0	50.0	57.3	57.2	3.0	0.0	1.516412	3705	5284	1656-1656	1.000000	67.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCGCACTTAAAAGCCGAGTA	CGATGTGGTAGAAAGCAACG	20	20	45.0	50.0	57.3	57.2	3.0	0.0	1.516412	3705	5284	1653-1653	1.000000	67.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CGCACTTAAAAGCCGAGTAC	CGATGTGGTAGAAAGCAACG	20	20	50.0	50.0	57.2	57.2	0.0	0.0	1.569921	3705	5284	1652-1652	1.000000	67.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGTCGCACTTAAAAGCCGAG	TGTGGTAGAAAGCAACGTGC	20	20	50.0	50.0	58.6	59.1	6.4	0.0	1.628995	3705	5284	1652-1652	1.000000	67.0
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AACTGGGGTTCGAGGATTTG	AGCCTTGATCGATAAAACGACA	20	22	50.0	40.9	57.8	58.1	0.0	0.0	2.343321	24479	25253	899-919	1.000000	63.0
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AACTGGGGTTCGAGGATTTG	GCCTTGATCGATAAAACGACA	20	21	50.0	42.9	57.8	56.7	0.0	0.0	2.480249	24479	25253	898-918	1.000000	61.9
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	TGCCTTACCACTTGGCTATG	AGCCTTGATCGATAAAACGACA	20	22	50.0	40.9	57.6	58.1	0.0	0.0	2.561316	24479	25253	871-891	1.000000	61.3
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	GAACTGGGGTTCGAGGATTT	AGCCTTGATCGATAAAACGACA	20	22	50.0	40.9	57.5	58.1	0.0	0.0	2.634623	24479	25253	900-920	1.000000	60.7
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	TGCCTTACCACTTGGCTATG	GCCTTGATCGATAAAACGACA	20	21	50.0	42.9	57.6	56.7	0.0	0.0	2.698244	24479	25253	870-890	1.000000	60.2
rpoC1_p1	rpoC1	1	TGCTCAGTCGAGAACTACGA	GGAAGGCTCCCTTGATCTCT	20	20	50.0	55.0	58.2	58.5	0.0	0.4	0.689374	28574	31393	2986-2996	1.000000	74.8
rpoC1_p2	rpoC1	2	TGCTCAGTCGAGAACTACGA	CTGGAAGGCTCCCTTGATCT	20	20	50.0	55.0	58.2	58.8	0.0	0.4	0.983943	28574	31393	2988-2998	1.000000	72.4
rpoC1_p3	rpoC1	3	TGCTCAGTCGAGAACTACGA	TGGAAGGCTCCCTTGATCTC	20	20	50.0	55.0	58.2	58.8	0.0	0.4	0.987608	28574	31393	2987-2997	1.000000	72.4
rpoC1_p4	rpoC1	4	TTGCTCAGTCGAGAACTACG	GGAAGGCTCCCTTGATCTCT	20	20	50.0	55.0	57.4	58.5	0.0	0.4	1.107705	28574	31393	2987-2997	1.000000	71.4
rpoC1_p5	rpoC1	5	TTGCTCAGTCGAGAACTACG	CTGGAAGGCTCCCTTGATCT	20	20	50.0	55.0	57.4	58.8	0.0	0.4	1.402274	28574	31393	2989-2999	1.000000	69.0
atpA_p1	atpA	1	CGATCGAGCATAATATAAACCTGC	AGCTCTTGTATCCTGTTCCA	24	20	41.7	45.0	58.0	55.9	10.3	0.0	6.174773	38975	40489	1689-1695	1.000000	40.1
atpA_p2	atpA	2	CGATCGAGCATAATATAAACCTGC	AGCTCTTGTATCCTGTTCCAA	24	21	41.7	42.9	58.0	56.6	10.3	0.0	6.384495	38975	40489	1689-1695	1.000000	40.1
atpA_p3	atpA	3	CGATCGAGCATAATATAAACCTGC	AGCTCTTGTATCCTGTTCCAAA	24	22	41.7	40.9	58.0	57.4	10.3	0.0	6.666479	38975	40489	1689-1695	1.000000	40.1
atpA_p4	atpA	4	CGATCGAGCATAATATAAACCTGC	CCATTGTGCTTGTAAATGTTGC	24	22	41.7	40.9	58.0	57.3	10.3	0.0	6.676252	38975	40489	1724-1730	1.000000	40.1
atpA_p5	atpA	5	CGATCGAGCATAATATAAACCTGC	GCTCTTGTATCCTGTTCCAAAC	24	22	41.7	45.5	58.0	57.3	10.3	0.0	6.712546	38975	40489	1688-1694	1.000000	40.1
trnF-GAA-ndhD_p1	trnF-GAA-ndhD	1	GCTAGTATGATGCACAGGGG	AGGTATTGGTCTTTACCCCGA	20	21	55.0	47.6	57.8	58.2	0.0	0.0	1.339211	44961	45235	460-460	1.000000	69.5
trnF-GAA-ndhD_p2	trnF-GAA-ndhD	2	GCTAGTATGATGCACAGGGG	GGTATTGGTCTTTACCCCGA	20	20	55.0	50.0	57.8	56.6	0.0	0.0	1.554543	44961	45235	459-459	1.000000	67.7
trnF-GAA-ndhD_p3	trnF-GAA-ndhD	3	GCTAGTATGATGCACAGGGG	AGGTATTGGTCTTTACCCCG	20	20	55.0	50.0	57.8	56.6	0.0	0.0	1.563878	44961	45235	460-460	1.000000	67.7
trnF-GAA-ndhD_p4	trnF-GAA-ndhD	4	GCTAGTATGATGCACAGGGG	TGGTCTTTACCCCGATCTAGT	20	21	55.0	47.6	57.8	57.6	0.0	0.0	1.569303	44961	45235	454-454	1.000000	67.6
trnF-GAA-ndhD_p5	trnF-GAA-ndhD	5	GTATGATGCACAGGGGAACA	AGGTATTGGTCTTTACCCCGA	20	21	50.0	47.6	57.6	58.2	0.0	0.0	1.581701	44961	45235	456-456	1.000000	67.5
rrn5-rrn4.5_p1	rrn5-rrn4.5	1	GATTGGTGTGGTTCCCCTAC	GCCAGCGATTAGACGATAGG	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.140305	58485	58786	423-423	1.000000	79.1
rrn5-rrn4.5_p2	rrn5-rrn4.5	2	AAGTTCGGGATGGATTGGTG	GCCAGCGATTAGACGATAGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144798	58485	58786	435-435	1.000000	79.0
rrn5-rrn4.5_p3	rrn5-rrn4.5	3	ACCAAGTTCGGGATGGATTG	GCCAGCGATTAGACGATAGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144798	58485	58786	438-438	1.000000	79.0
rrn5-rrn4.5_p4	rrn5-rrn4.5	4	CAAGTTCGGGATGGATTGGT	GCCAGCGATTAGACGATAGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144798	58485	58786	436-436	1.000000	79.0
rrn5-rrn4.5_p5	rrn5-rrn4.5	5	CACCAAGTTCGGGATGGATT	GCCAGCGATTAGACGATAGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144798	58485	58786	439-439	1.000000	79.0
trnI-GAU-rrn16_p1	trnI-GAU-rrn16	1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.2	57.7	0.0	7.8	0.485056	63812	64877	1028-1160	1.000000	80.2
trnI-GAU-rrn16_p2	trnI-GAU-rrn16	2	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.496177	63812	64877	1050-1182	1.000000	80.1
trnI-GAU-rrn16_p3	trnI-GAU-rrn16	3	GGCACAACGACGCAATTATC	AAGTCGTAACAAGGTAGCCG	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.548742	63812	64877	1037-1169	1.000000	79.7
trnI-GAU-rrn16_p4	trnI-GAU-rrn16	4	GGCACAACGACGCAATTATC	AACAAGGTAGCCGTACTGGA	20	20	50.0	50.0	58.2	58.4	0.0	7.8	0.553655	63812	64877	1030-1162	1.000000	79.6
trnI-GAU-rrn16_p5	trnI-GAU-rrn16	5	GGCACAACGACGCAATTATC	ACAAGGTAGCCGTACTGGAA	20	20	50.0	50.0	58.2	58.4	0.0	7.8	0.553655	63812	64877	1029-1161	1.000000	79.6
rpl16_p1	rpl16	1	TAACCATCTTGGAGCAGTGC	AGAATTGTTACCTCGGCAGA	20	20	50.0	45.0	57.9	56.6	7.1	0.0	1.557231	84473	85835	1466-1466	1.000000	67.6
rpl16_p2	rpl16	2	TAACCATCTTGGAGCAGTGC	AAGAATTGTTACCTCGGCAGA	20	21	50.0	42.9	57.9	57.3	7.1	0.0	1.809690	84473	85835	1467-1467	1.000000	65.6
rpl16_p3	rpl16	3	TAACCATCTTGGAGCAGTGC	AGAATTGTTACCTCGGCAGAA	20	21	50.0	42.9	57.9	57.3	7.1	0.0	1.809690	84473	85835	1466-1466	1.000000	65.6
rpl16_p4	rpl16	4	TAACCATCTTGGAGCAGTGC	AAGAATTGTTACCTCGGCAGAA	20	22	50.0	40.9	57.9	58.0	7.1	0.0	2.129732	84473	85835	1467-1467	1.000000	63.0
rpl16_p5	rpl16	5	TAACCATCTTGGAGCAGTGC	AGAATTGTTACCTCGGCAGAAA	20	22	50.0	40.9	57.9	58.0	7.1	0.0	2.129732	84473	85835	1466-1466	1.000000	63.0
petD-petB_p1	petD-petB	1	GATCTGCACAGCCTAACCAA	TGCACTTTCTAATGATCCGCA	20	21	50.0	42.9	57.9	58.0	0.0	0.0	1.129299	90510	90732	298-313	1.000000	74.9
petD-petB_p2	petD-petB	2	TTGATCTGCACAGCCTAACC	TGCACTTTCTAATGATCCGCA	20	21	50.0	42.9	57.9	58.0	0.0	0.0	1.129299	90510	90732	300-315	1.000000	74.9
petD-petB_p3	petD-petB	3	GATCTGCACAGCCTAACCAA	CCGCAAACAAGGTATTTCAGG	20	21	50.0	47.6	57.9	57.8	0.0	0.0	1.330008	90510	90732	282-297	1.000000	73.4
petD-petB_p4	petD-petB	4	TTGATCTGCACAGCCTAACC	CCGCAAACAAGGTATTTCAGG	20	21	50.0	47.6	57.9	57.8	0.0	0.0	1.330008	90510	90732	284-299	1.000000	73.4
petD-petB_p5	petD-petB	5	GTTCAAGTCACACACTCCCA	TGCACTTTCTAATGATCCGCA	20	21	50.0	42.9	57.7	58.0	0.0	0.0	1.341021	90510	90732	276-291	1.000000	73.3
ccsA-clpP1_p1	ccsA-clpP1	1	GGGTTACACAGCTATGGGTG	AAAGATGATTTGTTTTCTGTGT	20	22	55.0	27.3	58.0	52.8	6.7	0.0	7.276287	115575	116104	584-584	1.000000	40.0
ccsA-clpP1_p2	ccsA-clpP1	2	GGTTACACAGCTATGGGTGG	AAAGATGATTTGTTTTCTGTGT	20	22	55.0	27.3	58.0	52.8	13.1	0.0	7.276287	115575	116104	583-583	1.000000	40.0
ccsA-clpP1_p3	ccsA-clpP1	3	GAAAGGGGATAAACCGGCAA	AAAGATGATTTGTTTTCTGTGT	20	22	50.0	27.3	58.2	52.8	0.0	0.0	7.395848	115575	116104	669-669	1.000000	40.0
ccsA-clpP1_p4	ccsA-clpP1	4	GGGTTACACAGCTATGGGTG	AAAAGATGATTTGTTTTCTGTGT	20	23	55.0	26.1	58.0	53.6	6.7	0.0	7.427532	115575	116104	585-585	1.000000	40.0
ccsA-clpP1_p5	ccsA-clpP1	5	GGTTACACAGCTATGGGTGG	AAAAGATGATTTGTTTTCTGTGT	20	23	55.0	26.1	58.0	53.6	13.1	0.0	7.427532	115575	116104	584-584	1.000000	40.0
clpP1_p1	clpP1	1	TATAGCATTCCTCGCGTTGG	GCTTATCAAAACCCGGTCCA	20	20	50.0	50.0	57.9	58.2	5.8	0.0	0.319936	116105	116782	816-816	1.000000	77.6
clpP1_p2	clpP1	2	TATAGCATTCCTCGCGTTGG	TGCTTATCAAAACCCGGTCC	20	20	50.0	50.0	57.9	58.2	5.8	0.0	0.319936	116105	116782	817-817	1.000000	77.6
clpP1_p3	clpP1	3	TATAGCATTCCTCGCGTTGG	TCAAAACCCGGTCCAAGAAA	20	20	50.0	45.0	57.9	57.6	5.8	0.0	0.587716	116105	116782	811-811	1.000000	75.5
clpP1_p4	clpP1	4	TATAGCATTCCTCGCGTTGG	ATCAAAACCCGGTCCAAGAA	20	20	50.0	45.0	57.9	57.3	5.8	0.0	0.817059	116105	116782	812-812	1.000000	73.7
clpP1_p5	clpP1	5	CACGAGGTGCTCCATTCTTA	GCTTATCAAAACCCGGTCCA	20	20	50.0	50.0	57.3	58.2	7.7	0.0	0.843982	116105	116782	791-791	1.000000	73.5
clpP1-accD_p1	clpP1-accD	1	CTCTGCATCGAAGGCTGATT	TTGTCGCACTCGGAGTTATG	20	20	50.0	50.0	58.0	58.0	17.8	8.2	0.058373	116783	117224	1310-1311	1.000000	79.6
clpP1-accD_p2	clpP1-accD	2	TCTGCATCGAAGGCTGATTC	TTGTCGCACTCGGAGTTATG	20	20	50.0	50.0	58.1	58.0	14.9	8.2	0.063813	116783	117224	1309-1310	1.000000	79.6
clpP1-accD_p3	clpP1-accD	3	ACCTTAGGGATGGGGTTTGA	TTGTCGCACTCGGAGTTATG	20	20	50.0	50.0	57.9	58.0	2.3	8.2	0.078032	116783	117224	898-899	1.000000	79.5
clpP1-accD_p4	clpP1-accD	4	ACTCCCTCTGTCATTCCTCC	TTGTCGCACTCGGAGTTATG	20	20	55.0	50.0	58.1	58.0	0.0	8.2	0.136428	116783	117224	1105-1106	1.000000	79.0
clpP1-accD_p5	clpP1-accD	5	CTCCCTCTGTCATTCCTCCA	TTGTCGCACTCGGAGTTATG	20	20	55.0	50.0	58.1	58.0	0.0	8.2	0.137783	116783	117224	1104-1105	1.000000	79.0
accD_p1	accD	1	AAAGAGCTCGTGGAACCATG	ACCGAATGAATAGGATGAAAA	20	21	50.0	33.3	58.2	52.9	8.4	0.0	6.284673	117225	119444	2363-2458	1.000000	40.0
accD_p2	accD	2	AAAGAGCTCGTGGAACCATG	ACCGAATGAATAGGATGAAAAA	20	22	50.0	31.8	58.2	53.8	8.4	0.0	6.399413	117225	119444	2363-2458	1.000000	40.0
accD_p3	accD	3	AAAAGAGCTCGTGGAACCAT	ACCGAATGAATAGGATGAAAA	20	21	45.0	33.3	57.4	52.9	8.4	0.0	6.666809	117225	119444	2364-2459	1.000000	40.0
accD_p4	accD	4	AAAGAGCTCGTGGAACCATG	TGGATTTTTACTGCTTAAAGTTGT	20	24	50.0	29.2	58.2	55.5	8.4	0.0	6.722842	117225	119444	2304-2399	1.000000	40.0
accD_p5	accD	5	AAAAGAGCTCGTGGAACCAT	ACCGAATGAATAGGATGAAAAA	20	22	45.0	31.8	57.4	53.8	8.4	0.0	6.781549	117225	119444	2364-2459	1.000000	40.0
ndhJ-chlN_p1	ndhJ-chlN	1	GTTGGATTGGGTGGCCTTTA	ATTCATGGGTTTGCCAATGC	20	20	50.0	45.0	58.1	57.6	3.3	0.0	0.474961	127142	128066	1106-1107	1.000000	76.3
ndhJ-chlN_p2	ndhJ-chlN	2	TTGGATTGGGTGGCCTTTAC	ATTCATGGGTTTGCCAATGC	20	20	50.0	45.0	58.1	57.6	3.3	0.0	0.474961	127142	128066	1105-1106	1.000000	76.3
ndhJ-chlN_p3	ndhJ-chlN	3	GTTGGATTGGGTGGCCTTTA	CCAGATTCATGGGTTTGCCA	20	20	50.0	50.0	58.1	58.4	3.3	0.0	0.508997	127142	128066	1110-1111	1.000000	76.0
ndhJ-chlN_p4	ndhJ-chlN	4	TTGGATTGGGTGGCCTTTAC	CCAGATTCATGGGTTTGCCA	20	20	50.0	50.0	58.1	58.4	3.3	0.0	0.508997	127142	128066	1109-1110	1.000000	76.0
ndhJ-chlN_p5	ndhJ-chlN	5	GCCTGAAAGTTGGATTGGGT	ATTCATGGGTTTGCCAATGC	20	20	50.0	45.0	58.4	57.6	0.0	0.0	0.774007	127142	128066	1114-1115	1.000000	73.9