primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpl32-ycf1_p1	rpl32-ycf1	1	AGTAAGGGGGCAATACCTGT	GCCTACTCAATACTAGGCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.086154	110994	111172	1054-1054	0.500000	59.3
rpl32-ycf1_p2	rpl32-ycf1	2	GGGATATTGGGCAGCGTTAA	GCCTACTCAATACTAGGCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.091754	110994	111172	544-544	0.500000	59.3
rpl32-ycf1_p3	rpl32-ycf1	3	AGTAAGGGGGCAATACCTGT	AAAACCAGTTGATGCGGCTA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.114454	110994	111172	987-987	0.500000	59.1
rpl32-ycf1_p4	rpl32-ycf1	4	AGTAAGGGGGCAATACCTGT	TAAAACCAGTTGATGCGGCT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.114454	110994	111172	988-988	0.500000	59.1
rpl32-ycf1_p5	rpl32-ycf1	5	GGGATATTGGGCAGCGTTAA	AAAACCAGTTGATGCGGCTA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.120053	110994	111172	477-477	0.500000	59.0
ycf1_p1	ycf1	1	ACGAACAATAGAAGTCGGCC	GCCATAGGTTTTCCCCGATT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.073336	111173	113360	2996-2996	0.500000	59.4
ycf1_p2	ycf1	2	TAGAAGTCGGCCTAACCCAA	GCCATAGGTTTTCCCCGATT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.125988	111173	113360	2988-2988	0.500000	59.0
ycf1_p3	ycf1	3	GAAGTCGGCCTAACCCAAAA	GCCATAGGTTTTCCCCGATT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.167120	111173	113360	2986-2986	0.500000	58.7
ycf1_p4	ycf1	4	ATAGAAGTCGGCCTAACCCA	GCCATAGGTTTTCCCCGATT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.237378	111173	113360	2989-2989	0.500000	58.1
ycf1_p5	ycf1	5	ACGAACAATAGAAGTCGGCC	ATTGGACGCTAAGACAAGGC	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.266904	111173	113360	2407-2407	0.500000	57.9
ycf1-rps15_p1	ycf1-rps15	1	ACGGGCTCATAGGAGTAGAC	GCCATAGGTTTTCCCCGATT	20	20	55.0	50.0	58.0	57.9	6.2	0.0	0.083300	113361	113630	1062-1062	0.500000	59.3
ycf1-rps15_p2	ycf1-rps15	2	CACGGGCTCATAGGAGTAGA	GCCATAGGTTTTCCCCGATT	20	20	55.0	50.0	58.0	57.9	6.2	0.0	0.084705	113361	113630	1063-1063	0.500000	59.3
ycf1-rps15_p3	ycf1-rps15	3	CGGGCTCATAGGAGTAGACA	GCCATAGGTTTTCCCCGATT	20	20	55.0	50.0	58.0	57.9	6.2	0.0	0.084705	113361	113630	1061-1061	0.500000	59.3
ycf1-rps15_p4	ycf1-rps15	4	TCACGGGCTCATAGGAGTAG	GCCATAGGTTTTCCCCGATT	20	20	55.0	50.0	58.0	57.9	6.2	0.0	0.084705	113361	113630	1064-1064	0.500000	59.3
ycf1-rps15_p5	ycf1-rps15	5	ACCTAGACGTCGTCGAGAAT	GCCATAGGTTTTCCCCGATT	20	20	50.0	50.0	58.0	57.9	29.8	0.0	0.088721	113361	113630	966-966	0.500000	59.3
rps15_p1	rps15	1	ACGGGCTCATAGGAGTAGAC	GCCATAGGTTTTCCCCGATT	20	20	55.0	50.0	58.0	57.9	6.2	0.0	0.083300	113631	113897	1062-1062	0.500000	59.3
rps15_p2	rps15	2	CACGGGCTCATAGGAGTAGA	GCCATAGGTTTTCCCCGATT	20	20	55.0	50.0	58.0	57.9	6.2	0.0	0.084705	113631	113897	1063-1063	0.500000	59.3
rps15_p3	rps15	3	CGGGCTCATAGGAGTAGACA	GCCATAGGTTTTCCCCGATT	20	20	55.0	50.0	58.0	57.9	6.2	0.0	0.084705	113631	113897	1061-1061	0.500000	59.3
rps15_p4	rps15	4	TCACGGGCTCATAGGAGTAG	GCCATAGGTTTTCCCCGATT	20	20	55.0	50.0	58.0	57.9	6.2	0.0	0.084705	113631	113897	1064-1064	0.500000	59.3
rps15_p5	rps15	5	ACCTAGACGTCGTCGAGAAT	GCCATAGGTTTTCCCCGATT	20	20	50.0	50.0	58.0	57.9	29.8	0.0	0.088721	113631	113897	966-966	0.500000	59.3
ndhH_p1	ndhH	1	GCCTTGTCTTAGCGTCCAAT	CGTTGTTGTATACCTGCGGA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.261885	114018	115199	1969-1969	0.500000	57.9
ndhH_p2	ndhH	2	GCCTTGTCTTAGCGTCCAAT	TGACTGCTATAGAGGGTCCG	20	20	50.0	55.0	58.3	58.0	0.0	3.2	0.278273	114018	115199	2106-2106	0.500000	57.8
ndhH_p3	ndhH	3	GCCTTGTCTTAGCGTCCAAT	TGAAGAATTCCCAAAGGGCC	20	20	50.0	50.0	58.3	58.1	0.0	0.6	0.323742	114018	115199	2008-2008	0.500000	57.4
ndhH_p4	ndhH	4	GCCTTGTCTTAGCGTCCAAT	CCGTCTGCTAGAGCTTGAAG	20	20	50.0	55.0	58.3	58.1	0.0	2.7	0.341015	114018	115199	2023-2023	0.500000	57.3
ndhH_p5	ndhH	5	GGGGGTCCGTTGAATTTCAA	CGTTGTTGTATACCTGCGGA	20	20	50.0	50.0	58.4	58.0	7.7	0.0	0.377788	114018	115199	1697-1697	0.500000	57.0
ndhA_p1	ndhA	1	GGTTTATCGGGACCAATGCT	CGTACCGCTTGTTGACCATA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.053734	115201	117387	2901-2901	0.500000	59.6
ndhA_p2	ndhA	2	TGGGAAGTCCAATGGCAAAA	CGTACCGCTTGTTGACCATA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.095052	115201	117387	2814-2814	0.500000	59.2
ndhA_p3	ndhA	3	TTCGTTCCCCGGGTTTTATC	CGTACCGCTTGTTGACCATA	20	20	50.0	50.0	57.9	58.0	21.8	0.0	0.113332	115201	117387	2492-2492	0.500000	59.1
ndhA_p4	ndhA	4	AGTAAAAAGCCATCCCCTGC	CGTACCGCTTGTTGACCATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.148319	115201	117387	2619-2619	0.500000	58.8
ndhA_p5	ndhA	5	GTAAAAAGCCATCCCCTGCT	CGTACCGCTTGTTGACCATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.148319	115201	117387	2618-2618	0.500000	58.8
ndhA-ndhI_p1	ndhA-ndhI	1	TGGGTGGTTGGAATCTCTCT	CGTACCGCTTGTTGACCATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045744	117388	117519	448-448	0.500000	59.6
ndhA-ndhI_p2	ndhA-ndhI	2	TGGGTGGTTGGAATCTCTCT	AAAACGCTCCGATGTGATCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.138099	117388	117519	557-557	0.500000	58.9
ndhA-ndhI_p3	ndhA-ndhI	3	TGGGTGGTTGGAATCTCTCT	AAACGCTCCGATGTGATCAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.138099	117388	117519	556-556	0.500000	58.9
ndhA-ndhI_p4	ndhA-ndhI	4	TGGGTGGTTGGAATCTCTCT	GGTAAACGACCCAGAGCAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.154217	117388	117519	835-835	0.500000	58.8
ndhA-ndhI_p5	ndhA-ndhI	5	TGCGAGAGATTGCCTTTTGA	CGTACCGCTTGTTGACCATA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.184430	117388	117519	599-599	0.500000	58.5
ndhI_p1	ndhI	1	TGGGTGGTTGGAATCTCTCT	ACCAACGAAATAGCCCCATC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.097575	117520	118035	1589-1589	0.500000	59.2
ndhI_p2	ndhI	2	TGGGTGGTTGGAATCTCTCT	TAATACCACTCCGAGACCCC	20	20	50.0	55.0	58.0	57.9	0.0	1.3	0.122480	117520	118035	1365-1365	0.500000	59.0
ndhI_p3	ndhI	3	TGGGTGGTTGGAATCTCTCT	AAGACCTGACCCCAGAAAGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.163196	117520	118035	1338-1338	0.500000	58.7
ndhI_p4	ndhI	4	TGGGTGGTTGGAATCTCTCT	ATTCTGGCCCACTCATGAAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.179582	117520	118035	1537-1537	0.500000	58.6
ndhI_p5	ndhI	5	TGGGTGGTTGGAATCTCTCT	CTGGCCCACTCATGAACATT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.201424	117520	118035	1534-1534	0.500000	58.4
ndhI-ndhG_p1	ndhI-ndhG	1	TGATCACATCGGAGCGTTTT	ACCAACGAAATAGCCCCATC	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.146089	118036	118402	1052-1052	0.500000	58.8
ndhI-ndhG_p2	ndhI-ndhG	2	TTGATCACATCGGAGCGTTT	ACCAACGAAATAGCCCCATC	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.146089	118036	118402	1053-1053	0.500000	58.8
ndhI-ndhG_p3	ndhI-ndhG	3	TGATCACATCGGAGCGTTTT	TAATACCACTCCGAGACCCC	20	20	45.0	55.0	57.9	57.9	0.0	1.3	0.170994	118036	118402	828-828	0.500000	58.6
ndhI-ndhG_p4	ndhI-ndhG	4	TTGATCACATCGGAGCGTTT	TAATACCACTCCGAGACCCC	20	20	45.0	55.0	57.9	57.9	0.0	1.3	0.170994	118036	118402	829-829	0.500000	58.6
ndhI-ndhG_p5	ndhI-ndhG	5	TGATCACATCGGAGCGTTTT	AAGACCTGACCCCAGAAAGA	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.211710	118036	118402	801-801	0.500000	58.3
ndhG_p1	ndhG	1	TGATCACATCGGAGCGTTTT	AGTCCAATAGCAGCTTCAGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.128836	118403	118933	1760-1760	0.500000	59.0
ndhG_p2	ndhG	2	TTGATCACATCGGAGCGTTT	AGTCCAATAGCAGCTTCAGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.128836	118403	118933	1761-1761	0.500000	59.0
ndhG_p3	ndhG	3	AAAAGAATGCGATGGGTCCA	AGTCCAATAGCAGCTTCAGC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.249906	118403	118933	1171-1171	0.500000	58.0
ndhG_p4	ndhG	4	AAAGAATGCGATGGGTCCAA	AGTCCAATAGCAGCTTCAGC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.249906	118403	118933	1170-1170	0.500000	58.0
ndhG_p5	ndhG	5	TCAAATTGCTCTGGGTCGTT	AGTCCAATAGCAGCTTCAGC	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.305882	118403	118933	1486-1486	0.500000	57.6
ndhG-ndhE_p1	ndhG-ndhE	1	GATGGGGCTATTTCGTTGGT	GCCTTACATCCATCCCAAGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.065900	118934	119185	1234-1234	0.500000	59.5
ndhG-ndhE_p2	ndhG-ndhE	2	GATGGGGCTATTTCGTTGGT	AGTCCAATAGCAGCTTCAGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.088312	118934	119185	728-728	0.500000	59.3
ndhG-ndhE_p3	ndhG-ndhE	3	GGGGTCTCGGAGTGGTATTA	GCCTTACATCCATCCCAAGG	20	20	55.0	55.0	57.9	58.0	4.7	0.0	0.090805	118934	119185	1458-1458	0.500000	59.3
ndhG-ndhE_p4	ndhG-ndhE	4	GGGGTCTCGGAGTGGTATTA	AGTCCAATAGCAGCTTCAGC	20	20	55.0	50.0	57.9	58.0	4.7	0.0	0.113217	118934	119185	952-952	0.500000	59.1
ndhG-ndhE_p5	ndhG-ndhE	5	TCTTTCTGGGGTCAGGTCTT	GCCTTACATCCATCCCAAGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.131521	118934	119185	1485-1485	0.500000	58.9
ndhE_p1	ndhE	1	GATGGGGCTATTTCGTTGGT	GCCTTACATCCATCCCAAGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.065900	119186	119491	1234-1234	0.500000	59.5
ndhE_p2	ndhE	2	GATGGGGCTATTTCGTTGGT	AACCTGCAAATAGGGGCAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.075500	119186	119491	1550-1550	0.500000	59.4
ndhE_p3	ndhE	3	GATGGGGCTATTTCGTTGGT	ACCTGCAAATAGGGGCAAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.075500	119186	119491	1549-1549	0.500000	59.4
ndhE_p4	ndhE	4	GGGGTCTCGGAGTGGTATTA	GCCTTACATCCATCCCAAGG	20	20	55.0	55.0	57.9	58.0	4.7	0.0	0.090805	119186	119491	1458-1458	0.500000	59.3
ndhE_p5	ndhE	5	GGGGTCTCGGAGTGGTATTA	ACCTGCAAATAGGGGCAAAA	20	20	55.0	45.0	57.9	58.0	4.7	0.0	0.100405	119186	119491	1773-1773	0.500000	59.2
ndhE-psaC_p1	ndhE-psaC	1	GCTGAAGCTGCTATTGGACT	TTTGGCCACTGTACATTGCT	20	20	50.0	45.0	58.0	58.0	0.9	3.9	0.041574	119492	119817	1163-1163	0.500000	59.7
ndhE-psaC_p2	ndhE-psaC	2	GCTGAAGCTGCTATTGGACT	GCCTTACATCCATCCCAAGG	20	20	50.0	55.0	58.0	58.0	0.9	0.0	0.048648	119492	119817	526-526	0.500000	59.6
ndhE-psaC_p3	ndhE-psaC	3	GCTGAAGCTGCTATTGGACT	AGAATTTCGAGTAACCGGCC	20	20	50.0	50.0	58.0	58.0	0.9	0.0	0.055478	119492	119817	1120-1120	0.500000	59.6
ndhE-psaC_p4	ndhE-psaC	4	GCTGAAGCTGCTATTGGACT	AACCTGCAAATAGGGGCAAA	20	20	50.0	45.0	58.0	58.0	0.9	0.0	0.058248	119492	119817	842-842	0.500000	59.5
ndhE-psaC_p5	ndhE-psaC	5	GCTGAAGCTGCTATTGGACT	ACCTGCAAATAGGGGCAAAA	20	20	50.0	45.0	58.0	58.0	0.9	0.0	0.058248	119492	119817	841-841	0.500000	59.5
ndhD_p1	ndhD	1	CCTTGGGATGGATGTAAGGC	GGACAAGGCCTAAGCAAGTT	20	20	55.0	50.0	58.0	58.1	0.0	5.2	0.102060	120186	121688	1971-1971	0.500000	59.2
ndhD_p2	ndhD	2	CCTTGGGATGGATGTAAGGC	AAAGCACTTCTCCCCTTTCG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.115558	120186	121688	2374-2374	0.500000	59.1
ndhD_p3	ndhD	3	CCTTGGGATGGATGTAAGGC	ATTCGGTCTGGCCTTTCTTC	20	20	55.0	50.0	58.0	57.9	0.0	3.3	0.128460	120186	121688	1882-1882	0.500000	59.0
ndhD_p4	ndhD	4	TACGAGCTTGTCCTACGGAT	GGACAAGGCCTAAGCAAGTT	20	20	50.0	50.0	58.0	58.1	10.7	5.2	0.137387	120186	121688	2006-2006	0.500000	58.9
ndhD_p5	ndhD	5	TACGAGCTTGTCCTACGGAT	AAAGCACTTCTCCCCTTTCG	20	20	50.0	50.0	58.0	58.1	10.7	0.0	0.150884	120186	121688	2409-2409	0.500000	58.8
ndhD-ccsA_p1	ndhD-ccsA	1	GGCATGAGTGGTTTCATTGC	ATGCAGCTCTTTTATGCGGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043115	121689	121907	1097-1097	0.500000	59.7
ndhD-ccsA_p2	ndhD-ccsA	2	GGCATGAGTGGTTTCATTGC	TGCAGCTCTTTTATGCGGAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043115	121689	121907	1096-1096	0.500000	59.7
ndhD-ccsA_p3	ndhD-ccsA	3	GTTTACCCGGATTTCGCTCT	ATGCAGCTCTTTTATGCGGA	20	20	50.0	45.0	58.0	58.0	0.7	0.0	0.062634	121689	121907	836-836	0.500000	59.5
ndhD-ccsA_p4	ndhD-ccsA	4	GTTTACCCGGATTTCGCTCT	TGCAGCTCTTTTATGCGGAT	20	20	50.0	45.0	58.0	58.0	0.7	0.0	0.062634	121689	121907	835-835	0.500000	59.5
ndhD-ccsA_p5	ndhD-ccsA	5	GTATTGGCGTTTACCCGGAT	TGCAGCTCTTTTATGCGGAT	20	20	50.0	45.0	58.0	58.0	0.7	0.0	0.085018	121689	121907	843-843	0.500000	59.3
ccsA_p1	ccsA	1	GGCATGAGTGGTTTCATTGC	GGGATATTGGGCAGCGTTAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024770	121908	122888	1931-1931	0.500000	59.8
ccsA_p2	ccsA	2	GTTTACCCGGATTTCGCTCT	GGGATATTGGGCAGCGTTAA	20	20	50.0	50.0	58.0	58.0	0.7	0.0	0.044288	121908	122888	1670-1670	0.500000	59.6
ccsA_p3	ccsA	3	GGCATGAGTGGTTTCATTGC	AACTCCTCCAATCTGGACGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.061845	121908	122888	1786-1786	0.500000	59.5
ccsA_p4	ccsA	4	GTATTGGCGTTTACCCGGAT	GGGATATTGGGCAGCGTTAA	20	20	50.0	50.0	58.0	58.0	0.7	0.0	0.066672	121908	122888	1678-1678	0.500000	59.5
ccsA_p5	ccsA	5	GGCATGAGTGGTTTCATTGC	CTTAGGAAGCAGTGCGAGAG	20	20	50.0	55.0	58.0	58.1	0.0	10.0	0.080511	121908	122888	1700-1700	0.500000	59.4
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	ATCCGCATAAAAGAGCTGCA	GGGATATTGGGCAGCGTTAA	20	20	45.0	50.0	58.0	58.0	1.0	0.0	0.067026	123085	123193	855-855	0.500000	59.5
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	TCCGCATAAAAGAGCTGCAT	GGGATATTGGGCAGCGTTAA	20	20	45.0	50.0	58.0	58.0	1.0	0.0	0.067026	123085	123193	854-854	0.500000	59.5
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	CTCGCACTGCTTCCTAAGAG	GGGATATTGGGCAGCGTTAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.104422	123085	123193	249-249	0.500000	59.2
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CTCTCGCACTGCTTCCTAAG	GGGATATTGGGCAGCGTTAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.104422	123085	123193	251-251	0.500000	59.2
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	TCCGTTAAGAGTCCCGAAGT	GGGATATTGGGCAGCGTTAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.109199	123085	123193	754-754	0.500000	59.1