primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
tatC_p1	tatC	1	TAAACTTGACCACGACCACG	ACCACAAAGTACAGAAACTGCT	20	22	50.0	40.9	58.1	58.1	0.0	0.0	2.258364	13578	14324	1680-1680	0.333333	35.3
tatC_p2	tatC	2	TAAACTTGACCACGACCACG	CCACAAAGTACAGAAACTGCTG	20	22	50.0	45.5	58.1	57.8	0.0	0.0	2.359467	13578	14324	1679-1679	0.333333	34.5
tatC_p3	tatC	3	TAAACTTGACCACGACCACG	ACCACAAAGTACAGAAACTGC	20	21	50.0	42.9	58.1	56.7	0.0	0.0	2.475192	13578	14324	1680-1680	0.333333	33.5
tatC_p4	tatC	4	AACAGATGTTGGGGCTTCAA	ACCACAAAGTACAGAAACTGCT	20	22	45.0	40.9	57.6	58.1	0.0	0.0	2.493941	13578	14324	1318-1318	0.333333	33.4
tatC_p5	tatC	5	ACAGATGTTGGGGCTTCAAA	ACCACAAAGTACAGAAACTGCT	20	22	45.0	40.9	57.6	58.1	0.0	0.0	2.493941	13578	14324	1317-1317	0.333333	33.4
trnH(GUG)-rpl19_p1	trnH(GUG)-rpl19	1	GCACTTGGAATTGTTGGAGC	AACCCAGTCGGATGCATTTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.146906	19034	20348	2224-2224	0.333333	52.2
trnH(GUG)-rpl19_p2	trnH(GUG)-rpl19	2	GCACTTGGAATTGTTGGAGC	ACCCAGTCGGATGCATTTTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.146906	19034	20348	2223-2223	0.333333	52.2
trnH(GUG)-rpl19_p3	trnH(GUG)-rpl19	3	TGGCACTTGGAATTGTTGGA	AACCCAGTCGGATGCATTTT	20	20	45.0	45.0	57.9	58.1	0.0	0.0	0.155814	19034	20348	2226-2226	0.333333	52.1
trnH(GUG)-rpl19_p4	trnH(GUG)-rpl19	4	TGGCACTTGGAATTGTTGGA	ACCCAGTCGGATGCATTTTT	20	20	45.0	45.0	57.9	58.1	0.0	0.0	0.155814	19034	20348	2225-2225	0.333333	52.1
trnH(GUG)-rpl19_p5	trnH(GUG)-rpl19	5	TAAGATTCAGGCGAACGACG	ACCCAGTCGGATGCATTTTT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.172500	19034	20348	1787-1787	0.333333	52.0
rps2_p1	rps2	1	GACTAATCCCAGCAGGTTCG	CGTTTGTTGGAGCAGCAAAT	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.197271	33720	34472	1415-1415	0.333333	51.8
rps2_p2	rps2	2	GACTAATCCCAGCAGGTTCG	GGAATTAAAGCTCCTGCCCA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.202551	33720	34472	1365-1365	0.333333	51.7
rps2_p3	rps2	3	GACTAATCCCAGCAGGTTCG	AGCTCCTGCCCAATTACAAC	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.219251	33720	34472	1357-1357	0.333333	51.6
rps2_p4	rps2	4	GACTAATCCCAGCAGGTTCG	GTCCCATTTGAGTGATGGCA	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.220563	33720	34472	1280-1280	0.333333	51.6
rps2_p5	rps2	5	GACTAATCCCAGCAGGTTCG	TATCGTTTGTTGGAGCAGCA	20	20	55.0	45.0	58.1	57.8	0.0	0.0	0.236069	33720	34472	1418-1418	0.333333	51.4
psbW-clpC_p1	psbW-clpC	1	AATTGGGGTGTAGCCAAGTG	CGCCAATTGTAAAGCACCAG	20	20	50.0	50.0	58.1	58.0	2.2	4.4	0.095391	45227	45861	1789-1789	0.333333	52.6
psbW-clpC_p2	psbW-clpC	2	TAGGTTCGAATCCTCCCTCC	CGCCAATTGTAAAGCACCAG	20	20	55.0	50.0	57.9	58.0	18.6	4.4	0.102375	45227	45861	1738-1738	0.333333	52.5
psbW-clpC_p3	psbW-clpC	3	AATTGGGGTGTAGCCAAGTG	GAACCAGCAACAATTCGAGC	20	20	50.0	50.0	58.1	58.0	2.2	0.0	0.107541	45227	45861	1422-1422	0.333333	52.5
psbW-clpC_p4	psbW-clpC	4	TAGGTTCGAATCCTCCCTCC	GAACCAGCAACAATTCGAGC	20	20	55.0	50.0	57.9	58.0	18.6	0.0	0.114525	45227	45861	1371-1371	0.333333	52.4
psbW-clpC_p5	psbW-clpC	5	CCCTCCTCGCCCATTTATTC	CGCCAATTGTAAAGCACCAG	20	20	55.0	50.0	58.1	58.0	0.0	4.4	0.121186	45227	45861	1844-1844	0.333333	52.4
dnaB_p1	dnaB	1	CCTCTTGAGATTAAGACAGGCC	CCTGCGTTCTACCAACTGAA	22	20	50.0	50.0	58.2	57.8	0.0	0.0	2.360951	50414	52186	2183-2183	0.333333	34.4
dnaB_p2	dnaB	2	CCTCTTGAGATTAAGACAGGCC	ACCAACTGAACTAAGGACGC	22	20	50.0	50.0	58.2	57.8	0.0	0.0	2.362452	50414	52186	2173-2173	0.333333	34.4
dnaB_p3	dnaB	3	CCTCTTGAGATTAAGACAGGCC	AAAAGAACGGATGCAGGAGG	22	20	50.0	50.0	58.2	58.2	0.0	0.0	2.370749	50414	52186	2518-2518	0.333333	34.4
dnaB_p4	dnaB	4	CCTCTTGAGATTAAGACAGGCC	ACCCTCGACACCAGATTTTG	22	20	50.0	50.0	58.2	57.8	0.0	0.0	2.382138	50414	52186	2207-2207	0.333333	34.3
dnaB_p5	dnaB	5	CCTCTTGAGATTAAGACAGGCC	CAGATTTTGGAGACCTGCGT	22	20	50.0	50.0	58.2	58.2	0.0	0.0	2.383620	50414	52186	2196-2196	0.333333	34.3
acpP-trnP_p1	acpP-trnP	1	GGAACAACTGTACGCGTAGT	AAAGCAGACACGCTACCAAA	20	20	50.0	45.0	57.9	58.0	21.3	0.0	0.094301	54539	55740	494-1479	0.666667	77.9
acpP-trnP_p2	acpP-trnP	2	TGTAGGAACAACTGTACGCG	AAAGCAGACACGCTACCAAA	20	20	50.0	45.0	57.9	58.0	3.5	0.0	0.092894	54539	55740	1483-1483	0.333333	52.6
acpP-trnP_p3	acpP-trnP	3	GTAGGAACAACTGTACGCGT	AAAGCAGACACGCTACCAAA	20	20	50.0	45.0	57.9	58.0	19.2	0.0	0.094301	54539	55740	1482-1482	0.333333	52.6
acpP-trnP_p4	acpP-trnP	4	TGTAGGAACAACTGTACGCG	GGGATGACAGGATTCGAACC	20	20	50.0	55.0	57.9	58.0	3.5	0.0	0.099760	54539	55740	1520-1520	0.333333	52.5
acpP-trnP_p5	acpP-trnP	5	GGAACAACTGTACGCGTAGT	GGGATGACAGGATTCGAACC	20	20	50.0	55.0	57.9	58.0	21.3	0.0	0.101167	54539	55740	1516-1516	0.333333	52.5
rpl4_p1	rpl4	1	ACGACGTTTATGACCACCAC	TCGTGGAGCAATGACTCATG	20	20	50.0	50.0	58.2	58.0	0.0	6.9	0.227323	82666	83310	1394-1394	0.333333	51.5
rpl4_p2	rpl4	2	ACGACGTTTATGACCACCAC	GTCGTGGAGCAATGACTCAT	20	20	50.0	50.0	58.2	58.0	0.0	6.9	0.228723	82666	83310	1395-1395	0.333333	51.5
rpl4_p3	rpl4	3	ACGACGTTTATGACCACCAC	GTGGAGCAATGACTCATGGT	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.332789	82666	83310	1392-1392	0.333333	50.7
rpl4_p4	rpl4	4	ACGACGTTTATGACCACCAC	GGAACTACTCCAGGACGAGT	20	20	50.0	55.0	58.2	58.2	0.0	0.0	0.376185	82666	83310	1336-1336	0.333333	50.3
rpl4_p5	rpl4	5	GACGTTTATGACCACCACCT	TCGTGGAGCAATGACTCATG	20	20	50.0	50.0	57.5	58.0	0.0	6.9	0.481901	82666	83310	1392-1392	0.333333	49.5
psbD-rns_p1	psbD-rns	1	TTCAACCGTTGCTCCATGAA	ATTCTCACGCGTTACTCACC	20	20	45.0	50.0	58.0	58.0	6.4	10.2	0.033565	91844	93923	2853-2853	0.333333	53.1
psbD-rns_p2	psbD-rns	2	TTTCAACCGTTGCTCCATGA	ATTCTCACGCGTTACTCACC	20	20	45.0	50.0	58.0	58.0	6.4	10.2	0.033565	91844	93923	2854-2854	0.333333	53.1
psbD-rns_p3	psbD-rns	3	AAAGCTAGGGCATTGTACGG	ATTCTCACGCGTTACTCACC	20	20	50.0	50.0	58.0	58.0	0.0	10.2	0.046217	91844	93923	2633-2633	0.333333	53.0
psbD-rns_p4	psbD-rns	4	TTGGCTGCATCTCCATCTTC	ATTCTCACGCGTTACTCACC	20	20	50.0	50.0	58.0	58.0	0.5	10.2	0.056274	91844	93923	2885-2885	0.333333	52.9
psbD-rns_p5	psbD-rns	5	GCTGCATCTCCATCTTCGAA	ATTCTCACGCGTTACTCACC	20	20	50.0	50.0	58.1	58.0	0.0	10.2	0.062443	91844	93923	2882-2882	0.333333	52.8
rnl-psaC_p1	rnl-psaC	1	AGTTATCGTGCCAACGGTAC	GCCTGGGGTTCTTATTGGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.059022	98617	99456	1549-1549	0.333333	52.9
rnl-psaC_p2	rnl-psaC	2	TGATCTGACGATGCTGTGTG	GCCTGGGGTTCTTATTGGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.059022	98617	99456	1853-1853	0.333333	52.9
rnl-psaC_p3	rnl-psaC	3	GTGATCTGACGATGCTGTGT	GCCTGGGGTTCTTATTGGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.060405	98617	99456	1854-1854	0.333333	52.9
rnl-psaC_p4	rnl-psaC	4	TCTTTCGGGACTGAAGGAGT	GCCTGGGGTTCTTATTGGAG	20	20	50.0	55.0	58.0	57.9	2.0	0.0	0.069715	98617	99456	2102-2102	0.333333	52.8
rnl-psaC_p5	rnl-psaC	5	AGTTATCGTGCCAACGGTAC	AAGGCTGGCAAGGAGAAAAA	20	20	50.0	45.0	58.0	57.9	0.0	3.4	0.087040	98617	99456	1455-1455	0.333333	52.6
ccs1_p1	ccs1	1	ACCTCTTGGTTCCTTGGTTC	TAAATGGACGACGCCGAAAA	20	20	50.0	45.0	57.3	58.2	0.0	0.0	0.865549	102735	104105	2044-2044	0.333333	46.4
ccs1_p2	ccs1	2	ACCTCTTGGTTCCTTGGTTC	TTAAATGGACGACGCCGAAA	20	20	50.0	45.0	57.3	58.2	0.0	0.0	0.865549	102735	104105	2045-2045	0.333333	46.4
ccs1_p3	ccs1	3	ACCTCTTGGTTCCTTGGTTC	TTTAAATGGACGACGCCGAA	20	20	50.0	45.0	57.3	58.2	0.0	0.0	0.865549	102735	104105	2046-2046	0.333333	46.4
ccs1_p4	ccs1	4	CCTCTTGGTTCCTTGGTTCT	TAAATGGACGACGCCGAAAA	20	20	50.0	45.0	57.0	58.2	0.0	0.0	1.166207	102735	104105	2043-2043	0.333333	44.0
ccs1_p5	ccs1	5	CCTCTTGGTTCCTTGGTTCT	TTAAATGGACGACGCCGAAA	20	20	50.0	45.0	57.0	58.2	0.0	0.0	1.166207	102735	104105	2044-2044	0.333333	44.0
secA-rpl27_p1	secA-rpl27	1	TAATGGCGTTCTTGCTTCGT	TCGTGGACATAGACAACCCT	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.263644	106980	107911	1932-1932	0.333333	51.2
secA-rpl27_p2	secA-rpl27	2	TAATGGCGTTCTTGCTTCGT	CGATTGCACAGACTTCAGGA	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.281275	106980	107911	2185-2185	0.333333	51.1
secA-rpl27_p3	secA-rpl27	3	AAACCGGCTAAGATTTGGCT	TCGTGGACATAGACAACCCT	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.282921	106980	107911	1538-1538	0.333333	51.1
secA-rpl27_p4	secA-rpl27	4	AAACCGGCTAAGATTTGGCT	CGATTGCACAGACTTCAGGA	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.300552	106980	107911	1791-1791	0.333333	50.9
secA-rpl27_p5	secA-rpl27	5	TAATGGCGTTCTTGCTTCGT	GGGCAGGAAGTACACGAAAT	20	20	45.0	50.0	58.2	57.9	0.0	0.0	0.306091	106980	107911	1807-1807	0.333333	50.9
ycf54_p1	ycf54	1	TCCTGAAGTCTGTGCAATCG	TCCTGAAGTCTGTGCAATCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.169219	109044	109787	1913-1913	0.333333	52.0
ycf54_p2	ycf54	2	CCCCACATTCTAGCCAAACA	TCCTGAAGTCTGTGCAATCG	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.298986	109044	109787	1785-1785	0.333333	50.9
ycf54_p3	ycf54	3	TCCTGAAGTCTGTGCAATCG	CCCCACATTCTAGCCAAACA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.298986	109044	109787	1785-1785	0.333333	50.9
ycf54_p4	ycf54	4	CCCCACATTCTAGCCAAACA	CCCCACATTCTAGCCAAACA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.428752	109044	109787	1657-1657	0.333333	49.9
ycf54_p5	ycf54	5	TCCTGAAGTCTGTGCAATCG	ACCATTGACCCGGTTTTAGC	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.543230	109044	109787	1944-1944	0.333333	49.0
rpl27-secA_p1	rpl27-secA	1	TCGTGGACATAGACAACCCT	TAATGGCGTTCTTGCTTCGT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.263644	111025	111956	1932-1932	0.333333	51.2
rpl27-secA_p2	rpl27-secA	2	CGATTGCACAGACTTCAGGA	TAATGGCGTTCTTGCTTCGT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.281275	111025	111956	2185-2185	0.333333	51.1
rpl27-secA_p3	rpl27-secA	3	TCGTGGACATAGACAACCCT	AAACCGGCTAAGATTTGGCT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.282921	111025	111956	1538-1538	0.333333	51.1
rpl27-secA_p4	rpl27-secA	4	CGATTGCACAGACTTCAGGA	AAACCGGCTAAGATTTGGCT	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.300552	111025	111956	1791-1791	0.333333	50.9
rpl27-secA_p5	rpl27-secA	5	GGGCAGGAAGTACACGAAAT	TAATGGCGTTCTTGCTTCGT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.306091	111025	111956	1807-1807	0.333333	50.9
ccs1_p1	ccs1	1	TAAATGGACGACGCCGAAAA	ACCTCTTGGTTCCTTGGTTC	20	20	45.0	50.0	58.2	57.3	0.0	0.0	0.865549	114831	116201	2044-2044	0.333333	46.4
ccs1_p2	ccs1	2	TTAAATGGACGACGCCGAAA	ACCTCTTGGTTCCTTGGTTC	20	20	45.0	50.0	58.2	57.3	0.0	0.0	0.865549	114831	116201	2045-2045	0.333333	46.4
ccs1_p3	ccs1	3	TTTAAATGGACGACGCCGAA	ACCTCTTGGTTCCTTGGTTC	20	20	45.0	50.0	58.2	57.3	0.0	0.0	0.865549	114831	116201	2046-2046	0.333333	46.4
ccs1_p4	ccs1	4	TAAATGGACGACGCCGAAAA	CCTCTTGGTTCCTTGGTTCT	20	20	45.0	50.0	58.2	57.0	0.0	0.0	1.166207	114831	116201	2043-2043	0.333333	44.0
ccs1_p5	ccs1	5	TTAAATGGACGACGCCGAAA	CCTCTTGGTTCCTTGGTTCT	20	20	45.0	50.0	58.2	57.0	0.0	0.0	1.166207	114831	116201	2044-2044	0.333333	44.0
ccs1-trnN_p1	ccs1-trnN	1	GGTTGGTTTACTTCGATTCCA	AACATCGTAGCTGCTCATGG	21	20	42.9	50.0	56.2	58.1	0.0	4.9	2.882211	116202	116807	1913-1913	0.333333	30.3
ccs1-trnN_p2	ccs1-trnN	2	GGTTGGTTTACTTCGATTCCA	CATCGTAGCTGCTCATGGTT	21	20	42.9	50.0	56.2	58.1	0.0	4.9	2.882211	116202	116807	1911-1911	0.333333	30.3
ccs1-trnN_p3	ccs1-trnN	3	GGTTGGTTTACTTCGATTCCA	TTCTTCTTAGCAGCTTGGCC	21	20	42.9	50.0	56.2	58.2	0.0	0.0	3.001213	116202	116807	1835-1835	0.333333	29.3
ccs1-trnN_p4	ccs1-trnN	4	GGTTGGTTTACTTCGATTCCA	ATCCGTGAAACAGCTGAAGG	21	20	42.9	50.0	56.2	58.2	0.0	23.3	3.014085	116202	116807	1982-1982	0.333333	29.2
ccs1-trnN_p5	ccs1-trnN	5	TGTTGGGTAGGAGGATTAACA	CATCGTAGCTGCTCATGGTT	21	20	42.9	50.0	56.0	58.1	0.0	4.9	3.087022	116202	116807	1278-1278	0.333333	28.6
psaC-rnl_p1	psaC-rnl	1	GCCTGGGGTTCTTATTGGAG	AGTTATCGTGCCAACGGTAC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.059022	119480	120319	1549-1549	0.333333	52.9
psaC-rnl_p2	psaC-rnl	2	GCCTGGGGTTCTTATTGGAG	TGATCTGACGATGCTGTGTG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.059022	119480	120319	1853-1853	0.333333	52.9
psaC-rnl_p3	psaC-rnl	3	GCCTGGGGTTCTTATTGGAG	GTGATCTGACGATGCTGTGT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.060405	119480	120319	1854-1854	0.333333	52.9
psaC-rnl_p4	psaC-rnl	4	GCCTGGGGTTCTTATTGGAG	TCTTTCGGGACTGAAGGAGT	20	20	55.0	50.0	57.9	58.0	0.0	2.0	0.069715	119480	120319	2102-2102	0.333333	52.8
psaC-rnl_p5	psaC-rnl	5	AAGGCTGGCAAGGAGAAAAA	AGTTATCGTGCCAACGGTAC	20	20	45.0	50.0	57.9	58.0	3.4	0.0	0.087040	119480	120319	1455-1455	0.333333	52.6
rns-psbD_p1	rns-psbD	1	ATTCTCACGCGTTACTCACC	TTCAACCGTTGCTCCATGAA	20	20	50.0	45.0	58.0	58.0	10.2	6.4	0.033565	125013	127092	2853-2853	0.333333	53.1
rns-psbD_p2	rns-psbD	2	ATTCTCACGCGTTACTCACC	TTTCAACCGTTGCTCCATGA	20	20	50.0	45.0	58.0	58.0	10.2	6.4	0.033565	125013	127092	2854-2854	0.333333	53.1
rns-psbD_p3	rns-psbD	3	ATTCTCACGCGTTACTCACC	AAAGCTAGGGCATTGTACGG	20	20	50.0	50.0	58.0	58.0	10.2	0.0	0.046217	125013	127092	2633-2633	0.333333	53.0
rns-psbD_p4	rns-psbD	4	ATTCTCACGCGTTACTCACC	TTGGCTGCATCTCCATCTTC	20	20	50.0	50.0	58.0	58.0	10.2	0.5	0.056274	125013	127092	2885-2885	0.333333	52.9
rns-psbD_p5	rns-psbD	5	ATTCTCACGCGTTACTCACC	GCTGCATCTCCATCTTCGAA	20	20	50.0	50.0	58.0	58.1	10.2	0.0	0.062443	125013	127092	2882-2882	0.333333	52.8