primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf21_p1	ycf21	1	ACCCAATGACATGAATGGTA	CGTCAGATAAGAATTGAATTTTCT	20	24	40.0	29.2	54.3	53.7	0.0	0.0	11.977104	54326	54850	740-740	0.500000	20.0
ycf21_p2	ycf21	2	TACCCAATGACATGAATGGT	CGTCAGATAAGAATTGAATTTTCT	20	24	40.0	29.2	54.3	53.7	0.0	0.0	11.977104	54326	54850	741-741	0.500000	20.0
ycf21_p3	ycf21	3	ACCCAATGACATGAATGGTA	CGTCAGATAAGAATTGAATTTTCTTCT	20	27	40.0	29.6	54.3	56.7	0.0	6.8	11.984748	54326	54850	740-740	0.500000	20.0
ycf21_p4	ycf21	4	TACCCAATGACATGAATGGT	CGTCAGATAAGAATTGAATTTTCTTCT	20	27	40.0	29.6	54.3	56.7	0.0	6.8	11.984748	54326	54850	741-741	0.500000	20.0
ycf21_p5	ycf21	5	ACCCAATGACATGAATGGTA	CGTCAGATAAGAATTGAATTTTCTTC	20	26	40.0	30.8	54.3	55.5	0.0	6.8	12.180541	54326	54850	740-740	0.500000	20.0
ycf60_p1	ycf60	1	GCAGCTCCTAAACGAATTGC	TGCCACTGATTGAGCTCTTC	20	20	50.0	50.0	57.8	57.9	0.0	16.2	0.284955	66642	67259	1589-1589	0.500000	57.7
ycf60_p2	ycf60	2	GCAGCTCCTAAACGAATTGC	GCAGCGCAACCAATATCTTC	20	20	50.0	50.0	57.8	57.9	0.0	11.5	0.290990	66642	67259	1906-1906	0.500000	57.7
ycf60_p3	ycf60	3	GCAGCTCCTAAACGAATTGC	TAACAGCAGCGCAACCAATA	20	20	50.0	45.0	57.8	58.2	0.0	11.5	0.365156	66642	67259	1911-1911	0.500000	57.1
ycf60_p4	ycf60	4	GCAGCTCCTAAACGAATTGC	AGCAGCGCAACCAATATCTT	20	20	50.0	45.0	57.8	58.2	0.0	11.5	0.424769	66642	67259	1907-1907	0.500000	56.6
ycf60_p5	ycf60	5	GCAGCTCCTAAACGAATTGC	TCAGCATTTTTGCATCCAGC	20	20	50.0	45.0	57.8	57.6	0.0	0.3	0.553489	66642	67259	1675-1675	0.500000	55.6
ccdA_p1	ccdA	1	AAATTTCCACTGACGTGCAC	AGGATAGGCGAGGGTACTTC	20	20	45.0	55.0	57.2	58.0	6.8	0.0	0.776216	70154	70885	1669-1941	1.000000	82.2
ccdA_p2	ccdA	2	AAATTTCCACTGACGTGCAC	GGTTCAAATCCTTCCTGGGG	20	20	45.0	55.0	57.2	58.1	6.8	0.0	0.921806	70154	70885	1150-1153	1.000000	81.0
ccdA_p3	ccdA	3	AAATTTCCACTGACGTGCAC	TACCAATTTCACCACGTCCG	20	20	45.0	50.0	57.2	58.2	6.8	0.0	0.976155	70154	70885	1486-1758	1.000000	80.6
ccdA_p4	ccdA	4	AAATTTCCACTGACGTGCAC	ACGCCAAACCATCCAATGTA	20	20	45.0	45.0	57.2	57.8	6.8	0.0	0.982571	70154	70885	1788-2062	1.000000	80.5
ccdA_p5	ccdA	5	AAATTTCCACTGACGTGCAC	TACGCCAAACCATCCAATGT	20	20	45.0	45.0	57.2	57.8	6.8	0.0	0.982571	70154	70885	1789-2063	1.000000	80.5
ycf56_p1	ycf56	1	ATAGTTTCTCCTGGCTGTGC	CAGCTGGTTCATGGGCTTTA	20	20	50.0	50.0	57.6	58.2	0.0	5.6	0.571836	74882	75571	1315-1315	0.500000	55.4
ycf56_p2	ycf56	2	ATAGTTTCTCCTGGCTGTGC	GTTTTGGCAGCTGGTTCATG	20	20	50.0	50.0	57.6	58.2	0.0	17.1	0.622936	74882	75571	1322-1322	0.500000	55.0
ycf56_p3	ycf56	3	CCTGGCTGTGCATGTACTTT	CAGCTGGTTCATGGGCTTTA	20	20	50.0	50.0	58.5	58.2	15.2	5.6	0.623840	74882	75571	1306-1306	0.500000	55.0
ycf56_p4	ycf56	4	CCTGGCTGTGCATGTACTTT	GTTTTGGCAGCTGGTTCATG	20	20	50.0	50.0	58.5	58.2	15.2	17.1	0.674940	74882	75571	1313-1313	0.500000	54.6
ycf56_p5	ycf56	5	ACCAACATTCATGCCTGGAT	CAGCTGGTTCATGGGCTTTA	20	20	45.0	50.0	57.5	58.2	13.2	5.6	0.699521	74882	75571	1690-1690	0.500000	54.4
ycf37_p1	ycf37	1	TCGGGATATAGCGCAGTTTG	AATCCTCTTGAAGCTCGTGG	20	20	50.0	50.0	57.9	57.6	11.5	0.0	0.542187	86323	86826	1306-1466	1.000000	85.8
ycf37_p2	ycf37	2	GTCGGGATATAGCGCAGTTT	AATCCTCTTGAAGCTCGTGG	20	20	50.0	50.0	57.8	57.6	11.5	0.0	0.543669	86323	86826	1307-1307	0.500000	55.7
ycf37_p3	ycf37	3	TACTGTCGGTTGGGTACGTA	AATCCTCTTGAAGCTCGTGG	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.585935	86323	86826	1715-1715	0.500000	55.3
ycf37_p4	ycf37	4	TCGGGATATAGCGCAGTTTG	ATCCTCTTGAAGCTCGTGGA	20	20	50.0	50.0	57.9	58.4	11.5	0.0	0.587273	86323	86826	1305-1305	0.500000	55.3
ycf37_p5	ycf37	5	GTCGGGATATAGCGCAGTTT	ATCCTCTTGAAGCTCGTGGA	20	20	50.0	50.0	57.8	58.4	11.5	0.0	0.588754	86323	86826	1306-1306	0.500000	55.3
ycf37-chlN_p1	ycf37-chlN	1	TCGGGATATAGCGCAGTTTG	TGATGCGCAGGAGAAAAACT	20	20	50.0	45.0	57.9	58.1	11.5	18.3	0.252696	86827	87410	1746-1746	0.500000	58.0
ycf37-chlN_p2	ycf37-chlN	2	GTCGGGATATAGCGCAGTTT	TGATGCGCAGGAGAAAAACT	20	20	50.0	45.0	57.8	58.1	11.5	18.3	0.254178	86827	87410	1747-1747	0.500000	58.0
ycf37-chlN_p3	ycf37-chlN	3	TCGGGATATAGCGCAGTTTG	TACAACATTGATGCGCAGGA	20	20	50.0	45.0	57.9	57.9	11.5	18.3	0.255393	86827	87410	1754-1754	0.500000	58.0
ycf37-chlN_p4	ycf37-chlN	4	GTCGGGATATAGCGCAGTTT	TACAACATTGATGCGCAGGA	20	20	50.0	45.0	57.8	57.9	11.5	18.3	0.256874	86827	87410	1755-1755	0.500000	57.9
ycf37-chlN_p5	ycf37-chlN	5	TCGGGATATAGCGCAGTTTG	ACATTGATGCGCAGGAGAAA	20	20	50.0	45.0	57.9	58.2	11.5	18.3	0.323825	86827	87410	1750-1750	0.500000	57.4
upp_p1	upp	1	ACAGCCATGGCAAGAAAGAA	GCTCGTCCATCTCTTCGTTT	20	20	45.0	50.0	58.0	58.0	37.2	0.0	0.002683	98652	99251	1798-1798	0.500000	60.0
upp_p2	upp	2	ATGAGATCACCTAGTGGCGA	GCTCGTCCATCTCTTCGTTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.075583	98652	99251	1926-1926	0.500000	59.4
upp_p3	upp	3	TCCATTGGTTACGCAAGCTT	GCTCGTCCATCTCTTCGTTT	20	20	45.0	50.0	58.1	58.0	1.9	0.0	0.098044	98652	99251	1271-1271	0.500000	59.2
upp_p4	upp	4	CTGCTGGCTTTGAGAAAGGA	GCTCGTCCATCTCTTCGTTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.100740	98652	99251	1595-1595	0.500000	59.2
upp_p5	upp	5	ATCCATTGGTTACGCAAGCT	GCTCGTCCATCTCTTCGTTT	20	20	45.0	50.0	57.9	58.0	1.9	0.0	0.131101	98652	99251	1272-1272	0.500000	59.0
orf160_p1	orf160	1	AAACGAAGAGATGGACGAGC	TTGTTCCAGGTGGAGGATCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047124	100379	100876	1437-1437	0.500000	59.6
orf160_p2	orf160	2	AAACGAAGAGATGGACGAGC	GTTCCAGGTGGAGGATCTCT	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.129558	100379	100876	1435-1435	0.500000	59.0
orf160_p3	orf160	3	AAACGAAGAGATGGACGAGC	ATGTTGGGTACAGCAAGACG	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.193920	100379	100876	1720-1720	0.500000	58.4
orf160_p4	orf160	4	CGAAGAGATGGACGAGCAAT	TTGTTCCAGGTGGAGGATCT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.260700	100379	100876	1434-1434	0.500000	57.9
orf160_p5	orf160	5	AAACGAAGAGATGGACGAGC	TGATCCCGCGAAAGTAACTC	20	20	50.0	50.0	58.0	57.7	0.0	8.2	0.291292	100379	100876	1181-1181	0.500000	57.7
hisS_p1	hisS	1	TTTTAATGCGGCCTGTCGTA	ATCCATAAACTGGGGTGGGA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.101821	102781	104040	1938-1938	0.500000	59.2
hisS_p2	hisS	2	TTAATGCGGCCTGTCGTATC	ATCCATAAACTGGGGTGGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.144925	102781	104040	1936-1936	0.500000	58.8
hisS_p3	hisS	3	TTTTAATGCGGCCTGTCGTA	TAAACTGGGGTGGGAGGATT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.165051	102781	104040	1933-1933	0.500000	58.7
hisS_p4	hisS	4	TTTTAATGCGGCCTGTCGTA	CTTTGGGCTGACCGTTGATA	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.204170	102781	104040	2285-2285	0.500000	58.4
hisS_p5	hisS	5	TTTTAATGCGGCCTGTCGTA	CTTACCACTTGGCTACACCC	20	20	45.0	55.0	57.9	57.9	0.0	0.0	0.205645	102781	104040	1879-1879	0.500000	58.4
syfB_p1	syfB	1	AATCCTCCCACCCCAGTTTA	AGTAAGGGTGCAAAGGTGTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.087575	104709	106703	2750-2802	1.000000	87.7
syfB_p2	syfB	2	AATCCTCCCACCCCAGTTTA	GGTGCAAAGGTGTGGTAAGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.093172	104709	106703	2744-2796	1.000000	87.7
syfB_p3	syfB	3	GGGTGTAGCCAAGTGGTAAG	AGTAAGGGTGCAAAGGTGTG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.128168	104709	106703	2804-2856	1.000000	87.4
syfB_p4	syfB	4	GGTGTAGCCAAGTGGTAAGG	AGTAAGGGTGCAAAGGTGTG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.128168	104709	106703	2803-2855	1.000000	87.4
syfB_p5	syfB	5	TATCAACGGTCAGCCCAAAG	AGTAAGGGTGCAAAGGTGTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.126693	104709	106703	2398-2398	0.500000	59.0
ycf29_p1	ycf29	1	ATTATTCGCGGGTTCGAGTC	GTTTTTGTGATTGCGGTTGC	20	20	50.0	45.0	58.2	57.4	6.1	0.0	0.794778	108268	108918	966-966	0.500000	53.6
ycf29_p2	ycf29	2	ATTATTCGCGGGTTCGAGTC	TTGTAACATTCACGCGTTGG	20	20	50.0	45.0	58.2	57.3	6.1	10.2	0.823091	108268	108918	1226-1226	0.500000	53.4
ycf29_p3	ycf29	3	CAAGGGGTTAAGGCAATGGA	GTTTTTGTGATTGCGGTTGC	20	20	50.0	45.0	57.8	57.4	0.0	0.0	0.874004	108268	108918	996-996	0.500000	53.0
ycf29_p4	ycf29	4	CAAGGGGTTAAGGCAATGGA	TTGTAACATTCACGCGTTGG	20	20	50.0	45.0	57.8	57.3	0.0	10.2	0.902317	108268	108918	1256-1256	0.500000	52.8
ycf29_p5	ycf29	5	GCTAACCTCAACCTCCTCCT	GTTTTTGTGATTGCGGTTGC	20	20	55.0	45.0	58.4	57.4	0.0	0.0	1.069612	108268	108918	1318-1318	0.500000	51.4
rps5_p1	rps5	1	TGAAAATCCACCGCCAGAAA	GTTGGAGAAGACATTGGCCA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.102007	136505	137056	891-891	0.500000	59.2
rps5_p2	rps5	2	TTGAAAATCCACCGCCAGAA	GTTGGAGAAGACATTGGCCA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.102007	136505	137056	892-892	0.500000	59.2
rps5_p3	rps5	3	TGAAAATCCACCGCCAGAAA	AGTTGGAGAAGACATTGGCC	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.218283	136505	137056	892-892	0.500000	58.3
rps5_p4	rps5	4	TTGAAAATCCACCGCCAGAA	AGTTGGAGAAGACATTGGCC	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.218283	136505	137056	893-893	0.500000	58.3
rps5_p5	rps5	5	TGAAAATCCACCGCCAGAAA	GGCAAAGGTATACGCTACGA	20	20	45.0	50.0	58.0	57.5	0.0	0.0	0.508813	136505	137056	1140-1140	0.500000	55.9
accB-ycf45_p1	accB-ycf45	1	TCGCATTAGAGCTTGTCCAC	CTTGCATACGCCTTGCTCTA	20	20	50.0	50.0	58.0	58.1	0.0	6.4	0.070171	152898	153305	1396-1402	1.000000	88.8
accB-ycf45_p2	accB-ycf45	2	AATGACCAGGTTTTGCCCAT	CTTGCATACGCCTTGCTCTA	20	20	45.0	50.0	58.0	58.1	0.0	6.4	0.088587	152898	153305	1690-1690	0.500000	59.3
accB-ycf45_p3	accB-ycf45	3	ATGACCAGGTTTTGCCCATT	CTTGCATACGCCTTGCTCTA	20	20	45.0	50.0	58.0	58.1	0.0	6.4	0.088587	152898	153305	1689-1689	0.500000	59.3
accB-ycf45_p4	accB-ycf45	4	TCGCATTAGAGCTTGTCCAC	TGGAAAACGAGCTTCTGGTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.131192	152898	153305	996-996	0.500000	59.0
accB-ycf45_p5	accB-ycf45	5	ATGACCAGGTTTTGCCCATT	TGGAAAACGAGCTTCTGGTC	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.149608	152898	153305	1283-1283	0.500000	58.8
chlB_p1	chlB	1	TGCAGTTACAATGTTGGAGACA	TCTTGAGAACCCCTAGCCAA	22	20	40.9	50.0	58.2	58.0	0.0	0.0	2.224238	157665	157784	777-777	0.500000	42.2
chlB_p2	chlB	2	TGCAGTTACAATGTTGGAGACA	TTCTTGAGAACCCCTAGCCA	22	20	40.9	50.0	58.2	58.0	0.0	0.0	2.224238	157665	157784	778-778	0.500000	42.2
chlB_p3	chlB	3	TGCAGTTACAATGTTGGAGACA	TCGGCTTCAAAACCATACGT	22	20	40.9	45.0	58.2	57.8	0.0	0.0	2.358226	157665	157784	650-650	0.500000	41.1
chlB_p4	chlB	4	TGCAGTTACAATGTTGGAGACA	CGGCTTCAAAACCATACGTG	22	20	40.9	50.0	58.2	57.8	0.0	0.0	2.426634	157665	157784	649-649	0.500000	40.6
chlB_p5	chlB	5	TGCAGTTACAATGTTGGAGACA	AACCCCTAGCCAATACATGC	22	20	40.9	50.0	58.2	57.6	0.0	0.0	2.550347	157665	157784	770-770	0.500000	39.6
orf456_p1	orf456	1	GGCCCAGCTCATATTGGTAC	TCTTGAGAACCCCTAGCCAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.141303	156791	158155	2210-2210	0.500000	58.9
orf456_p2	orf456	2	GGCCCAGCTCATATTGGTAC	TTCTTGAGAACCCCTAGCCA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.141303	156791	158155	2211-2211	0.500000	58.9
orf456_p3	orf456	3	GGCCCAGCTCATATTGGTAC	AACCCTCCAACCTCACGATA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.155456	156791	158155	2681-2681	0.500000	58.8
orf456_p4	orf456	4	GGCCCAGCTCATATTGGTAC	ACCCTCCAACCTCACGATAA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.155456	156791	158155	2680-2680	0.500000	58.8
orf456_p5	orf456	5	ATGCACCACTAGGGGATGAT	TCTTGAGAACCCCTAGCCAA	20	20	50.0	50.0	58.2	58.0	20.8	0.0	0.213758	156791	158155	2146-2146	0.500000	58.3
acsF-petN_p1	acsF-petN	1	TGCAGTGCATGATCTTTCCA	AGACTGATTGCCCATTCCAC	20	20	45.0	50.0	57.8	57.9	15.4	0.0	0.338259	160811	161262	1139-1139	0.500000	57.3
acsF-petN_p2	acsF-petN	2	TGCAGTGCATGATCTTTCCA	AGAGCCCCATCCTAAACTGA	20	20	45.0	50.0	57.8	57.7	15.4	0.0	0.473904	160811	161262	746-746	0.500000	56.2
acsF-petN_p3	acsF-petN	3	AAGCCCGAAAATTCTGCAGT	AGACTGATTGCCCATTCCAC	20	20	45.0	50.0	58.4	57.9	14.7	0.0	0.513967	160811	161262	1153-1153	0.500000	55.9
acsF-petN_p4	acsF-petN	4	TGCAGTGCATGATCTTTCCA	AGCCCCATCCTAAACTGACA	20	20	45.0	50.0	57.8	58.3	15.4	0.0	0.534644	160811	161262	744-744	0.500000	55.7
acsF-petN_p5	acsF-petN	5	TGCAGTGCATGATCTTTCCA	GAGCCCCATCCTAAACTGAC	20	20	45.0	55.0	57.8	57.7	15.4	0.0	0.537159	160811	161262	745-745	0.500000	55.7