primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CTCTAGACCTAGCCGCTCTT	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.153260	69	407	405-405	1.000000	78.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CTCTAGACCTAGCCGCTCTT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.157622	69	407	419-419	1.000000	78.7
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CTCTAGACCTAGCCGCTCTT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.159129	69	407	418-418	1.000000	78.7
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CTCTAGACCTAGCCGCTCTT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.159129	69	407	420-420	1.000000	78.7
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TTTCCCTCTAGACCTAGCCG	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.197754	69	407	410-410	1.000000	78.4
psbA_p1	psbA	1	CAATGGCGCCCTCTTGATAA	TGGGAGTCCCTGAGAATTCA	20	20	50.0	50.0	58.3	57.7	26.8	28.7	0.663267	408	1469	1183-1183	1.000000	74.7
psbA_p2	psbA	2	AATGGCGCCCTCTTGATAAA	TGGGAGTCCCTGAGAATTCA	20	20	45.0	50.0	57.6	57.7	26.8	28.7	0.777778	408	1469	1182-1182	1.000000	73.8
psbA_p3	psbA	3	ATGGCGCCCTCTTGATAAAA	TGGGAGTCCCTGAGAATTCA	20	20	45.0	50.0	57.6	57.7	26.8	28.7	0.777778	408	1469	1181-1181	1.000000	73.8
psbA_p4	psbA	4	CAATGGCGCCCTCTTGATAA	AATTCGTGTGCTTGGGAGTC	20	20	50.0	50.0	58.3	58.5	26.8	0.0	0.797103	408	1469	1195-1195	1.000000	73.6
psbA_p5	psbA	5	GGCGCCCTCTTGATAAAACA	TGGGAGTCCCTGAGAATTCA	20	20	50.0	50.0	58.5	57.7	18.1	28.7	0.882956	408	1469	1179-1179	1.000000	72.9
matK_p1	matK	1	CCATGCTACATCAACGTTTCAG	CCCCCAACCCTCTACTTTTT	22	20	45.5	50.0	58.0	57.0	0.0	0.0	3.000129	2023	3537	1672-1672	1.000000	56.0
matK_p2	matK	2	CCATGCTACATCAACGTTTCA	CCCCCAACCCTCTACTTTTT	21	20	42.9	50.0	56.9	57.0	0.0	0.0	3.093600	2023	3537	1672-1672	1.000000	55.3
matK_p3	matK	3	CCATGCTACATCAACGTTTCAG	CCCTGTTTTGACTGTATCGC	22	20	45.5	50.0	58.0	56.8	0.0	0.0	3.190870	2023	3537	1712-1712	1.000000	54.5
matK_p4	matK	4	CCATGCTACATCAACGTTTCAG	CCCCAACCCTCTACTTTTTGT	22	21	45.5	47.6	58.0	57.8	0.0	0.0	3.246070	2023	3537	1671-1671	1.000000	54.0
matK_p5	matK	5	CCATGCTACATCAACGTTTCAG	TGACTGTATCGCACTATGCA	22	20	45.5	45.0	58.0	56.8	0.0	1.2	3.253580	2023	3537	1704-1704	1.000000	54.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	TGAGACCCATCTAGCTCGAA	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.6	58.2	0.0	17.1	0.605505	3538	4258	786-786	1.000000	75.2
matK-trnK-UUU_p2	matK-trnK-UUU	2	TGAGACCCATCTAGCTCGAA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.6	58.2	0.0	17.1	0.605505	3538	4258	785-785	1.000000	75.2
matK-trnK-UUU_p3	matK-trnK-UUU	3	TTGAGACCCATCTAGCTCGA	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.6	58.2	0.0	17.1	0.605505	3538	4258	787-787	1.000000	75.2
matK-trnK-UUU_p4	matK-trnK-UUU	4	TTGAGACCCATCTAGCTCGA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.6	58.2	0.0	17.1	0.605505	3538	4258	786-786	1.000000	75.2
matK-trnK-UUU_p5	matK-trnK-UUU	5	GTTGAGACCCATCTAGCTCG	ACGGTAGAGTACTCGGCTTT	20	20	55.0	50.0	57.5	58.2	0.0	17.1	0.668929	3538	4258	787-787	1.000000	74.6
rps16_p1	rps16	1	GTGTTGGATTGGCACTACAT	TGGACAGCTGAACCAATGAC	20	20	45.0	50.0	56.0	58.1	0.0	25.9	2.086884	4878	5998	1318-1318	1.000000	63.3
rps16_p2	rps16	2	AGGTTTAGGTGGGGAAATCA	TGGACAGCTGAACCAATGAC	20	20	45.0	50.0	56.0	58.1	0.0	25.9	2.098987	4878	5998	1288-1288	1.000000	63.2
rps16_p3	rps16	3	GTGTTGGATTGGCACTACAT	GGACAGCTGAACCAATGACT	20	20	45.0	50.0	56.0	57.8	0.0	23.3	2.174231	4878	5998	1317-1317	1.000000	62.6
rps16_p4	rps16	4	AGGTTTAGGTGGGGAAATCA	GGACAGCTGAACCAATGACT	20	20	45.0	50.0	56.0	57.8	0.0	23.3	2.186335	4878	5998	1287-1287	1.000000	62.5
rps16_p5	rps16	5	AGGTTTAGGTGGGGAAATCAA	TGGACAGCTGAACCAATGAC	21	20	42.9	50.0	56.8	58.1	0.0	25.9	2.304089	4878	5998	1288-1288	1.000000	61.6
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGATCCCT	20	22	45.0	40.9	58.0	57.7	0.0	0.0	2.339278	9088	9817	849-849	1.000000	61.3
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	GCTCGTACAACGGATTAGCA	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	58.1	57.7	0.0	0.0	2.383799	9088	9817	862-862	1.000000	60.9
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	58.2	57.7	0.0	0.0	2.452420	9088	9817	898-898	1.000000	60.4
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AGCTCGTACAACGGATTAGC	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	57.8	57.7	0.0	0.0	2.506379	9088	9817	863-863	1.000000	59.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	TGGAAACGGAAAGAGAGGGA	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	58.3	57.7	0.0	0.0	2.576527	9088	9817	903-903	1.000000	59.4
rpl2_p1	rpl2	1	CGTAGGAACGTCCACGAATT	CCTACTGGATGCATGCCAAT	20	20	50.0	50.0	58.0	58.0	6.0	16.7	0.022181	87090	88577	2497-2498	1.000000	80.4
rpl2_p2	rpl2	2	CGTAGGAACGTCCACGAATT	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	58.0	58.0	6.0	2.3	0.022503	87090	88577	2546-2547	1.000000	80.4
rpl2_p3	rpl2	3	ATAATGGTAGATGCCCGGGA	CCTACTGGATGCATGCCAAT	20	20	50.0	50.0	58.0	58.0	29.3	16.7	0.047742	87090	88577	2147-2148	1.000000	80.3
rpl2_p4	rpl2	4	CGTAGGAACGTCCACGAATT	ACTTCGGGGAAAGGGTAGAA	20	20	50.0	50.0	58.0	58.0	6.0	0.0	0.047738	87090	88577	2158-2159	1.000000	80.2
rpl2_p5	rpl2	5	ATAATGGTAGATGCCCGGGA	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	58.0	58.0	29.3	2.3	0.048064	87090	88577	2196-2197	1.000000	80.2
rpl2-rpl23_p1	rpl2-rpl23	1	TGCTCGGGGTAGAAGTTTTG	CACCTCTTAGAAAGAAAAGAACT	20	23	50.0	34.8	57.8	54.3	0.0	0.0	6.927275	88578	88595	81-81	1.000000	41.0
rpl2-rpl23_p2	rpl2-rpl23	2	GCTCGGGGTAGAAGTTTTGT	CACCTCTTAGAAAGAAAAGAACT	20	23	50.0	34.8	57.8	54.3	0.0	0.0	6.928795	88578	88595	80-80	1.000000	41.0
rpl2-rpl23_p3	rpl2-rpl23	3	GTGCTCGGGGTAGAAGTTTT	CACCTCTTAGAAAGAAAAGAACT	20	23	50.0	34.8	57.8	54.3	0.0	0.0	6.928795	88578	88595	82-82	1.000000	41.0
rpl2-rpl23_p4	rpl2-rpl23	4	TGCTCGGGGTAGAAGTTTTG	CACCTCTTAGAAAGAAAAGAACTT	20	24	50.0	33.3	57.8	55.0	0.0	0.0	7.176741	88578	88595	81-81	1.000000	41.0
rpl2-rpl23_p5	rpl2-rpl23	5	GCTCGGGGTAGAAGTTTTGT	CACCTCTTAGAAAGAAAAGAACTT	20	24	50.0	33.3	57.8	55.0	0.0	0.0	7.178261	88578	88595	80-80	1.000000	41.0
rpl23_p1	rpl23	1	ACCCGTCCTCTTCTCTTTGA	CCTACTGGATGCATGCCAAT	20	20	50.0	50.0	58.0	58.0	0.0	16.7	0.020001	88596	88880	1138-1139	1.000000	81.0
rpl23_p2	rpl23	2	ACCCGTCCTCTTCTCTTTGA	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	58.0	58.0	0.0	2.3	0.020323	88596	88880	1187-1188	1.000000	81.0
rpl23_p3	rpl23	3	CAATTCGCCTCTGACCATCA	CCTACTGGATGCATGCCAAT	20	20	50.0	50.0	58.0	58.0	0.0	16.7	0.037299	88596	88880	1173-1174	1.000000	80.9
rpl23_p4	rpl23	4	CGCCTCTGACCATCAAATGA	CCTACTGGATGCATGCCAAT	20	20	50.0	50.0	58.0	58.0	0.0	16.7	0.037299	88596	88880	1168-1169	1.000000	80.9
rpl23_p5	rpl23	5	TCGCCTCTGACCATCAAATG	CCTACTGGATGCATGCCAAT	20	20	50.0	50.0	58.0	58.0	0.0	16.7	0.037299	88596	88880	1169-1170	1.000000	80.9
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CCGAACGCTCTACCACTAAG	TGGAAGTGGAAGGAAGGGTA	20	20	55.0	50.0	57.8	57.6	0.0	0.0	0.616756	111811	112866	1038-1137	1.000000	79.5
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	CCCCAAGTAGGATTCGAACC	TGGAAGTGGAAGGAAGGGTA	20	20	55.0	50.0	57.7	57.6	0.0	0.0	0.753631	111811	112866	1078-1177	1.000000	78.4
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	CGAACGCTCTACCACTAAGC	TGGAAGTGGAAGGAAGGGTA	20	20	55.0	50.0	58.5	57.6	0.0	0.0	0.879598	111811	112866	1037-1136	1.000000	77.4
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	CCGAACGCTCTACCACTAAG	GGAAGGGTATTATCCACGCA	20	20	55.0	50.0	57.8	57.1	0.0	0.0	1.113064	111811	112866	1027-1126	1.000000	75.5
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	CCGAACGCTCTACCACTAAG	CTGGAAGTGGAAGGAAGGGT	20	20	55.0	55.0	57.8	58.9	0.0	0.0	1.122204	111811	112866	1039-1138	1.000000	75.5
ycf1_p1	ycf1	1	AGTCTTGAATGAGCCCTTTGA	ACCCCGTAACAAATTGGGAC	21	20	42.9	50.0	57.2	58.1	0.0	0.0	1.881910	125920	126919	1105-1105	1.000000	64.9
ycf1_p2	ycf1	2	AGTCTTGAATGAGCCCTTTGA	CCCCGTAACAAATTGGGACT	21	20	42.9	50.0	57.2	57.8	0.0	0.0	2.005378	125920	126919	1104-1104	1.000000	64.0
ycf1_p3	ycf1	3	AGTCTTGAATGAGCCCTTTGA	GACCAATCCACCCAATTCCA	21	20	42.9	50.0	57.2	57.8	0.0	0.0	2.025817	125920	126919	1173-1173	1.000000	63.8
ycf1_p4	ycf1	4	AGTCTTGAATGAGCCCTTTGA	ATTGGGACTGTGAACCTTGG	21	20	42.9	50.0	57.2	57.7	0.0	0.0	2.088516	125920	126919	1093-1093	1.000000	63.3
ycf1_p5	ycf1	5	AGTCTTGAATGAGCCCTTTGA	GTGAACCTTGGTTCTTCCCA	21	20	42.9	50.0	57.2	57.6	0.0	26.6	2.154009	125920	126919	1084-1084	1.000000	62.8
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	TGAAATCGTAGGTGAAATGA	CCGAACGCTCTACCACTAAG	20	20	35.0	55.0	52.2	57.8	0.0	0.0	5.959778	131380	132990	1573-1674	1.000000	44.0
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	AGTCATGAGATTATATTCCTCCCT	CCGAACGCTCTACCACTAAG	24	20	37.5	55.0	56.2	57.8	0.0	0.0	5.979677	131380	132990	1627-1728	1.000000	44.0
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	TGAAATCGTAGGTGAAATGAA	CCGAACGCTCTACCACTAAG	21	20	33.3	55.0	53.2	57.8	0.0	0.0	6.009785	131380	132990	1573-1674	1.000000	44.0
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	TTGAAATCGTAGGTGAAATGA	CCGAACGCTCTACCACTAAG	21	20	33.3	55.0	53.2	57.8	0.0	0.0	6.009785	131380	132990	1574-1675	1.000000	44.0
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	TGAAATCGTAGGTGAAATGA	CCCCAAGTAGGATTCGAACC	20	20	35.0	55.0	52.2	57.7	0.0	0.0	6.096653	131380	132990	1613-1714	1.000000	44.0